MytuD.18324.a

CONSERVED HYPOTHETICAL PROTEIN

CENTER ID: MytuD.18324.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18324.a.A1.GE34226 full length 1 380
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.637
RefSeq: NP_215088
TubercuList: Rv0574c
MTB Network Portal: Rv0574c
UniProt: P9WM79

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAGNPDVVTV LLGGDVMLGR GVDQILPHPG KPQLRERYMR DATGYVRLAE RVNGRIPLPV DWRWPWGEAL AVLENTATDV CLINLETTIT ADGEFADRKP VCYRMHPDNV PALTALRPHV CALANNHILD FGYQGLTDTV AALAGAGIQS VGAGADLLAA RRSALVTVGH ERRVIVGSVA AESSGVPESW AARRDRPGVW LIRDPAQRDV ADDVAAQVLA DKRPGDIAIV SMHWGSNWGY ATAPGDVAFA HRLIDAGIDM VHGHSSHHPR PIEIYRGKPI LYGCGDVVDD YEGIGGHESF RSELRLLYLT VTDPASGNLI SLQMLPLRVS RMRLQRASQT DTEWLRNTIE RISRRFGIRV VTRPDNLLEV VPAANLTSKE
NT Sequence
gtggctggca atcctgatgt ggtgacggtg ctgctgggcg gtgacgtcat gctcggccgt ggcgtcgatc agatcctgcc tcatcccggc aaaccgcaat tgcgcgaacg gtatatgcgg gatgcgaccg gctatgttcg cctggccgag cgggtgaacg ggcgcattcc gctccccgtg gattggcgct ggccctgggg cgaggcgttg gcggtccttg agaacaccgc gaccgacgtc tgtttgatca atctggagac gacgatcacc gccgacggtg aattcgccga ccgcaaaccg gtctgctacc ggatgcaccc ggataacgtg ccggcgctga cggcattgcg gccgcacgtg tgcgcgctgg ccaacaacca cattctcgat ttcggctacc aggggctgac cgatacggtc gcggctctcg ccggtgcggg gatccagagt gtcggggcgg gagccgattt gctcgccgct cgccgctcgg cgctagtcac ggttggccat gaacgccggg tgatcgtcgg ctcggtagcg gcggaatcca gcggcgtccc cgaatcctgg gccgcccgcc gcgaccggcc cggagtgtgg ttgatccggg atccggcgca acgcgacgtc gccgacgatg tggcggcaca ggtgctggcg gacaaacgcc ccggcgatat cgccatagtc tcgatgcatt ggggatccaa ttggggctat gcgaccgcac ccggcgacgt cgcgttcgcg caccgactga tcgacgccgg catcgacatg gtccacggac attcctcgca ccatccgcgg ccaatcgaga tatatcgcgg taaaccgatc ctgtacggat gcggtgacgt cgttgacgac tacgaaggca tcggcgggca cgagtcgttc cgcagtgaac tgcgactgct gtatctgacc gtcaccgatc ccgccagcgg gaacctgatc tcgctgcaga tgcttccact gcgagtgtcg cggatgcgcc tacagcgtgc ctcccagacc gacaccgaat ggctccgcaa caccattgag cgcatcagcc gccggttcgg gattcgagtc gtgactcgac ccgacaacct gctggaggtc gttcccgctg ccaacctaac gagcaaggag taa
Details for MytuD.18324.a.A1.GE34226
HARVESTED ON: 12/19/2011
SEQUENCED ON: 12/28/2011
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 74
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVAGNPDVV TVLLGGDVML GRGVDQILPH PGKPQLRERY MRDATGYVRL AERVNGRIPL PVDWRWPWGE ALAVLENTAT DVCLINLETT ITADGEFADR KPVCYRMHPD NVPALTALRP HVCALANNHI LDFGYQGLTD TVAALAGAGI QSVGAGADLL AARRSALVTV GHERRVIVGS VAAESSGVPE SWAARRDRPG VWLIRXPAQR DVADDVAAQV LADKRPGDIA IVSMHWGSNW GYATAPGDVA FAHRLIDAGX DMVHGHSSHH PXAXRDISR
Validated NT Sequence
attttnntta ctttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgg ctggcaatcc tgatgtggtg acggtgctgc tgggcggtga cgtcatgctc ggccgtggcg tcgatcagat cctgcctcat cccggcaaac cgcaattgcg cgaacggtat atgcgggatg cgaccggcta tgttcgcctg gccgagcggg tgaacgggcg cattccgctc cccgtggatt ggcgctggcc ctggggcgag gcgttggcgg tccttgagaa caccgcgacc gacgtctgtt tgatcaatct ggagacgacg atcaccgccg acggtgaatt cgccgaccgc aaaccggtct gctaccggat gcacccggat aacgtgccgg cgctgacggc attgcggccg cacgtgtgcg cgctggccaa caaccacatt ctcgatttcg gctaccaggg gctgaccgat acggtcgcgg ctctcgccgg tgcggggatc cagagtgtcg gggcgggagc cgatttgctc gccgctcgcc gctcggcgct agtcacggtt ggccatgaac gccgggtgat cgtcggctcg gtagcggcgg aatccagcgg cgtccccgaa tcctgggccg cccgccgcga ccggcccgga gtgtggttga tccgggancc ggcgcaacgc gacgtcgccg acgatgtggc ggcacaggtg ctggcggaca aacgccccgg cgatatcgcc atagtctcga tgcattgggg atccaattgg ggctatgcga ccgcacccgg cgacgtcgcg ttcgcgcacc gactgatcga cgccggcntc gacatggtcc acggacattc ctcgcaccat ccgcnggccn atcgagatat atcgcggtaa accgatcntg tacgnatgcn nngacgtcgt tgacgactac naagncntcg gcgggnacna gtcntnccnc antgnactnn nactgctnnn tctgacncga tncnnnnngc ngnnnnnatn nnnctgcnna tgnnnnnctn nnnnnngcnn nncngnnnnn nnncnnannn nnnnnnnnnn ccnnnacnnn nnnnnntnnn nnnnnnnnnn nnnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVAGNPDVV TVLLGGDVML GRGVDQILPH PGKPQLRERY MRDATGYVRL AERVNGRIPL PVDWRWPWGE ALAVLENTAT DVCLINLETT ITADGEFADR KPVCYRMHPD NVPALTALRP HVCALANNHI LDFGYQGLTD TVAALAGAGI QSVGAGADLL AARRSALVTV GHERRVIVGS VAAESSGVPE SWAARRDRPG VWLIRDPAQR DVADDVAAQV LADKRPGDIA IVSMHWGSNW GYATAPGDVA FAHRLIDAGI DMVHGHSSHH PRPIEIYRGK PILYGCGDVV DDYEGIGGHE SFRSELRLLY LTVTDPASGN LISLQMLPLR VSRMRLQRAS QTDTEWLRNT IERISRRFGI RVVTRPDNLL EVVPAANLTS KE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggctggc aatcctgatg tggtgacggt gctgctgggc ggtgacgtca tgctcggccg tggcgtcgat cagatcctgc ctcatcccgg caaaccgcaa ttgcgcgaac ggtatatgcg ggatgcgacc ggctatgttc gcctggccga gcgggtgaac gggcgcattc cgctccccgt ggattggcgc tggccctggg gcgaggcgtt ggcggtcctt gagaacaccg cgaccgacgt ctgtttgatc aatctggaga cgacgatcac cgccgacggt gaattcgccg accgcaaacc ggtctgctac cggatgcacc cggataacgt gccggcgctg acggcattgc ggccgcacgt gtgcgcgctg gccaacaacc acattctcga tttcggctac caggggctga ccgatacggt cgcggctctc gccggtgcgg ggatccagag tgtcggggcg ggagccgatt tgctcgccgc tcgccgctcg gcgctagtca cggttggcca tgaacgccgg gtgatcgtcg gctcggtagc ggcggaatcc agcggcgtcc ccgaatcctg ggccgcccgc cgcgaccggc ccggagtgtg gttgatccgg gatccggcgc aacgcgacgt cgccgacgat gtggcggcac aggtgctggc ggacaaacgc cccggcgata tcgccatagt ctcgatgcat tggggatcca attggggcta tgcgaccgca cccggcgacg tcgcgttcgc gcaccgactg atcgacgccg gcatcgacat ggtccacgga cattcctcgc accatccgcg gccaatcgag atatatcgcg gtaaaccgat cctgtacgga tgcggtgacg tcgttgacga ctacgaaggc atcggcgggc acgagtcgtt ccgcagtgaa ctgcgactgc tgtatctgac cgtcaccgat cccgccagcg ggaacctgat ctcgctgcag atgcttccac tgcgagtgtc gcggatgcgc ctacagcgtg cctcccagac cgacaccgaa tggctccgca acaccattga gcgcatcagc cgccggttcg ggattcgagt cgtgactcga cccgacaacc tgctggaggt cgttcccgct gccaacctaa cgagcaagga gtaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa