SSGCID: Target Page

MytuD.18530.a

Putative asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4)

CENTER ID:	MytuD.18530.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.18530.a.B1.GE36552	full length		1	652

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|83332.12.peg.2453
RefSeq:	NP_216717.1
RefSeq:	NP_336729.1
TubercuList:	Rv2201
MTB Network Portal:	Rv2201
UniProt:	P9WN33

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MCGLLAFVAA PAGAAGPEGA DAASAIARAS HLMRHRGPDE SGTWHAVDGA SGGVVFGFNR LSIIDIAHSH QPLRWGPPEA PDRYVLVFNG EIYNYLELRD ELRTQHGAVF ATDGDGEAIL AGYHHWGTEV LQRLRGMFAF ALWDTVTREL FCARDPFGIK PLFIATGAGG TAVASEKKCL LDLVELVGFD TEIDHRALQH YTVLQYVPEP ETLHRGVRRL ESGCFARIRA DQLAPVITRY FVPRFAASPI TNDNDQARYD EITAVLEDSV AKHMRADVTV GAFLSGGIDS TAIAALAIRH NPRLITFTTG FEREGFSEID VAVASAEAIG ARHIAKVVSA DEFVAALPEI VWYLDEPVAD PALVPLFFVA REARKHVKVV LSGEGADELF GGYTIYREPL SLRPFDYLPK PLRRSMGKVS KPLPEGMRGK SLLHRGSLTL EERYYGNARS FSGAQLREVL PGFRPDWTHT DVTAPVYAES AGWDPVARMQ HIDLFTWLRG DILVKADKIT MANSLELRVP FLDPEVFAVA SRLPAGAKIT RTTTKYALRR ALEPIVPAHV LHRPKLGFPV PIRHWLRAGE LLEWAYATVG SSQAGHLVDI AAVYRMLDEH RCGSSDHSRR LWTMLIFMLW HAIFVEHSVV PQISEPQYPV QL

NT Sequence

gtgtgtggac tgctggcctt cgtcgcggcc ccggccggtg ctgcggggcc cgaaggtgcc gacgctgcca gcgccatcgc ccgcgcatcg catttgatgc gccaccgcgg gcccgatgaa tcgggcacct ggcacgccgt cgatggcgcc tccggaggcg tcgtgttcgg gttcaaccga ctgtccatca tcgacatcgc gcactcgcat cagccgctgc ggtgggggcc gccggaggct ccggaccgct acgtgctggt gttcaacggc gagatctaca actacttgga gctgcgtgac gagctgcgca cccagcacgg cgctgtgttc gccaccgacg gcgacggtga ggcgatcctc gccggctatc accactgggg caccgaggtg ctgcagcggt tgcgcggcat gttcgcattc gcgctgtggg acaccgtcac ccgcgaattg ttctgcgcgc gagatccgtt cggcatcaag ccgttgttta tcgccaccgg agccggcggc acggcggtgg ccagtgagaa gaaatgcctg ctggacctcg tcgagttggt ggggttcgac accgagatcg accatcgggc gttgcagcac tacaccgtcc tgcagtacgt gccggaaccc gagacactgc accgtggggt acgtcggctg gaatcaggct gcttcgcccg gatccgtgcc gaccagctcg cgccggtgat cacccgttat ttcgtgccgc gatttgcggc cagtccgatc accaacgaca acgaccaggc ccgctatgac gagatcacgg cagtgcttga ggactcggtg gccaagcata tgcgcgccga tgtcaccgtc ggcgcgtttc tgtccggggg tatcgactcc acggccatcg cggcgctggc catccggcac aatccgcggc tgatcacctt caccaccggt ttcgagcgcg agggcttctc cgagatcgac gtcgcggtgg cttcggcaga ggccatcggt gcccgtcaca tcgccaaggt ggtcagcgcc gacgagttcg tcgccgccct gcccgagatc gtctggtacc tcgacgagcc ggtcgctgac ccagcgctgg taccgttgtt cttcgtcgcc cgcgaggccc gaaagcacgt caaagtggtg ttgtcgggcg aaggcgccga cgaactgttc ggcggctaca caatctatcg agaaccgctg tcgttgaggc cgtttgacta cctgcccaag ccactgcgcc ggtcgatggg aaaagtttcc aagccactgc cggagggcat gcgcggcaag agtctgctgc accgcggatc gctgacactc gaagagcgct actacggcaa tgcccgcagt ttctccggcg cgcagctgcg cgaagtactg cccgggttcc ggccggactg gacccacaca gatgtcacgg cgccggtcta cgccgaatcg gccggctggg atccggtggc gcgaatgcag cacatcgacc tgttcacctg gctgcgcggc gacattctgg tcaaggccga caagataacg atggccaact ccctggagct gcgggtgccg ttcctggacc cggaggtttt cgcggtggcc tcccggttgc cggcgggcgc caagatcacc cgtaccacca ccaagtacgc gctgcggcgc gcgctggagc ctattgtgcc cgcacacgtg ctgcaccggc ccaagctcgg gttcccggtc ccgatccggc attggctgcg tgccggcgag ctgctggagt gggcgtatgc gacggtgggc tcgtcgcagg ccggtcactt ggttgacatc gccgccgtgt atcgcatgct cgacgagcac cggtgcggca gcagcgacca cagccgccgg ctgtggacca tgctgatctt tatgctgtgg cacgcgatct tcgtcgagca cagcgtggtg ccccagatca gcgagccgca gtaccccgtc cagttgtaa

Details for MytuD.18530.a.B1.GE36552

HARVESTED ON:	1/9/2013
SEQUENCED ON:	1/7/2013
EXPECTED MW:	73kDa
OBSERVED MW:	74kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	86
PERCENT COVERAGE:	57

Validated AA Sequence

Validated NT Sequence

attttgntta nctttaagaa ggagatatac catggctcac caccaccacc accatatggt gtgtggactg ctggccttcg tcgcggcccc ggccggtgct gcggggcccg aaggtgccga cgctgccagc gccatcgccc gcgcatcgca tttgatgcgc caccgcgggc ccgatgaatc gggcacctgg cacgccgtcg atggcgcctc cggaggcgtc gtgttcgggt tcaaccgact gtccatcatc gacatcgcgc actcgcatca gccgctgcgg tgggggccgc cggaggctcc ggaccgctac gtgctggtgt tcaacggcga gatctacaac tacttggagc tgcgtgacga gctgcgcacc cagcacggcg ctgtgttcgc caccgacggc gacggtgagg cgatcctcgc cggctatcac cactggggca ccgaggtgct gcagcggttg cgcggcatgt tcgcattcgc gctgtgggac accgtcaccc gcgaattgtt ctgcgcgcga gatccgttcg gcatcaagcc gttgtttatc gccaccggag ccggcggcac ggcggtggcc agtgagaaga aatgcctgct ggacctcgtc gagttggtgg ggttcgacac cgagatcgac catcgggcgt tgcagcacta caccgtcctg cagtacgtgc cggaacccga gacactgcac cgtggggtac gtcggctgga atcaggctgc ttcgcccgga tccgtgccga ccagctcgcg ccggtgatca cccgttattt cgtgccgcga tttgcggccn gtccgatcac caacgacaac gaccaggccc gctatgacga gatcacggca gtgcttgagg actcngtggc caagcatatg cgcgccgatg tcaccgtcgg cgcgtttctg tccnggggta tcgactccac ggcnatcgcg gcgctggcca tccggcacaa tccgcggntg atcnccttca ccaccggttt cgagcgcgag ggcttctccn anatcgacgt cncgnnngnt tcgnnngagn natcggtgcn nntcncatcn cnngtngnca gcnncnacna ntnntnnncn cctgncnnan ntcnnnnggn ncctcnnnnn nnnncnntga nccnncnntn gnncnnnnnn nnnnnnnnnc nnnnngnnnn aannncntaa nnnnnnntnn ngnnnnnnnn nnnnnnnnnn tngnnnnnnn nnnnnntnnn nnnnnntnnn nnnnnnnc

Expected Protein Sequence

MAHHHHHHMV CGLLAFVAAP AGAAGPEGAD AASAIARASH LMRHRGPDES GTWHAVDGAS GGVVFGFNRL SIIDIAHSHQ PLRWGPPEAP DRYVLVFNGE IYNYLELRDE LRTQHGAVFA TDGDGEAILA GYHHWGTEVL QRLRGMFAFA LWDTVTRELF CARDPFGIKP LFIATGAGGT AVASEKKCLL DLVELVGFDT EIDHRALQHY TVLQYVPEPE TLHRGVRRLE SGCFARIRAD QLAPVITRYF VPRFAASPIT NDNDQARYDE ITAVLEDSVA KHMRADVTVG AFLSGGIDST AIAALAIRHN PRLITFTTGF EREGFSEIDV AVASAEAIGA RHIAKVVSAD EFVAALPEIV WYLDEPVADP ALVPLFFVAR EARKHVKVVL SGEGADELFG GYTIYREPLS LRPFDYLPKP LRRSMGKVSK PLPEGMRGKS LLHRGSLTLE ERYYGNARSF SGAQLREVLP GFRPDWTHTD VTAPVYAESA GWDPVARMQH IDLFTWLRGD ILVKADKITM ANSLELRVPF LDPEVFAVAS RLPAGAKITR TTTKYALRRA LEPIVPAHVL HRPKLGFPVP IRHWLRAGEL LEWAYATVGS SQAGHLVDIA AVYRMLDEHR CGSSDHSRRL WTMLIFMLWH AIFVEHSVVP QISEPQYPVQ L

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtgt gtggactgct ggccttcgtc gcggccccgg ccggtgctgc ggggcccgaa ggtgccgacg ctgccagcgc catcgcccgc gcatcgcatt tgatgcgcca ccgcgggccc gatgaatcgg gcacctggca cgccgtcgat ggcgcctccg gaggcgtcgt gttcgggttc aaccgactgt ccatcatcga catcgcgcac tcgcatcagc cgctgcggtg ggggccgccg gaggctccgg accgctacgt gctggtgttc aacggcgaga tctacaacta cttggagctg cgtgacgagc tgcgcaccca gcacggcgct gtgttcgcca ccgacggcga cggtgaggcg atcctcgccg gctatcacca ctggggcacc gaggtgctgc agcggttgcg cggcatgttc gcattcgcgc tgtgggacac cgtcacccgc gaattgttct gcgcgcgaga tccgttcggc atcaagccgt tgtttatcgc caccggagcc ggcggcacgg cggtggccag tgagaagaaa tgcctgctgg acctcgtcga gttggtgggg ttcgacaccg agatcgacca tcgggcgttg cagcactaca ccgtcctgca gtacgtgccg gaacccgaga cactgcaccg tggggtacgt cggctggaat caggctgctt cgcccggatc cgtgccgacc agctcgcgcc ggtgatcacc cgttatttcg tgccgcgatt tgcggccagt ccgatcacca acgacaacga ccaggcccgc tatgacgaga tcacggcagt gcttgaggac tcggtggcca agcatatgcg cgccgatgtc accgtcggcg cgtttctgtc cgggggtatc gactccacgg ccatcgcggc gctggccatc cggcacaatc cgcggctgat caccttcacc accggtttcg agcgcgaggg cttctccgag atcgacgtcg cggtggcttc ggcagaggcc atcggtgccc gtcacatcgc caaggtggtc agcgccgacg agttcgtcgc cgccctgccc gagatcgtct ggtacctcga cgagccggtc gctgacccag cgctggtacc gttgttcttc gtcgcccgcg aggcccgaaa gcacgtcaaa gtggtgttgt cgggcgaagg cgccgacgaa ctgttcggcg gctacacaat ctatcgagaa ccgctgtcgt tgaggccgtt tgactacctg cccaagccac tgcgccggtc gatgggaaaa gtttccaagc cactgccgga gggcatgcgc ggcaagagtc tgctgcaccg cggatcgctg acactcgaag agcgctacta cggcaatgcc cgcagtttct ccggcgcgca gctgcgcgaa gtactgcccg ggttccggcc ggactggacc cacacagatg tcacggcgcc ggtctacgcc gaatcggccg gctgggatcc ggtggcgcga atgcagcaca tcgacctgtt cacctggctg cgcggcgaca ttctggtcaa ggccgacaag ataacgatgg ccaactccct ggagctgcgg gtgccgttcc tggacccgga ggttttcgcg gtggcctccc ggttgccggc gggcgccaag atcacccgta ccaccaccaa gtacgcgctg cggcgcgcgc tggagcctat tgtgcccgca cacgtgctgc accggcccaa gctcgggttc ccggtcccga tccggcattg gctgcgtgcc ggcgagctgc tggagtgggc gtatgcgacg gtgggctcgt cgcaggccgg tcacttggtt gacatcgccg ccgtgtatcg catgctcgac gagcaccggt gcggcagcag cgaccacagc cgccggctgt ggaccatgct gatctttatg ctgtggcacg cgatcttcgt cgagcacagc gtggtgcccc agatcagcga gccgcagtac cccgtccagt tgtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat