SSGCID: Target Page

MytuD.18691.a

Uncharacterized protein Rv1339/MT1380

CENTER ID:	MytuD.18691.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.18691.a.B1.GE37240	full length		1	273

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|83332.12.peg.1500
RefSeq:	NP_215855.1
RefSeq:	YP_006514717.1
TubercuList:	Rv1339
MTB Network Portal:	Rv1339
UniProt:	P9WGC1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRRCIPHRCI GHGTVVSVRI TVLGCSGSVV GPDSPASGYL LRAPHTPPLV IDFGGGVLGA LQRHADPASV HVLLSHLHAD HCLDLPGLFV WRRYHPSRPS GKALLYGPSD TWSRLGAASS PYGGEIDDCS DIFDVHHWAD SEPVTLGALT IVPRLVAHPT ESFGLRITDP SGASLAYSGD TGICDQLVEL ARGVDVFLCE ASWTHSPKHP PDLHLSGTEA GMVAAQAGVR ELLLTHIPPW TSREDVISEA KAEFDGPVHA VVCDETFEVR RAG

NT Sequence

atgcgtcgat gtattccgca tcgttgtatc gggcatggca cagtagtgtc cgtgcggata accgtgctcg gatgctccgg tagcgtcgtg gggccggatt cgcctgcgtc ggggtatttg ctccgagcgc cgcacacacc gccgttggtt atcgacttcg gcgggggtgt gctcggcgcg ctgcaacggc acgcggatcc cgcgtcggtg catgtgctgc tgtcgcatct gcatgcggac cattgtctgg acttgccggg actttttgtg tggcggcgtt accacccgtc gcgtccctct ggcaaggcat tgttgtacgg ccccagcgac acctggtcgc gattgggggc ggcgtcgtcc ccgtacggtg gggagattga cgactgttcg gatatcttcg atgttcacca ctgggccgac agtgagccag tgacgttggg cgcccttacg atagtgccgc ggctggttgc ccacccgact gagtcgtttg gcctgcggat caccgatccg agcggtgcgt cactggctta tagcggcgac accggcattt gtgaccagct cgtcgagctg gctcgcggcg tcgacgtttt cctctgcgag gcctcctgga cacactcgcc caaacatcca cccgatctac acctgtcggg caccgaagcc ggtatggttg ccgcgcaagc cggcgttcgt gagctgctgc tgacgcatat cccgccgtgg acttcgcgtg aggacgtcat cagcgaggcc aaggccgagt tcgacggccc ggtgcacgcg gtggtatgcg acgagacgtt cgaagtccgg cgagccggct ag

Details for MytuD.18691.a.B1.GE37240

HARVESTED ON:	8/13/2013
SEQUENCED ON:	8/15/2013
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMR RCIPHRCIGH GTVVSVRITV LGCSGSVVGP DSPASGYLLR APHTPPLVID FGGGVLGALQ RHADPASVHV LLSHLHADHC LDLPGLFVWR RYHPSRPSGK ALLYGPSDTW SRLGAASSPY GGEIDDCSDI FDVHHWADSE PVTLGALTIV PRLVAHPTES FGLRITDPSG ASLAYSGDTG ICDQLVELAR GVDVFLCEAS WTHSPKHPPD LHLSGTEAGM VAAQAGVREL LLTHIPPWTS REDVISEAKA XFDGPVHAVV CDETFEVRRA G

Validated NT Sequence

ttntnnnntt taagaaggag anataccatg gctcaccacc accaccacca tatgcgtcga tgtattccgc atcgttgtat cgggcatggc acagtagtgt ccgtgcggat aaccgtgctc ggatgctccg gtagcgtcgt ggggccggat tcgcctgcgt cggggtattt gctccgagcg ccgcacacac cgccgttggt tatcgacttc ggcgggggtg tgctcggcgc gctgcaacgg cacgcggatc ccgcgtcggt gcatgtgctg ctgtcgcatc tgcatgcgga ccattgtctg gacttgccgg gactttttgt gtggcggcgt taccacccgt cgcgtccctc tggcaaggca ttgttgtacg gccccagcga cacctggtcg cgattggggg cggcgtcgtc cccgtacggt ggggagattg acgactgttc ggatatcttc gatgttcacc actgggccga cagtgagcca gtgacgttgg gcgcccttac gatagtgccg cggctggttg cccacccgac tgagtcgttt ggcctgcgga tcaccgatcc gagcggtgcg tcactggctt atagcggcga caccggcatt tgtgaccagc tcgtcgagct ggctcgcggc gtcgacgttt tcctctgcga ggcctcctgg acacactcgc ccaaacatcc acccgatcta cacctgtcgg gcaccgaagc cggtatggtt gccgcgcaag ccggcgttcg tgagctgctg ctgacgcata tcccgccgtg gacttcgcgt gaggacgtca tcagcgaggc caaggccgan ttcgacggcc cggtgcacgc ggtggtatgc gacgagacgt tcgaagtccg gcgagccggc taagtgagta agataggatc cggctgnnnn nnnnccccnn annnnnnnna nnnancgggt ngnannntng nncnanngnn tccaggangn naaaanntnt nnnnnnnncn cnnnncgnnt nna

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgtc gatgtattcc gcatcgttgt atcgggcatg gcacagtagt gtccgtgcgg ataaccgtgc tcggatgctc cggtagcgtc gtggggccgg attcgcctgc gtcggggtat ttgctccgag cgccgcacac accgccgttg gttatcgact tcggcggggg tgtgctcggc gcgctgcaac ggcacgcgga tcccgcgtcg gtgcatgtgc tgctgtcgca tctgcatgcg gaccattgtc tggacttgcc gggacttttt gtgtggcggc gttaccaccc gtcgcgtccc tctggcaagg cattgttgta cggccccagc gacacctggt cgcgattggg ggcggcgtcg tccccgtacg gtggggagat tgacgactgt tcggatatct tcgatgttca ccactgggcc gacagtgagc cagtgacgtt gggcgccctt acgatagtgc cgcggctggt tgcccacccg actgagtcgt ttggcctgcg gatcaccgat ccgagcggtg cgtcactggc ttatagcggc gacaccggca tttgtgacca gctcgtcgag ctggctcgcg gcgtcgacgt tttcctctgc gaggcctcct ggacacactc gcccaaacat ccacccgatc tacacctgtc gggcaccgaa gccggtatgg ttgccgcgca agccggcgtt cgtgagctgc tgctgacgca tatcccgccg tggacttcgc gtgaggacgt catcagcgag gccaaggccg agttcgacgg cccggtgcac gcggtggtat gcgacgagac gttcgaagtc cggcgagccg gctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat