MytuD.18758.b

Conserved protein

CENTER ID: MytuD.18758.b
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18758.b.B1.GE37914 full length 1 283
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.3457
RefSeq: NP_217615.1
RefSeq: YP_006516562.1
TubercuList: Rv3099c
MTB Network Portal: Rv3099c
UniProt: O05777

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTPGRPLTT LDKSDVLAGL FAVWHSLDAL LDGLLETDWQ ATSPLPGWDV KAVVSHIIGT ESFLLGIAAP EPDTDVSALA HVRNPIGVMN ECWVRHLGTE SGVGLLERFR AVTSQRRKVL ASLSDDEWNA PTTTPSGPDS YGRFMRIRIF DCWMHEQDIR AAVQRPSSDD ELGGPASPLV LDEIAATMGF VVGKLAKAPD GSRVLLELTG PLSRSIRVSV DGRARVVDDF GGPAPTATIR LDGLQFTRLA GGRPMSPARS QDVELGGDKE LAGHILERLN FVI
NT Sequence
gtgaccacac cgggccgtcc ccttaccaca ctggacaagt ccgacgtgct ggccgggctg ttcgccgttt ggcactccct ggatgcgctg ctcgatggac tgctcgagac ggactggcag gcaacgagtc cgctgcccgg ttgggacgtc aaggccgtgg tgtcgcacat catcggcacc gagtcatttc tgctcggcat cgccgcacca gaaccagaca cagacgtctc tgcgcttgcg catgtccgca accccatcgg cgtgatgaac gagtgctggg tgcgtcatct gggcacagaa tcgggtgtgg gcttgctgga gcggtttcgc gcagtgacta gccagcgccg caaggtctta gcgagccttt ccgatgacga atggaatgcg ccgacgacca ccccctcggg ccccgacagc tacgggcggt tcatgcggat acgcatcttc gactgctgga tgcacgaaca ggacatccgc gcggcggtgc agcgcccgtc gtccgacgac gaactcggcg ggccggcgtc accgctggtc ctcgatgaga tagcggccac catggggttc gtcgtcggca agctggccaa agcgcccgac ggctcccgcg tgctgttgga gctgaccggc ccgctgtctc gcagcatccg cgtttccgtg gacggtcgag cgcgggtggt cgacgacttc ggtgggccgg cgccgacggc gacgatccgc ctggacggac tgcagttcac caggctcgcc ggcgggcgcc cgatgagccc ggcgcgcagc caggatgtgg aactcggcgg cgacaaggag ctggccggtc acatcctcga gcgcctgaac ttcgtgatct ga
Details for MytuD.18758.b.B1.GE37914
HARVESTED ON: 4/10/2014
SEQUENCED ON: 4/8/2014
EXPECTED MW: 32kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV TTPGRPLTTL DKSDVLAGLF AVWHSLDALL DGLLETDWQA TSPLPGWDVK AVVSHIIGTE SFLLGIAAPE PDTDVSALAH VRNPIGVMNE CWVRHLGTES GVGLLERFRA VTSQRRKVLA SLSDDEWNAP TTTPSGPDSY GRFMRIRIFD CWMHEQDIRA AVQRPSSDDE LGGPASPLVL DEIAATMGFV VGKLAKAPDG SRVLLELTGP LSRSIRVSVD GRARVVDDFG GPAPTATIRL DGLQFTRLAG GRPMSPARSQ DVELGGDKEL AGHILERLNF VI
Validated NT Sequence
atcttactca cttagatcac gaagttcagg cgctcgagga tgtgaccggc cagctccttg tcgccgccga gttccacatc ctggctgcgc gccgggctca tcgggcgccc gccggcgagc ctggtgaact gcagtccgtc caggcggatc gtcgccgtcg gcgccggccc accgaagtcg tcgaccaccc gcgctcgacc gtccacggaa acgcggatgc tgcgagacag cgggccggtc agctccaaca gcacgcggga gccgtcgggc gctttggcca gcttgccgac gacgaacccc atggtggccg ctatctcatc gaggaccagc ggtgacgccg gcccgccgag ttcgtcgtcg gacgacgggc gctgcaccgc cgcgcggatg tcctgttcgt gcatccagca gtcgaagatg cgtatccgca tgaaccgccc gtagctgtcg gggcccgagg gggtggtcgt cggcgcattc cattcgtcat cggaaaggct cgctaagacc ttgcggcgct ggctagtcac tgcgcgaaac cgctccagca agcccacacc cgattctgtg cccagatgac gcacccagca ctcgttcatc acgccgatgg ggttgcggac atgcgcaagc gcagagacgt ctgtgtctgg ttctggtgcg gcgatgccga gcagaaatga ctcggtgccg atgatgtgcg acaccacggc cttgacgtcc caaccgggca gcggactcgt tgcctgccag tccgtctcga gcagtccatc gagcagcgca tccagggagt gccaaacggc gaacagcccg gccagcacgt cggacttgtc cagtgtggta aggggacggc ccggtgtggt caccatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMT TPGRPLTTLD KSDVLAGLFA VWHSLDALLD GLLETDWQAT SPLPGWDVKA VVSHIIGTES FLLGIAAPEP DTDVSALAHV RNPIGVMNEC WVRHLGTESG VGLLERFRAV TSQRRKVLAS LSDDEWNAPT TTPSGPDSYG RFMRIRIFDC WMHEQDIRAA VQRPSSDDEL GGPASPLVLD EIAATMGFVV GKLAKAPDGS RVLLELTGPL SRSIRVSVDG RARVVDDFGG PAPTATIRLD GLQFTRLAGG RPMSPARSQD VELGGDKELA GHILERLNFV I
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgacca caccgggccg tccccttacc acactggaca agtccgacgt gctggccggg ctgttcgccg tttggcactc cctggatgcg ctgctcgatg gactgctcga gacggactgg caggcaacga gtccgctgcc cggttgggac gtcaaggccg tggtgtcgca catcatcggc accgagtcat ttctgctcgg catcgccgca ccagaaccag acacagacgt ctctgcgctt gcgcatgtcc gcaaccccat cggcgtgatg aacgagtgct gggtgcgtca tctgggcaca gaatcgggtg tgggcttgct ggagcggttt cgcgcagtga ctagccagcg ccgcaaggtc ttagcgagcc tttccgatga cgaatggaat gcgccgacga ccaccccctc gggccccgac agctacgggc ggttcatgcg gatacgcatc ttcgactgct ggatgcacga acaggacatc cgcgcggcgg tgcagcgccc gtcgtccgac gacgaactcg gcgggccggc gtcaccgctg gtcctcgatg agatagcggc caccatgggg ttcgtcgtcg gcaagctggc caaagcgccc gacggctccc gcgtgctgtt ggagctgacc ggcccgctgt ctcgcagcat ccgcgtttcc gtggacggtc gagcgcgggt ggtcgacgac ttcggtgggc cggcgccgac ggcgacgatc cgcctggacg gactgcagtt caccaggctc gccggcgggc gcccgatgag cccggcgcgc agccaggatg tggaactcgg cggcgacaag gagctggccg gtcacatcct cgagcgcctg aacttcgtga tctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat