MytuD.18693.a

CRISPR-associated endonuclease Cas1 (EC 3.1.-.-)

CENTER ID: MytuD.18693.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18693.a.B1.GE37243 full length 1 338
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.3142
RefSeq: NP_217333.1
RefSeq: YP_006516266.1
TubercuList: Rv2817c
MTB Network Portal: Rv2817c
UniProt: P9WPJ5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVQLYVSDSV SRISFADGRV IVWSEELGES QYPIETLDGI TLFGRPTMTT PFIVEMLKRE RDIQLFTTDG HYQGRISTPD VSYAPRLRQQ VHRTDDPAFC LSLSKRIVSR KILNQQALIR AHTSGQDVAE SIRTMKHSLA WVDRSGSLAE LNGFEGNAAK AYFTALGHLV PQEFAFQGRS TRPPLDAFNS MVSLGYSLLY KNIIGAIERH SLNAYIGFLH QDSRGHATLA SDLMEVWRAP IIDDTVLRLI ADGVVDTRAF SKNSDTGAVF ATREATRSIA RAFGNRIART ATYIKGDPHR YTFQYALDLQ LQSLVRVIEA GHPSRLVDID ITSEPSGA
NT Sequence
atggtgcagc tgtatgtctc ggactccgtg tcgcggatca gctttgccga cggccgggtg atcgtgtgga gcgaggagct cggcgagagc cagtatccga tcgagacgct ggacggcatc acgctgtttg ggcggccgac gatgacaacg cccttcatcg ttgagatgct caagcgtgag cgcgacatcc agctcttcac gaccgacggc cactaccagg gccggatctc aacacccgac gtgtcatacg cgccgcggct ccgtcagcaa gttcaccgca ccgacgatcc tgcgttctgc ctgtcgttaa gcaagcggat cgtgtcgagg aagatcctga atcagcaggc cttgattcgg gcacacacgt cggggcaaga cgttgctgag agcatccgca cgatgaagca ctcgctggcc tgggtcgatc gatcgggctc cctggcggag ttgaacgggt tcgagggaaa tgccgcaaag gcatacttca ccgcgctggg gcatctcgtc ccgcaggagt tcgcattcca gggccgctcg actcggccgc cgttggacgc cttcaactcg atggtcagcc tcggctattc gctgctgtac aagaacatca taggggcgat cgagcgtcac agcctgaacg cgtatatcgg tttcctacac caggattcac gagggcacgc aacgttggcg agcgacctca tggaggtatg gcgggcgccg atcatcgatg acaccgtact tcgattgatc gcggacggtg tggtcgacac ccgggctttc agcaagaact ccgacacggg ggccgtcttc gcgacacggg aagccacacg atccatcgcg cgcgcctttg gtaatcgaat cgcacgaacc gccacctaca tcaaaggcga tcctcaccga tacacctttc agtacgccct cgacttgcaa ctgcaaagcc tcgtgcgtgt tatcgaagcc gggcacccgt cgcggctcgt cgatatcgat atcacctccg agccatccgg agcctaa
Details for MytuD.18693.a.B1.GE37243
HARVESTED ON: 8/13/2013
SEQUENCED ON: 8/15/2013
EXPECTED MW: 39kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 88
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV QLYVSDSVSR ISFADGRVIV WSEELGESQY PIETLDGITL FGRPTMTTPF IVEMLKRERD IQLFTTDGHY QGRISTPDVS YAPRLRQQVH RTDDPAFCLS LSKRIVSRKI LNQQALIRAH TSGQDVAESI RTMKHSLAWV DRSGSLAELN GFEGNAAKAY FTALGHLVPQ EFAFQGRSTR PPLDAFNSMV SLGYSLLYKN IIGAIERHSL NAYIGFLHQD SRGHATLASD LMEVWRAPII DDTVLRLIAD GVVDTRAFSK NSDTGAVFAT REATRSIARA FGNRIARTAX YIKGXPXXTX FSTPXTCXXK XXRXSXXXXX XXXXXXXXXX XXXXXXXXX
Validated NT Sequence
attttgtnnn nnttaagaag gagatatacc atggctcacc accaccacca ccatatggtg cagctgtatg tctcggactc cgtgtcgcgg atcagctttg ccgacggccg ggtgatcgtg tggagcgagg agctcggcga gagccagtat ccgatcgaga cgctggacgg catcacgctg tttgggcggc cgacgatgac aacgcccttc atcgttgaga tgctcaagcg tgagcgcgac atccagctct tcacgaccga cggccactac cagggccgga tctcaacacc cgacgtgtca tacgcgccgc ggctccgtca gcaagttcac cgcaccgacg atcctgcgtt ctgcctgtcg ttaagcaagc ggatcgtgtc gaggaagatc ctgaatcagc aggccttgat tcgggcacac acgtcggggc aagacgttgc tgagagcatc cgcacgatga agcactcgct ggcctgggtc gatcgatcgg gctccctggc ggagttgaac gggttcgagg gaaatgccgc aaaggcatac ttcaccgcgc tggggcatct cgtcccgcag gagttcgcat tccagggccg ctcgactcgg ccgccgttgg acgccttcaa ctcgatggtc agcctcggct attcgctgct gtacaagaac atcatagggg cgatcgagcg tcacagcctg aacgcgtata tcggtttcct acaccaggat tcacgagggc acgcaacgtt ggcgagcgac ctcatggagg tatggcgggc gccgatcatc gatgacaccg tacttcgatt gatcgcggac ggtgtggtcg acacccgggc tttcagcaag aactccgaca cgggggccgt cttcgcgaca cgggaagcca cacgatccat cgcgcgcgcc tttggtaatc gaatcgcacg aaccgcnnnc tacatcaaag gnnatcctcn cnatacnnct ttcagtacgc ccncnacttg canctgnaag cncntgcgnn tntcnannnn nnncnntcnn nnnngncnan tcganntcnc tcnnncntnn nnctanngan tannnnnnnc gnnnnnnna
Expected Protein Sequence
MAHHHHHHMV QLYVSDSVSR ISFADGRVIV WSEELGESQY PIETLDGITL FGRPTMTTPF IVEMLKRERD IQLFTTDGHY QGRISTPDVS YAPRLRQQVH RTDDPAFCLS LSKRIVSRKI LNQQALIRAH TSGQDVAESI RTMKHSLAWV DRSGSLAELN GFEGNAAKAY FTALGHLVPQ EFAFQGRSTR PPLDAFNSMV SLGYSLLYKN IIGAIERHSL NAYIGFLHQD SRGHATLASD LMEVWRAPII DDTVLRLIAD GVVDTRAFSK NSDTGAVFAT REATRSIARA FGNRIARTAT YIKGDPHRYT FQYALDLQLQ SLVRVIEAGH PSRLVDIDIT SEPSGA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtgc agctgtatgt ctcggactcc gtgtcgcgga tcagctttgc cgacggccgg gtgatcgtgt ggagcgagga gctcggcgag agccagtatc cgatcgagac gctggacggc atcacgctgt ttgggcggcc gacgatgaca acgcccttca tcgttgagat gctcaagcgt gagcgcgaca tccagctctt cacgaccgac ggccactacc agggccggat ctcaacaccc gacgtgtcat acgcgccgcg gctccgtcag caagttcacc gcaccgacga tcctgcgttc tgcctgtcgt taagcaagcg gatcgtgtcg aggaagatcc tgaatcagca ggccttgatt cgggcacaca cgtcggggca agacgttgct gagagcatcc gcacgatgaa gcactcgctg gcctgggtcg atcgatcggg ctccctggcg gagttgaacg ggttcgaggg aaatgccgca aaggcatact tcaccgcgct ggggcatctc gtcccgcagg agttcgcatt ccagggccgc tcgactcggc cgccgttgga cgccttcaac tcgatggtca gcctcggcta ttcgctgctg tacaagaaca tcataggggc gatcgagcgt cacagcctga acgcgtatat cggtttccta caccaggatt cacgagggca cgcaacgttg gcgagcgacc tcatggaggt atggcgggcg ccgatcatcg atgacaccgt acttcgattg atcgcggacg gtgtggtcga cacccgggct ttcagcaaga actccgacac gggggccgtc ttcgcgacac gggaagccac acgatccatc gcgcgcgcct ttggtaatcg aatcgcacga accgccacct acatcaaagg cgatcctcac cgatacacct ttcagtacgc cctcgacttg caactgcaaa gcctcgtgcg tgttatcgaa gccgggcacc cgtcgcggct cgtcgatatc gatatcacct ccgagccatc cggagcctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at