SSGCID: Target Page

MytuD.18679.a

Nucleoside triphosphate pyrophosphohydrolase (NTP-PPase) (EC 3.6.1.8)

CENTER ID:	MytuD.18679.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.18679.a.B1.GE37227	full length		1	325

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.18679.a.B1.PW37414	full length		1	325
MytuD.18679.a.B1.PW37415	full length		1	325

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|83332.12.peg.1137
RefSeq:	NP_215537.1
RefSeq:	YP_006514382.1
TubercuList:	Rv1021
MTB Network Portal:	Rv1021
UniProt:	P96379

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIVVLVDPRR PTLVPVEAIE FLRGEVQYTE EMPVAVPWSL PAARSAHAGN DAPVLLSSDP NHPAVITRLA AGARLISAPD SQRGERLVDA VAMMDKLRTA GPWESEQTHD SLRRYLLEET YELLDAVRSG SVDQLREELG DLLLQVLFHA RIAEDASQSP FTIDDVADTL MRKLGNRAPG VLAGESISLE DQLAQWEAAK ASEKARKSVA DDVHTGQPAL ALAQKVIQRA QKAGLPAHLI PDEITSVSVS ADVDAENTLR TAVLDFIDRL RCAERAIAVA RRGSNVAEQL DVTPLGVITE QEWLAHWPTA VNDSRGGSKK RKGMR

NT Sequence

atgattgtcg tcctggtcga cccccggcgt ccgacactgg tgcctgttga agcgatcgag ttcctgcgcg gcgaggtgca atacaccgag gaaatgccgg tcgcggtgcc ctggtcgcta ccagcggctc gttcggcgca cgccggaaac gacgcgccgg tgttgctgtc gtctgacccc aaccatcctg ctgtcattac tcgactggcc gccggtgccc ggctgatctc ggcaccggat tctcagcgtg gcgaacgact cgtcgacgcc gtcgcgatga tggacaagct gcgcaccgcc ggaccgtggg aaagtgagca gactcacgac tcgctgcgca gatacctgct ggaggagacc tacgagctgt tggacgcggt ccgcagcggc agtgttgacc agctgcgcga agagcttggt gatctcttgc tgcaggtcct ctttcacgcc cggatcgctg aggatgcgtc gcaatcgccg ttcaccatcg acgacgtcgc cgacacactg atgcgaaagc tcggcaatcg ggcgccagga gtacttgcgg gcgaatcgat ttcgctcgaa gatcaactgg cgcaatggga ggcagccaag gcctcggaaa aggcgcgaaa gtcggtagcc gacgatgtcc atacgggcca gccggcatta gcgctggcgc agaaggttat tcagcgtgcc caaaaggctg ggctgcccgc tcacctgatc cccgatgaga tcacttctgt ttcggtttca gctgacgtag atgcggaaaa cacgctgcgc actgccgttt tggactttat tgacaggctg cgctgtgccg agcgggcaat tgccgtcgca cgccggggca gcaacgttgc cgagcagctc gatgtgacgc cgctgggtgt gatcaccgag caggagtggc tcgcgcattg gccaactgct gtcaacgatt cccgcggcgg gtccaagaaa cgtaaaggca tgcgataa

Details for MytuD.18679.a.B1.GE37227

HARVESTED ON:	8/13/2013
SEQUENCED ON:	8/15/2013
EXPECTED MW:	36kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMI VVLVDPRRPT LVPVEAIEFL RGEVQYTEEM PVAVPWSLPA ARSAHAGNDA PVLLSSDPNH PAVITRLAAG ARLISAPDSQ RGERLVDAVA MMDKLRTAGP WESEQTHDSL RRYLLEETYE LLDAVRSGSV DQLREELGDL LLQVLFHARI AEDASQSPFT IDDVADTLMR KLGNRAPGVL AGESISLEDQ LAQWEAAKAS EKARKSVADD VHTGQPALAL AQKVIQRAQK AGLPAHLIPD EITSVSVSAD VDAENTLRTA VLDFIDRLRC AERAIAVARR GSNVAEQLDV TPLGVITEQE WLAHWPTAVN DSRGGSKKRK GMR

Validated NT Sequence

tactcactta tcgcatgcct ttacgtttct tggacccgcc gcgggaatcg ttgacagcag ttggccaatg cgcgagccac tcctgctcgg tgatcacacc cagcggcgtc acatcgagct gctcggcaac gttgctgccc cggcgtgcga cggcaattgc ccgctcggca cagcgcagcc tgtcaataaa gtccaaaacg gcagtgcgca gcgtgttttc cgcatctacg tcagctgaaa ccgaaacaga agtgatctca tcggggatca ggtgagcggg cagcccagcc ttttgggcac gctgaataac cttctgcgcc agcgctaatg ccggctggcc cgtatggaca tcgtcggcta ccgactttcg cgccttttcc gaggccttgg ctgcctccca ttgcgccagt tgatcttcga gcgaaatcga ttcgcccgca agtactcctg gcgcccgatt gccgagcttt cgcatcagtg tgtcggcgac gtcgtcgatg gtgaacggcg attgcgacgc atcctcagcg atccgggcgt gaaagaggac ctgcagcaag agatcaccaa gctcttcgcg cagctggtca acactgccgc tgcggaccgc gtccaacagc tcgtaggtct cctccagcag gtatctgcgc agcgagtcgt gagtctgctc actttcccac ggtccggcgg tgcgcagctt gtccatcatc gcgacggcgt cgacgagtcg ttcgccacgc tgagaatccg gtgccgagat cagccgggca ccggcggcca gtcgagtaat gacagcagga tggttggggt cagacgacag caacaccggc gcgtcgtttc cggcgtgcgc cgaacgagcc gctggtagcg accagggcac cgcgaccggc atttcctcgg tgtattgcac ctcgccgcgc aggaactcga tcgcttcaac aggcaccagt gtcggacgcc gggggtcgac caggacgaca atcatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgattg tcgtcctggt cgacccccgg cgtccgacac tggtgcctgt tgaagcgatc gagttcctgc gcggcgaggt gcaatacacc gaggaaatgc cggtcgcggt gccctggtcg ctaccagcgg ctcgttcggc gcacgccgga aacgacgcgc cggtgttgct gtcgtctgac cccaaccatc ctgctgtcat tactcgactg gccgccggtg cccggctgat ctcggcaccg gattctcagc gtggcgaacg actcgtcgac gccgtcgcga tgatggacaa gctgcgcacc gccggaccgt gggaaagtga gcagactcac gactcgctgc gcagatacct gctggaggag acctacgagc tgttggacgc ggtccgcagc ggcagtgttg accagctgcg cgaagagctt ggtgatctct tgctgcaggt cctctttcac gcccggatcg ctgaggatgc gtcgcaatcg ccgttcacca tcgacgacgt cgccgacaca ctgatgcgaa agctcggcaa tcgggcgcca ggagtacttg cgggcgaatc gatttcgctc gaagatcaac tggcgcaatg ggaggcagcc aaggcctcgg aaaaggcgcg aaagtcggta gccgacgatg tccatacggg ccagccggca ttagcgctgg cgcagaaggt tattcagcgt gcccaaaagg ctgggctgcc cgctcacctg atccccgatg agatcacttc tgtttcggtt tcagctgacg tagatgcgga aaacacgctg cgcactgccg ttttggactt tattgacagg ctgcgctgtg ccgagcgggc aattgccgtc gcacgccggg gcagcaacgt tgccgagcag ctcgatgtga cgccgctggg tgtgatcacc gagcaggagt ggctcgcgca ttggccaact gctgtcaacg attcccgcgg cgggtccaag aaacgtaaag gcatgcgatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat

Details for MytuD.18679.a.B1.PW37414

PURIFICATION DATe:	12/16/2013
CONCENTRATION:	14.1mg/ml
OBSERVED MW:	36kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATTG TCGTCCTGGT CGACCCCCGG CGTCCGACAC TGGTGCCTGT TGAAGCGATC GAGTTCCTGC GCGGCGAGGT GCAATACACC GAGGAAATGC CGGTCGCGGT GCCCTGGTCG CTACCAGCGG CTCGTTCGGC GCACGCCGGA AACGACGCGC CGGTGTTGCT GTCGTCTGAC CCCAACCATC CTGCTGTCAT TACTCGACTG GCCGCCGGTG CCCGGCTGAT CTCGGCACCG GATTCTCAGC GTGGCGAACG ACTCGTCGAC GCCGTCGCGA TGATGGACAA GCTGCGCACC GCCGGACCGT GGGAAAGTGA GCAGACTCAC GACTCGCTGC GCAGATACCT GCTGGAGGAG ACCTACGAGC TGTTGGACGC GGTCCGCAGC GGCAGTGTTG ACCAGCTGCG CGAAGAGCTT GGTGATCTCT TGCTGCAGGT CCTCTTTCAC GCCCGGATCG CTGAGGATGC GTCGCAATCG CCGTTCACCA TCGACGACGT CGCCGACACA CTGATGCGAA AGCTCGGCAA TCGGGCGCCA GGAGTACTTG CGGGCGAATC GATTTCGCTC GAAGATCAAC TGGCGCAATG GGAGGCAGCC AAGGCCTCGG AAAAGGCGCG AAAGTCGGTA GCCGACGATG TCCATACGGG CCAGCCGGCA TTAGCGCTGG CGCAGAAGGT TATTCAGCGT GCCCAAAAGG CTGGGCTGCC CGCTCACCTG ATCCCCGATG AGATCACTTC TGTTTCGGTT TCAGCTGACG TAGATGCGGA AAACACGCTG CGCACTGCCG TTTTGGACTT TATTGACAGG CTGCGCTGTG CCGAGCGGGC AATTGCCGTC GCACGCCGGG GCAGCAACGT TGCCGAGCAG CTCGATGTGA CGCCGCTGGG TGTGATCACC GAGCAGGAGT GGCTCGCGCA TTGGCCAACT GCTGTCAACG ATTCCCGCGG CGGGTCCAAG AAACGTAAAG GCATGCGATG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT

Details for MytuD.18679.a.B1.PW37415

PURIFICATION DATe:	12/16/2013
CONCENTRATION:	34.7mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)