MytuD.18682.a

PP2C-family Ser/Thr phosphatase (EC 3.1.3.16) (Mycobacterial Ser/Thr phosphatase) (Mstp) (Possible serine/threonine phosphatase Ppp)

CENTER ID: MytuD.18682.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18682.a.B2.GE37229 intracellular domain 1 240
MytuD.18682.a.B2.GE38117 intracellular domain 1 240
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.22
RefSeq: NP_214532.1
RefSeq: WP_010886062.1
RefSeq: YP_006513332.1
TubercuList: Rv0018c
MTB Network Portal: Rv0018c
UniProt: P9WHW5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MARVTLVLRY AARSDRGLVR ANNEDSVYAG ARLLALADGM GGHAAGEVAS QLVIAALAHL DDDEPGGDLL AKLDAAVRAG NSAIAAQVEM EPDLEGMGTT LTAILFAGNR LGLVHIGDSR GYLLRDGELT QITKDDTFVQ TLVDEGRITP EEAHSHPQRS LIMRALTGHE VEPTLTMREA RAGDRYLLCS DGLSDPVSDE TILEALQIPE VAESAHRLIE LALRGGGPDN VTVVVADVVD YDYGQTQPIL AGAVSGDDDQ LTLPNTAAGR ASAISQRKEI VKRVPPQADT FSRPRWSGRR LAFVVALVTV LMTAGLLIGR AIIRSNYYVA DYAGSVSIMR GIQGSLLGMS LHQPYLMGCL SPRNELSQIS YGQSGGPLDC HLMKLEDLRP PERAQVRAGL PAGTLDDAIG QLRELAANSL LPPCPAPRAT SPPGRPAPPT TSETTEPNVT SSPASPSPTT SAPAPTGTTP AIPTSASPAA PASPPTPWPV TSSPTMAALP PPPPQPGIDC RAAA
NT Sequence
gtggcgcgcg tgaccctggt cctgcgatac gcggcgcgca gcgatcgcgg cttggtacgc gccaacaacg aagactcggt ctacgctggg gcacggctat tggccctggc cgacggcatg ggtgggcatg cggccggcga ggtggcgtcc cagttggtga ttgccgcatt ggcccatctc gatgacgacg agcccggtgg cgatctgctg gccaagctgg atgccgcggt gcgcgccggc aactcggcta tcgcagcgca agtcgagatg gagcccgatc tcgaaggcat gggtaccacg ctcaccgcaa tcctgttcgc gggcaaccgg ctcggcctgg tgcatatcgg tgactcgcgc ggttacctgc tgcgcgacgg tgagctgacg cagatcacca aggacgacac gtttgtccaa acgctggtcg acgaaggccg gatcaccccg gaggaggcgc acagccaccc gcaacgctcg ttgatcatgc gggcgttgac cggccatgag gtcgaaccga cgctgaccat gcgagaagcc cgcgccggtg atcgttacct gctgtgctcg gacgggttgt ccgatccggt tagcgatgaa actatcctcg aggccctgca gatccccgag gttgccgaga gcgctcaccg cctcattgaa ctggcgctgc gcggcggcgg ccccgacaac gtcactgtcg tcgtcgccga cgtcgtcgac tacgactacg gccagaccca accgattctg gccggggcgg tctcaggcga cgacgaccaa ctgaccctgc ccaacaccgc cgccggccgg gcctctgcca tcagccagcg caaggagatc gttaaacgcg ttccgccaca ggccgataca ttcagtcggc cacggtggtc gggccgacgg ctagcattcg ttgtcgcact ggtgaccgtg ctgatgactg cgggcctgct cattggtcgc gcgatcatcc gcagcaacta ctacgtagcg gactacgccg gcagcgtgtc catcatgcgg gggattcaag ggtcgctact gggcatgtcc ctgcaccagc cttacctgat gggctgcctc agcccgcgta acgagctgtc gcagatcagc tacggacagt ctgggggccc tctcgactgc catctgatga aactggagga tctgcgaccg ccggagcgcg cacaggttcg ggccggtctc ccggccggca ctctcgatga cgccatcggg cagttgcgcg aactggcggc caactccctg ctgccgcctt gcccggcgcc gcgtgccacg tccccgcccg ggcgcccggc cccacccacc accagcgaga caaccgaacc aaacgtcacc tcctcgccag cctctccatc acccaccacc tccgcgccgg cccccaccgg aactactcct gccatcccca cgagtgcctc cccggcagcg cccgcgtcgc cgccgacgcc ttggcccgtc accagctcgc cgacgatggc cgcacttccg ccacccccgc ctcagccggg catcgactgc cgggcggcgg catga
Details for MytuD.18682.a.B2.GE37229
HARVESTED ON: 8/13/2013
SEQUENCED ON: 8/15/2013
EXPECTED MW: 26kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAPHHHHHMV ARVTLVLRYA ARSDRGLVRA NNEDSVYAGA RLLALADGMG GHAAGEVASQ LVIAALAHLD DDEPGGDLLA KLDAAVRAGN SAIAAQVEME PDLEGMGTTL TAILFAGNRL GLVHIGDSRG YLLRDGELTQ ITKDDTFVQT LVDEGRITPE EAHSHPQRSL IMRALTGHEV EPTLTMREAR AGDRYLLCSD GLSDPVSDET ILEALQIPEV AESAHRLIEL ALRGGGPDNV TVVVADVVD
Validated NT Sequence
cagccggatc ctatcttact cacttagtcg acgacgtcgg cgacgacgac agtgacgttg tcggggccgc cgccgcgcag cgccagttca atgaggcggt gagcgctctc ggcaacctcg gggatctgca gggcctcgag gatagtttca tcgctaaccg gatcggacaa cccgtccgag cacagcaggt aacgatcacc ggcgcgggct tctcgcatgg tcagcgtcgg ttcgacctca tggccggtca acgcccgcat gatcaacgag cgttgcgggt ggctgtgcgc ctcctccggg gtgatccggc cttcgtcgac cagcgtttgg acaaacgtgt cgtccttggt gatctgcgtc agctcaccgt cgcgcagcag gtaaccgcgc gagtcaccga tatgcaccag gccgagccgg ttgcccgcga acaggattgc ggtgagcgtg gtacccatgc cttcgagatc gggctccatc tcgacttgcg ctgcgatagc cgagttgccg gcgcgcaccg cggcatccag cttggccagc agatcgccac cgggctcgtc gtcatcgaga tgggccaatg cggcaatcac caactgggac gccacctcgc cggccgcatg cccacccatg ccgtcggcca gggccaatag ccgtgcccca gcgtagaccg agtcttcgtt gttggcgcgt accaagccgc gatcgctgcg cgccgcgtat cgcaggacca gggtcacgcg cgccaccata tggtggtggt ggtggggagc cat
Expected Protein Sequence
MAHHHHHHMA RVTLVLRYAA RSDRGLVRAN NEDSVYAGAR LLALADGMGG HAAGEVASQL VIAALAHLDD DEPGGDLLAK LDAAVRAGNS AIAAQVEMEP DLEGMGTTLT AILFAGNRLG LVHIGDSRGY LLRDGELTQI TKDDTFVQTL VDEGRITPEE AHSHPQRSLI MRALTGHEVE PTLTMREARA GDRYLLCSDG LSDPVSDETI LEALQIPEVA ESAHRLIELA LRGGGPDNVT VVVADVVD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtggcgc gcgtgaccct ggtcctgcga tacgcggcgc gcagcgatcg cggcttggta cgcgccaaca acgaagactc ggtctacgct ggggcacggc tattggccct ggccgacggc atgggtgggc atgcggccgg cgaggtggcg tcccagttgg tgattgccgc attggcccat ctcgatgacg acgagcccgg tggcgatctg ctggccaagc tggatgccgc ggtgcgcgcc ggcaactcgg ctatcgcagc gcaagtcgag atggagcccg atctcgaagg catgggtacc acgctcaccg caatcctgtt cgcgggcaac cggctcggcc tggtgcatat cggtgactcg cgcggttacc tgctgcgcga cggtgagctg acgcagatca ccaaggacga cacgtttgtc caaacgctgg tcgacgaagg ccggatcacc ccggaggagg cgcacagcca cccgcaacgc tcgttgatca tgcgggcgtt gaccggccat gaggtcgaac cgacgctgac catgcgagaa gcccgcgccg gtgatcgtta cctgctgtgc tcggacgggt tgtccgatcc ggttagcgat gaaactatcc tcgaggccct gcagatcccc gaggttgccg agagcgctca ccgcctcatt gaactggcgc tgcgcggcgg cggccccgac aacgtcactg tcgtcgtcgc cgacgtcgtc gactgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for MytuD.18682.a.B2.GE38117
HARVESTED ON: 3/27/2014
SEQUENCED ON: 5/23/2014
EXPECTED MW: 26kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAPHHHHHMV ARVTLVLRYA ARSDRGLVRA NNEDSVYAGA RLLALADGMG GHAAGEVASQ LVIAALAHLD DDEPGGDLLA KLDAAVRAGN SAIAAQVEME PDLEGMGTTL TAILFAGNRL GLVHIGDSRG YLLRDGELTQ ITKDDTFVQT LVDEGRITPE EAHSHPQRSL IMRALTGHEV EPTLTMREAR AGDRYLLCSD GLSDPVSDET ILEALQIPEV AESAHRLIEL ALRGGGPDNV TVVVADVVD
Validated NT Sequence
cagccggatc ctatcttact cacttagtcg acgacgtcgg cgacgacgac agtgacgttg tcggggccgc cgccgcgcag cgccagttca atgaggcggt gagcgctctc ggcaacctcg gggatctgca gggcctcgag gatagtttca tcgctaaccg gatcggacaa cccgtccgag cacagcaggt aacgatcacc ggcgcgggct tctcgcatgg tcagcgtcgg ttcgacctca tggccggtca acgcccgcat gatcaacgag cgttgcgggt ggctgtgcgc ctcctccggg gtgatccggc cttcgtcgac cagcgtttgg acaaacgtgt cgtccttggt gatctgcgtc agctcaccgt cgcgcagcag gtaaccgcgc gagtcaccga tatgcaccag gccgagccgg ttgcccgcga acaggattgc ggtgagcgtg gtacccatgc cttcgagatc gggctccatc tcgacttgcg ctgcgatagc cgagttgccg gcgcgcaccg cggcatccag cttggccagc agatcgccac cgggctcgtc gtcatcgaga tgggccaatg cggcaatcac caactgggac gccacctcgc cggccgcatg cccacccatg ccgtcggcca gggccaatag ccgtgcccca gcgtagaccg agtcttcgtt gttggcgcgt accaagccgc gatcgctgcg cgccgcgtat cgcaggacca gggtcacgcg cgccaccata tggtggtggt ggtggggagc cat
Expected Protein Sequence
MAHHHHHHMA RVTLVLRYAA RSDRGLVRAN NEDSVYAGAR LLALADGMGG HAAGEVASQL VIAALAHLDD DEPGGDLLAK LDAAVRAGNS AIAAQVEMEP DLEGMGTTLT AILFAGNRLG LVHIGDSRGY LLRDGELTQI TKDDTFVQTL VDEGRITPEE AHSHPQRSLI MRALTGHEVE PTLTMREARA GDRYLLCSDG LSDPVSDETI LEALQIPEVA ESAHRLIELA LRGGGPDNVT VVVADVVD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtggcgc gcgtgaccct ggtcctgcga tacgcggcgc gcagcgatcg cggcttggta cgcgccaaca acgaagactc ggtctacgct ggggcacggc tattggccct ggccgacggc atgggtgggc atgcggccgg cgaggtggcg tcccagttgg tgattgccgc attggcccat ctcgatgacg acgagcccgg tggcgatctg ctggccaagc tggatgccgc ggtgcgcgcc ggcaactcgg ctatcgcagc gcaagtcgag atggagcccg atctcgaagg catgggtacc acgctcaccg caatcctgtt cgcgggcaac cggctcggcc tggtgcatat cggtgactcg cgcggttacc tgctgcgcga cggtgagctg acgcagatca ccaaggacga cacgtttgtc caaacgctgg tcgacgaagg ccggatcacc ccggaggagg cgcacagcca cccgcaacgc tcgttgatca tgcgggcgtt gaccggccat gaggtcgaac cgacgctgac catgcgagaa gcccgcgccg gtgatcgtta cctgctgtgc tcggacgggt tgtccgatcc ggttagcgat gaaactatcc tcgaggccct gcagatcccc gaggttgccg agagcgctca ccgcctcatt gaactggcgc tgcgcggcgg cggccccgac aacgtcactg tcgtcgtcgc cgacgtcgtc gactgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat