MytuD.18678.a
Putative 2-dehydropantoate 2-reductase
| CENTER ID: | MytuD.18678.a |
| ORGANISM: | Mycobacterium tuberculosis H37Rv |
| ASSOCIATED DISEASE: | Tuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MytuD.18678.a.B1.GE36981 | full length | 1 | 295 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MytuD.18678.a.B1.PW37391 | full length | 1 | 295 |
Structures
4OL9
DEPOSITED: 1/23/2014
DETERMINATION: XRay
CLONE: MytuD.18678.a.B1.GE36981
PROTEIN: MytuD.18678.a.B1.PW37391
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|83332.12.peg.2881 |
| RefSeq: | NP_217089.3 |
| TubercuList: | Rv2573 |
| MTB Network Portal: | Rv2573 |
| UniProt: | P9WIL1 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MATGIALVGP GAVGTTVAAL LHKAGYSPLL CGHTPRAGIE LRRDGADPIV VPGPVHTSPR EVAGPVDVLI LAVKATQNDA ARPWLTRLCD ERTVVAVLQN GVEQVEQVQP HCPSSAVVPA IVWCSAETQP QGWVRLRGEA ALVVPTGPAA EQFAGLLRGA GATVDCDPDF TTAAWRKLLV NALAGFMVLS GRRSAMFRRD DVAALSRRYV AECLAVARAE GARLDDDVVD EVVRLVRSAP QDMGTSMLAD RAAHRPLEWD LRNGVIVRKA RAHGLATPIS DVLVPLLAAA SDGPG
NT Sequence
attgcaacag gcattgccct agtcggaccc ggtgccgtcg gaacgacggt cgccgcgctg ttgcacaagg ccgggtattc gccgctgttg tgcggccaca ctccgcgcgc cgggatcgag ctccggcgag acggcgcaga ccccatcgtg gtgcccggtc cggtgcacac cagtcctcgg gaggttgccg gcccggtcga tgtgctgatc ctggcggtca aggccactca gaacgacgcc gcacgtccct ggctgacccg cctgtgcgac gagcgcaccg tggtggccgt gctgcaaaac ggtgtcgaac aggtcgagca ggtccagccg cattgtccgt cctcggccgt ggttcccgcg atcgtgtggt gttcggccga gacccagccg caagggtggg tgcgcttgcg cggtgaagcc gcactggtcg ttcccaccgg gcccgcggcc gagcagttcg ccgggctgct gcgcggtgcc ggcgccacgg tggactgcga ccccgacttc accacggcgg cctggcgcaa actactggtc aacgcgctgg cgggatttat ggtgctgtcc ggacggcggt cggcaatgtt ccgccgcgac gacgtcgcgg cattgtcgcg ccgctatgtc gccgaatgcc tggcggtggc gcgcgctgag ggtgcccgac tcgatgacga cgtcgtcgac gaagtggtcc gcctcgtccg gtcggccccg caggacatgg gcacctcgat gctggccgac cgggcagccc accggccact ggaatgggat ttgcgcaatg gggtgatcgt ccgcaaggcc cgcgcccacg gcctggccac cccgatcagc gacgtgctgg tgccgctgct ggcggctgcc agcgacggtc ccggatag
Details for MytuD.18678.a.B1.GE36981
| HARVESTED ON: | 5/21/2013 |
| SEQUENCED ON: | 5/23/2013 |
| EXPECTED MW: | 32kDa |
| OBSERVED MW: | 34kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMI ATGIALVGPG AVGTTVAALL HKAGYSPLLC GHTPRAGIEL RRDGADPIVV PGPVHTSPRE VAGPVDVLIL AVKATQNDAA RPWLTRLCDE RTVVAVLQNG VEQVEQVQPH CPSSAVVPAI VWCSAETQPQ GWVRLRGEAA LVVPTGPAAE QFAGLLRGAG ATVDCDPDFT TAAWRKLLVN ALAGFMVLSG RRSAMFRRDD VAALSRRYVA ECLAVARAEG ARLDDDVVDE VVRLVRSAPQ DMGTSMLADR AAHRPLEWDL RNGVIVRKAR AHGLATPISD VLVPLLAAAS DGPG
Validated NT Sequence
tcttactcac ttatccggga ccgtcgctgg cagccgccag cagcggcacc agcacgtcgc tgatcggggt ggccaggccg tgggcgcggg ccttgcggac gatcacccca ttgcgcaaat cccattccag tggccggtgg gctgcccggt cggccagcat cgaggtgccc atgtcctgcg gggccgaccg gacgaggcgg accacttcgt cgacgacgtc gtcatcgagt cgggcaccct cagcgcgcgc caccgccagg cattcggcga catagcggcg cgacaatgcc gcgacgtcgt cgcggcggaa cattgccgac cgccgtccgg acagcaccat aaatcccgcc agcgcgttga ccagtagttt gcgccaggcc gccgtggtga agtcggggtc gcagtccacc gtggcgccgg caccgcgcag cagcccggcg aactgctcgg ccgcgggccc ggtgggaacg accagtgcgg cttcaccgcg caagcgcacc cacccttgcg gctgggtctc ggccgaacac cacacgatcg cgggaaccac ggccgaggac ggacaatgcg gctggacctg ctcgacctgt tcgacaccgt tttgcagcac ggccaccacg gtgcgctcgt cgcacaggcg ggtcagccag ggacgtgcgg cgtcgttctg agtggccttg accgccagga tcagcacatc gaccgggccg gcaacctccc gaggactggt gtgcaccgga ccgggcacca cgatggggtc tgcgccgtct cgccggagct cgatcccggc gcgcggagtg tggccgcaca acagcggcga atacccggcc ttgtgcaaca gcgcggcgac cgtcgttccg acggcaccgg gtccgactag ggcaatgcct gttgcaatca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMA TGIALVGPGA VGTTVAALLH KAGYSPLLCG HTPRAGIELR RDGADPIVVP GPVHTSPREV AGPVDVLILA VKATQNDAAR PWLTRLCDER TVVAVLQNGV EQVEQVQPHC PSSAVVPAIV WCSAETQPQG WVRLRGEAAL VVPTGPAAEQ FAGLLRGAGA TVDCDPDFTT AAWRKLLVNA LAGFMVLSGR RSAMFRRDDV AALSRRYVAE CLAVARAEGA RLDDDVVDEV VRLVRSAPQD MGTSMLADRA AHRPLEWDLR NGVIVRKARA HGLATPISDV LVPLLAAASD GPG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catattgcaa caggcattgc cctagtcgga cccggtgccg tcggaacgac ggtcgccgcg ctgttgcaca aggccgggta ttcgccgctg ttgtgcggcc acactccgcg cgccgggatc gagctccggc gagacggcgc agaccccatc gtggtgcccg gtccggtgca caccagtcct cgggaggttg ccggcccggt cgatgtgctg atcctggcgg tcaaggccac tcagaacgac gccgcacgtc cctggctgac ccgcctgtgc gacgagcgca ccgtggtggc cgtgctgcaa aacggtgtcg aacaggtcga gcaggtccag ccgcattgtc cgtcctcggc cgtggttccc gcgatcgtgt ggtgttcggc cgagacccag ccgcaagggt gggtgcgctt gcgcggtgaa gccgcactgg tcgttcccac cgggcccgcg gccgagcagt tcgccgggct gctgcgcggt gccggcgcca cggtggactg cgaccccgac ttcaccacgg cggcctggcg caaactactg gtcaacgcgc tggcgggatt tatggtgctg tccggacggc ggtcggcaat gttccgccgc gacgacgtcg cggcattgtc gcgccgctat gtcgccgaat gcctggcggt ggcgcgcgct gagggtgccc gactcgatga cgacgtcgtc gacgaagtgg tccgcctcgt ccggtcggcc ccgcaggaca tgggcacctc gatgctggcc gaccgggcag cccaccggcc actggaatgg gatttgcgca atggggtgat cgtccgcaag gcccgcgccc acggcctggc caccccgatc agcgacgtgc tggtgccgct gctggcggct gccagcgacg gtcccggatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for MytuD.18678.a.B1.PW37391
| PURIFICATION DATe: | 11/6/2013 |
| CONCENTRATION: | 14.6mg/ml |
| OBSERVED MW: | 31kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMI ATGIALVGPG AVGTTVAALL HKAGYSPLLC GHTPRAGIEL RRDGADPIVV PGPVHTSPRE VAGPVDVLIL AVKATQNDAA RPWLTRLCDE RTVVAVLQNG VEQVEQVQPH CPSSAVVPAI VWCSAETQPQ GWVRLRGEAA LVVPTGPAAE QFAGLLRGAG ATVDCDPDFT TAAWRKLLVN ALAGFMVLSG RRSAMFRRDD VAALSRRYVA ECLAVARAEG ARLDDDVVDE VVRLVRSAPQ DMGTSMLADR AAHRPLEWDL RNGVIVRKAR AHGLATPISD VLVPLLAAAS DGPG
Validated NT Sequence
tcttactcac ttatccggga ccgtcgctgg cagccgccag cagcggcacc agcacgtcgc tgatcggggt ggccaggccg tgggcgcggg ccttgcggac gatcacccca ttgcgcaaat cccattccag tggccggtgg gctgcccggt cggccagcat cgaggtgccc atgtcctgcg gggccgaccg gacgaggcgg accacttcgt cgacgacgtc gtcatcgagt cgggcaccct cagcgcgcgc caccgccagg cattcggcga catagcggcg cgacaatgcc gcgacgtcgt cgcggcggaa cattgccgac cgccgtccgg acagcaccat aaatcccgcc agcgcgttga ccagtagttt gcgccaggcc gccgtggtga agtcggggtc gcagtccacc gtggcgccgg caccgcgcag cagcccggcg aactgctcgg ccgcgggccc ggtgggaacg accagtgcgg cttcaccgcg caagcgcacc cacccttgcg gctgggtctc ggccgaacac cacacgatcg cgggaaccac ggccgaggac ggacaatgcg gctggacctg ctcgacctgt tcgacaccgt tttgcagcac ggccaccacg gtgcgctcgt cgcacaggcg ggtcagccag ggacgtgcgg cgtcgttctg agtggccttg accgccagga tcagcacatc gaccgggccg gcaacctccc gaggactggt gtgcaccgga ccgggcacca cgatggggtc tgcgccgtct cgccggagct cgatcccggc gcgcggagtg tggccgcaca acagcggcga atacccggcc ttgtgcaaca gcgcggcgac cgtcgttccg acggcaccgg gtccgactag ggcaatgcct gttgcaatca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHMA TGIALVGPGA VGTTVAALLH KAGYSPLLCG HTPRAGIELR RDGADPIVVP GPVHTSPREV AGPVDVLILA VKATQNDAAR PWLTRLCDER TVVAVLQNGV EQVEQVQPHC PSSAVVPAIV WCSAETQPQG WVRLRGEAAL VVPTGPAAEQ FAGLLRGAGA TVDCDPDFTT AAWRKLLVNA LAGFMVLSGR RSAMFRRDDV AALSRRYVAE CLAVARAEGA RLDDDVVDEV VRLVRSAPQD MGTSMLADRA AHRPLEWDLR NGVIVRKARA HGLATPISDV LVPLLAAASD GPG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATTGCAA CAGGCATTGC CCTAGTCGGA CCCGGTGCCG TCGGAACGAC GGTCGCCGCG CTGTTGCACA AGGCCGGGTA TTCGCCGCTG TTGTGCGGCC ACACTCCGCG CGCCGGGATC GAGCTCCGGC GAGACGGCGC AGACCCCATC GTGGTGCCCG GTCCGGTGCA CACCAGTCCT CGGGAGGTTG CCGGCCCGGT CGATGTGCTG ATCCTGGCGG TCAAGGCCAC TCAGAACGAC GCCGCACGTC CCTGGCTGAC CCGCCTGTGC GACGAGCGCA CCGTGGTGGC CGTGCTGCAA AACGGTGTCG AACAGGTCGA GCAGGTCCAG CCGCATTGTC CGTCCTCGGC CGTGGTTCCC GCGATCGTGT GGTGTTCGGC CGAGACCCAG CCGCAAGGGT GGGTGCGCTT GCGCGGTGAA GCCGCACTGG TCGTTCCCAC CGGGCCCGCG GCCGAGCAGT TCGCCGGGCT GCTGCGCGGT GCCGGCGCCA CGGTGGACTG CGACCCCGAC TTCACCACGG CGGCCTGGCG CAAACTACTG GTCAACGCGC TGGCGGGATT TATGGTGCTG TCCGGACGGC GGTCGGCAAT GTTCCGCCGC GACGACGTCG CGGCATTGTC GCGCCGCTAT GTCGCCGAAT GCCTGGCGGT GGCGCGCGCT GAGGGTGCCC GACTCGATGA CGACGTCGTC GACGAAGTGG TCCGCCTCGT CCGGTCGGCC CCGCAGGACA TGGGCACCTC GATGCTGGCC GACCGGGCAG CCCACCGGCC ACTGGAATGG GATTTGCGCA ATGGGGTGAT CGTCCGCAAG GCCCGCGCCC ACGGCCTGGC CACCCCGATC AGCGACGTGC TGGTGCCGCT GCTGGCGGCT GCCAGCGACG GTCCCGGATG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT