MytuD.18689.a

Uncharacterized protein Rv1278/MT1315

CENTER ID: MytuD.18689.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18689.a.B2.GE37237 domain 1 1 471
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.1431
RefSeq: NP_215794.1
RefSeq: YP_006514653.1
TubercuList: Rv1278
MTB Network Portal: Rv1278
UniProt: P9WM41

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKLHRLALTN YRGIAHRDVE FPDHGVVVVC GANEIGKSSM VEALDLLLEY KDRSTKKEVK QVKPTNADVG SEVIAEISSG PYRFVYRKRF HKRCETELTV LAPRREQLTG DEAHERVRTM LAETVDTELW HAQRVLQAAS TAAVDLSGCD ALSRALDLAA GDDAALSGTE SLLIERIEAE YARYFTPTGR PTGEWSAAVS RLAAAEAAVA DCAAAVAEVD DGVRRHTELT EQVAELSQQL LAHQLRLEAA RVAAEKIAAI TDDAREAKLI ATAAAATSGA STAAHAGRLG LLTEIDTRTA AVVAAEAKAR QAADEQATAR AEAEACDAAL TEATQVLTAV RLRAESARRT LDQLADCEEA DRLAARLARI DDIEGDRDRV CAELSAVTLT EELLSRIERA AAAVDRGGAQ LASISAAVEF TAAVDIELGV GDQRVSLSAG QSWSVTATGP TEVKVPGVLT ARIVPGATAL DFQAKYAAAQ QELADALAAG EVADLAAARS ADLCRRELLS RRDQLTATLA GLCGDEQVDQ LRSRLEQLCA GQPAELDLVS TDTATARAEL DAVEAARIAA EKDCETRRQI AAGAARRLAE TSTRATVLQN AAAAESAELG AAMTRLACER ASVGDDELAA KAEADLRVLQ TAEQRVIDLA DELAATAPDA VAAELAEAAD AVELLRERHD EAIRALHEVG VELSVFGTQG RKGKLDAAET EREHAASHHA RVGRRARAAR LLRSVMARHR DTTRLRYVEP YRAELHRLGR PVFGPSFEVE VDTDLRIRSR TLDDRTVPYE CLSGGAKEQL GILARLAGAA LVAKEDAVPV LIDDALGFTD PERLAKMGEV FDTIGADGQV IVLTCSPTRY GGVKGAHRID LDAIQ
NT Sequence
gtgaagctgc accggctggc cctgaccaat taccgcggca tcgcacaccg tgacgtcgaa ttccccgatc atggagtggt ggtggtgtgc ggcgccaacg agatcggcaa gtcctccatg gtcgaggcgc tggacctgct gctcgagtac aaggaccgct cgacgaagaa ggaagtcaag caggtcaagc cgaccaacgc tgatgtcggc tccgaggtca ttgccgaaat cagcagcggc ccttatcgtt tcgtctaccg caagcgtttc cacaagcggt gcgagacgga gttgaccgtg ctggcaccgc gccgcgagca gctgaccggc gacgaagcgc acgagcgggt ccggacgatg ttggccgaaa cggtcgacac cgaactgtgg catgcccagc gggtgctgca ggccgcctcg acggccgcgg tggatctgtc tggctgcgac gcgctctcgc gtgcgctcga tctcgccgcc ggtgatgacg ccgcgctgtc gggcaccgag tcgctgctca tcgagcggat cgaggccgag tatgcgcgct acttcacccc gaccgggcgc cccaccggag aatggtccgc ggcggtctct aggctggcgg ccgccgaggc cgcggtggcc gactgcgcgg cggcggtagc cgaggtcgac gacggggttc gtcgccacac cgagctcacc gagcaggtgg ctgagctgtc gcagcaacta cttgctcacc agctgcggct cgaagctgcg cgagtcgccg ccgagaagat cgccgcaatc accgacgacg cccgcgaagc caagctgatc gctactgccg cggccgcgac cagcggcgct tccaccgccg cacacgccgg acggctgggc ctgctcaccg aaatcgacac gcgcactgcg gccgtcgttg ctgcggaggc aaaagcgcgg caggccgcag acgagcaggc gacggcgcgc gcggaggccg aggcctgcga tgccgcgctc acggaggcaa cccaggtatt gacggccgtc cgccttcgcg ccgagtcggc ccggcgcacc ctcgaccagc tcgccgactg cgaggaggcc gaccggttgg ccgcccggct ggccaggatc gacgacatcg agggtgatcg cgaccgggtc tgcgcggagc tgtccgcggt cacgctgacc gaggagctac tgagtcggat cgaacgtgct gcggcagccg tcgatcgcgg cggtgcacag ctggcgtcga tctccgcggc ggtggagttc accgccgccg tcgacatcga gctcggcgtc ggcgatcaac gggtgtcgct gtccgcgggc caaagctggt cggtcactgc caccggcccc accgaggtca aggttcccgg cgtcctgacc gcacggatcg tcccgggcgc gaccgcactc gactttcaag ccaaatatgc tgcagcacaa caggaattgg ctgatgcgct ggcggctgga gaggtcgctg acctagccgc cgcacgctcc gccgatctgt gccgacgcga actgctgagc cgccgcgatc agctgaccgc cactctggcc ggcctgtgtg gcgatgaaca ggtcgaccaa ctgcgttccc gcctggaaca gttgtgtgcc ggtcaaccgg ccgagctcga tctggtttcg acggataccg ctacggcccg cgctgaattg gatgcggtcg aggcggctcg aatcgccgcg gagaaggact gcgagacccg ccgtcagatc gctgctggcg ccgctcgccg gctcgcggag acatccacgc gggcaacggt tctacagaac gcagcggccg ccgaaagcgc cgagctcggt gcggccatga ctcggttggc ctgtgagcgg gcgtccgtgg gcgacgatga gctcgccgcc aaggccgagg ccgacctgcg ggtactgcag acggccgagc agcgagtgat cgacctggcc gacgagctcg cagctacggc gccggacgcg gtagccgccg agctggccga ggccgccgac gccgtcgagt tgctgcgcga acgtcacgac gaggccattc gcgcgttgca cgaggtcggc gtcgaactct cggtgttcgg cacccagggc cgcaagggca agcttgatgc cgccgaaacc gagcgtgagc acgccgccag ccaccacgcg cgggtcgggc gccgggcccg ggccgccagg ctgctccgct cggtgatggc acgccaccgc gacaccaccc ggctgcgcta cgtcgagcca taccgggcgg agctacatcg gctcggccgc ccagtgttcg ggccctcttt cgaggtcgag gtcgataccg atttgcgcat ccgcagccgc accctggacg acagaaccgt gccctacgag tgcttgtcgg gcggggccaa agaacagctt ggcatcctgg cgcgattggc cggcgcggcg ctggtcgcca aggaggacgc cgttccggtg ctgatcgacg acgcgctggg gttcaccgat ccggagcgac tagccaagat gggggaggtc tttgacacca tcggcgccga cggacaggtg atcgtgctga cgtgcagtcc cacccgatac ggcggtgtca aaggagcgca ccgcatcgat ctggacgcca tacagtga
Details for MytuD.18689.a.B2.GE37237
HARVESTED ON: 8/13/2013
SEQUENCED ON: 8/15/2013
EXPECTED MW: 51kDa
OBSERVED MW: 51kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 92
PERCENT COVERAGE: 71
Validated AA Sequence
MAHHHHHHMV KLHRLALTNY RGIAHRDVEF PDHGVVVVCG ANEIGKSSMV EALDLLLEYK DRSTKKEVKQ VKPTNADVGS EVIAEISSGP YRFVYRKRFH KRCETELTVL APRREQLTGD EAHERVRTML AETVDTELWH AQRVLQAAST AAVDLSGCDA LSRALDLAAG DDAALSGTES LLIERIEAEY ARYFTPTGRP TGEWSAAVSR LAAAEAAVAD CAAAVAEVDD GVRRHTELTE QVAELSQQLL AHQLRLEAAR VAAEKIAAIT DDAREAKLIA TAAAATSGAS TAAHAGRLGL LTEIDTRTAA VVAAXAKXXX XQTXRRXXXX XEXAXPRSXX XXX
Validated NT Sequence
tttgttnnnt ttaagaagga gatataccat ggctcaccac caccaccacc atatggtgaa gctgcaccgg ctggccctga ccaattaccg cggcatcgca caccgtgacg tcgaattccc cgatcatgga gtggtggtgg tgtgcggcgc caacgagatc ggcaagtcct ccatggtcga ggcgctggac ctgctgctcg agtacaagga ccgctcgacg aagaaggaag tcaagcaggt caagccgacc aacgctgatg tcggctccga ggtcattgcc gaaatcagca gcggccctta tcgtttcgtc taccgcaagc gtttccacaa gcggtgcgag acggagttga ccgtgctggc accgcgccgc gagcagctga ccggcgacga agcgcacgag cgggtccgga cgatgttggc cgaaacggtc gacaccgaac tgtggcatgc ccagcgggtg ctgcaggccg cctcgacggc cgcggtggat ctgtctggct gcgacgcgct ctcgcgtgcg ctcgatctcg ccgccggtga tgacgccgcg ctgtcgggca ccgagtcgct gctcatcgag cggatcgagg ccgagtatgc gcgctacttc accccgaccg ggcgccccac cggagaatgg tccgcggcgg tctctaggct ggcggccgcc gaggccgcgg tggccgactg cgcggcggcg gtagccgagg tcgacgacgg ggttcgtcgc cacaccgagc tcaccgagca ggtggctgag ctgtcgcagc aactacttgc tcaccagctg cggctcgaag ctgcgcgagt cgccgccgag aagatcgccg caatcaccga cgacgcccgc gaagccaagc tgatcgctac tgccgcggcc gcgaccagcg gcgcttccac cgccgcacac gccggacggc tgggcctgct caccgaaatc gacacgcgca ctgcggccgt cgtngctgcn nangcaaaan cgcngcnnnc gcagacnanc aggcgannnn cnnnnngagn cgagnntgcn atnccncgnt cannnnnnan ccnnnnntga ngnnnnncnn nnnnnnncna nngncngnnn nnnnccctcn acnnntcncn acngnnnnnn nnannnnncn nnnnnnngnn cnannnc
Expected Protein Sequence
MAHHHHHHMK LHRLALTNYR GIAHRDVEFP DHGVVVVCGA NEIGKSSMVE ALDLLLEYKD RSTKKEVKQV KPTNADVGSE VIAEISSGPY RFVYRKRFHK RCETELTVLA PRREQLTGDE AHERVRTMLA ETVDTELWHA QRVLQAASTA AVDLSGCDAL SRALDLAAGD DAALSGTESL LIERIEAEYA RYFTPTGRPT GEWSAAVSRL AAAEAAVADC AAAVAEVDDG VRRHTELTEQ VAELSQQLLA HQLRLEAARV AAEKIAAITD DAREAKLIAT AAAATSGAST AAHAGRLGLL TEIDTRTAAV VAAEAKARQA ADEQATARAE AEACDAALTE ATQVLTAVRL RAESARRTLD QLADCEEADR LAARLARIDD IEGDRDRVCA ELSAVTLTEE LLSRIERAAA AVDRGGAQLA SISAAVEFTA AVDIELGVGD QRVSLSAGQS WSVTATGPTE VKVPGVLTAR IVPGATALD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgaagc tgcaccggct ggccctgacc aattaccgcg gcatcgcaca ccgtgacgtc gaattccccg atcatggagt ggtggtggtg tgcggcgcca acgagatcgg caagtcctcc atggtcgagg cgctggacct gctgctcgag tacaaggacc gctcgacgaa gaaggaagtc aagcaggtca agccgaccaa cgctgatgtc ggctccgagg tcattgccga aatcagcagc ggcccttatc gtttcgtcta ccgcaagcgt ttccacaagc ggtgcgagac ggagttgacc gtgctggcac cgcgccgcga gcagctgacc ggcgacgaag cgcacgagcg ggtccggacg atgttggccg aaacggtcga caccgaactg tggcatgccc agcgggtgct gcaggccgcc tcgacggccg cggtggatct gtctggctgc gacgcgctct cgcgtgcgct cgatctcgcc gccggtgatg acgccgcgct gtcgggcacc gagtcgctgc tcatcgagcg gatcgaggcc gagtatgcgc gctacttcac cccgaccggg cgccccaccg gagaatggtc cgcggcggtc tctaggctgg cggccgccga ggccgcggtg gccgactgcg cggcggcggt agccgaggtc gacgacgggg ttcgtcgcca caccgagctc accgagcagg tggctgagct gtcgcagcaa ctacttgctc accagctgcg gctcgaagct gcgcgagtcg ccgccgagaa gatcgccgca atcaccgacg acgcccgcga agccaagctg atcgctactg ccgcggccgc gaccagcggc gcttccaccg ccgcacacgc cggacggctg ggcctgctca ccgaaatcga cacgcgcact gcggccgtcg ttgctgcgga ggcaaaagcg cggcaggccg cagacgagca ggcgacggcg cgcgcggagg ccgaggcctg cgatgccgcg ctcacggagg caacccaggt attgacggcc gtccgccttc gcgccgagtc ggcccggcgc accctcgacc agctcgccga ctgcgaggag gccgaccggt tggccgcccg gctggccagg atcgacgaca tcgagggtga tcgcgaccgg gtctgcgcgg agctgtccgc ggtcacgctg accgaggagc tactgagtcg gatcgaacgt gctgcggcag ccgtcgatcg cggcggtgca cagctggcgt cgatctccgc ggcggtggag ttcaccgccg ccgtcgacat cgagctcggc gtcggcgatc aacgggtgtc gctgtccgcg ggccaaagct ggtcggtcac tgccaccggc cccaccgagg tcaaggttcc cggcgtcctg accgcacgga tcgtcccggg cgcgaccgca ctcgactgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t