MytuD.18311.a

PE-PGRS FAMILY PROTEIN

CENTER ID: MytuD.18311.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18311.a.A2.GE34238 mature protein 32 515
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.2052
RefSeq: YP_177847
TubercuList: Rv1840c
MTB Network Portal: Rv1840c
UniProt: P9WIF3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSFVVAAPEV VVAAASDLAG IGSAIGAANA AAAVPTMGVL AAGADEVSAA VADLFGAHAQ AYQALSAQAA LFHEQFVHAM TAGAGAYAGA EAADAAALDV LNGPFQALFG RPLIGDGANG APGQPGGPGG LLYGNGGNGG NGGIGQPGGA GGDAGLIGNG GNGGIGGPGA TGLAGGAGGV GGLLFGDGGN GGAGGLGTGP VGATGGIGGP GGAAVGLFGH GGAGGAGGLG KAGFAGGAGG TGGTGGLLYG NGGNGGNVPS GAADGGAGGD ARLIGNGGDG GSVGAAPTGI GNGGNGGNGG WLYGDGGSGG STLQGFSDGG TGGNAGMFGD GGNGGFSFFD GNGGDGGTGG TLIGNGGDGG NSVQTDGFLR GHGGDGGNAV GLIGNGGAGG AGSAGTGVFA PGGGSGGNGG NGALLVGNGG AGGSGGPTQI PSVAVPVTGA GGTGGNGGTA GLIGNGGNGG AAGVSGDGTP GTGGNGGYAQ LIGDGGDGGP GDSGGPGGSG GTGGTLAGQN GSPGG
NT Sequence
atgtcgtttg tggtggcggc cccagaagtg gtggtggcgg cggcgtcgga tttggccggc atcgggtcgg cgatcggcgc ggcgaatgcg gctgcggccg tgcccacgat gggggtgctg gccgcgggcg ccgatgaggt gtcggcggct gttgcggatt tgttcggcgc gcacgcccag gcctatcagg cattgagtgc gcaggcggcg ctgtttcacg agcagtttgt gcatgccatg accgccggcg cgggggccta tgccggtgcc gaggcggcgg acgccgccgc gctggacgtg ctcaacgggc cgtttcaggc gttgttcggc cgcccgttga tcggtgatgg cgccaatggc gcgccgggac agcccggcgg gcccggcggg ttgctgtacg gcaacggcgg caacggcggc aacggcggga tcggccagcc cggcggcgcc ggtggggatg cagggttgat cggcaacggc ggcaacggtg gcatcggcgg gcccggggcg accggccttg ccggtggtgc cggcggggtc ggcggactgc tcttcggcga cggcggcaac ggcggtgccg gcggactcgg taccggaccc gtcggcgcta ccggtgggat cggcgggccc ggtggagccg ccgtcgggct gttcggccat ggcggggccg gcggggccgg cgggctcggc aaggccggct tcgcgggcgg ggccggcggc accggcggca ccggcggact gctgtacggc aacggcggca acggcggcaa tgtcccatcg ggagccgccg acggcggagc gggcggtgac gcccggctga tcggcaacgg gggcgacggc ggctccgtcg gtgccgcccc caccggcatc ggcaacggcg gcaacggcgg caacggcggg tggctgtacg gcgacggcgg ttccggaggc agcaccctgc aaggtttcag cgacggtggt accggcggta acgccgggat gttcggcgac ggcggtaacg gcgggttcag cttcttcgat ggcaacggcg gcgacggcgg caccggcggg acgctgatcg gcaacggcgg cgacggcggc aacagcgtgc aaaccgacgg ctttctccga ggccacggcg gcgacggcgg caacgccgtc gggctgatcg gcaacggtgg cgccggaggc gccgggtccg ccggtaccgg cgtcttcgcc cccggcggtg gcagtggcgg gaacggaggc aacggcgcgc tgctggtcgg caacggtggc gccggcggct ccggtgggcc aacccagatt ccctcagtcg cggtacccgt caccggcgcc ggcggcaccg ggggtaacgg cggcacggcc gggttgatcg gcaacggtgg caatggcggc gccgccggcg tcagcgggga cggcaccccc ggcaccggcg gtaacggcgg ctacgcccag ttgatcggcg acggcggcga cggtgggccc ggcgactctg gcgggccggg cggcagcggc ggtacgggcg gaacgctggc cggccagaac gggtcccccg ggggatag
Details for MytuD.18311.a.A2.GE34238
HARVESTED ON: 12/19/2011
SEQUENCED ON: 1/3/2012
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 30
PERCENT COVERAGE: 13
Validated AA Sequence
MHKLLVKQRR LRTQCLIGLG VRXEQIRNSR RHLIGXRGQH PHRGHGRHRT RTLGLSFQGT HMMVMVMS
Validated NT Sequence
tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggcggc cgtgcccacg atgggggtgc tggccgcggg nnccgatgag gtgtcggcgg ctgttgcgga tttgttcggn gcgcacgccc aggcctatca ggcattgagt gcgcaggcgg cgctgtttca cgagcagttt gtgcatgcca tgaccgccgg cgc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAAVPTMGV LAAGADEVSA AVADLFGAHA QAYQALSAQA ALFHEQFVHA MTAGAGAYAG AEAADAAALD VLNGPFQALF GRPLIGDGAN GAPGQPGGPG GLLYGNGGNG GNGGIGQPGG AGGDAGLIGN GGNGGIGGPG ATGLAGGAGG VGGLLFGDGG NGGAGGLGTG PVGATGGIGG PGGAAVGLFG HGGAGGAGGL GKAGFAGGAG GTGGTGGLLY GNGGNGGNVP SGAADGGAGG DARLIGNGGD GGSVGAAPTG IGNGGNGGNG GWLYGDGGSG GSTLQGFSDG GTGGNAGMFG DGGNGGFSFF DGNGGDGGTG GTLIGNGGDG GNSVQTDGFL RGHGGDGGNA VGLIGNGGAG GAGSAGTGVF APGGGSGGNG GNGALLVGNG GAGGSGGPTQ IPSVAVPVTG AGGTGGNGGT AGLIGNGGNG GAAGVSGDGT PGTGGNGGYA QLIGDGGDGG PGDSGGPGGS GGTGGTLAGQ NGSPGG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggccgtg cccacgatgg gggtgctggc cgcgggcgcc gatgaggtgt cggcggctgt tgcggatttg ttcggcgcgc acgcccaggc ctatcaggca ttgagtgcgc aggcggcgct gtttcacgag cagtttgtgc atgccatgac cgccggcgcg ggggcctatg ccggtgccga ggcggcggac gccgccgcgc tggacgtgct caacgggccg tttcaggcgt tgttcggccg cccgttgatc ggtgatggcg ccaatggcgc gccgggacag cccggcgggc ccggcgggtt gctgtacggc aacggcggca acggcggcaa cggcgggatc ggccagcccg gcggcgccgg tggggatgca gggttgatcg gcaacggcgg caacggtggc atcggcgggc ccggggcgac cggccttgcc ggtggtgccg gcggggtcgg cggactgctc ttcggcgacg gcggcaacgg cggtgccggc ggactcggta ccggacccgt cggcgctacc ggtgggatcg gcgggcccgg tggagccgcc gtcgggctgt tcggccatgg cggggccggc ggggccggcg ggctcggcaa ggccggcttc gcgggcgggg ccggcggcac cggcggcacc ggcggactgc tgtacggcaa cggcggcaac ggcggcaatg tcccatcggg agccgccgac ggcggagcgg gcggtgacgc ccggctgatc ggcaacgggg gcgacggcgg ctccgtcggt gccgccccca ccggcatcgg caacggcggc aacggcggca acggcgggtg gctgtacggc gacggcggtt ccggaggcag caccctgcaa ggtttcagcg acggtggtac cggcggtaac gccgggatgt tcggcgacgg cggtaacggc gggttcagct tcttcgatgg caacggcggc gacggcggca ccggcgggac gctgatcggc aacggcggcg acggcggcaa cagcgtgcaa accgacggct ttctccgagg ccacggcggc gacggcggca acgccgtcgg gctgatcggc aacggtggcg ccggaggcgc cgggtccgcc ggtaccggcg tcttcgcccc cggcggtggc agtggcggga acggaggcaa cggcgcgctg ctggtcggca acggtggcgc cggcggctcc ggtgggccaa cccagattcc ctcagtcgcg gtacccgtca ccggcgccgg cggcaccggg ggtaacggcg gcacggccgg gttgatcggc aacggtggca atggcggcgc cgccggcgtc agcggggacg gcacccccgg caccggcggt aacggcggct acgcccagtt gatcggcgac ggcggcgacg gtgggcccgg cgactctggc gggccgggcg gcagcggcgg tacgggcgga acgctggccg gccagaacgg gtcccccggg ggataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa