SSGCID: Target Page

MytuD.18401.a

Divalent cation transporter, MgtE family (POSSIBLE Mg2+ TRANSPORT TRANSMEMBRANE PROTEIN MGTE)

CENTER ID:	MytuD.18401.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.18401.a.B2.GE36548	soluble domain		1	291

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|83332.12.peg.399
RefSeq:	NP_214876.1
TubercuList:	Rv0362
MTB Network Portal:	Rv0362
UniProt:	O06312

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSIRPAENST LDIRHVIGIG TPKAVDLWLD VVTELPDRAR ELGSLSKAEL GKLGPLLDGT NAVELFESID DKLAAEALHA MDPSLAATFL EALDSDHAAN ILREFKEPKR EALLTLLPLE RAMVLRGLLS WPEDCAAAHM VPETLTVRPN MTVSQAVASV RERASGLRSD ARTTAYVYVT DADSHLLGVI AFRALVLANP EQRVRELMGD DLIVVSPLTD KELAAQTIMG HNLMAVPVVD ADNRLLGIIA EDEAIDIAEE EATEDAERQG GSAPLEVPYL RASPWLLWRK RVVWLLVLFA AEAYTGSVLR AFSDEMEAVI ALAFFIPLLI GTGGNTGTQI ATTLVRAMAT GQVRFRDVPA VLAKELSTGV LVGLTMAAAA VVRAWTLGVG PQVTLTVALT VAAIVVWSSL VAAVLPPLLK KLRIDPAIVS GPMIATIVDG TGLLIYFLVA HLTLTELHGL

NT Sequence

gtgagcatcc gccccgccga gaactcaaca ctcgacatcc gccacgtcat cggtatcggc accccgaaag ccgtcgattt gtggctcgac gtcgtcaccg agctgccgga tcgcgcccgc gaactcgggt cgttatccaa agccgaactc ggaaagcttg gcccactgct cgacggcacc aacgccgtcg agctattcga gtcgatcgac gacaagctgg ccgcagaggc actgcacgcg atggatccgt cgctggccgc caccttcctc gaggccctcg actccgacca cgccgccaac atcctgcgcg aattcaagga gcccaagcgg gaggcgctgc tgacgttgct accgctggag cgggcgatgg tgctgcgtgg cttgttgagc tggccggagg actgcgccgc ggcccacatg gtgcccgaaa cgctgaccgt acgcccgaac atgacggtgt cgcaggccgt cgccagcgtg cgggaacgcg cctcgggcct gcgcagcgat gcacgaacca ccgcctacgt ctatgtgaca gacgccgact cccacctgct gggtgtgatc gcctttcgcg ccctggtgct ggccaatccc gaacagcgag tccgtgagct gatgggtgac gacctcatcg tcgtgtcgcc gttgactgac aaggagctcg cggcgcagac aatcatgggc cacaacctga tggcggttcc cgtcgtcgat gccgacaacc ggctactggg catcatcgcc gaagacgaag ccatcgacat tgccgaggag gaagcaaccg aagacgccga gcgccagggt gggtcggccc cgctcgaggt gccctacctg cgggcgtcgc cgtggctgct atggcgcaag cgggtcgtct ggctcctggt actttttgct gccgaggcct acaccggcag cgtcctgcgg gcgttctccg acgaaatgga ggcggtgata gcgctcgcgt tcttcatccc actgctgatc ggcaccggcg gcaacaccgg cacccagatc gccaccactc tggtccgcgc gatggccacc ggtcaggtcc ggtttcgcga tgtgcctgcg gtgttagcca aggagctgtc aaccggtgtg ctggtcggcc tcactatggc cgccgccgcg gtggtgcgcg cctggacatt gggcgtgggc ccgcaggtga ccctgacggt cgcgctgacg gtggccgcca tcgtggtgtg gtcgtcgctg gtggctgccg tccttccgcc gctgctgaag aagttgcgca tcgacccggc catcgtttcg gggccgatga tcgccaccat cgttgacggc acgggtctgc tcatctactt cctggtcgcg cacctgacgc tgaccgagct gcacggcttg tga

Details for MytuD.18401.a.B2.GE36548

HARVESTED ON:	1/9/2013
SEQUENCED ON:	1/7/2013
EXPECTED MW:	33kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMV SIRPAENSTL DIRHVIGIGT PKAVDLWLDV VTELPDRARE LGSLSKAELG KLGPLLDGTN AVELFESIDD KLAAEALHAM DPSLAATFLE ALDSDHAANI LREFKEPKRE ALLTLLPLER AMVLRGLLSW PEDCAAAHMV PETLTVRPNM TVSQAVASVR ERASGLRSDA RTTAYVYVTD ADSHLLGVIA FRALVLANPE QRVRELMGDD LIVVSPLTDK ELAAQTIMGH NLMAVPVVDA DNRLLGIIAE DEAIDIAEEE ATEDAERQGG SAPLEVPYLR ASPWLLWRKR

Validated NT Sequence

tactcactta ccgcttgcgc catagcagcc acggcgacgc ccgcaggtag ggcacctcga gcggggccga cccaccctgg cgctcggcgt cttcggttgc ttcctcctcg gcaatgtcga tggcttcgtc ttcggcgatg atgcccagta gccggttgtc ggcatcgacg acgggaaccg ccatcaggtt gtggcccatg attgtctgcg ccgcgagctc cttgtcagtc aacggcgaca cgacgatgag gtcgtcaccc atcagctcac ggactcgctg ttcgggattg gccagcacca gggcgcgaaa ggcgatcaca cccagcaggt gggagtcggc gtctgtcaca tagacgtagg cggtggttcg tgcatcgctg cgcaggcccg aggcgcgttc ccgcacgctg gcgacggcct gcgacaccgt catgttcggg cgtacggtca gcgtttcggg caccatgtgg gccgcggcgc agtcctccgg ccagctcaac aagccacgca gcaccatcgc ccgctccagc ggtagcaacg tcagcagcgc ctcccgcttg ggctccttga attcgcgcag gatgttggcg gcgtggtcgg agtcgagggc ctcgaggaag gtggcggcca gcgacggatc catcgcgtgc agtgcctctg cggccagctt gtcgtcgatc gactcgaata gctcgacggc gttggtgccg tcgagcagtg ggccaagctt tccgagttcg gctttggata acgacccgag ttcgcgggcg cgatccggca gctcggtgac gacgtcgagc cacaaatcga cggctttcgg ggtgccgata ccgatgacgt ggcggatgtc gagtgttgag ttctcggcgg ggcggatgct caccatatgg tggtggtggt ggtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtga gcatccgccc cgccgagaac tcaacactcg acatccgcca cgtcatcggt atcggcaccc cgaaagccgt cgatttgtgg ctcgacgtcg tcaccgagct gccggatcgc gcccgcgaac tcgggtcgtt atccaaagcc gaactcggaa agcttggccc actgctcgac ggcaccaacg ccgtcgagct attcgagtcg atcgacgaca agctggccgc agaggcactg cacgcgatgg atccgtcgct ggccgccacc ttcctcgagg ccctcgactc cgaccacgcc gccaacatcc tgcgcgaatt caaggagccc aagcgggagg cgctgctgac gttgctaccg ctggagcggg cgatggtgct gcgtggcttg ttgagctggc cggaggactg cgccgcggcc cacatggtgc ccgaaacgct gaccgtacgc ccgaacatga cggtgtcgca ggccgtcgcc agcgtgcggg aacgcgcctc gggcctgcgc agcgatgcac gaaccaccgc ctacgtctat gtgacagacg ccgactccca cctgctgggt gtgatcgcct ttcgcgccct ggtgctggcc aatcccgaac agcgagtccg tgagctgatg ggtgacgacc tcatcgtcgt gtcgccgttg actgacaagg agctcgcggc gcagacaatc atgggccaca acctgatggc ggttcccgtc gtcgatgccg acaaccggct actgggcatc atcgccgaag acgaagccat cgacattgcc gaggaggaag caaccgaaga cgccgagcgc cagggtgggt cggccccgct cgaggtgccc tacctgcggg cgtcgccgtg gctgctatgg cgcaagcggt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat