MytuD.18685.a

Uncharacterized protein Rv2030c/MT2089

CENTER ID: MytuD.18685.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18685.a.B1.GE37233 full length 1 681
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.2268
RefSeq: NP_216546.1
RefSeq: YP_006515442.1
TubercuList: Rv2030c
MTB Network Portal: Rv2030c
UniProt: P9WLM1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLMTAAADVT RRSPRRVFRD RREAGRVLAE LLAAYRDQPD VIVLGLARGG LPVAWEVAAA LHAPLDAFVV RKLGAPGHDE FAVGALASGG RVVVNDDVVR GLRITPQQLR DIAEREGREL LRRESAYRGE RPPTDITGKT VIVVDDGLAT GASMFAAVQA LRDAQPAQIV IAVPAAPEST CREFAGLVDD VVCATMPTPF LAVGESFWDF RQVTDEEVRR LLATPTAGPS LRRPAASTAA DVLRRVAIDA PGGVPTHEVL AELVGDARIV LIGESSHGTH EFYQARAAMT QWLIEEKGFG AVAAEADWPD AYRVNRYVRG LGEDTNADEA LSGFERFPAW MWRNTVVRDF VEWLRTRNQR YESGALRQAG FYGLDLYSLH RSIQEVISYL DKVDPRAAAR ARARYACFDH ACADDGQAYG FAAAFGAGPS CEREAVEQLV DVQRNALAYA RQDGLLAEDE LFYAQQNAQT VRDAEVYYRA MFSGRVTSWN LRDQHMAQTL GSLLTHLDRH LDAPPARIVV WAHNSHVGDA RATEVWADGQ LTLGQIVRER YGDESRSIGF STYTGTVTAA SEWGGIAQRK AVRPALHGSV EELFHQTADS FLVSARLSRD AEAPLDVVRL GRAIGVVYLP ATERQSHYLH VRPADQFDAM IHIDQTRALE PLEVTSRWIA GENPETYPTG L
NT Sequence
gtgctgatga ccgcagcggc tgatgtcacc cggcgctcgc cgcggcgcgt gttccgtgac cgccgcgagg ccggccgggt gctggcggaa ttactcgccg cctatcggga ccagccggac gtgattgtgc tcggcttggc ccggggtggc ctcccggtcg catgggaggt tgccgcggca ctgcatgccc cgctagacgc cttcgtcgtg cgcaaacttg gtgccccggg gcatgacgag ttcgccgttg gtgcactggc cagcggcggc cgcgtcgtgg tcaatgacga cgtcgtgcgg ggcctgcgga tcacaccgca gcaactgcgc gacatcgccg aacgtgaggg tcgggaactg cttcggcgcg agtccgccta ccgcggcgag cgcccgccca ccgatatcac cggcaagacg gtcattgtcg tcgatgacgg tttggccacc ggcgcaagca tgttcgcggc ggtacaggca ttgcgcgatg cgcaaccagc gcagatcgtg attgccgtgc cggcggcgcc ggagtccacg tgccgggagt tcgccggcct cgtcgacgac gttgtgtgcg cgaccatgcc gaccccgttc ctggccgtcg gtgagtcgtt ttgggacttc cggcaggtca ccgacgagga ggtccgccgg ctcctggcca ccccgaccgc tgggccgtcg ctgcgccggc ccgcggcgtc aacggcggcc gatgttctgc gcagagtcgc gatcgacgcc cccgggggtg ttccgacgca cgaggtgttg gcggagctgg tcggcgatgc acgaatcgtg ttgatcggcg aaagctcgca cggcacacac gagttctacc aggcccgggc cgccatgaca cagtggctga tcgaggagaa gggctttggt gcggtagccg ccgaggcgga ctggcccgac gcctaccggg tcaatcggta cgttcgcggc ctcggcgagg acaccaacgc tgacgaggcg cttagcggat tcgagcggtt tcccgcctgg atgtggcgca acaccgtggt ccgagatttt gtggaatggc tgcgcacacg caaccagcgc tacgagtcgg gcgcgctgcg gcaagccggc ttctacggtc tggatcttta cagcctgcat cggtcgatcc aagaggtgat cagctatctc gacaaggtcg acccgcgtgc ggcggcacgg gcgcgggccc ggtatgcgtg cttcgaccat gcctgcgccg atgacggtca ggcgtacgga ttcgcggccg cattcggcgc cggtccgtcg tgcgaacgtg aagccgtcga gcaactggtc gacgttcagc gcaatgccct ggcgtatgcg cgccaagacg ggctgcttgc cgaggacgaa ctgttctacg cccagcaaaa cgcgcagacg gtgcgcgacg cagaggtgta ttaccgggcc atgttcagtg gacgcgttac ctcgtggaac ctgcgcgacc agcacatggc gcagaccctt ggcagtttgc tgacgcattt ggaccgacac ctcgatgcgc cgccggcgcg aatagtggtg tgggctcata actcccacgt gggtgacgca cgcgctaccg aggtgtgggc cgacgggcag ctcaccctcg gccagatagt ccgtgagcga tacggtgacg agtcgcgcag catcggattc agcacgtaca cgggcaccgt caccgcggcc agcgaatggg gtggtatcgc ccaacgcaaa gcggttcggc cggcactgca cggcagtgtc gaggagctct tccaccagac tgcagacagt ttcctggtgt cagcgcggct aagccgcgac gccgaagccc cgctggacgt tgtccggttg ggacgtgcca tcggcgtcgt ttatctaccg gcaacggaac ggcaaagtca ctacttgcac gtgcggcccg ccgaccagtt cgacgccatg atccacatcg atcagacccg tgccctggaa cctctcgagg tgacgagccg gtggatcgcc ggcgagaacc cggaaaccta cccgaccggt ctgtag
Details for MytuD.18685.a.B1.GE37233
HARVESTED ON: 8/13/2013
SEQUENCED ON: 8/15/2013
EXPECTED MW: 76kDa
OBSERVED MW: 76kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 43
Validated AA Sequence
MAHHHHHHMV LMTAAADVTR RSPRRVFRDR REAGRVLAEL LAAYRDQPDV IVLGLARGGL PVAWEVAAAL HAPLDAFVVR KLGAPGHDEF AVGALASGGR VVVNDDVVRG LRITPQQLRD IAEREGRELL RRESAYRGER PPTDITGKTV IVVDDGLATG ASMFAAVQAL RDAQPAQIVI AVPAAPESTC REFAGLVDDV VCATMPTPFL AVGESFWDFR QVTDEEVRRL LATPTAGPSL RRPAASTAAD VLRRVAIDAP GGVPTHEVLA ELVGDARIVL IGESSHGTHE FYXXGXP
Validated NT Sequence
agannnnntt ntttacttta aganggagat ataccatggc tcaccaccac caccaccata tggtgctgat gaccgcagcg gctgatgtca cccggcgctc gccgcggcgc gtgttccgtg accgccgcga ggccggccgg gtgctggcgg aattactcgc cgcctatcgg gaccagccgg acgtgattgt gctcggcttg gcccggggtg gcctcccggt cgcatgggag gttgccgcgg cactgcatgc cccgctagac gccttcgtcg tgcgcaaact tggtgccccg gggcatgacg agttcgccgt tggtgcactg gccagcggcg gccgcgtcgt ggtcaatgac gacgtcgtgc ggggcctgcg gatcacaccg cagcaactgc gcgacatcgc cgaacgtgag ggtcgggaac tgcttcggcg cgagtccgcc taccgcggcg agcgcccgcc caccgatatc accggcaaga cggtcattgt cgtcgatgac ggtttggcca ccggcgcaag catgttcgcg gcggtacagg cattgcgcga tgcgcaacca gcgcagatcg tgattgccgt gccggcggcg ccggagtcca cgtgccggga gttcgccggc ctcgtcgacg acgttgtgtg cgcgaccatg ccgaccccgt tcctggccgt cggtgagtcg ttttgggact tccggcaggt caccgacgag gaggtccgcc ggctcctggc caccccgacc gctgggccgt cgctgcgccg gcccgcggcg tcaacggcgg ccgatgttct gcgcagagtc gcgatcgacg cccccggggg tgttccgacg cacgaggtgt tggcggagct ggtcggcgat gcacgaatcg tgttgatcgg cgaaagctcg cacggcacac acgagttcta ccnnnccggg ncgccntgac acagtggnnt gatcgaggan nagggctttg gtgcggtagc cgccnangcg gactggnccg acgcctacng ggncaatcng tacgttcgcg gcctngnnan gcnncgctga nnangnnctt nnnngnnnnn nnnnntnncc nncgnnnnnn nnnnnnnnnn atttnnnnnn nnnnnnnnnn nnnnannnnn ggnnnnnnnn nnnnnnnnnn nncnnnnngn nnnnnnnnnn nnngnnannn nang
Expected Protein Sequence
MAHHHHHHML MTAAADVTRR SPRRVFRDRR EAGRVLAELL AAYRDQPDVI VLGLARGGLP VAWEVAAALH APLDAFVVRK LGAPGHDEFA VGALASGGRV VVNDDVVRGL RITPQQLRDI AEREGRELLR RESAYRGERP PTDITGKTVI VVDDGLATGA SMFAAVQALR DAQPAQIVIA VPAAPESTCR EFAGLVDDVV CATMPTPFLA VGESFWDFRQ VTDEEVRRLL ATPTAGPSLR RPAASTAADV LRRVAIDAPG GVPTHEVLAE LVGDARIVLI GESSHGTHEF YQARAAMTQW LIEEKGFGAV AAEADWPDAY RVNRYVRGLG EDTNADEALS GFERFPAWMW RNTVVRDFVE WLRTRNQRYE SGALRQAGFY GLDLYSLHRS IQEVISYLDK VDPRAAARAR ARYACFDHAC ADDGQAYGFA AAFGAGPSCE REAVEQLVDV QRNALAYARQ DGLLAEDELF YAQQNAQTVR DAEVYYRAMF SGRVTSWNLR DQHMAQTLGS LLTHLDRHLD APPARIVVWA HNSHVGDARA TEVWADGQLT LGQIVRERYG DESRSIGFST YTGTVTAASE WGGIAQRKAV RPALHGSVEE LFHQTADSFL VSARLSRDAE APLDVVRLGR AIGVVYLPAT ERQSHYLHVR PADQFDAMIH IDQTRALEPL EVTSRWIAGE NPETYPTGL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgctga tgaccgcagc ggctgatgtc acccggcgct cgccgcggcg cgtgttccgt gaccgccgcg aggccggccg ggtgctggcg gaattactcg ccgcctatcg ggaccagccg gacgtgattg tgctcggctt ggcccggggt ggcctcccgg tcgcatggga ggttgccgcg gcactgcatg ccccgctaga cgccttcgtc gtgcgcaaac ttggtgcccc ggggcatgac gagttcgccg ttggtgcact ggccagcggc ggccgcgtcg tggtcaatga cgacgtcgtg cggggcctgc ggatcacacc gcagcaactg cgcgacatcg ccgaacgtga gggtcgggaa ctgcttcggc gcgagtccgc ctaccgcggc gagcgcccgc ccaccgatat caccggcaag acggtcattg tcgtcgatga cggtttggcc accggcgcaa gcatgttcgc ggcggtacag gcattgcgcg atgcgcaacc agcgcagatc gtgattgccg tgccggcggc gccggagtcc acgtgccggg agttcgccgg cctcgtcgac gacgttgtgt gcgcgaccat gccgaccccg ttcctggccg tcggtgagtc gttttgggac ttccggcagg tcaccgacga ggaggtccgc cggctcctgg ccaccccgac cgctgggccg tcgctgcgcc ggcccgcggc gtcaacggcg gccgatgttc tgcgcagagt cgcgatcgac gcccccgggg gtgttccgac gcacgaggtg ttggcggagc tggtcggcga tgcacgaatc gtgttgatcg gcgaaagctc gcacggcaca cacgagttct accaggcccg ggccgccatg acacagtggc tgatcgagga gaagggcttt ggtgcggtag ccgccgaggc ggactggccc gacgcctacc gggtcaatcg gtacgttcgc ggcctcggcg aggacaccaa cgctgacgag gcgcttagcg gattcgagcg gtttcccgcc tggatgtggc gcaacaccgt ggtccgagat tttgtggaat ggctgcgcac acgcaaccag cgctacgagt cgggcgcgct gcggcaagcc ggcttctacg gtctggatct ttacagcctg catcggtcga tccaagaggt gatcagctat ctcgacaagg tcgacccgcg tgcggcggca cgggcgcggg cccggtatgc gtgcttcgac catgcctgcg ccgatgacgg tcaggcgtac ggattcgcgg ccgcattcgg cgccggtccg tcgtgcgaac gtgaagccgt cgagcaactg gtcgacgttc agcgcaatgc cctggcgtat gcgcgccaag acgggctgct tgccgaggac gaactgttct acgcccagca aaacgcgcag acggtgcgcg acgcagaggt gtattaccgg gccatgttca gtggacgcgt tacctcgtgg aacctgcgcg accagcacat ggcgcagacc cttggcagtt tgctgacgca tttggaccga cacctcgatg cgccgccggc gcgaatagtg gtgtgggctc ataactccca cgtgggtgac gcacgcgcta ccgaggtgtg ggccgacggg cagctcaccc tcggccagat agtccgtgag cgatacggtg acgagtcgcg cagcatcgga ttcagcacgt acacgggcac cgtcaccgcg gccagcgaat ggggtggtat cgcccaacgc aaagcggttc ggccggcact gcacggcagt gtcgaggagc tcttccacca gactgcagac agtttcctgg tgtcagcgcg gctaagccgc gacgccgaag ccccgctgga cgttgtccgg ttgggacgtg ccatcggcgt cgtttatcta ccggcaacgg aacggcaaag tcactacttg cacgtgcggc ccgccgacca gttcgacgcc atgatccaca tcgatcagac ccgtgccctg gaacctctcg aggtgacgag ccggtggatc gccggcgaga acccggaaac ctacccgacc ggtctgtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t