MytuD.18395.a

Uncharacterized protein Rv2004c/MT2060

CENTER ID: MytuD.18395.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18395.a.B1.GE36554 full length 1 498
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18395.a.B1.PW37323 full length 1 498
MytuD.18395.a.B1.PW37324 full length 1 498

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.2239
RefSeq: NP_216520.1
TubercuList: Rv2004c
MTB Network Portal: Rv2004c
UniProt: P9WLN3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDSPTNDGTC DAHPVTDEPF IDVRETHTAV VVLAGDRAFK AKKPVVTDFC DFRTAEQRER ACIREFELNS RLAAQSYLGI AHLSDPSGGH AEPVVVMRRY RDKQRLASMV TAGLPVEGAL DAIAEVLARF HQRAQRNRCI DTQGEVGAVA RRWHENLAEL RHHADKVVSG DVIRRIEHMV DEFVSGREVL FAGRIKEGCI VDGHADLLAD DIFLVDGEPA LLDCLEFEDE LRYLDRIDDA AFLAMDLEFL GRKDLGDYFL AGYAVRSGDT APASLRDFYI AYRAVVRAKV ECVRFSQGKP EAAADAVRHL IIATQHLQHA TVRLALVGGN PGTGKSTLAR GVAELVGAQV ISTDDVRRRL RDCGVITGEP GVLDSGLYSR ANVVAVYQEA LRKARLLLGS GHSVILDGTW GDPQMRACAR RLAADTHSAI VEFRCSATVD VMADRIVARA GGNSDATAEI AAALAARQAD WDTGHRIDTA GPRERSVGQA YHIWRSAI
NT Sequence
atggactcac cgaccaacga cggcacgtgt gacgcccatc ctgtgacaga tgagcctttc atcgatgtgc gcgaaaccca caccgcagtg gttgtgttgg ccggcgaccg ggcgttcaag gcgaaaaagc ctgtggtgac ggatttctgc gacttccgca cggcggaaca gcgtgagcgc gcctgtatcc gggaattcga gttgaatagt cggctggccg cgcagagcta cttgggcatt gcccatctca gcgatccaag cggcgggcac gccgaaccgg tcgtcgtcat gcggcgttac cgcgacaagc agcggctggc gtcgatggtg accgccggcc tgccggtcga gggtgccctg gacgcgatcg ctgaggtgtt ggcccggttt caccagcgcg cacagcgtaa ccggtgcatc gatactcaag gcgaggtcgg tgcggtggcg cggcgttggc acgaaaacct ggccgaactt cgccaccacg cggacaaagt ggtctccggg gatgtgattc ggcggattga acacatggtc gacgaattcg tttccgggcg cgaggttttg ttcgccggcc ggatcaaaga aggttgtatt gtagacggcc acgcggatct actggccgat gacatcttct tggtggatgg cgaaccggcg ctgctggatt gcttggagtt cgaagacgag cttcgctacc tcgaccgcat cgacgacgcc gcctttttgg ccatggatct ggagttcttg ggccgcaaag atcttgggga ctacttcctg gccggctatg cggtgcgttc gggtgatacc gccccggcgt cgctgcgcga cttctacatt gcttatcgcg cggtggtgcg cgcgaaagtc gagtgcgtgc gattctccca gggcaaaccg gaggccgctg cggacgccgt gcgccacctg atcatcgcca cccaacacct acagcacgct acggtccggt tggcgctggt cggtggcaat ccgggcaccg gcaagtcgac cctggcccgc ggggtcgccg aactggtggg ggcgcaggtg atctcaaccg acgatgtgcg ccggcggcta cgcgattgcg gagtaatcac cggggagccc ggcgttttgg actccgggct ctacagtcgt gccaacgttg tggcggtcta ccaggaggcg ctgcgcaaag cccgcctgct cttgggtagt gggcactcag tgatcctcga cggtacctgg ggtgatccgc agatgcgcgc ctgcgcccgg cgtctcgcgg cggacacgca ctcggcgatt gtcgaattca ggtgttcggc gacggttgac gtgatggccg acagaatcgt tgctagagcc gggggcaact ccgacgccac cgccgagatc gcggcggcct tggccgcccg gcaagcggac tgggacaccg ggcaccggat cgacacggcg gggccgcgtg agcgctccgt gggacaggcc taccacatct ggcgcagcgc gatctga
Details for MytuD.18395.a.B1.GE36554
HARVESTED ON: 1/9/2013
SEQUENCED ON: 1/7/2013
EXPECTED MW: 55kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 79
Validated AA Sequence
MRRYRDKQRL ASMVTAGLPV EGALDAIAEV LARFHQRAQR NRCIDTQGEV GAVARRWHEN LAELRHHADK VVSGDVIRRI EHMVDEFVSG REVLFAGRIK EGCIVDGHAD LLADDIFLVD GEPALLDCLE FEDELRYLDR IDDAAFLAMD LEFLGRKDLG DYFLAGYAVR SGDTAPASLR DFYIAYRAVV RAKVECVRFS QGKPEAAADA VRHLIIATQH LQHATVRLAL VGGNPGTGKS TLARGVAELV GAQVISTDDV RRRLRDCGVI TGEPGVLDSG LYSRANVVAV YQEALRKARL LLGSGHSVIL DGTWGDPQMR ACARRLAADT HSAIVEFRCS ATVDVMADRI VARAGGNSDA TAEIAAALAA RQADWDTGHR IDTAGPRERS VGQAYHIWRS AI
Validated NT Sequence
atcttactca cttagatcgc gctgcgccag atgtggtagg cctgtcccac ggagcgctca cgcggccccg ccgtgtcgat ccggtgcccg gtgtcccagt ccgcttgccg ggcggccaag gccgccgcga tctcggcggt ggcgtcggag ttgcccccgg ctctagcaac gattctgtcg gccatcacgt caaccgtcgc cgaacacctg aattcgacaa tcgccgagtg cgtgtccgcc gcgagacgcc gggcgcaggc gcgcatctgc ggatcacccc aggtaccgtc gaggatcact gagtgcccac tacccaagag caggcgggct ttgcgcagcg cctcctggta gaccgccaca acgttggcac gactgtagag cccggagtcc aaaacgccgg gctccccggt gattactccg caatcgcgta gccgccggcg cacatcgtcg gttgagatca cctgcgcccc caccagttcg gcgaccccgc gggccagggt cgacttgccg gtgcccggat tgccaccgac cagcgccaac cggaccgtag cgtgctgtag gtgttgggtg gcgatgatca ggtggcgcac ggcgtccgca gcggcctccg gtttgccctg ggagaatcgc acgcactcga ctttcgcgcg caccaccgcg cgataagcaa tgtagaagtc gcgcagcgac gccggggcgg tatcacccga acgcaccgca tagccggcca ggaagtagtc cccaagatct ttgcggccca agaactccag atccatggcc aaaaaggcgg cgtcgtcgat gcggtcgagg tagcgaagct cgtcttcgaa ctccaagcaa tccagcagcg ccggttcgcc atccaccaag aagatgtcat cggccagtag atccgcgtgg ccgtctacaa tacaaccttc tttgatccgg ccggcgaaca aaacctcgcg cccggaaacg aattcgtcga ccatgtgttc aatccgccga atcacatccc cggagaccac tttgtccgcg tggtggcgaa gttcggccag gttttcgtgc caacgccgcg ccaccgcacc gacctcgcct tgagtatcga tgcaccggtt acgctgtgcg cgctggtgaa accgggccaa cacctcagcg atcgcgtcca gggcaccctc gaccggcagg ccggcggtca ccatcgacgc cagccgctgc ttgtcgcggt aacgccgcat gacgacgacc ggttcggcgt gcccgccgct tggatcgctg agatgggcaa tgcccaagta gctctgcgcg gccagccgac tattcaactc gaattcccgg atacaggcgc gctcacgctg ttccgccgtg cggaagtcgc agaaatccgt caccacaggc tttttcgcct tgaacgcccg gtcgccggcc aacacaacca ctgcggtgtg ggtttcgcgc acatcgatga aaggctcatc tgtcacagga tgggcgtcac acgtgccgtc gttggtcggt gagtc
Expected Protein Sequence
MAHHHHHHMD SPTNDGTCDA HPVTDEPFID VRETHTAVVV LAGDRAFKAK KPVVTDFCDF RTAEQRERAC IREFELNSRL AAQSYLGIAH LSDPSGGHAE PVVVMRRYRD KQRLASMVTA GLPVEGALDA IAEVLARFHQ RAQRNRCIDT QGEVGAVARR WHENLAELRH HADKVVSGDV IRRIEHMVDE FVSGREVLFA GRIKEGCIVD GHADLLADDI FLVDGEPALL DCLEFEDELR YLDRIDDAAF LAMDLEFLGR KDLGDYFLAG YAVRSGDTAP ASLRDFYIAY RAVVRAKVEC VRFSQGKPEA AADAVRHLII ATQHLQHATV RLALVGGNPG TGKSTLARGV AELVGAQVIS TDDVRRRLRD CGVITGEPGV LDSGLYSRAN VVAVYQEALR KARLLLGSGH SVILDGTWGD PQMRACARRL AADTHSAIVE FRCSATVDVM ADRIVARAGG NSDATAEIAA ALAARQADWD TGHRIDTAGP RERSVGQAYH IWRSAI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggact caccgaccaa cgacggcacg tgtgacgccc atcctgtgac agatgagcct ttcatcgatg tgcgcgaaac ccacaccgca gtggttgtgt tggccggcga ccgggcgttc aaggcgaaaa agcctgtggt gacggatttc tgcgacttcc gcacggcgga acagcgtgag cgcgcctgta tccgggaatt cgagttgaat agtcggctgg ccgcgcagag ctacttgggc attgcccatc tcagcgatcc aagcggcggg cacgccgaac cggtcgtcgt catgcggcgt taccgcgaca agcagcggct ggcgtcgatg gtgaccgccg gcctgccggt cgagggtgcc ctggacgcga tcgctgaggt gttggcccgg tttcaccagc gcgcacagcg taaccggtgc atcgatactc aaggcgaggt cggtgcggtg gcgcggcgtt ggcacgaaaa cctggccgaa cttcgccacc acgcggacaa agtggtctcc ggggatgtga ttcggcggat tgaacacatg gtcgacgaat tcgtttccgg gcgcgaggtt ttgttcgccg gccggatcaa agaaggttgt attgtagacg gccacgcgga tctactggcc gatgacatct tcttggtgga tggcgaaccg gcgctgctgg attgcttgga gttcgaagac gagcttcgct acctcgaccg catcgacgac gccgcctttt tggccatgga tctggagttc ttgggccgca aagatcttgg ggactacttc ctggccggct atgcggtgcg ttcgggtgat accgccccgg cgtcgctgcg cgacttctac attgcttatc gcgcggtggt gcgcgcgaaa gtcgagtgcg tgcgattctc ccagggcaaa ccggaggccg ctgcggacgc cgtgcgccac ctgatcatcg ccacccaaca cctacagcac gctacggtcc ggttggcgct ggtcggtggc aatccgggca ccggcaagtc gaccctggcc cgcggggtcg ccgaactggt gggggcgcag gtgatctcaa ccgacgatgt gcgccggcgg ctacgcgatt gcggagtaat caccggggag cccggcgttt tggactccgg gctctacagt cgtgccaacg ttgtggcggt ctaccaggag gcgctgcgca aagcccgcct gctcttgggt agtgggcact cagtgatcct cgacggtacc tggggtgatc cgcagatgcg cgcctgcgcc cggcgtctcg cggcggacac gcactcggcg attgtcgaat tcaggtgttc ggcgacggtt gacgtgatgg ccgacagaat cgttgctaga gccgggggca actccgacgc caccgccgag atcgcggcgg ccttggccgc ccggcaagcg gactgggaca ccgggcaccg gatcgacacg gcggggccgc gtgagcgctc cgtgggacag gcctaccaca tctggcgcag cgcgatctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for MytuD.18395.a.B1.PW37323
PURIFICATION DATe: 7/1/2013
CONCENTRATION: 5.2mg/ml
OBSERVED MW: 55kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 79
Protocol Notes
notes unavailable
Validated AA Sequence
MRRYRDKQRL ASMVTAGLPV EGALDAIAEV LARFHQRAQR NRCIDTQGEV GAVARRWHEN LAELRHHADK VVSGDVIRRI EHMVDEFVSG REVLFAGRIK EGCIVDGHAD LLADDIFLVD GEPALLDCLE FEDELRYLDR IDDAAFLAMD LEFLGRKDLG DYFLAGYAVR SGDTAPASLR DFYIAYRAVV RAKVECVRFS QGKPEAAADA VRHLIIATQH LQHATVRLAL VGGNPGTGKS TLARGVAELV GAQVISTDDV RRRLRDCGVI TGEPGVLDSG LYSRANVVAV YQEALRKARL LLGSGHSVIL DGTWGDPQMR ACARRLAADT HSAIVEFRCS ATVDVMADRI VARAGGNSDA TAEIAAALAA RQADWDTGHR IDTAGPRERS VGQAYHIWRS AI
Validated NT Sequence
atcttactca cttagatcgc gctgcgccag atgtggtagg cctgtcccac ggagcgctca cgcggccccg ccgtgtcgat ccggtgcccg gtgtcccagt ccgcttgccg ggcggccaag gccgccgcga tctcggcggt ggcgtcggag ttgcccccgg ctctagcaac gattctgtcg gccatcacgt caaccgtcgc cgaacacctg aattcgacaa tcgccgagtg cgtgtccgcc gcgagacgcc gggcgcaggc gcgcatctgc ggatcacccc aggtaccgtc gaggatcact gagtgcccac tacccaagag caggcgggct ttgcgcagcg cctcctggta gaccgccaca acgttggcac gactgtagag cccggagtcc aaaacgccgg gctccccggt gattactccg caatcgcgta gccgccggcg cacatcgtcg gttgagatca cctgcgcccc caccagttcg gcgaccccgc gggccagggt cgacttgccg gtgcccggat tgccaccgac cagcgccaac cggaccgtag cgtgctgtag gtgttgggtg gcgatgatca ggtggcgcac ggcgtccgca gcggcctccg gtttgccctg ggagaatcgc acgcactcga ctttcgcgcg caccaccgcg cgataagcaa tgtagaagtc gcgcagcgac gccggggcgg tatcacccga acgcaccgca tagccggcca ggaagtagtc cccaagatct ttgcggccca agaactccag atccatggcc aaaaaggcgg cgtcgtcgat gcggtcgagg tagcgaagct cgtcttcgaa ctccaagcaa tccagcagcg ccggttcgcc atccaccaag aagatgtcat cggccagtag atccgcgtgg ccgtctacaa tacaaccttc tttgatccgg ccggcgaaca aaacctcgcg cccggaaacg aattcgtcga ccatgtgttc aatccgccga atcacatccc cggagaccac tttgtccgcg tggtggcgaa gttcggccag gttttcgtgc caacgccgcg ccaccgcacc gacctcgcct tgagtatcga tgcaccggtt acgctgtgcg cgctggtgaa accgggccaa cacctcagcg atcgcgtcca gggcaccctc gaccggcagg ccggcggtca ccatcgacgc cagccgctgc ttgtcgcggt aacgccgcat gacgacgacc ggttcggcgt gcccgccgct tggatcgctg agatgggcaa tgcccaagta gctctgcgcg gccagccgac tattcaactc gaattcccgg atacaggcgc gctcacgctg ttccgccgtg cggaagtcgc agaaatccgt caccacaggc tttttcgcct tgaacgcccg gtcgccggcc aacacaacca ctgcggtgtg ggtttcgcgc acatcgatga aaggctcatc tgtcacagga tgggcgtcac acgtgccgtc gttggtcggt gagtc
Expressed Protein Sequence
MAHHHHHHMD SPTNDGTCDA HPVTDEPFID VRETHTAVVV LAGDRAFKAK KPVVTDFCDF RTAEQRERAC IREFELNSRL AAQSYLGIAH LSDPSGGHAE PVVVMRRYRD KQRLASMVTA GLPVEGALDA IAEVLARFHQ RAQRNRCIDT QGEVGAVARR WHENLAELRH HADKVVSGDV IRRIEHMVDE FVSGREVLFA GRIKEGCIVD GHADLLADDI FLVDGEPALL DCLEFEDELR YLDRIDDAAF LAMDLEFLGR KDLGDYFLAG YAVRSGDTAP ASLRDFYIAY RAVVRAKVEC VRFSQGKPEA AADAVRHLII ATQHLQHATV RLALVGGNPG TGKSTLARGV AELVGAQVIS TDDVRRRLRD CGVITGEPGV LDSGLYSRAN VVAVYQEALR KARLLLGSGH SVILDGTWGD PQMRACARRL AADTHSAIVE FRCSATVDVM ADRIVARAGG NSDATAEIAA ALAARQADWD TGHRIDTAGP RERSVGQAYH IWRSAI
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGACT CACCGACCAA CGACGGCACG TGTGACGCCC ATCCTGTGAC AGATGAGCCT TTCATCGATG TGCGCGAAAC CCACACCGCA GTGGTTGTGT TGGCCGGCGA CCGGGCGTTC AAGGCGAAAA AGCCTGTGGT GACGGATTTC TGCGACTTCC GCACGGCGGA ACAGCGTGAG CGCGCCTGTA TCCGGGAATT CGAGTTGAAT AGTCGGCTGG CCGCGCAGAG CTACTTGGGC ATTGCCCATC TCAGCGATCC AAGCGGCGGG CACGCCGAAC CGGTCGTCGT CATGCGGCGT TACCGCGACA AGCAGCGGCT GGCGTCGATG GTGACCGCCG GCCTGCCGGT CGAGGGTGCC CTGGACGCGA TCGCTGAGGT GTTGGCCCGG TTTCACCAGC GCGCACAGCG TAACCGGTGC ATCGATACTC AAGGCGAGGT CGGTGCGGTG GCGCGGCGTT GGCACGAAAA CCTGGCCGAA CTTCGCCACC ACGCGGACAA AGTGGTCTCC GGGGATGTGA TTCGGCGGAT TGAACACATG GTCGACGAAT TCGTTTCCGG GCGCGAGGTT TTGTTCGCCG GCCGGATCAA AGAAGGTTGT ATTGTAGACG GCCACGCGGA TCTACTGGCC GATGACATCT TCTTGGTGGA TGGCGAACCG GCGCTGCTGG ATTGCTTGGA GTTCGAAGAC GAGCTTCGCT ACCTCGACCG CATCGACGAC GCCGCCTTTT TGGCCATGGA TCTGGAGTTC TTGGGCCGCA AAGATCTTGG GGACTACTTC CTGGCCGGCT ATGCGGTGCG TTCGGGTGAT ACCGCCCCGG CGTCGCTGCG CGACTTCTAC ATTGCTTATC GCGCGGTGGT GCGCGCGAAA GTCGAGTGCG TGCGATTCTC CCAGGGCAAA CCGGAGGCCG CTGCGGACGC CGTGCGCCAC CTGATCATCG CCACCCAACA CCTACAGCAC GCTACGGTCC GGTTGGCGCT GGTCGGTGGC AATCCGGGCA CCGGCAAGTC GACCCTGGCC CGCGGGGTCG CCGAACTGGT GGGGGCGCAG GTGATCTCAA CCGACGATGT GCGCCGGCGG CTACGCGATT GCGGAGTAAT CACCGGGGAG CCCGGCGTTT TGGACTCCGG GCTCTACAGT CGTGCCAACG TTGTGGCGGT CTACCAGGAG GCGCTGCGCA AAGCCCGCCT GCTCTTGGGT AGTGGGCACT CAGTGATCCT CGACGGTACC TGGGGTGATC CGCAGATGCG CGCCTGCGCC CGGCGTCTCG CGGCGGACAC GCACTCGGCG ATTGTCGAAT TCAGGTGTTC GGCGACGGTT GACGTGATGG CCGACAGAAT CGTTGCTAGA GCCGGGGGCA ACTCCGACGC CACCGCCGAG ATCGCGGCGG CCTTGGCCGC CCGGCAAGCG GACTGGGACA CCGGGCACCG GATCGACACG GCGGGGCCGC GTGAGCGCTC CGTGGGACAG GCCTACCACA TCTGGCGCAG CGCGATCTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT
Details for MytuD.18395.a.B1.PW37324
PURIFICATION DATe: 7/8/2013
CONCENTRATION: 5.2mg/ml
OBSERVED MW: 55kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 79
Protocol Notes
notes unavailable
Validated AA Sequence
MRRYRDKQRL ASMVTAGLPV EGALDAIAEV LARFHQRAQR NRCIDTQGEV GAVARRWHEN LAELRHHADK VVSGDVIRRI EHMVDEFVSG REVLFAGRIK EGCIVDGHAD LLADDIFLVD GEPALLDCLE FEDELRYLDR IDDAAFLAMD LEFLGRKDLG DYFLAGYAVR SGDTAPASLR DFYIAYRAVV RAKVECVRFS QGKPEAAADA VRHLIIATQH LQHATVRLAL VGGNPGTGKS TLARGVAELV GAQVISTDDV RRRLRDCGVI TGEPGVLDSG LYSRANVVAV YQEALRKARL LLGSGHSVIL DGTWGDPQMR ACARRLAADT HSAIVEFRCS ATVDVMADRI VARAGGNSDA TAEIAAALAA RQADWDTGHR IDTAGPRERS VGQAYHIWRS AI
Validated NT Sequence
atcttactca cttagatcgc gctgcgccag atgtggtagg cctgtcccac ggagcgctca cgcggccccg ccgtgtcgat ccggtgcccg gtgtcccagt ccgcttgccg ggcggccaag gccgccgcga tctcggcggt ggcgtcggag ttgcccccgg ctctagcaac gattctgtcg gccatcacgt caaccgtcgc cgaacacctg aattcgacaa tcgccgagtg cgtgtccgcc gcgagacgcc gggcgcaggc gcgcatctgc ggatcacccc aggtaccgtc gaggatcact gagtgcccac tacccaagag caggcgggct ttgcgcagcg cctcctggta gaccgccaca acgttggcac gactgtagag cccggagtcc aaaacgccgg gctccccggt gattactccg caatcgcgta gccgccggcg cacatcgtcg gttgagatca cctgcgcccc caccagttcg gcgaccccgc gggccagggt cgacttgccg gtgcccggat tgccaccgac cagcgccaac cggaccgtag cgtgctgtag gtgttgggtg gcgatgatca ggtggcgcac ggcgtccgca gcggcctccg gtttgccctg ggagaatcgc acgcactcga ctttcgcgcg caccaccgcg cgataagcaa tgtagaagtc gcgcagcgac gccggggcgg tatcacccga acgcaccgca tagccggcca ggaagtagtc cccaagatct ttgcggccca agaactccag atccatggcc aaaaaggcgg cgtcgtcgat gcggtcgagg tagcgaagct cgtcttcgaa ctccaagcaa tccagcagcg ccggttcgcc atccaccaag aagatgtcat cggccagtag atccgcgtgg ccgtctacaa tacaaccttc tttgatccgg ccggcgaaca aaacctcgcg cccggaaacg aattcgtcga ccatgtgttc aatccgccga atcacatccc cggagaccac tttgtccgcg tggtggcgaa gttcggccag gttttcgtgc caacgccgcg ccaccgcacc gacctcgcct tgagtatcga tgcaccggtt acgctgtgcg cgctggtgaa accgggccaa cacctcagcg atcgcgtcca gggcaccctc gaccggcagg ccggcggtca ccatcgacgc cagccgctgc ttgtcgcggt aacgccgcat gacgacgacc ggttcggcgt gcccgccgct tggatcgctg agatgggcaa tgcccaagta gctctgcgcg gccagccgac tattcaactc gaattcccgg atacaggcgc gctcacgctg ttccgccgtg cggaagtcgc agaaatccgt caccacaggc tttttcgcct tgaacgcccg gtcgccggcc aacacaacca ctgcggtgtg ggtttcgcgc acatcgatga aaggctcatc tgtcacagga tgggcgtcac acgtgccgtc gttggtcggt gagtc
Expressed Protein Sequence
MAHHHHHHMD SPTNDGTCDA HPVTDEPFID VRETHTAVVV LAGDRAFKAK KPVVTDFCDF RTAEQRERAC IREFELNSRL AAQSYLGIAH LSDPSGGHAE PVVVMRRYRD KQRLASMVTA GLPVEGALDA IAEVLARFHQ RAQRNRCIDT QGEVGAVARR WHENLAELRH HADKVVSGDV IRRIEHMVDE FVSGREVLFA GRIKEGCIVD GHADLLADDI FLVDGEPALL DCLEFEDELR YLDRIDDAAF LAMDLEFLGR KDLGDYFLAG YAVRSGDTAP ASLRDFYIAY RAVVRAKVEC VRFSQGKPEA AADAVRHLII ATQHLQHATV RLALVGGNPG TGKSTLARGV AELVGAQVIS TDDVRRRLRD CGVITGEPGV LDSGLYSRAN VVAVYQEALR KARLLLGSGH SVILDGTWGD PQMRACARRL AADTHSAIVE FRCSATVDVM ADRIVARAGG NSDATAEIAA ALAARQADWD TGHRIDTAGP RERSVGQAYH IWRSAI
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGACT CACCGACCAA CGACGGCACG TGTGACGCCC ATCCTGTGAC AGATGAGCCT TTCATCGATG TGCGCGAAAC CCACACCGCA GTGGTTGTGT TGGCCGGCGA CCGGGCGTTC AAGGCGAAAA AGCCTGTGGT GACGGATTTC TGCGACTTCC GCACGGCGGA ACAGCGTGAG CGCGCCTGTA TCCGGGAATT CGAGTTGAAT AGTCGGCTGG CCGCGCAGAG CTACTTGGGC ATTGCCCATC TCAGCGATCC AAGCGGCGGG CACGCCGAAC CGGTCGTCGT CATGCGGCGT TACCGCGACA AGCAGCGGCT GGCGTCGATG GTGACCGCCG GCCTGCCGGT CGAGGGTGCC CTGGACGCGA TCGCTGAGGT GTTGGCCCGG TTTCACCAGC GCGCACAGCG TAACCGGTGC ATCGATACTC AAGGCGAGGT CGGTGCGGTG GCGCGGCGTT GGCACGAAAA CCTGGCCGAA CTTCGCCACC ACGCGGACAA AGTGGTCTCC GGGGATGTGA TTCGGCGGAT TGAACACATG GTCGACGAAT TCGTTTCCGG GCGCGAGGTT TTGTTCGCCG GCCGGATCAA AGAAGGTTGT ATTGTAGACG GCCACGCGGA TCTACTGGCC GATGACATCT TCTTGGTGGA TGGCGAACCG GCGCTGCTGG ATTGCTTGGA GTTCGAAGAC GAGCTTCGCT ACCTCGACCG CATCGACGAC GCCGCCTTTT TGGCCATGGA TCTGGAGTTC TTGGGCCGCA AAGATCTTGG GGACTACTTC CTGGCCGGCT ATGCGGTGCG TTCGGGTGAT ACCGCCCCGG CGTCGCTGCG CGACTTCTAC ATTGCTTATC GCGCGGTGGT GCGCGCGAAA GTCGAGTGCG TGCGATTCTC CCAGGGCAAA CCGGAGGCCG CTGCGGACGC CGTGCGCCAC CTGATCATCG CCACCCAACA CCTACAGCAC GCTACGGTCC GGTTGGCGCT GGTCGGTGGC AATCCGGGCA CCGGCAAGTC GACCCTGGCC CGCGGGGTCG CCGAACTGGT GGGGGCGCAG GTGATCTCAA CCGACGATGT GCGCCGGCGG CTACGCGATT GCGGAGTAAT CACCGGGGAG CCCGGCGTTT TGGACTCCGG GCTCTACAGT CGTGCCAACG TTGTGGCGGT CTACCAGGAG GCGCTGCGCA AAGCCCGCCT GCTCTTGGGT AGTGGGCACT CAGTGATCCT CGACGGTACC TGGGGTGATC CGCAGATGCG CGCCTGCGCC CGGCGTCTCG CGGCGGACAC GCACTCGGCG ATTGTCGAAT TCAGGTGTTC GGCGACGGTT GACGTGATGG CCGACAGAAT CGTTGCTAGA GCCGGGGGCA ACTCCGACGC CACCGCCGAG ATCGCGGCGG CCTTGGCCGC CCGGCAAGCG GACTGGGACA CCGGGCACCG GATCGACACG GCGGGGCCGC GTGAGCGCTC CGTGGGACAG GCCTACCACA TCTGGCGCAG CGCGATCTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT