MytuD.18684.a

Pup deamidase/depupylase (Dop); Pup--protein ligase (PafA)

CENTER ID: MytuD.18684.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18684.a.B1.GE37232 full length 1 505
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.2359
RefSeq: NP_216628.1
RefSeq: YP_006515528.1
TubercuList: Rv2112c
MTB Network Portal: Rv2112c
UniProt: P9WNU9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQRIIGTEVE YGISSPSDPT ANPILTSTQA VLAYAAAAGI QRAKRTRWDY EVESPLRDAR GFDLSRSAGP PPVVDADEVG AANMILTNGA RLYVDHAHPE YSAPECTDPL DAVIWDKAGE RVMEAAARHV ASVPGAAKLQ LYKNNVDGKG ASYGSHENYL MSRQTPFSAI ITGLTPFLVS RQVVTGSGRV GIGPSGDEPG FQLSQRSDYI EVEVGLETTL KRGIINTRDE PHADADRYRR LHVIIGDANL AETSTYLKLG TTALVLDLIE EGPAHAIDLT DLALARPVHA VHAISRDPSL RATVALADGR ELTGLALQRI YLDRVAKLVD SRDPDPRAAD IVETWAHVLD QLERDPMDCA ELLDWPAKLR LLDGFRQREN LSWSAPRLHL VDLQYSDVRL DKGLYNRLVA RGSMKRLVTE HQVLSAVENP PTDTRAYFRG ECLRRFGADI AAASWDSVIF DLGGDSLVRI PTLEPLRGSK AHVGALLDSV DSAVELVEQL TAEPR
NT Sequence
atgcagcgga ttatcggaac ggaggtcgag tacggcattt cctcgccgtc ggacccgacc gccaacccga tcctcacctc gacgcaggcg gtgctggcat acgccgccgc cgccggcatt cagcgtgcca aacgcacccg ttgggactac gaggtggaat cgccgctgcg cgacgcccgg ggcttcgatt tgagtcgctc ggccgggccg ccgccggtgg tcgacgccga cgaggtcggc gcggccaaca tgatcctgac caacggggcg cggctgtatg tcgaccacgc gcacccggaa tactccgcgc ccgaatgcac cgacccgctg gacgcagtga tctgggacaa ggcgggcgaa cgcgtgatgg aggccgctgc ccgccatgtc gccagcgtgc ccggggccgc gaaactgcag ctgtacaaga acaacgtcga cggcaaggga gcctcctacg ggtcgcacga gaactacctg atgtcgcggc agacaccgtt ctcggcgatc atcaccgggc tgaccccctt tctggtatcc cggcaggtgg tgaccggctc gggccgggtc ggcatcgggc cctcgggtga tgagcccggc ttccagctat cccagcgttc ggactacatc gaggtcgagg tagggctgga aacaacgctc aagcgcggca tcatcaacac ccgcgacgaa ccgcacgccg acgccgacag gtaccgccgg ctgcacgtca tcatcggcga cgccaacctt gccgagacgt cgacctatct gaagttgggt accacggcgc tggtgctcga cctgatcgaa gaaggaccag cccacgcaat agatctgacc gacctggcgc tggcccgccc ggtacatgcg gtgcacgcaa tctcccgcga tccgtcgctg cgagcgaccg ttgcgctggc cgacggccgg gaactgaccg gtcttgcgct gcaacggatc tacctggacc gagtggctaa gttggtggat agccgcgacc cggacccgcg ggcggccgac atcgtggaaa cctgggcaca cgtgctggat cagctcgagc gtgacccgat ggattgcgcg gagctgctgg actggccggc caaactgcgg ctgctcgacg gtttccggca gcgggagaac ctgagctggt cggcgccccg gctgcacctc gtcgacctgc agtactccga tgtccggctg gacaagggcc tgtacaaccg gctggtcgcg cgcggctcga tgaagcgttt agtcaccgaa caccaggtgc tgagtgcggt ggagaacccg ccgaccgaca cccgcgcgta tttccgcggc gaatgcctgc gccggttcgg ggctgatatc gccgcggcta gctgggactc ggtgatcttc gacctgggcg gcgactcgct ggttcgcatc ccgacgctgg agccgttgcg gggtagtaag gcgcatgttg gtgcgttgct ggattcggtg gacagtgccg tggagctggt agagcaactg accgctgagc ctcgctaa
Details for MytuD.18684.a.B1.GE37232
HARVESTED ON: 8/13/2013
SEQUENCED ON: 8/15/2013
EXPECTED MW: 56kDa
OBSERVED MW: 56kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 81
PERCENT COVERAGE: 75
Validated AA Sequence
MAHHHHHHMQ RIIGTEVEYG ISSPSDPTAN PILTSTQAVL AYAAAAGIQR AKRTRWDYEV ESPLRDARGF DLSRSAGPPP VVDADEVGAA NMILTNGARL YVDHAHPEYS APECTDPLDA VIWDKAGERV MEAAARHVAS VPGAAKLQLY KNNVDGKGAS YGSHENYLMS RQTPFSAIIT GLTPFLVSRQ VVTGSGRVGI GPSGDEPGFQ LSQRSDYIEV EVGLETTLKR GIINTRDEPH ADADRYRRLH VIIGDANLAE TSTYLKLGTT ALVLDLIEEX PAHAIDLTDL ALARXVHAVX AISRDPSLXA TVALXXGXXL XXXXCNXSTW XXXXXXXXXX XXGXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXX
Validated NT Sequence
ttttgtttac tttaagangg agatatacca tggctcacca ccaccaccac catatgcagc ggattatcgg aacggaggtc gagtacggca tttcctcgcc gtcggacccg accgccaacc cgatcctcac ctcgacgcag gcggtgctgg catacgccgc cgccgccggc attcagcgtg ccaaacgcac ccgttgggac tacgaggtgg aatcgccgct gcgcgacgcc cggggcttcg atttgagtcg ctcggccggg ccgccgccgg tggtcgacgc cgacgaggtc ggcgcggcca acatgatcct gaccaacggg gcgcggctgt atgtcgacca cgcgcacccg gaatactccg cgcccgaatg caccgacccg ctggacgcag tgatctggga caaggcgggc gaacgcgtga tggaggccgc tgcccgccat gtcgccagcg tgcccggggc cgcgaaactg cagctgtaca agaacaacgt cgacggcaag ggagcctcct acgggtcgca cgagaactac ctgatgtcgc ggcagacacc gttctcggcg atcatcaccg ggctgacccc ctttctggta tcccggcagg tggtgaccgg ctcgggccgg gtcggcatcg ggccctcggg tgatgagccc ggcttccagc tatcccagcg ttcggactac atcgaggtcg aggtagggct ggaaacaacg ctcaagcgcg gcatcatcaa cacccgcgac gaaccgcacg ccgacgccga caggtaccgc cggctgcacg tcatcatcgg cgacgccaac cttgccgaga cgtcgaccta tctgaagttg ggtaccacgg cgctggtgct cgacctgatc gaagaannac cagcccacgc aatagatctg accgacctgg cgctggcccg ccnggtacat gcngtgcang caatctcccg cgatccgtcg ctgcnagcga ccgtngcnct ggncganggc nnggnnctga nnntcntgng ctgcaacngn tctacctgga ncganngnta nnnnngnnan nnnnancnnn ncncnggnnn nnnnntcnnn nancnnnnnn nnnnnntgnn nanntngnnc gnnancnnat gnnnnnnnnn ntncnnnnnn nnnnnannnn nnnnnnnnnn nnnnnnnnng nnnnnnnnnn nnnnnnnnan nnnnannnnn nnc
Expected Protein Sequence
MAHHHHHHMQ RIIGTEVEYG ISSPSDPTAN PILTSTQAVL AYAAAAGIQR AKRTRWDYEV ESPLRDARGF DLSRSAGPPP VVDADEVGAA NMILTNGARL YVDHAHPEYS APECTDPLDA VIWDKAGERV MEAAARHVAS VPGAAKLQLY KNNVDGKGAS YGSHENYLMS RQTPFSAIIT GLTPFLVSRQ VVTGSGRVGI GPSGDEPGFQ LSQRSDYIEV EVGLETTLKR GIINTRDEPH ADADRYRRLH VIIGDANLAE TSTYLKLGTT ALVLDLIEEG PAHAIDLTDL ALARPVHAVH AISRDPSLRA TVALADGREL TGLALQRIYL DRVAKLVDSR DPDPRAADIV ETWAHVLDQL ERDPMDCAEL LDWPAKLRLL DGFRQRENLS WSAPRLHLVD LQYSDVRLDK GLYNRLVARG SMKRLVTEHQ VLSAVENPPT DTRAYFRGEC LRRFGADIAA ASWDSVIFDL GGDSLVRIPT LEPLRGSKAH VGALLDSVDS AVELVEQLTA EPR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcagc ggattatcgg aacggaggtc gagtacggca tttcctcgcc gtcggacccg accgccaacc cgatcctcac ctcgacgcag gcggtgctgg catacgccgc cgccgccggc attcagcgtg ccaaacgcac ccgttgggac tacgaggtgg aatcgccgct gcgcgacgcc cggggcttcg atttgagtcg ctcggccggg ccgccgccgg tggtcgacgc cgacgaggtc ggcgcggcca acatgatcct gaccaacggg gcgcggctgt atgtcgacca cgcgcacccg gaatactccg cgcccgaatg caccgacccg ctggacgcag tgatctggga caaggcgggc gaacgcgtga tggaggccgc tgcccgccat gtcgccagcg tgcccggggc cgcgaaactg cagctgtaca agaacaacgt cgacggcaag ggagcctcct acgggtcgca cgagaactac ctgatgtcgc ggcagacacc gttctcggcg atcatcaccg ggctgacccc ctttctggta tcccggcagg tggtgaccgg ctcgggccgg gtcggcatcg ggccctcggg tgatgagccc ggcttccagc tatcccagcg ttcggactac atcgaggtcg aggtagggct ggaaacaacg ctcaagcgcg gcatcatcaa cacccgcgac gaaccgcacg ccgacgccga caggtaccgc cggctgcacg tcatcatcgg cgacgccaac cttgccgaga cgtcgaccta tctgaagttg ggtaccacgg cgctggtgct cgacctgatc gaagaaggac cagcccacgc aatagatctg accgacctgg cgctggcccg cccggtacat gcggtgcacg caatctcccg cgatccgtcg ctgcgagcga ccgttgcgct ggccgacggc cgggaactga ccggtcttgc gctgcaacgg atctacctgg accgagtggc taagttggtg gatagccgcg acccggaccc gcgggcggcc gacatcgtgg aaacctgggc acacgtgctg gatcagctcg agcgtgaccc gatggattgc gcggagctgc tggactggcc ggccaaactg cggctgctcg acggtttccg gcagcgggag aacctgagct ggtcggcgcc ccggctgcac ctcgtcgacc tgcagtactc cgatgtccgg ctggacaagg gcctgtacaa ccggctggtc gcgcgcggct cgatgaagcg tttagtcacc gaacaccagg tgctgagtgc ggtggagaac ccgccgaccg acacccgcgc gtatttccgc ggcgaatgcc tgcgccggtt cggggctgat atcgccgcgg ctagctggga ctcggtgatc ttcgacctgg gcggcgactc gctggttcgc atcccgacgc tggagccgtt gcggggtagt aaggcgcatg ttggtgcgtt gctggattcg gtggacagtg ccgtggagct ggtagagcaa ctgaccgctg agcctcgctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat