MytuD.18669.a
Putative uncharacterized protein
| CENTER ID: | MytuD.18669.a |
| ORGANISM: | Mycobacterium tuberculosis H37Rv |
| ASSOCIATED DISEASE: | Tuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MytuD.18669.a.B1.GE37220 | full length | 1 | 345 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MytuD.18669.a.B1.PW37800 | full length | 1 | 345 |
Structures
4Q6U
DEPOSITED: 4/23/2014
DETERMINATION: XRay
CLONE: MytuD.18669.a.B1.GE37220
PROTEIN: MytuD.18669.a.B1.PD00311
Publications by SSGCID
Mycobacterium tuberculosis Rv3651 is a triple sensor-domain protein
Abendroth J, Edwards TE, Grundner C, Myler PJ, Phan IQ, Staker BL, Frando A
Protein Sci. - 2017
volume 27, issue 2, pages 568-572
PMID: 29119630; PMCID: PMC5775179
PDB:
4Q6U
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|83332.12.peg.4070 |
| RefSeq: | NP_218168.1 |
| RefSeq: | YP_006517138.1 |
| TubercuList: | Rv3651 |
| MTB Network Portal: | Rv3651 |
| UniProt: | I6YCP0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTHDWLLVET LGDEPAVVAR GRELKKLVPI TTFLRRSPYL AAVRTAIAET LQTGQSLTSI TPKHDRVIRT EPVIMTDGRM HGVQVWSGPT DAEPPDRPIP GPLKWDLTRG VATDTPESLT NSGKNPEVEI TYGRAFAEDL PARELNPNET QVLAMAVKAK PGKTLCSIWD LTDWQGTPIR IGFVARSALE PGPNGRDHLV ARAMNWRAET KAPAVPVDDL AQRILIGLAQ AGVHRALVDL KTWTLLKWLD QPCSFYDWRR SAADGPRLHP DDQHVIDAMT RDLANGSASH VLRLPGHDVD WVPVHVTVNR IELEPDTFAG LVALRLPTDE ELADAGLPKA TDVTT
NT Sequence
atgacccacg actggctgct cgtggagacg ctgggggacg aaccggccgt ggtagcacgg gggcgtgagc tgaagaagct cgtcccgatc accacgttcc tgcgtcgcag tccctatttg gcggcggtcc gcacagctat cgccgagacg ctgcagaccg gccaaagcct gaccagcatc actcccaagc acgatcgcgt catccgcacc gaacctgtaa taatgaccga cggccgcatg cacggcgtgc aggtgtggag tggccccaca gacgccgaac cgcccgaccg gccgatccca ggcccgctga agtgggacct gacccgtggt gtggccaccg acaccccgga gtcactgacc aacagcggca agaatcccga ggtcgagatc acctacggcc gagccttcgc cgaagacctg ccggcgcgcg agctcaatcc gaacgaaacc caggtgcttg ccatggcagt taaagccaag cccggcaaaa cactatgcag catttgggat ctcactgatt ggcaaggaac acccatccgg atcggcttcg tggcgcgaag cgctctggag ccgggaccaa acggccgcga tcacctggtc gcccgggcaa tgaattggcg tgctgagacc aaggcccctg cagtgcccgt cgacgacttg gctcagcgga tccttatcgg actggcgcag gccggagtcc accgggcact ggtcgatctc aaaacctgga ccctgctgaa atggctcgac caaccctgct ctttctacga ctggcggcgt agcgcggccg atgggcctcg tctacatccc gacgaccagc acgtgatcga cgccatgaca agagacctcg ccaacggatc ggccagtcat gtgctgcgct tgcctgggca cgacgtcgat tgggtgccgg tccatgtcac cgtcaaccgg atagagctcg aaccggatac cttcgctgga ctggtcgctc tgcgactgcc caccgacgaa gaacttgccg acgccggact gccgaaagcc accgacgtca ccacctga
Details for MytuD.18669.a.B1.GE37220
| HARVESTED ON: | 8/13/2013 |
| SEQUENCED ON: | 1/13/2014 |
| EXPECTED MW: | 39kDa |
| OBSERVED MW: | 39kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MTHDWLLVET LGDEPAVVAR GRELKKLVPI TTFLRRSPYL AAVRTAIAET LQTGQSLTSI TPKHDRVIRT EPVIMTDGRM HGVQVWSGPT DAEPPDRPIP GPLKWDLTRG VATDTPESLT NSGKNPEVEI TYGRAFAEDL PARELNPNET QVLAMAVKAK PGKTLCSIWD LTDWQGTPIR IGFVARSALE PGPNGRDHLV ARAMNWRAET KAPAVPVDDL AQRILIGLAQ AGVHRALVDL KTWTLLKWLD QPCSFYDWRR SAADGPRLHP DDQHVIDAMT RDLANGSASH VLRLPGHDVD WVPVHVTVNR IELEPDTFAG LVALRLPTDE ELADAGLPKA TDVTT
Validated NT Sequence
catatgaccc acgactggct gctcgtggag acgctggggg acgaaccggc cgtggtagca cgggggcgtg agctgaagaa gctcgtcccg atcaccacgt tcctgcgtcg cagtccctat ttggcggcgg tccgcacagc tatcgccgag acgctgcaga ccggccaaag cctgaccagc atcactccca agcacgatcg cgtcatccgc accgaacctg taataatgac cgacggccgc atgcacggcg tgcaggtgtg gagtggcccc acagacgccg aaccgcccga ccggccgatc ccaggcccgc tgaagtggga cctgacccgt ggtgtggcca ccgacacccc ggagtcactg accaacagcg gcaagaatcc cgaggtcgag atcacctacg gccgagcctt cgccgaagac ctgccggcgc gcgagctcaa tccgaacgaa acccaggtgc ttgccatggc agttaaagcc aagcccggca aaacactatg cagcatttgg gatctcactg attggcaagg aacacccatc cggatcggct tcgtggcgcg aagcgctctg gagccgggac caaacggccg cgatcacctg gtcgcccggg caatgaattg gcgtgctgag accaaggccc ctgcagtgcc cgtcgacgac ttggctcagc ggatccttat cggactggcg caggccggag tccaccgggc actggtcgat ctcaaaacct ggaccctgct gaaatggctc gaccaaccct gctctttcta cgactggcgg cgtagcgcgg ccgatgggcc tcgtctacat cccgacgacc agcacgtgat cgacgccatg acaagagacc tcgccaacgg atcggccagt catgtgctgc gcttgcctgg gcacgacgtc gattgggtgc cggtccatgt caccgtcaac cggatagagc tcgaaccgga taccttcgct ggactggtcg ctctgcgact gcccaccgac gaagaacttg ccgacgccgg actgccgaaa gccaccgacg tcaccaccta agtgagtaag at
Expected Protein Sequence
MAHHHHHHMT HDWLLVETLG DEPAVVARGR ELKKLVPITT FLRRSPYLAA VRTAIAETLQ TGQSLTSITP KHDRVIRTEP VIMTDGRMHG VQVWSGPTDA EPPDRPIPGP LKWDLTRGVA TDTPESLTNS GKNPEVEITY GRAFAEDLPA RELNPNETQV LAMAVKAKPG KTLCSIWDLT DWQGTPIRIG FVARSALEPG PNGRDHLVAR AMNWRAETKA PAVPVDDLAQ RILIGLAQAG VHRALVDLKT WTLLKWLDQP CSFYDWRRSA ADGPRLHPDD QHVIDAMTRD LANGSASHVL RLPGHDVDWV PVHVTVNRIE LEPDTFAGLV ALRLPTDEEL ADAGLPKATD VTT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccc acgactggct gctcgtggag acgctggggg acgaaccggc cgtggtagca cgggggcgtg agctgaagaa gctcgtcccg atcaccacgt tcctgcgtcg cagtccctat ttggcggcgg tccgcacagc tatcgccgag acgctgcaga ccggccaaag cctgaccagc atcactccca agcacgatcg cgtcatccgc accgaacctg taataatgac cgacggccgc atgcacggcg tgcaggtgtg gagtggcccc acagacgccg aaccgcccga ccggccgatc ccaggcccgc tgaagtggga cctgacccgt ggtgtggcca ccgacacccc ggagtcactg accaacagcg gcaagaatcc cgaggtcgag atcacctacg gccgagcctt cgccgaagac ctgccggcgc gcgagctcaa tccgaacgaa acccaggtgc ttgccatggc agttaaagcc aagcccggca aaacactatg cagcatttgg gatctcactg attggcaagg aacacccatc cggatcggct tcgtggcgcg aagcgctctg gagccgggac caaacggccg cgatcacctg gtcgcccggg caatgaattg gcgtgctgag accaaggccc ctgcagtgcc cgtcgacgac ttggctcagc ggatccttat cggactggcg caggccggag tccaccgggc actggtcgat ctcaaaacct ggaccctgct gaaatggctc gaccaaccct gctctttcta cgactggcgg cgtagcgcgg ccgatgggcc tcgtctacat cccgacgacc agcacgtgat cgacgccatg acaagagacc tcgccaacgg atcggccagt catgtgctgc gcttgcctgg gcacgacgtc gattgggtgc cggtccatgt caccgtcaac cggatagagc tcgaaccgga taccttcgct ggactggtcg ctctgcgact gcccaccgac gaagaacttg ccgacgccgg actgccgaaa gccaccgacg tcaccacctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for MytuD.18669.a.B1.PW37800
| PURIFICATION DATe: | 10/19/2015 |
| CONCENTRATION: | 17.5mg/ml |
| OBSERVED MW: | 42kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 10 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MTHDWLLVET LGDEPAVVAR GRELKKLVPI TTFLRRSPYL AAVRTAIAET LQTGQSLTSI TPKHDRVIRT EPVIMTDGRM HGVQVWSGPT DAEPPDRPIP GPLKWDLTRG VATDTPESLT NSGKNPEVEI TYGRAFAEDL PARELNPNET QVLAMAVKAK PGKTLCSIWD LTDWQGTPIR IGFVARSALE PGPNGRDHLV ARAMNWRAET KAPAVPVDDL AQRILIGLAQ AGVHRALVDL KTWTLLKWLD QPCSFYDWRR SAADGPRLHP DDQHVIDAMT RDLANGSASH VLRLPGHDVD WVPVHVTVNR IELEPDTFAG LVALRLPTDE ELADAGLPKA TDVTT
Validated NT Sequence
catatgaccc acgactggct gctcgtggag acgctggggg acgaaccggc cgtggtagca cgggggcgtg agctgaagaa gctcgtcccg atcaccacgt tcctgcgtcg cagtccctat ttggcggcgg tccgcacagc tatcgccgag acgctgcaga ccggccaaag cctgaccagc atcactccca agcacgatcg cgtcatccgc accgaacctg taataatgac cgacggccgc atgcacggcg tgcaggtgtg gagtggcccc acagacgccg aaccgcccga ccggccgatc ccaggcccgc tgaagtggga cctgacccgt ggtgtggcca ccgacacccc ggagtcactg accaacagcg gcaagaatcc cgaggtcgag atcacctacg gccgagcctt cgccgaagac ctgccggcgc gcgagctcaa tccgaacgaa acccaggtgc ttgccatggc agttaaagcc aagcccggca aaacactatg cagcatttgg gatctcactg attggcaagg aacacccatc cggatcggct tcgtggcgcg aagcgctctg gagccgggac caaacggccg cgatcacctg gtcgcccggg caatgaattg gcgtgctgag accaaggccc ctgcagtgcc cgtcgacgac ttggctcagc ggatccttat cggactggcg caggccggag tccaccgggc actggtcgat ctcaaaacct ggaccctgct gaaatggctc gaccaaccct gctctttcta cgactggcgg cgtagcgcgg ccgatgggcc tcgtctacat cccgacgacc agcacgtgat cgacgccatg acaagagacc tcgccaacgg atcggccagt catgtgctgc gcttgcctgg gcacgacgtc gattgggtgc cggtccatgt caccgtcaac cggatagagc tcgaaccgga taccttcgct ggactggtcg ctctgcgact gcccaccgac gaagaacttg ccgacgccgg actgccgaaa gccaccgacg tcaccaccta agtgagtaag at
Expressed Protein Sequence
MAHHHHHHMT HDWLLVETLG DEPAVVARGR ELKKLVPITT FLRRSPYLAA VRTAIAETLQ TGQSLTSITP KHDRVIRTEP VIMTDGRMHG VQVWSGPTDA EPPDRPIPGP LKWDLTRGVA TDTPESLTNS GKNPEVEITY GRAFAEDLPA RELNPNETQV LAMAVKAKPG KTLCSIWDLT DWQGTPIRIG FVARSALEPG PNGRDHLVAR AMNWRAETKA PAVPVDDLAQ RILIGLAQAG VHRALVDLKT WTLLKWLDQP CSFYDWRRSA ADGPRLHPDD QHVIDAMTRD LANGSASHVL RLPGHDVDWV PVHVTVNRIE LEPDTFAGLV ALRLPTDEEL ADAGLPKATD VTT
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACCC ACGACTGGCT GCTCGTGGAG ACGCTGGGGG ACGAACCGGC CGTGGTAGCA CGGGGGCGTG AGCTGAAGAA GCTCGTCCCG ATCACCACGT TCCTGCGTCG CAGTCCCTAT TTGGCGGCGG TCCGCACAGC TATCGCCGAG ACGCTGCAGA CCGGCCAAAG CCTGACCAGC ATCACTCCCA AGCACGATCG CGTCATCCGC ACCGAACCTG TAATAATGAC CGACGGCCGC ATGCACGGCG TGCAGGTGTG GAGTGGCCCC ACAGACGCCG AACCGCCCGA CCGGCCGATC CCAGGCCCGC TGAAGTGGGA CCTGACCCGT GGTGTGGCCA CCGACACCCC GGAGTCACTG ACCAACAGCG GCAAGAATCC CGAGGTCGAG ATCACCTACG GCCGAGCCTT CGCCGAAGAC CTGCCGGCGC GCGAGCTCAA TCCGAACGAA ACCCAGGTGC TTGCCATGGC AGTTAAAGCC AAGCCCGGCA AAACACTATG CAGCATTTGG GATCTCACTG ATTGGCAAGG AACACCCATC CGGATCGGCT TCGTGGCGCG AAGCGCTCTG GAGCCGGGAC CAAACGGCCG CGATCACCTG GTCGCCCGGG CAATGAATTG GCGTGCTGAG ACCAAGGCCC CTGCAGTGCC CGTCGACGAC TTGGCTCAGC GGATCCTTAT CGGACTGGCG CAGGCCGGAG TCCACCGGGC ACTGGTCGAT CTCAAAACCT GGACCCTGCT GAAATGGCTC GACCAACCCT GCTCTTTCTA CGACTGGCGG CGTAGCGCGG CCGATGGGCC TCGTCTACAT CCCGACGACC AGCACGTGAT CGACGCCATG ACAAGAGACC TCGCCAACGG ATCGGCCAGT CATGTGCTGC GCTTGCCTGG GCACGACGTC GATTGGGTGC CGGTCCATGT CACCGTCAAC CGGATAGAGC TCGAACCGGA TACCTTCGCT GGACTGGTCG CTCTGCGACT GCCCACCGAC GAAGAACTTG CCGACGCCGG ACTGCCGAAA GCCACCGACG TCACCACCTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT