MytuD.18384.a

ESX-1 secretion system protein EccA1 (ESX conserved component A1) (Type VII secretion system protein EccA1) (T7SS protein EccA1)

CENTER ID: MytuD.18384.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18384.a.A1.GE34397 full length 1 573
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.4313
RefSeq: NP_218385.1
TubercuList: Rv3868
MTB Network Portal: Rv3868
UniProt: P9WPH9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDRLASLFE SAVSMLPMSE ARSLDLFTEI TNYDESACDA WIGRIRCGDT DRVTLFRAWY SRRNFGQLSG SVQISMSTLN ARIAIGGLYG DITYPVTSPL AITMGFAACE AAQGNYADAM EALEAAPVAG SEHLVAWMKA VVYGAAERWT DVIDQVKSAG KWPDKFLAGA AGVAHGVAAA NLALFTEAER RLTEANDSPA GEACARAIAW YLAMARRSQG NESAAVALLE WLQTTHPEPK VAAALKDPSY RLKTTTAEQI ASRADPWDPG SVVTDNSGRE RLLAEAQAEL DRQIGLTRVK NQIERYRAAT LMARVRAAKG MKVAQPSKHM IFTGPPGTGK TTIARVVANI LAGLGVIAEP KLVETSRKDF VAEYEGQSAV KTAKTIDQAL GGVLFIDEAY ALVQERDGRT DPFGQEALDT LLARMENDRD RLVVIIAGYS SDIDRLLETN EGLRSRFATR IEFDTYSPEE LLEIANVIAA ADDSALTAEA AENFLQAAKQ LEQRMLRGRR ALDVAGNGRY ARQLVEASEQ CRDMRLAQVL DIDTLDEDRL REINGSDMAE AIAAVHAHLN MRE
NT Sequence
atgactgatc gcttggccag tctgttcgaa agcgccgtca gcatgttgcc gatgtcggag gcgcggtcgc tagatctgtt caccgagatc accaactacg acgaatccgc ttgcgacgca tggatcggcc ggatccggtg tggggacacc gaccgggtga cgctgtttcg cgcctggtat tcgcgccgca atttcggaca gttgtcggga tcggtccaga tctcgatgag cacgttaaac gccaggattg ccatcggggg gctgtacggc gatatcacct acccggtcac ctcgccgcta gcgatcacca tgggctttgc cgcatgcgag gcagcgcaag gcaattacgc cgacgccatg gaggccttag aggccgcccc ggtcgcgggt tccgagcacc tggtggcgtg gatgaaggcg gttgtctacg gcgcggccga acgctggacc gacgtgatcg accaggtcaa gagtgctggg aaatggccgg acaagttttt ggccggcgcg gccggtgtgg cgcacggggt tgccgcggca aacctggcct tgttcaccga agccgaacgc cgactcaccg aggccaacga ctcgcccgcc ggtgaggcgt gtgcgcgcgc catcgcctgg tatctggcga tggcacggcg cagccagggc aacgaaagcg ccgcggtggc gctgctggaa tggttacaga ccactcaccc cgagcccaaa gtggctgcgg cgctgaagga tccctcctac cggctgaaga cgaccaccgc cgaacagatc gcatcccgcg ccgatccctg ggatccgggc agtgtcgtga ccgacaactc cggccgggag cggctgctcg ccgaggccca agccgaactc gaccgccaaa ttgggctcac ccgggttaaa aatcagattg aacgctaccg cgcggcgacg ctgatggccc gggtccgcgc cgccaagggt atgaaggtcg cccagcccag caagcacatg atcttcaccg gaccgcccgg taccggcaag accacgatcg cgcgggtggt ggccaatatc ctggccggct taggcgtcat tgccgaaccc aaactcgtcg agacgtcgcg caaggacttc gtcgccgagt acgaggggca atcggcggtc aagaccgcta agacgatcga tcaggcgctg ggcggggtgc ttttcatcga cgaggcttat gcgctggtgc aggaaagaga cggccgcacc gatccgttcg gtcaagaggc gctggacacg ctgctggcgc ggatggagaa cgaccgggac cggctggtgg tgatcatcgc cgggtacagc tccgacatag atcggctgct ggaaaccaac gagggtctgc ggtcgcggtt cgccactcgc atcgagttcg acacctattc ccccgaggaa ctcctcgaga tcgccaacgt cattgccgct gctgatgatt cggcgttgac cgcagaggcg gccgagaact ttcttcaggc cgccaagcag ttggagcagc gcatgttgcg cggccggcgc gccctggacg tcgccggcaa cggtcggtat gcgcgccagc tggtggaggc cagcgagcaa tgccgggaca tgcgtctagc ccaggtcctc gatatcgaca ccctcgacga agaccggctt cgcgagatca acggctcaga tatggcggag gctatcgccg cggtgcacgc acacctcaac atgagagaat ga
Details for MytuD.18384.a.A1.GE34397
HARVESTED ON: 1/10/2012
SEQUENCED ON: 1/25/2012
EXPECTED MW: 64kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 92
PERCENT COVERAGE: 44
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTDRLASLF ESAVSMLPMS EARSLDLFTE ITNYDESACD AWIGRIRCGD TDRVTLFRAW YSRRNFGQLS GSVQISMSTL NARIAIGGLX XDITYPVTSP LAITMGFAAC EAAQGNYADA MEALEAAPVA GSEHLVAWMK AVVYGAAERW TDVIDQVKSA GKWPDKFLAG AAGVAHGVAA ANLALFTEAE RRLTEANDSP AGEACARAIA XXLAMXRRXX GNESAAVALL NGYRPLTPSX XWLRR
Validated NT Sequence
tannnatttt nnttanttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gactgatcgc ttggccagtc tgttcgaaag cgccgtcagc atgttgccga tgtcggaggc gcggtcgcta gatctgttca ccgagatcac caactacgac gaatccgctt gcgacgcatg gatcggccgg atccggtgtg gggacaccga ccgggtgacg ctgtttcgcg cctggtattc gcgccgcaat ttcggacagt tgtcgggatc ggtccagatc tcgatgagca cgttaaacgc caggattgcc atcggggggc tgtnnngnga tatcacctac ccggtcacct cgccgctagc gatcaccatg ggctttgccg catgcgaggc agcgcaaggc aattacgccg acgccatgga ggccttagag gccgccccgg tcgcgggttc cgagcacctg gtggcgtgga tgaaggcggt tgtctacggc gcggccgaac gctggaccga cgtgatcgac caggtcaaga gtgctgggaa atggccggac aagtttttgg ccggcgcggc cggtgtggcg cacggggttg ccgcggcaaa cctggccttg ttcaccgaag ccgaacgccg actcaccgag gccaacgact cgcccgccgg tgaggcgtgt gcgcgcgcca tcgcctgnna tctggcgatg gnacggcgca nccngggcaa cgaaagcgcc gcggtggcgc tgctgaatgg ttacagacca ctcaccccga gcncaanntg gctgcggcgc tganatccct cctaccggct gaagacgacc accgccgaac agatcgcatc ccgcgccgat ccctggganc cnggcagngt cgtgaccgac aactnnngcc gggagcggct gntcgccnag gcccnagccn aactcgaccg ccnaattggg ntnccnnggn naaaaannca natnnannct nnnnnnnnng gngnannnct tnnnnncccg ggnnnnnnnn nncc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDRLASLF ESAVSMLPMS EARSLDLFTE ITNYDESACD AWIGRIRCGD TDRVTLFRAW YSRRNFGQLS GSVQISMSTL NARIAIGGLY GDITYPVTSP LAITMGFAAC EAAQGNYADA MEALEAAPVA GSEHLVAWMK AVVYGAAERW TDVIDQVKSA GKWPDKFLAG AAGVAHGVAA ANLALFTEAE RRLTEANDSP AGEACARAIA WYLAMARRSQ GNESAAVALL EWLQTTHPEP KVAAALKDPS YRLKTTTAEQ IASRADPWDP GSVVTDNSGR ERLLAEAQAE LDRQIGLTRV KNQIERYRAA TLMARVRAAK GMKVAQPSKH MIFTGPPGTG KTTIARVVAN ILAGLGVIAE PKLVETSRKD FVAEYEGQSA VKTAKTIDQA LGGVLFIDEA YALVQERDGR TDPFGQEALD TLLARMENDR DRLVVIIAGY SSDIDRLLET NEGLRSRFAT RIEFDTYSPE ELLEIANVIA AADDSALTAE AAENFLQAAK QLEQRMLRGR RALDVAGNGR YARQLVEASE QCRDMRLAQV LDIDTLDEDR LREINGSDMA EAIAAVHAHL NMRE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gactgatcgc ttggccagtc tgttcgaaag cgccgtcagc atgttgccga tgtcggaggc gcggtcgcta gatctgttca ccgagatcac caactacgac gaatccgctt gcgacgcatg gatcggccgg atccggtgtg gggacaccga ccgggtgacg ctgtttcgcg cctggtattc gcgccgcaat ttcggacagt tgtcgggatc ggtccagatc tcgatgagca cgttaaacgc caggattgcc atcggggggc tgtacggcga tatcacctac ccggtcacct cgccgctagc gatcaccatg ggctttgccg catgcgaggc agcgcaaggc aattacgccg acgccatgga ggccttagag gccgccccgg tcgcgggttc cgagcacctg gtggcgtgga tgaaggcggt tgtctacggc gcggccgaac gctggaccga cgtgatcgac caggtcaaga gtgctgggaa atggccggac aagtttttgg ccggcgcggc cggtgtggcg cacggggttg ccgcggcaaa cctggccttg ttcaccgaag ccgaacgccg actcaccgag gccaacgact cgcccgccgg tgaggcgtgt gcgcgcgcca tcgcctggta tctggcgatg gcacggcgca gccagggcaa cgaaagcgcc gcggtggcgc tgctggaatg gttacagacc actcaccccg agcccaaagt ggctgcggcg ctgaaggatc cctcctaccg gctgaagacg accaccgccg aacagatcgc atcccgcgcc gatccctggg atccgggcag tgtcgtgacc gacaactccg gccgggagcg gctgctcgcc gaggcccaag ccgaactcga ccgccaaatt gggctcaccc gggttaaaaa tcagattgaa cgctaccgcg cggcgacgct gatggcccgg gtccgcgccg ccaagggtat gaaggtcgcc cagcccagca agcacatgat cttcaccgga ccgcccggta ccggcaagac cacgatcgcg cgggtggtgg ccaatatcct ggccggctta ggcgtcattg ccgaacccaa actcgtcgag acgtcgcgca aggacttcgt cgccgagtac gaggggcaat cggcggtcaa gaccgctaag acgatcgatc aggcgctggg cggggtgctt ttcatcgacg aggcttatgc gctggtgcag gaaagagacg gccgcaccga tccgttcggt caagaggcgc tggacacgct gctggcgcgg atggagaacg accgggaccg gctggtggtg atcatcgccg ggtacagctc cgacatagat cggctgctgg aaaccaacga gggtctgcgg tcgcggttcg ccactcgcat cgagttcgac acctattccc ccgaggaact cctcgagatc gccaacgtca ttgccgctgc tgatgattcg gcgttgaccg cagaggcggc cgagaacttt cttcaggccg ccaagcagtt ggagcagcgc atgttgcgcg gccggcgcgc cctggacgtc gccggcaacg gtcggtatgc gcgccagctg gtggaggcca gcgagcaatg ccgggacatg cgtctagccc aggtcctcga tatcgacacc ctcgacgaag accggcttcg cgagatcaac ggctcagata tggcggaggc tatcgccgcg gtgcacgcac acctcaacat gagagaataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa