SSGCID: Target Page

MytuD.18320.a

ADENYLYL CYCLASE (ATP PYROPHOSPHATE-LYASE) (ADENYLATE CYCLASE)

CENTER ID:	MytuD.18320.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.18320.a.A1.GE34237	full length		1	378

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.18320.a.A1.PW34845	full length		1	378

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_171684
PATRIC ID:	fig\|83332.12.peg.2464
RefSeq:	NP_216728
TubercuList:	Rv2212
MTB Network Portal:	Rv2212
UniProt:	P9WMU7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MYDSLDFDAL EAAGIANPRE RAGLLTYLDE LGFTVEEMVQ AERRGRLFGL AGDVLLWSGP PIYTLATAAD ELGLSADDVA RAWSLLGLTV AGPDVPTLSQ ADVDALATWV ALKALVGEDG AFGLLRVLGT AMARLAEAES TMIRAGSPNI QMTHTHDELA TARAYRAAAE FVPRIGALID TVHRHHLASA RTYFEGVIGD TSASVTCGIG FADLSSFTAL TQALTPAQLQ DLLTEFDAAV TDVVHADGGR LVKFIGDAVM WVSSSPERLV RAAVDLVDHP GARAAELQVR AGLAYGTVLA LNGDYFGNPV NLAARLVAAA APGQILAAAQ LRDMLPDWPA LAHGPLTLKG FDAPVMAFEL HDNPRARDAD TPSPAASD

NT Sequence

gtgtacgatt ccttggactt cgacgccctc gaggccgccg gaattgccaa cccacgcgag cgggccggct tgctcaccta cctggatgag cttggcttca cggtcgaaga gatggtgcaa gccgaacgcc gcggccggtt gttcgggctg gccggtgacg tcctgctatg gtccgggccc ccgatctaca ccctggcgac cgcggctgac gaactggggt tgtcagccga cgacgtcgca cgcgcgtgga gtttgctcgg cctcaccgtc gcgggtcccg acgttcccac gctgagccag gccgacgtcg acgccctggc gacctgggtc gcactgaagg cgctggtggg tgaggacggc gcattcggcc tgctgcgagt gctcggcact gccatggccc gactcgccga ggccgagtcg accatgatcc gcgccgggtc accgaacatc caaatgacgc acacccacga cgaacttgcc acggcacggg cctatcgcgc ggctgcggag ttcgtccccc ggatcggtgc gctgatcgac accgtccacc gtcaccacct ggccagcgca cgaacctact ttgaaggcgt cattggcgac acgtcggcaa gcgtgacgtg cggtatcggc tttgcggatc tgtccagctt caccgcgttg acccaggcgc tcacccccgc gcagttgcag gacctgctca ccgaattcga cgccgccgtc accgacgtgg tgcatgccga cggtggccgg ttggtgaagt tcatcggcga cgccgtgatg tgggtgagct cgtcgcccga acgactggtg cgggcggcgg tggatctcgt cgatcatccg ggtgcgcgcg cggccgaact gcaggtccgt gccggtcttg cctatggcac ggtgctggcc cttaacggtg actacttcgg caacccggtc aacctggctg cgcgcctggt ggcggccgca gcgccagggc agatcctggc cgcagcgcaa ctccgcgaca tgttgccaga ctggcctgcc ctcgcccatg gcccattgac gctcaagggg tttgacgccc cggtgatggc cttcgaactg cacgacaacc ctcgtgcgag ggatgctgac acgccaagcc ccgccgccag tgattag

Details for MytuD.18320.a.A1.GE34237

HARVESTED ON:	12/19/2011
SEQUENCED ON:	1/3/2012
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	24
PERCENT COVERAGE:	63

Validated AA Sequence

MGEGRPVWQH VAELRCGQDL PWRCGRHQAR SQVDRVAEVV TVKGQHRAIG KTGTDLQFGR ARTRMIDEIH RRPHQSFGRR AHPHHGVADE LHQPATVGMH HVGDGGVEFG EQVLQLRGGE RLGQRGEAGQ IRKADTARHA CRRVANDAFK VGSCAGXGGD GGRCRSAHRS GGRTPQPRDR PVPWQVRRGC ASFGCSVTRR GSWSTRPRRV GPWQCRALAA GRMRRPHPPA PSVRPRSPGR RRRPGSAWER RDPRR

Validated NT Sequence

actggcggcg gggcttggcg tgtcagcatc cctcgcacga gggttgtcgt gcagttcgaa ggccatcacc ggggcgtcaa accccttgag cgtcaatggg ccatgggcga gggcaggcca gtctggcaac atgtcgcgga gttgcgctgc ggccaggatc tgccctggcg ctgcggccgc caccaggcgc gcagccaggt tgaccgggtt gccgaagtag tcaccgttaa gggccagcac cgtgccatag gcaagaccgg cacggacctg cagttcggcc gcgcgcgcac ccggatgatc gacgagatcc accgccgccc gcaccagtcg ttcgggcgac gagctcaccc acatcacggc gtcgccgatg aacttcacca accggccacc gtcggcatgc accacgtcgg tgacggcggc gtcgaattcg gtgagcaggt cctgcaactg cgcgggggtg agcgcctggg tcaacgcggt gaagctggac agatccgcaa agccgatacc gcacgtcacg cttgccgacg tgtcgcnaat gacgccttca aagtaggttc gtgcgctggc cnaggtggtg acggtggacg gtgtcgatca gcgcaccgat ccgggggacg aactccgcag ccgcgcgata ggcccgtgcc gtggcaagtt cgtcgtgggt gtgcgtcatt tggatgttcg gtgacccggc gcggatcatg gtcgactcgg cctcggcgag tcgggccatg gcagtgccga gcactcgcag caggccgaat gcgccgtcct cacccaccag cgccttcagt gcgacccagg tcgccagggc gtcgacgtcg ncctggctca gcgtgggaac gtcgggaccc gcgacggtga ggccgagcaa actccacgcg cgtgcgacgt cgtcggctga caaccccagt tcgtcagccg cggtcgccag ggtgtagatc gggggcccgg accatagcag gacgtcaccg gccagcccga acaaccggcc gcggcgttcg gcttgcacca tctcttcgac cgtgaagcca agctcatcca ggtaggtgag caagccggcc cgctcgcgtg ggttggcaat tccggcggcc tcgagggcgt cgaagtccaa ggaatcgtac accatcgaac caggaccctg ggtctgagct tccagg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVYDSLDFD ALEAAGIANP RERAGLLTYL DELGFTVEEM VQAERRGRLF GLAGDVLLWS GPPIYTLATA ADELGLSADD VARAWSLLGL TVAGPDVPTL SQADVDALAT WVALKALVGE DGAFGLLRVL GTAMARLAEA ESTMIRAGSP NIQMTHTHDE LATARAYRAA AEFVPRIGAL IDTVHRHHLA SARTYFEGVI GDTSASVTCG IGFADLSSFT ALTQALTPAQ LQDLLTEFDA AVTDVVHADG GRLVKFIGDA VMWVSSSPER LVRAAVDLVD HPGARAAELQ VRAGLAYGTV LALNGDYFGN PVNLAARLVA AAAPGQILAA AQLRDMLPDW PALAHGPLTL KGFDAPVMAF ELHDNPRARD ADTPSPAASD

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtacgat tccttggact tcgacgccct cgaggccgcc ggaattgcca acccacgcga gcgggccggc ttgctcacct acctggatga gcttggcttc acggtcgaag agatggtgca agccgaacgc cgcggccggt tgttcgggct ggccggtgac gtcctgctat ggtccgggcc cccgatctac accctggcga ccgcggctga cgaactgggg ttgtcagccg acgacgtcgc acgcgcgtgg agtttgctcg gcctcaccgt cgcgggtccc gacgttccca cgctgagcca ggccgacgtc gacgccctgg cgacctgggt cgcactgaag gcgctggtgg gtgaggacgg cgcattcggc ctgctgcgag tgctcggcac tgccatggcc cgactcgccg aggccgagtc gaccatgatc cgcgccgggt caccgaacat ccaaatgacg cacacccacg acgaacttgc cacggcacgg gcctatcgcg cggctgcgga gttcgtcccc cggatcggtg cgctgatcga caccgtccac cgtcaccacc tggccagcgc acgaacctac tttgaaggcg tcattggcga cacgtcggca agcgtgacgt gcggtatcgg ctttgcggat ctgtccagct tcaccgcgtt gacccaggcg ctcacccccg cgcagttgca ggacctgctc accgaattcg acgccgccgt caccgacgtg gtgcatgccg acggtggccg gttggtgaag ttcatcggcg acgccgtgat gtgggtgagc tcgtcgcccg aacgactggt gcgggcggcg gtggatctcg tcgatcatcc gggtgcgcgc gcggccgaac tgcaggtccg tgccggtctt gcctatggca cggtgctggc ccttaacggt gactacttcg gcaacccggt caacctggct gcgcgcctgg tggcggccgc agcgccaggg cagatcctgg ccgcagcgca actccgcgac atgttgccag actggcctgc cctcgcccat ggcccattga cgctcaaggg gtttgacgcc ccggtgatgg ccttcgaact gcacgacaac cctcgtgcga gggatgctga cacgccaagc cccgccgcca gtgattaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa

Details for MytuD.18320.a.A1.PW34845

PURIFICATION DATe:	1/29/2012
CONCENTRATION:	47.99mg/ml
OBSERVED MW:	40kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	110µl
PERCENT IDENTITY:	24
PERCENT COVERAGE:	63

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGTACGAT TCCTTGGACT TCGACGCCCT CGAGGCCGCC GGAATTGCCA ACCCACGCGA GCGGGCCGGC TTGCTCACCT ACCTGGATGA GCTTGGCTTC ACGGTCGAAG AGATGGTGCA AGCCGAACGC CGCGGCCGGT TGTTCGGGCT GGCCGGTGAC GTCCTGCTAT GGTCCGGGCC CCCGATCTAC ACCCTGGCGA CCGCGGCTGA CGAACTGGGG TTGTCAGCCG ACGACGTCGC ACGCGCGTGG AGTTTGCTCG GCCTCACCGT CGCGGGTCCC GACGTTCCCA CGCTGAGCCA GGCCGACGTC GACGCCCTGG CGACCTGGGT CGCACTGAAG GCGCTGGTGG GTGAGGACGG CGCATTCGGC CTGCTGCGAG TGCTCGGCAC TGCCATGGCC CGACTCGCCG AGGCCGAGTC GACCATGATC CGCGCCGGGT CACCGAACAT CCAAATGACG CACACCCACG ACGAACTTGC CACGGCACGG GCCTATCGCG CGGCTGCGGA GTTCGTCCCC CGGATCGGTG CGCTGATCGA CACCGTCCAC CGTCACCACC TGGCCAGCGC ACGAACCTAC TTTGAAGGCG TCATTGGCGA CACGTCGGCA AGCGTGACGT GCGGTATCGG CTTTGCGGAT CTGTCCAGCT TCACCGCGTT GACCCAGGCG CTCACCCCCG CGCAGTTGCA GGACCTGCTC ACCGAATTCG ACGCCGCCGT CACCGACGTG GTGCATGCCG ACGGTGGCCG GTTGGTGAAG TTCATCGGCG ACGCCGTGAT GTGGGTGAGC TCGTCGCCCG AACGACTGGT GCGGGCGGCG GTGGATCTCG TCGATCATCC GGGTGCGCGC GCGGCCGAAC TGCAGGTCCG TGCCGGTCTT GCCTATGGCA CGGTGCTGGC CCTTAACGGT GACTACTTCG GCAACCCGGT CAACCTGGCT GCGCGCCTGG TGGCGGCCGC AGCGCCAGGG CAGATCCTGG CCGCAGCGCA ACTCCGCGAC ATGTTGCCAG ACTGGCCTGC CCTCGCCCAT GGCCCATTGA CGCTCAAGGG GTTTGACGCC CCGGTGATGG CCTTCGAACT GCACGACAAC CCTCGTGCGA GGGATGCTGA CACGCCAAGC CCCGCCGCCA GTGATTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA