MytuD.18667.a

Uncharacterized glycosyltransferase Rv1514c/MT1564 (EC 2.4.-.-)

CENTER ID: MytuD.18667.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18667.a.B1.GE37218 full length 1 262
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.1690
RefSeq: NP_216030.1
RefSeq: YP_006514901.1
TubercuList: Rv1514c
MTB Network Portal: Rv1514c
UniProt: P9WMX9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTSAPTVSVI TISFNDLDGL QRTVKSVRAQ RYRGRIEHIV IDGGSGDDVV AYLSGCEPGF AYWQSEPDGG RYDAMNQGIA HASGDLLWFL HSADRFSGPD VVAQAVEALS GKGPVSELWG FGMDRLVGLD RVRGPIPFSL RKFLAGKQVV PHQASFFGSS LVAKIGGYDL DFGIAADQEF ILRAALVCEP VTIRCVLCEF DTTGVGSHRE PSAVFGDLRR MGDLHRRYPF GGRRISHAYL RGREFYAYNS RFWENVFTRM SK
NT Sequence
gtgacgtctg ctccgaccgt ctcggtgata acgatctcgt tcaacgacct cgacgggttg cagcgcacgg tgaaaagtgt gcgggcgcaa cgctaccggg gacgcatcga gcacatcgta atcgacggtg gcagcggcga cgacgtggtg gcatacctgt ccgggtgtga accaggcttc gcgtattggc agtccgagcc cgacggcggg cggtacgacg cgatgaacca gggcatcgcg cacgcatcgg gtgatctgtt gtggttcttg cactccgccg atcgtttttc cgggcccgac gtggtagccc aggccgtgga ggcgctatcc ggcaagggac cggtgtccga attgtggggc ttcgggatgg atcgtctcgt cgggctcgat cgggtgcgcg gcccgatacc tttcagcctg cgcaaattcc tggccggcaa gcaggttgtt ccgcatcaag catcgttctt cggatcgtcg ctggtggcca agatcggtgg ctacgacctt gatttcggga tcgccgccga ccaggaattc atattgcggg ccgcgctggt atgcgagccg gtcacgattc ggtgtgtgct gtgcgagttc gacaccacgg gcgtcggctc gcaccgggaa ccaagcgcgg tcttcggtga tctgcgccgc atgggcgacc ttcatcgccg ctacccgttc gggggaaggc gaatatcaca tgcctaccta cgcggccggg agttctacgc ctacaacagt cgattctggg aaaacgtctt cacgcgaatg tcgaaatag
Details for MytuD.18667.a.B1.GE37218
HARVESTED ON: 8/13/2013
SEQUENCED ON: 8/15/2013
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV TSAPTVSVIT ISFNDLDGLQ RTVKSVRAQR YRGRIEHIVI DGGSGDDVVA YLSGCEPGFA YWQSEPDGGR YDAMNQGIAH ASGDLLWFLH SADRFSGPDV VAQAVEALSG KGPVSELWGF GMDRLVGLDR VRGPIPFSLR KFLAGKQVVP HQASFFGSSL VAKIGGYDLD FGIAADQEFI LRAALVCEPV TIRCVLCEFD TTGVGSHREP SAVFGDLRRM GDLHRRYPFG GRRISHAYLR GREFYAYNSR FWENVFTRMS K
Validated NT Sequence
atcttactca cttatttcga cattcgcgtg aagacgtttt cccagaatcg actgttgtag gcgtagaact cccggccgcg taggtaggca tgtgatattc gccttccccc gaacgggtag cggcgatgaa ggtcgcccat gcggcgcaga tcaccgaaga ccgcgcttgg ttcccggtgc gagccgacgc ccgtggtgtc gaactcgcac agcacacacc gaatcgtgac cggctcgcat accagcgcgg cccgcaatat gaattcctgg tcggcggcga tcccgaaatc aaggtcgtag ccaccgatct tggccaccag cgatgatccg aagaacgatg cttgatgcgg aacaacctgc ttgccggcca ggaatttgcg caggctgaaa ggtatcgggc cgcgcacccg atcgagcccg acgagacgat ccatcccgaa gccccacaat tcggacaccg gtcccttgcc ggatagcgcc tccacggcct gggctaccac gtcgggcccg gaaaaacgat cggcggagtg caagaaccac aacagatcac ccgatgcgtg cgcgatgccc tggttcatcg cgtcgtaccg cccgccgtcg ggctcggact gccaatacgc gaagcctggt tcacacccgg acaggtatgc caccacgtcg tcgccgctgc caccgtcgat tacgatgtgc tcgatgcgtc cccggtagcg ttgcgcccgc acacttttca ccgtgcgctg caacccgtcg aggtcgttga acgagatcgt tatcaccgag acggtcggag cagacgtcac catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMT SAPTVSVITI SFNDLDGLQR TVKSVRAQRY RGRIEHIVID GGSGDDVVAY LSGCEPGFAY WQSEPDGGRY DAMNQGIAHA SGDLLWFLHS ADRFSGPDVV AQAVEALSGK GPVSELWGFG MDRLVGLDRV RGPIPFSLRK FLAGKQVVPH QASFFGSSLV AKIGGYDLDF GIAADQEFIL RAALVCEPVT IRCVLCEFDT TGVGSHREPS AVFGDLRRMG DLHRRYPFGG RRISHAYLRG REFYAYNSRF WENVFTRMSK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgacgt ctgctccgac cgtctcggtg ataacgatct cgttcaacga cctcgacggg ttgcagcgca cggtgaaaag tgtgcgggcg caacgctacc ggggacgcat cgagcacatc gtaatcgacg gtggcagcgg cgacgacgtg gtggcatacc tgtccgggtg tgaaccaggc ttcgcgtatt ggcagtccga gcccgacggc gggcggtacg acgcgatgaa ccagggcatc gcgcacgcat cgggtgatct gttgtggttc ttgcactccg ccgatcgttt ttccgggccc gacgtggtag cccaggccgt ggaggcgcta tccggcaagg gaccggtgtc cgaattgtgg ggcttcggga tggatcgtct cgtcgggctc gatcgggtgc gcggcccgat acctttcagc ctgcgcaaat tcctggccgg caagcaggtt gttccgcatc aagcatcgtt cttcggatcg tcgctggtgg ccaagatcgg tggctacgac cttgatttcg ggatcgccgc cgaccaggaa ttcatattgc gggccgcgct ggtatgcgag ccggtcacga ttcggtgtgt gctgtgcgag ttcgacacca cgggcgtcgg ctcgcaccgg gaaccaagcg cggtcttcgg tgatctgcgc cgcatgggcg accttcatcg ccgctacccg ttcgggggaa ggcgaatatc acatgcctac ctacgcggcc gggagttcta cgcctacaac agtcgattct gggaaaacgt cttcacgcga atgtcgaaat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat