SSGCID: Target Page

MytuD.18397.a

Putative uncharacterized protein

CENTER ID:	MytuD.18397.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.18397.a.A1.GE34400	full length		1	421

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_237654
PATRIC ID:	fig\|83332.12.peg.1340
RefSeq:	NP_215710.1
TubercuList:	Rv1194c
MTB Network Portal:	Rv1194c
UniProt:	O05296

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAWQQPSPRI RELIREGARI ALNPSPEWIE ELDRATIAAN PAIANDPVLA KVVQTANRAN LVYWAAANLR DPGARVPANL GTEPLRMARD LVRRGLDTVA FNIYRTGEHI GWRFWMGIAF ELTSDPQELR ELLDVSARSV NDFIEATLTG IAAQVQSEHD ELTRSTHAER LEVVGLILDG APISPERAEA KLGYPLSRAH TAAIIWSDEL DGDHSYLDRA ADLFCHAVGS TRPLTVVAGA ASRWAWVTDA DGLDIDTVQA AVDNAPGARI AIGTTANGVE GFRRSHLEAL ITQRTLSRLR STQRVAFFAD VKMVALISQN PDAASEFITS TLGDLESASP DLQTALLTFI NEQCNASRAA KRLHTHRNTF LRRLESAQRL LPRPLDHTSV HVAVALEALQ WRGNKAHALS SPGRRSNSVP A

NT Sequence

gtggcatggc aacaaccttc gccgcgaatt cgtgagctga tccgcgaggg agcacgcatc gccctcaatc ccagcccgga gtggatcgag gaactggacc gtgccacgat tgcggccaat ccggcgatcg ccaacgatcc ggtgcttgcg aaggttgtcc agacggccaa tcgcgccaac ctggtgtact gggctgcggc caacctgcgt gaccctggtg ctcgggtgcc cgccaatctc ggcaccgagc cactgcggat ggcccgagac ctggtgcgcc gggggctgga cacagtagcg ttcaacatct atcgaaccgg tgagcacatc gggtggcggt tctggatggg gattgcgttc gaactcactt ccgaccccca agagctgcgc gagctgctag acgtgtcggc ccgctccgtc aacgacttca tcgaggccac gctgaccggt atcgccgcgc aagtgcagtc ggaacacgac gaactgaccc gcagcaccca tgccgaacgc ctcgaggtcg tcgggctgat tctcgatggc gcccctatca gccccgaacg ggccgaagcg aaattgggct acccgctgag tcgtgcccat accgctgcca tcatctggag cgacgagctc gacggtgacc acagctacct cgaccgggcg gctgatctgt tctgccacgc cgtgggatcg acacgaccgc tgacggtggt cgccggggcg gcgagccgct gggcgtgggt gaccgacgcc gacggactcg acatcgacac agtgcaggcg gcggtggaca acgcacccgg ggcgcgcatc gccatcggga ccaccgccaa cggggtggag gggttccggc gcagccacct cgaagcgctc atcacccagc gcaccttgtc acggctacgg tcgacccagc gggtcgcctt ctttgccgac gttaagatgg tcgcattgat cagccaaaat ccagatgctg caagcgaatt catcacgagt acgcttggag acctcgagtc tgccagccca gatctacaaa cggccctgct gaccttcatc aacgagcagt gcaacgcctc ccgggccgca aaacgtctcc acactcaccg caatacattt ctgcgcagac tcgagtccgc tcagcggctg ctaccgcgac cgctcgacca cacctccgtc catgtggccg tcgccctgga agccctacag tggcgcggca acaaggccca tgcgctaagc tcaccgggcc gtcggagtaa cagtgtgcca gcgtaa

Details for MytuD.18397.a.A1.GE34400

HARVESTED ON:	1/10/2012
SEQUENCED ON:	1/25/2012
EXPECTED MW:	48kDa
OBSERVED MW:	48kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	97
PERCENT COVERAGE:	57

Validated AA Sequence

Validated NT Sequence

ttnnttnnct ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggtggca tggcaacaac cttcgccgcg aattcgtgag ctgatccgcg agggagcacg catcgccctc aatcccagcc cggagtggat cgaggaactg gaccgtgcca cgattgcggc caatccggcg atcgccaacg atccggtgct tgcgaaggtt gtccagacgg ccaatcgcgc caacctggtg tactgggctg cggccaacct gcgtgaccct ggtgctcggg tgcccgccaa tctcggcacc gagccactgc ggatggcccg agacctggtg cgccgggggc tggacacagt agcgttcaac atctatcgaa ccggtgagca catcgggtgg cggttctgga tggggattgc gttcgaactc acttccgacc cccaagagct gcgcgagctg ctagacgtgt cggcccgctc cgtcaacgac ttcatcgagg ccacgctgac cggtatcgcc gcgcaagtgc agtcggaaca cgacgaactg acccgcagca cccatgccga acgcctcgag gtcgtcgggc tgattctcga tggcncnnct atcagccccg aacgggccga agcgaaattg ggctacccgc tgagtcgtgc ccataccgct gccatcatct ggagcgacga gctcgacggt gaccacagct acctcgaccg ggcggctgat ctgttctgcc acgccgtgga ncgacacgac cgctgacggt ggtcgccngg gcggcgagcc gctnggcgtg ggtgaccgac gccnannnct cgacatcgac ncagtgcnnn nggcggtgga caacncaccn nggcgcgcat cgccntcggg accnccgccn nggggtggan gggttncgnn ncagccnnct cnaagcnntn atnanccann nnnncttgnn acggctnnng ncnannnnnn nggnngncnn nnntt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVAWQQPSP RIRELIREGA RIALNPSPEW IEELDRATIA ANPAIANDPV LAKVVQTANR ANLVYWAAAN LRDPGARVPA NLGTEPLRMA RDLVRRGLDT VAFNIYRTGE HIGWRFWMGI AFELTSDPQE LRELLDVSAR SVNDFIEATL TGIAAQVQSE HDELTRSTHA ERLEVVGLIL DGAPISPERA EAKLGYPLSR AHTAAIIWSD ELDGDHSYLD RAADLFCHAV GSTRPLTVVA GAASRWAWVT DADGLDIDTV QAAVDNAPGA RIAIGTTANG VEGFRRSHLE ALITQRTLSR LRSTQRVAFF ADVKMVALIS QNPDAASEFI TSTLGDLESA SPDLQTALLT FINEQCNASR AAKRLHTHRN TFLRRLESAQ RLLPRPLDHT SVHVAVALEA LQWRGNKAHA LSSPGRRSNS VPA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggcatgg caacaacctt cgccgcgaat tcgtgagctg atccgcgagg gagcacgcat cgccctcaat cccagcccgg agtggatcga ggaactggac cgtgccacga ttgcggccaa tccggcgatc gccaacgatc cggtgcttgc gaaggttgtc cagacggcca atcgcgccaa cctggtgtac tgggctgcgg ccaacctgcg tgaccctggt gctcgggtgc ccgccaatct cggcaccgag ccactgcgga tggcccgaga cctggtgcgc cgggggctgg acacagtagc gttcaacatc tatcgaaccg gtgagcacat cgggtggcgg ttctggatgg ggattgcgtt cgaactcact tccgaccccc aagagctgcg cgagctgcta gacgtgtcgg cccgctccgt caacgacttc atcgaggcca cgctgaccgg tatcgccgcg caagtgcagt cggaacacga cgaactgacc cgcagcaccc atgccgaacg cctcgaggtc gtcgggctga ttctcgatgg cgcccctatc agccccgaac gggccgaagc gaaattgggc tacccgctga gtcgtgccca taccgctgcc atcatctgga gcgacgagct cgacggtgac cacagctacc tcgaccgggc ggctgatctg ttctgccacg ccgtgggatc gacacgaccg ctgacggtgg tcgccggggc ggcgagccgc tgggcgtggg tgaccgacgc cgacggactc gacatcgaca cagtgcaggc ggcggtggac aacgcacccg gggcgcgcat cgccatcggg accaccgcca acggggtgga ggggttccgg cgcagccacc tcgaagcgct catcacccag cgcaccttgt cacggctacg gtcgacccag cgggtcgcct tctttgccga cgttaagatg gtcgcattga tcagccaaaa tccagatgct gcaagcgaat tcatcacgag tacgcttgga gacctcgagt ctgccagccc agatctacaa acggccctgc tgaccttcat caacgagcag tgcaacgcct cccgggccgc aaaacgtctc cacactcacc gcaatacatt tctgcgcaga ctcgagtccg ctcagcggct gctaccgcga ccgctcgacc acacctccgt ccatgtggcc gtcgccctgg aagccctaca gtggcgcggc aacaaggccc atgcgctaag ctcaccgggc cgtcggagta acagtgtgcc agcgtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa