MytuD.18674.a

CONSERVED HYPOTHETICAL ALANINE RICH PROTEIN (Ribonuclease D, putative)

CENTER ID: MytuD.18674.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18674.a.B1.GE37223 full length 1 438
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.2991
RefSeq: NP_217197.1
RefSeq: YP_006516125.1
TubercuList: Rv2681
MTB Network Portal: Rv2681
UniProt: I6XF17

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MCPEPSHAGA AESEGTESEP TPLLRPAGGI PDLCVTVGEI AAAAELLDRG RGPFAVDAER ASGFRYSGRA YLIQIRRAEA GTVLIDPVSH GGDPLTVLAP VAEVLSTNEW ILHSADQDLP CLAEVGMRPP ALYDTELAGR LAGFDRVNLA AMVERLLGLG LTKGHGAADW SKRPLPSAWL NYAALDVELL IELRAAISRV LAEQGKTDWA AQEFEHLRSF ESRPPPAAAR QDRWRRTSGI HKVHDRRGLA AVRELWTARD RIAQRRDIAP RRILPDSAII DAAIADPKSV DDLVALPVFG GRNQRRSAAV WWAALAAARE SPDPPEIAEP ANGPPPPGRW VRRKPAAAAR LDAARAALTE VSQRVRVPTE NLVSPDLVRR LCWEWEDISQ SSPDPIAAVE AYLRTGQARA WQLELVVPIL TAALTGAPDA GAQGDDGS
NT Sequence
atgtgccccg agccgtctca cgcgggagct gctgagtccg aaggcacgga atcggaaccc acccccttgc tccggcccgc cggtgggata ccggatctgt gtgtgaccgt cggtgaaatc gccgctgccg cagaactact ggaccgcggg cgcggaccgt tcgcggtaga cgccgagcgg gcgtcgggtt tccgctactc cggccgcgcc tacctgattc agatccggcg ggccgaggcc ggcaccgtac tgatcgaccc ggtcagccac ggcggtgacc cgttgaccgt gctggcgccg gtcgccgagg tgctcagcac caacgagtgg atcctgcact ccgccgatca ggatctgccc tgtctcgccg aggtcggtat gcgaccgcca gcgctatacg acaccgagct tgccgggcgc ctggccgggt tcgatcgagt gaacctggcg gccatggtcg agcggttact tggactggga ttgaccaagg gccacggcgc ggccgactgg tccaagcgcc cgctaccctc ggcctggctg aactacgcgg cgttggacgt ggaactgctc atcgaactac gcgcggcgat ctcgcgggtg ctggccgagc aaggcaaaac cgattgggct gcgcaggaat tcgagcacct gcggtcgttc gaatcaaggc cacccccagc ggccgcccgg caggaccgct ggcgacgaac ctcgggtatc cacaaagtgc atgaccggcg ggggctggcc gcggtccgcg aattgtggac agcgcgtgac cgaatcgccc agcgccgcga catcgcgccc cgccggatct tgccggactc ggccattatc gatgccgcca tcgccgaccc aaagtcagtc gacgaccttg tcgcgttacc ggtgttcggc ggacgcaacc aacgtcgcag cgcggctgtg tggtgggcgg cactggcagc cgcacgcgaa agcccagatc cgccggagat cgccgaaccg gcaaacgggc cgccgccgcc ggggcggtgg gtcagacgga aaccggcagc cgccgcacgg ctggatgcgg cgcgcgcggc gctgacggag gtgtcgcaac gggtgcgggt accgaccgag aacctggtct cacctgatct ggtgcgacgg ctgtgttggg aatgggagga catctcgcag agttctccag acccgattgc cgctgtcgag gcgtacctgc gcaccggcca ggcacgggcc tggcagctcg aactagtggt ccccatcctg accgcggcgt tgacaggggc tccggacgcc ggcgcccagg gcgatgatgg ctcttag
Details for MytuD.18674.a.B1.GE37223
HARVESTED ON: 8/13/2013
SEQUENCED ON: 8/15/2013
EXPECTED MW: 48kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 73
PERCENT COVERAGE: 68
Validated AA Sequence
MRPPALYDTE LAGRLAGFDR VNLAAMVERL LGLGLTKGHG AADWSKRPLP SAWLNYAALD VELLIELRAA ISRVLAEQGK TDWAAQEFEH LRSFESRPPP AAARQDRWRR TSGIHKVHDR RGLAAVRELW TARDRIAQRR DIAPRRILPD SAIIDAXXAD PKSVDDXVAL PVXXXXNXXR XXXXXWXAXX XXXESPXXPE IXXXXXGXXX PXXXXRRKXX XAXRXXXXRX XLXXXXQXXX VXXEXXVSXD XXRXXXXXXE XIXXSSPXXX XAVEXYXXTG QARAXXLXXV VPILTXXLTG APDX
Validated NT Sequence
cggngtccgg agcccctgtc aannncncgg tcaggatggg gaccactann ncnagntgnc aggcccgtgc ctggccggtg nnnnngtacn cctcgacngc gnnnatnnnn tntggagaac tntgcnagat gtnctcncat tnnnnannnn nccntcgnnn nnnatcnngt ganaccangn tctcgnnnng nacccnnann ngttgcnacn nctnnnncag cnnnnnncgn gncncannnn nccgtgnggc ggntgnnnnt ttccgtctga nnnnnnnncn cggcngnnnn nnnccgnttg ncgnntnnnc gatctccggc gnatntgggc tttcnngtgn ggntgncnnt gccgnccanc acananncnc nctgcgacnn tggttgcnnn nnnnnaacac cggtaacgcg acanngtcgt cgactgactt tgggtcggcg annnnggcat cgataatggc cgagtccggc aagatccggc ggggcgcgat gtcgcggcgc tgggcgattc ggtcacgcgc tgtccacaat tcgcggaccg cggccagccc ccgccggtca tgcactttgt ggatacccga ggttcgtcgc cagcggtcct gccgggcggc cgctgggggt ggccttgatt cgaacgaccg caggtgctcg aattcctgcg cagcccaatc ggttttgcct tgctcggcca gcacccgcga gatcgccgcg cgtagttcga tgagcagttc cacgtccaac gccgcgtagt tcagccaggc cgagggtagc gggcgcttgg accagtcggc cgcgccgtgg cccttggtca atcccagtcc aagtaaccgc tcgaccatgg ccgccaggtt cactcgatcg aacccggcca ggcgcccggc aagctcggtg tcgtatagcg ctggcggtcg cataccgacc tcggcgagac agggcagatc ctgatcggcg gagtgcagga tccactcgtt ggtgctgagc acctcggcga ccggcgccag cacggtcaac gggtcaccgc cgtggctgac cgggtcgatc agtacggtgc cggcctcggc ccgccggatc tgaatcaggt aggcgcggcc ggagtagcgg aaacccgacg cccgctcggc gtctaccgcg aacggtccgc gcccgcggtc cagtagttct gcggcagcgg cgatttcacc gacggtcaca cacagatccg gtatcccacc ggcgggccgg agcaaggggg tgggttccga ttccgtgcct tcggactcag cagctcccgc gtgagacggc tc
Expected Protein Sequence
MAHHHHHHMC PEPSHAGAAE SEGTESEPTP LLRPAGGIPD LCVTVGEIAA AAELLDRGRG PFAVDAERAS GFRYSGRAYL IQIRRAEAGT VLIDPVSHGG DPLTVLAPVA EVLSTNEWIL HSADQDLPCL AEVGMRPPAL YDTELAGRLA GFDRVNLAAM VERLLGLGLT KGHGAADWSK RPLPSAWLNY AALDVELLIE LRAAISRVLA EQGKTDWAAQ EFEHLRSFES RPPPAAARQD RWRRTSGIHK VHDRRGLAAV RELWTARDRI AQRRDIAPRR ILPDSAIIDA AIADPKSVDD LVALPVFGGR NQRRSAAVWW AALAAARESP DPPEIAEPAN GPPPPGRWVR RKPAAAARLD AARAALTEVS QRVRVPTENL VSPDLVRRLC WEWEDISQSS PDPIAAVEAY LRTGQARAWQ LELVVPILTA ALTGAPDAGA QGDDGS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtgcc ccgagccgtc tcacgcggga gctgctgagt ccgaaggcac ggaatcggaa cccaccccct tgctccggcc cgccggtggg ataccggatc tgtgtgtgac cgtcggtgaa atcgccgctg ccgcagaact actggaccgc gggcgcggac cgttcgcggt agacgccgag cgggcgtcgg gtttccgcta ctccggccgc gcctacctga ttcagatccg gcgggccgag gccggcaccg tactgatcga cccggtcagc cacggcggtg acccgttgac cgtgctggcg ccggtcgccg aggtgctcag caccaacgag tggatcctgc actccgccga tcaggatctg ccctgtctcg ccgaggtcgg tatgcgaccg ccagcgctat acgacaccga gcttgccggg cgcctggccg ggttcgatcg agtgaacctg gcggccatgg tcgagcggtt acttggactg ggattgacca agggccacgg cgcggccgac tggtccaagc gcccgctacc ctcggcctgg ctgaactacg cggcgttgga cgtggaactg ctcatcgaac tacgcgcggc gatctcgcgg gtgctggccg agcaaggcaa aaccgattgg gctgcgcagg aattcgagca cctgcggtcg ttcgaatcaa ggccaccccc agcggccgcc cggcaggacc gctggcgacg aacctcgggt atccacaaag tgcatgaccg gcgggggctg gccgcggtcc gcgaattgtg gacagcgcgt gaccgaatcg cccagcgccg cgacatcgcg ccccgccgga tcttgccgga ctcggccatt atcgatgccg ccatcgccga cccaaagtca gtcgacgacc ttgtcgcgtt accggtgttc ggcggacgca accaacgtcg cagcgcggct gtgtggtggg cggcactggc agccgcacgc gaaagcccag atccgccgga gatcgccgaa ccggcaaacg ggccgccgcc gccggggcgg tgggtcagac ggaaaccggc agccgccgca cggctggatg cggcgcgcgc ggcgctgacg gaggtgtcgc aacgggtgcg ggtaccgacc gagaacctgg tctcacctga tctggtgcga cggctgtgtt gggaatggga ggacatctcg cagagttctc cagacccgat tgccgctgtc gaggcgtacc tgcgcaccgg ccaggcacgg gcctggcagc tcgaactagt ggtccccatc ctgaccgcgg cgttgacagg ggctccggac gccggcgccc agggcgatga tggctcttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at