SSGCID: Target Page

MytuD.18692.a

Putative uncharacterized protein

CENTER ID:	MytuD.18692.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.18692.a.B2.GE37241	soluble domain 1		64	322
MytuD.18692.a.B3.GE37242	soluble domain 2		344	876

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.18692.a.B2.PS01928	soluble domain 1		64	322

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|83332.12.peg.3759
RefSeq:	NP_217882.1
RefSeq:	YP_006516847.1
TubercuList:	Rv3365c
MTB Network Portal:	Rv3365c
UniProt:	Q93IG6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTMFARPTIP VAAAASDISA PAQPARGKPQ QRPPSWSPRN WPVRWKVFTI ALLPLVVAMV LAGLRVEAAM ASTSGLRLVA ARAEMIPAIT KYMSALDVAV LASSTGHDVE GAQKNFTARK YELQTRLADT DVIADVRSGV NTLLNGGQAL LDKVLADSIG LRDRVTAYAP LLLTAQNVID ASVRVDSEQI RTQVQGLSRA VGARGQMTMQ EILVTRGADL AEPQLRSAMV TLAGTEPSTL FGMSAALGAG SPDTKNLQQQ MVTRMAIMSD PAVALVNNPE LLHSIQITRD IAEQVITDTT EAVTKSVQSQ ATDRRDAAIR DAVLVLAAIA TAIVVVLVVA RTLVGPMRVL RDGALKVAHT DLDGEIAAVR AGDEPIPEPL AVYTTEEIGQ VAHAVDELHT RALLLAGEET RLRLLVNEMF ETMSRRSRSL VDQQLSVIDQ LERNEEDPAR LDSLFRLDHL AARLRRNSAN LLVLAGAQIT RDHREPVPLS TVISAAVSEV EDYRRVDIAR VPDCAVVGAA AGGVIHLLAE LIDNALRYSS PTTPVRVAAA IGSEGSVLLR ISDSGLGMTD ADRRMANMRL RAGGEVTPDS ARHMGLFVVG RLAGRHGIRV GLRGPVTGEQ GTGTTAEVYL PLAVLEGTAP AQPPKPRVFA IKPPCPEPAA ADPTDVPAAI GPLPPVTLLP RRTPGSSGIA DVPAQPMQQR RRELKTPWWE DRFQQEPKQP PAPEPRPAPP PAKPAPPAGP VDDDVIYRRM LSEMVGDPHE LAHSPDLDWK SVWDHGWSAA AEAADKPVQS RTDYGLPVRE PGARLVPGAA VPEGPDREHP GAALASNGGL HPGRAPRHAA AVRDPDAVRA SISSHFGGVR TGRSHARESS QGPNQQ

NT Sequence

gtgaccatgt tcgcccgccc gaccatcccg gtcgcggcgg ccgcttctga tatttccgcc ccggctcaac cggcccgcgg caaacctcag caacgcccgc cgtcctggtc gccgcgcaac tggccggtcc gatggaaagt gttcacgatc gcgcttctgc cgctggtagt ggcgatggtg ttagcaggat tgcgggtcga ggctgcgatg gccagcacca gcggcctgcg gctggtcgcc gcgcgcgccg aaatgatacc cgcgatcacg aaatacatgt cggcgctgga cgtcgccgtg ctggccagct cgaccggaca cgatgtggag ggggcgcaga aaaacttcac cgcccgcaag tacgagctgc agacgcgact ggccgacacc gacgtcatcg cagacgtgcg gtcgggagtg aacacgctgc tcaacggcgg tcaggcgctg ctggataagg tgctggccga cagcatcggc ttgcgggatc gggtcaccgc ctacgcgccg ctgctgttga cggcccagaa cgtgattgac gcgtcggtgc gggttgacag cgagcaaatc cgaacccagg tgcagggttt gagccgagcc gttggcgccc gcgggcagat gacgatgcag gagatcctgg tgactcgcgg cgccgacctt gccgagccgc aactgcgcag cgcgatggtt accctggccg gcaccgaacc ctcgacgctg ttcgggatga gcgcggcgct cggtgcaggc tcgccggaca ccaagaacct gcagcagcaa atggtgacca ggatggcgat catgtccgat ccggccgttg cactggtcaa caacccagag ctgctgcact cgatacagat cacccgcgac attgccgagc aggtgatcac cgacaccacc gaggcggtga cgaagtcggt gcaaagccag gccaccgacc ggcgggatgc cgcgattcgc gacgccgtgc tggtgttggc cgccatcgcg accgcgatcg tcgtcgtgtt ggtggtggcg cgcacgctgg tcgggccgat gcgggtactg cgtgatgggg cgctcaaggt tgctcatacc gatctcgacg gcgagatcgc ggcggtccgc gccggcgacg agccgatccc cgagccactg gcggtgtaca ccaccgagga aatcggtcag gtcgcgcatg cggtcgacga gctgcacacc cgggccctgt tgctggccgg cgaggaaacg cggttgcgac tgctggtcaa cgagatgttt gagaccatgt cgcggcgtag ccgttccctg gtcgaccagc agctgtcggt catcgaccaa ctggagcgca acgaggagga tcccgcccga ctcgacagcc ttttccggct cgatcacctg gccgcccggc tgcgccgcaa cagcgccaac ctgctggtgc tggccggtgc gcagattacc cgtgaccacc gcgagccggt gccgctgtca accgtgatca gcgccgccgt gtcagaggtc gaggactatc gccgcgtcga catcgcgagg gtacccgact gtgcggtagt cggcgcagcg gctggtggcg tcattcatct gcttgccgag ctgatcgaca acgcgttgcg ctactcgtca ccgaccacac ccgttcgggt tgccgccgca atcggcagcg aaggcagtgt tctgctgcga atctcggatt ccggcctggg catgaccgat gccgatcggc ggatggccaa tatgcggctg cgggccggcg gtgaggtcac cccggatagt gcccggcaca tgggtctgtt cgtagtcggc cggctggccg gtcggcacgg catccgagtc gggctgcgcg gtccggtgac cggtgaacag ggcaccggca ccaccgccga ggtctacctg ccgctagccg tgctcgaggg gacggcccca gcgcagccgc caaagccgcg ggtatttgcg atcaagccgc cgtgtcctga acccgcggcg gccgatccga cggacgttcc cgccgccatc gggccgctac caccggtcac gttgctcccg cgccgtaccc cggggtccag tggcatcgcc gacgtcccgg cccagccgat gcagcagcgg cggcgcgagc tgaaaacacc ctggtgggag gataggtttc aacaggagcc caaacaaccg cccgcaccag aaccgcgacc ggcgccgccg cccgccaaac ccgcgccacc ggcgggcccg gttgatgacg acgtcatcta ccggcggatg ctctccgaga tggtgggtga cccgcacgag ctggcccaca gccccgatct ggactggaag tcggtgtggg accacggctg gtcggcggcc gccgaggccg cggacaagcc cgtgcagtcc cgcacggact acggcctgcc ggtgcgcgaa cccggggccc ggttagtgcc gggggcggcg gtgcctgagg gacccgatcg ggagcatccg ggtgcagcgc tagcatccaa cggcggactt catcccggcc gagcgccgcg gcacgcggct gcggtacgcg accccgacgc ggttcgtgcc tccatcagca gccatttcgg cggcgtgcgc accgggcggt cgcatgcccg cgagagcagt cagggaccca atcagcaatg a

Details for MytuD.18692.a.B2.GE37241

HARVESTED ON:	8/13/2013
SEQUENCED ON:	8/15/2013
EXPECTED MW:	29kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML RVEAAMASTS GLRLVAARAE MIPAITKYMS ALDVAVLASS TGHDVEGAQK NFTARKYELQ TRLADTDVIA DVRSGVNTLL NGGQALLDKV LADSIGLRDR VTAYAPLLLT AQNVIDASVR VDSEQIRTQV QGLSRAVGAR GQMTMQEILV TRGADLAEPQ LRSAMVTLAG TEPSTLFGMS AALGAGSPDT KNLQQQMVTR MAIMSDPAVA LVNNPELLHS IQITRDIAEQ VITDTTEAVT KSVQSQATDR RDAAIRDA

Validated NT Sequence

atcttactca cttaggcgtc gcgaatcgcg gcatcccgcc ggtcggtggc ctggctttgc accgacttcg tcaccgcctc ggtggtgtcg gtgatcacct gctcggcaat gtcgcgggtg atctgtatcg agtgcagcag ctctgggttg ttgaccagtg caacggccgg atcggacatg atcgccatcc tggtcaccat ttgctgctgc aggttcttgg tgtccggcga gcctgcaccg agcgccgcgc tcatcccgaa cagcgtcgag ggttcggtgc cggccagggt aaccatcgcg ctgcgcagtt gcggctcggc aaggtcggcg ccgcgagtca ccaggatctc ctgcatcgtc atctgcccgc gggcgccaac ggctcggctc aaaccctgca cctgggttcg gatttgctcg ctgtcaaccc gcaccgacgc gtcaatcacg ttctgggccg tcaacagcag cggcgcgtag gcggtgaccc gatcccgcaa gccgatgctg tcggccagca ccttatccag cagcgcctga ccgccgttga gcagcgtgtt cactcccgac cgcacgtctg cgatgacgtc ggtgtcggcc agtcgcgtct gcagctcgta cttgcgggcg gtgaagtttt tctgcgcccc ctccacatcg tgtccggtcg agctggccag cacggcgacg tccagcgccg acatgtattt cgtgatcgcg ggtatcattt cggcgcgcgc ggcgaccagc cgcaggccgc tggtgctggc catcgcagcc tcgacccgca acatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

MAHHHHHHLR VEAAMASTSG LRLVAARAEM IPAITKYMSA LDVAVLASST GHDVEGAQKN FTARKYELQT RLADTDVIAD VRSGVNTLLN GGQALLDKVL ADSIGLRDRV TAYAPLLLTA QNVIDASVRV DSEQIRTQVQ GLSRAVGARG QMTMQEILVT RGADLAEPQL RSAMVTLAGT EPSTLFGMSA ALGAGSPDTK NLQQQMVTRM AIMSDPAVAL VNNPELLHSI QITRDIAEQV ITDTTEAVTK SVQSQATDRR DAAIRDA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgcggg tcgaggctgc gatggccagc accagcggcc tgcggctggt cgccgcgcgc gccgaaatga tacccgcgat cacgaaatac atgtcggcgc tggacgtcgc cgtgctggcc agctcgaccg gacacgatgt ggagggggcg cagaaaaact tcaccgcccg caagtacgag ctgcagacgc gactggccga caccgacgtc atcgcagacg tgcggtcggg agtgaacacg ctgctcaacg gcggtcaggc gctgctggat aaggtgctgg ccgacagcat cggcttgcgg gatcgggtca ccgcctacgc gccgctgctg ttgacggccc agaacgtgat tgacgcgtcg gtgcgggttg acagcgagca aatccgaacc caggtgcagg gtttgagccg agccgttggc gcccgcgggc agatgacgat gcaggagatc ctggtgactc gcggcgccga ccttgccgag ccgcaactgc gcagcgcgat ggttaccctg gccggcaccg aaccctcgac gctgttcggg atgagcgcgg cgctcggtgc aggctcgccg gacaccaaga acctgcagca gcaaatggtg accaggatgg cgatcatgtc cgatccggcc gttgcactgg tcaacaaccc agagctgctg cactcgatac agatcacccg cgacattgcc gagcaggtga tcaccgacac caccgaggcg gtgacgaagt cggtgcaaag ccaggccacc gaccggcggg atgccgcgat tcgcgacgcc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat

Details for MytuD.18692.a.B3.GE37242

HARVESTED ON:	8/13/2013
SEQUENCED ON:	8/15/2013
EXPECTED MW:	58kDa
OBSERVED MW:	64kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MRVLRDGALK VAHTDLDGEI AAVRAGDEPI PEPLAVYTTE EIGQVAHAVD ELHTRALLLA GEETRLRLLV NEMFETMSRR SRSLVDQQLS VIDQLERNEE DPARLDSLFR LDHLAARLRR NSANLLVLAG AQITRDHREP VPLSTVISAA VSEVEDYRRV DIARVPDCAV VGAAAGGVIH LLAELIDNAL RYSSPTTPVR VAAAIGSEGS VLLRISDSGL GMTDADRRMA NMRLRAGGEV TPDSARHMGL FVVGRLAGRH GIRVGLRGPV TGEQGTGTTA EVYLPLAVLE GTAPAQPPKP RVFAIKPPCP EPAAADPTDV PAAIGPLPPV TLLPRRTPGS SGIADVPAQP MQQRRRELKT PWWEDRFQQE PKQPPAPEPR PAPPPAKPAP PAGPVDDDVI YRRMLSEMVG DPHELAHSPD LDWKSVWDHG WSAAAEAADK PVQSRTDYGL PVREPGARLV PGAAVPEGPD REHPGAALAS NGGLHPGRAP RHAAAVRDPD AVRASISSHF GGVRTGRSHA RESSQGPNQQ

Validated NT Sequence

atcttactca cttattgctg attgggtccc tgactgctct cgcgggcatg cgaccgcccg gtgcgcacgc cgccgaaatg gctgctgatg gaggcacgaa ccgcgtcggg gtcgcgtacc gcagccgcgt gccgcggcgc tcggccggga tgaagtccgc cgttggatgc tagcgctgca cccggatgct cccgatcggg tccctcaggc accgccgccc ccggcactaa ccgggccccg ggttcgcgca ccggcaggcc gtagtccgtg cgggactgca cgggcttgtc cgcggcctcg gcggccgccg accagccgtg gtcccacacc gacttccagt ccagatcggg gctgtgggcc agctcgtgcg ggtcacccac catctcggag agcatccgcc ggtagatgac gtcgtcatca accgggcccg ccggtggcgc gggtttggcg ggcggcggcg ccggtcgcgg ttctggtgcg ggcggttgtt tgggctcctg ttgaaaccta tcctcccacc agggtgtttt cagctcgcgc cgccgctgct gcatcggctg ggccgggacg tcggcgatgc cactggaccc cggggtacgg cgcgggagca acgtgaccgg tggtagcggc ccgatggcgg cgggaacgtc cgtcggatcg gccgccgcgg gttcaggaca cggcggcttg atcgcaaata cccgcggctt tggcggctgc gctggggccg tcccctcgag cacggctagc ggcaggtaga cctcggcggt ggtgccggtg ccctgttcac cggtcaccgg accgcgcagc ccgactcgga tgccgtgccg accggccagc cggccgacta cgaacagacc catgtgccgg gcactatccg gggtgacctc accgccggcc cgcagccgca tattggccat ccgccgatcg gcatcggtca tgcccaggcc ggaatccgag attcgcagca gaacactgcc ttcgctgccg attgcggcgg caacccgaac gggtgtggtc ggtgacgagt agcgcaacgc gttgtcgatc agctcggcaa gcagatgaat gacgccacca gccgctgcgc cgactaccgc acagtcgggt accctcgcga tgtcgacgcg gcgatagtcc tcgacctctg acacggcggc gctgatcacg gttgacagcg gcaccggctc gcggtggtca cgggtaatct gcgcaccggc cagcaccagc aggttggcgc tgttgcggcg cagccgggcg gccaggtgat cgagccggaa aaggctgtcg agtcgggcgg gatcctcctc gttgcgctcc agttggtcga tgaccgacag ctgctggtcg accagggaac ggctacgccg cgacatggtc tcaaacatct cgttgaccag cagtcgcaac cgcgtttcct cgccggccag caacagggcc cgggtgtgca gctcgtcgac cgcatgcgcg acctgaccga tttcctcggt ggtgtacacc gccagtggct cggggatcgg ctcgtcgccg gcgcggaccg ccgcgatctc gccgtcgaga tcggtatgag caaccttgag cgccccatca cgcagtaccc gcatcggcc

Expected Protein Sequence

MAHHHHHHVG PMRVLRDGAL KVAHTDLDGE IAAVRAGDEP IPEPLAVYTT EEIGQVAHAV DELHTRALLL AGEETRLRLL VNEMFETMSR RSRSLVDQQL SVIDQLERNE EDPARLDSLF RLDHLAARLR RNSANLLVLA GAQITRDHRE PVPLSTVISA AVSEVEDYRR VDIARVPDCA VVGAAAGGVI HLLAELIDNA LRYSSPTTPV RVAAAIGSEG SVLLRISDSG LGMTDADRRM ANMRLRAGGE VTPDSARHMG LFVVGRLAGR HGIRVGLRGP VTGEQGTGTT AEVYLPLAVL EGTAPAQPPK PRVFAIKPPC PEPAAADPTD VPAAIGPLPP VTLLPRRTPG SSGIADVPAQ PMQQRRRELK TPWWEDRFQQ EPKQPPAPEP RPAPPPAKPA PPAGPVDDDV IYRRMLSEMV GDPHELAHSP DLDWKSVWDH GWSAAAEAAD KPVQSRTDYG LPVREPGARL VPGAAVPEGP DREHPGAALA SNGGLHPGRA PRHAAAVRDP DAVRASISSH FGGVRTGRSH ARESSQGPNQ Q

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtcgggc cgatgcgggt actgcgtgat ggggcgctca aggttgctca taccgatctc gacggcgaga tcgcggcggt ccgcgccggc gacgagccga tccccgagcc actggcggtg tacaccaccg aggaaatcgg tcaggtcgcg catgcggtcg acgagctgca cacccgggcc ctgttgctgg ccggcgagga aacgcggttg cgactgctgg tcaacgagat gtttgagacc atgtcgcggc gtagccgttc cctggtcgac cagcagctgt cggtcatcga ccaactggag cgcaacgagg aggatcccgc ccgactcgac agccttttcc ggctcgatca cctggccgcc cggctgcgcc gcaacagcgc caacctgctg gtgctggccg gtgcgcagat tacccgtgac caccgcgagc cggtgccgct gtcaaccgtg atcagcgccg ccgtgtcaga ggtcgaggac tatcgccgcg tcgacatcgc gagggtaccc gactgtgcgg tagtcggcgc agcggctggt ggcgtcattc atctgcttgc cgagctgatc gacaacgcgt tgcgctactc gtcaccgacc acacccgttc gggttgccgc cgcaatcggc agcgaaggca gtgttctgct gcgaatctcg gattccggcc tgggcatgac cgatgccgat cggcggatgg ccaatatgcg gctgcgggcc ggcggtgagg tcaccccgga tagtgcccgg cacatgggtc tgttcgtagt cggccggctg gccggtcggc acggcatccg agtcgggctg cgcggtccgg tgaccggtga acagggcacc ggcaccaccg ccgaggtcta cctgccgcta gccgtgctcg aggggacggc cccagcgcag ccgccaaagc cgcgggtatt tgcgatcaag ccgccgtgtc ctgaacccgc ggcggccgat ccgacggacg ttcccgccgc catcgggccg ctaccaccgg tcacgttgct cccgcgccgt accccggggt ccagtggcat cgccgacgtc ccggcccagc cgatgcagca gcggcggcgc gagctgaaaa caccctggtg ggaggatagg tttcaacagg agcccaaaca accgcccgca ccagaaccgc gaccggcgcc gccgcccgcc aaacccgcgc caccggcggg cccggttgat gacgacgtca tctaccggcg gatgctctcc gagatggtgg gtgacccgca cgagctggcc cacagccccg atctggactg gaagtcggtg tgggaccacg gctggtcggc ggccgccgag gccgcggaca agcccgtgca gtcccgcacg gactacggcc tgccggtgcg cgaacccggg gcccggttag tgccgggggc ggcggtgcct gagggacccg atcgggagca tccgggtgca gcgctagcat ccaacggcgg acttcatccc ggccgagcgc cgcggcacgc ggctgcggta cgcgaccccg acgcggttcg tgcctccatc agcagccatt tcggcggcgt gcgcaccggg cggtcgcatg cccgcgagag cagtcaggga cccaatcagc aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for MytuD.18692.a.B2.PS01928

PURIFICATION DATe:	11/6/2013
CONCENTRATION:	52mg/ml
OBSERVED MW:	27kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	1
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTTGCGGG TCGAGGCTGC GATGGCCAGC ACCAGCGGCC TGCGGCTGGT CGCCGCGCGC GCCGAAATGA TACCCGCGAT CACGAAATAC ATGTCGGCGC TGGACGTCGC CGTGCTGGCC AGCTCGACCG GACACGATGT GGAGGGGGCG CAGAAAAACT TCACCGCCCG CAAGTACGAG CTGCAGACGC GACTGGCCGA CACCGACGTC ATCGCAGACG TGCGGTCGGG AGTGAACACG CTGCTCAACG GCGGTCAGGC GCTGCTGGAT AAGGTGCTGG CCGACAGCAT CGGCTTGCGG GATCGGGTCA CCGCCTACGC GCCGCTGCTG TTGACGGCCC AGAACGTGAT TGACGCGTCG GTGCGGGTTG ACAGCGAGCA AATCCGAACC CAGGTGCAGG GTTTGAGCCG AGCCGTTGGC GCCCGCGGGC AGATGACGAT GCAGGAGATC CTGGTGACTC GCGGCGCCGA CCTTGCCGAG CCGCAACTGC GCAGCGCGAT GGTTACCCTG GCCGGCACCG AACCCTCGAC GCTGTTCGGG ATGAGCGCGG CGCTCGGTGC AGGCTCGCCG GACACCAAGA ACCTGCAGCA GCAAATGGTG ACCAGGATGG CGATCATGTC CGATCCGGCC GTTGCACTGG TCAACAACCC AGAGCTGCTG CACTCGATAC AGATCACCCG CGACATTGCC GAGCAGGTGA TCACCGACAC CACCGAGGCG GTGACGAAGT CGGTGCAAAG CCAGGCCACC GACCGGCGGG ATGCCGCGAT TCGCGACGCC TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT