MytuD.18675.a

CRISPR type III-associated RAMP protein Csm5 (CRISPR type III-A/Mtube-associated RAMP protein Csm5)

CENTER ID: MytuD.18675.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18675.a.B1.GE37224 full length 1 375
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.3144
RefSeq: NP_217335.1
RefSeq: YP_006516268.1
TubercuList: Rv2819c
MTB Network Portal: Rv2819c
UniProt: P9WJF5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNTYLKPFEL TLRCLGPVFI GSGEKRTSKE YHVEGDRVYF PDMELLYADI PAHKRKSFEA FVMNTDGAQA TAPLKEWVEP NAVKLDPAKH RGYEVKIGSI EPRRASRGRG GRMTRKKLTL NEIHAFIKDP LGRPYVPGST VKGMLRSIYL QSLVHKRTAQ PVRVPGHQTR EHRQYGERFE RKELRKSGRP NTRPQDAVND LFQAIRVTDS PALRTSDLLI CQKMDMNVHG KPDGLPLFRE CLAPGTSISH RVVVDTSPTA RGGWREGERF LETLAETAAS VNQARYAEYR AMYPGVNAIV GPIVYLGGGA GYRSKTFVTD QDDMAKVLDA QFGKVVKHVD KTRELRVSPL VLKRTKIDNI CYEMGQCELS IRRAE
NT Sequence
atgaacacct acctgaagcc gttcgaactc acgctgcggt gcctggggcc ggtgtttatc ggatccggcg agaagcggac ctcgaaggag taccacgtgg agggcgaccg ggtctacttc ccggacatgg aacttcttta cgcagacatt ccggctcaca agaggaagtc tttcgaagcg ttcgtcatga acaccgatgg ggcacaggcg acggcgccac tcaaagagtg ggtagagcca aacgcggtca agctggatcc tgctaagcat cgaggttacg aggtgaagat cgggtcgatc gaaccgcgac gtgcatctcg tggtcgaggc gggcgcatga ctcgaaagaa gcttacgctc aacgagattc acgctttcat caaagaccct cttggaaggc cctacgtgcc gggttcgact gtcaagggaa tgcttcgcag catctacctg cagtcgcttg tgcataagcg gacggcccaa cctgttcgtg ttccgggaca ccagacgcgg gagcaccggc agtacggcga aaggtttgag cggaaggagt tgcgcaaatc ggggcgcccc aacacccgtc cgcaagacgc ggtcaacgac ctgtttcagg cgatcagggt caccgactca cctgcactga gaacaagcga tctgctgatc tgccagaaga tggacatgaa tgtccacggc aagcctgatg gcctgccgct cttccgggaa tgtttggcgc cgggaacctc aatctcgcac cgcgtggtgg tcgacaccag tcccaccgct cgcggcggct ggcgtgaggg cgagcggttc cttgaaacgc tggccgagac agccgcgtcc gtgaatcagg cgcgttacgc ggagtacaga gccatgtacc ctggcgtgaa cgcgatagtt ggcccaattg tctatctggg cggcggagcc ggctatcgga gcaagacctt tgtcaccgac caagacgaca tggcgaaggt gctcgacgcc cagttcggga aggtagtcaa gcacgtcgac aagacgcgcg aactacgcgt ctcaccactt gtcttgaagc gaaccaagat cgacaacata tgctacgaga tgggtcagtg cgagctgtcg atcaggagag ccgaatga
Details for MytuD.18675.a.B1.GE37224
HARVESTED ON: 8/13/2013
SEQUENCED ON: 8/15/2013
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMN TYLKPFELTL RCLGPVFIGS GEKRTSKEYH VEGDRVYFPD MELLYADIPA HKRKSFEAFV MNTDGAQATA PLKEWVEPNA VKLDPAKHRG YEVKIGSIEP RRASRGRGGR MTRKKLTLNE IHAFIKDPLG RPYVPGSTVK GMLRSIYLQS LVHKRTAQPV RVPGHQTREH RQYGERFERK ELRKSGRPNT RPQDAVNDLF QAIRVTDSPA LRTSDLLICQ KMDMNVHGKP DGLPLFRECL APGTSISHRV VVDTSPTARG GWREGERFLE TLAETAASVN QARYAEYRAM YPGVNAIVGP IVYLGGGAGY RSKTFVTDQD DMAKVLDAQF GKVVKHVDKT RELRVSPLVL KRTKIDNICY EMGQCELSIR RAE
Validated NT Sequence
tattcggctc tcctgatcga cagctcgcac tgacccatct cgtagcatat gttgtcgatc ttggttcgct tcaagacaag tggtgagacg cgtagttcgc gcgtcttgtc gacgtgcttg actaccttcc cgaactgggc gtcgagcacc ttcgccatgt cgtcttggtc ggtgacaaag gtcttgctcc gatagccggc tccgccgccc agatagacaa ttgggccaac tatcgcgttc acgccagggt acatggctct gtactccgcg taacgcgcct gattcacgga cgcggctgtc tcggccagcg tttcaaggaa ccgctcgccc tcacgccagc cgccgcgagc ggtgggactg gtgtcgacca ccacgcggtg cgagattgag gttcccggcg ccaaacattc ccggaagagc ggcaggccat caggcttgcc gtggacattc atgtccatct tctggcagat cagcagatcg cttgttctca gtgcaggtga gtcggtgacc ctgatcgcct gaaacaggtc gttgaccgcg tcttgcggac gggtgttggg gcgccccgat ttgcgcaact ccttccgctc aaacctttcg ccgtactgcc ggtgctcccg cgtctggtgt cccggaacac gaacaggttg ggccgtccgc ttatgcacaa gcgactgcag gtagatgctg cgaagcattc ccttgacagt cgaacccggc acgtagggcc ttccaagagg gtctttgatg aaagcgtgaa tctcgttgag cgtaagcttc tttcgagtca tgcgcccgcc tcgaccacga gatgcacgtc gcggttcgat cgacccgatc ttcacctcgt aacctcgatg cttagcagga tccagcttga ccgcgtttgg ctctacccac tctttgagtg gcgccgtcgc ctgtgcccca tcggtgttca tgacgaacgc ttcgaaagac ttcctcttgt gagccggaat gtctgcgtaa agaagttcca tgtccgggaa gtagacccgg tcgccctcca cgtggtactc cttcgaggtc cgcttctcgc cggatccgat aaacaccggc cccaggcacc gcagcgtgag ttcgaacggc ttcaggtagg tgttcatatg gtggtggtgg tggtgagcca tggtat
Expected Protein Sequence
MAHHHHHHMN TYLKPFELTL RCLGPVFIGS GEKRTSKEYH VEGDRVYFPD MELLYADIPA HKRKSFEAFV MNTDGAQATA PLKEWVEPNA VKLDPAKHRG YEVKIGSIEP RRASRGRGGR MTRKKLTLNE IHAFIKDPLG RPYVPGSTVK GMLRSIYLQS LVHKRTAQPV RVPGHQTREH RQYGERFERK ELRKSGRPNT RPQDAVNDLF QAIRVTDSPA LRTSDLLICQ KMDMNVHGKP DGLPLFRECL APGTSISHRV VVDTSPTARG GWREGERFLE TLAETAASVN QARYAEYRAM YPGVNAIVGP IVYLGGGAGY RSKTFVTDQD DMAKVLDAQF GKVVKHVDKT RELRVSPLVL KRTKIDNICY EMGQCELSIR RAE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaca cctacctgaa gccgttcgaa ctcacgctgc ggtgcctggg gccggtgttt atcggatccg gcgagaagcg gacctcgaag gagtaccacg tggagggcga ccgggtctac ttcccggaca tggaacttct ttacgcagac attccggctc acaagaggaa gtctttcgaa gcgttcgtca tgaacaccga tggggcacag gcgacggcgc cactcaaaga gtgggtagag ccaaacgcgg tcaagctgga tcctgctaag catcgaggtt acgaggtgaa gatcgggtcg atcgaaccgc gacgtgcatc tcgtggtcga ggcgggcgca tgactcgaaa gaagcttacg ctcaacgaga ttcacgcttt catcaaagac cctcttggaa ggccctacgt gccgggttcg actgtcaagg gaatgcttcg cagcatctac ctgcagtcgc ttgtgcataa gcggacggcc caacctgttc gtgttccggg acaccagacg cgggagcacc ggcagtacgg cgaaaggttt gagcggaagg agttgcgcaa atcggggcgc cccaacaccc gtccgcaaga cgcggtcaac gacctgtttc aggcgatcag ggtcaccgac tcacctgcac tgagaacaag cgatctgctg atctgccaga agatggacat gaatgtccac ggcaagcctg atggcctgcc gctcttccgg gaatgtttgg cgccgggaac ctcaatctcg caccgcgtgg tggtcgacac cagtcccacc gctcgcggcg gctggcgtga gggcgagcgg ttccttgaaa cgctggccga gacagccgcg tccgtgaatc aggcgcgtta cgcggagtac agagccatgt accctggcgt gaacgcgata gttggcccaa ttgtctatct gggcggcgga gccggctatc ggagcaagac ctttgtcacc gaccaagacg acatggcgaa ggtgctcgac gcccagttcg ggaaggtagt caagcacgtc gacaagacgc gcgaactacg cgtctcacca cttgtcttga agcgaaccaa gatcgacaac atatgctacg agatgggtca gtgcgagctg tcgatcagga gagccgaatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat