MytuD.18275.a

PROBABLE CYTOCHROME P450 130 CYP130

CENTER ID: MytuD.18275.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18275.a.A1.GE34236 full length 1 489
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.2529
RefSeq: NP_216784
TubercuList: Rv2268c
MTB Network Portal: Rv2268c
UniProt: P9WPN7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTATQSPPEP APDRVRLAGC PLAGTPDVGL TAQDATTALG VPTRRRASSG GIPVATSMWR DAQTVRTYGP AVAKALALRV AGKARSRLTG RHCRKFMQLT DFDPFDPAIA ADPYPHYREL LAGERVQYNP KRDVYILSRY ADVREAARNH DTLSSARGVT FSRGWLPFLP TSDPPAHTRM RKQLAPGMAR GALETWRPMV DQLARELVGG LLTQTPADVV STVAAPMPMR AITSVLGVDG PDEAAFCRLS NQAVRITDVA LSASGLISLV QGFAGFRRLR ALFTHRRDNG LLRECTVLGK LATHAEQGRL SDDELFFFAV LLLVAGYEST AHMISTLFLT LADYPDQLTL LAQQPDLIPS AIEEHLRFIS PIQNICRTTR VDYSVGQAVI PAGSLVLLAW GAANRDPRQY EDPDVFRADR NPVGHLAFGS GIHLCPGTQL ARMEGQAILR EIVANIDRIE VVEPPTWTTN ANLRGLTRLR VAVTPRVAP
NT Sequence
atgaccgcga cacagtcccc tcccgagccc gcgccggacc gagtgcggct ggccgggtgc ccgttagcgg gcacgcccga cgttggtctg accgcgcagg acgcgaccac ggctctgggc gttcccacgc gccggcgcgc gtcatctggt ggcatcccgg tagccaccag catgtggcgc gacgcgcaaa ctgtgcgaac ctacgggccc gcggtcgcga aagcacttgc gctgagggtc gccgggaagg ctcggtccag gttgaccggc cggcattgcc ggaaatttat gcagctcacc gactttgacc cgttcgatcc cgcgattgcc gctgatcctt acccgcatta tcgagagctg ttggccggcg agcgggtgca gtacaacccc aagcgcgacg tctacatttt gagccgatac gccgacgtcc gcgaggctgc gcgtaaccac gacacgttgt ccagcgcccg tggggtcacc ttctcacggg ggtggctgcc gtttctgccg acgtccgatc cgccggcgca cacccggatg cgcaagcaac tggcaccagg tatggcgcgt ggcgcgttgg agacgtggcg cccgatggtc gaccagcttg cccgagaact ggtcggcggg ttactgaccc agacgcccgc ggacgtcgtc tccaccgtgg ccgcaccgat gccgatgcgc gctatcacca gtgtgctcgg cgtagatgga ccggacgagg ccgccttctg ccgtttgtcc aaccaggcgg tacggatcac cgacgttgcc ttgtcagcct ccgggttgat ttcgcttgtg cagggctttg ccggattccg gcgattgcgt gcactcttca ctcatcggcg tgacaacggg ctgcttaggg aatgcactgt tctcgggaag cttgccaccc atgccgagca aggtcgactc agcgatgacg aattgttctt cttcgcggtg ttgctgctgg ttgcgggcta tgagagcact gctcatatga ttagcacatt gtttctgacg ctggccgact atccagatca gctgacactc cttgcgcagc aaccagacct gatcccgtcg gcgatcgagg agcacctccg ctttatatcg ccaatccaaa acatctgccg cacaacgcgc gtcgactatt cggtcggtca agcggtcatc ccggcaggct cactggtgct gctggcatgg ggtgcagcca accgtgaccc gcgccagtac gaagacccgg atgtctttcg cgccgaccgc aacccggtcg ggcatctcgc gttcggctcc ggcatccacc tgtgtccggg gacccagctg gcgcgcatgg agggtcaggc gatcttgcgc gagatcgtcg ccaatatcga ccgaatagag gtggtcgagc cgccgacgtg gacgacaaac gccaaccttc gcggcttgac ccggttacgg gtcgccgtta ccccccgcgt cgcaccatga
Details for MytuD.18275.a.A1.GE34236
HARVESTED ON: 12/19/2011
SEQUENCED ON: 1/3/2012
EXPECTED MW: 55kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 23
PERCENT COVERAGE: 62
Validated AA Sequence
MAHHHHHHMX TLEAQTQGPG SMTATQSPPE PAPDRVRLAG CPLAGXPDVG LTAQDATTAL GVPTRRXXXX GXXXXXAXXX XXXTXKXXXX XXPXXXXXXL XXXXXXXXXX XXXXRXXXXX FXXXXXXXPX XXXNXXXXFX XPVGXXXXXX XPXXXXXXXX XXXXXRXXXX XXXXXAXXPG XXXXXGXGXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXX
Validated NT Sequence
ttttgtttaa ctttaagaag gagatatacc atggctcatc accatcacca tcatatgnnt accctggaag ctcagaccca gggtcctggt tcgatgaccg cgacacagtc ccctcccgag cccgcgccgg accgagtgcg gctggccggg tgcccgttag cgggcncncc cgacgttggt ctgaccgcnc aggacgcgac cacggctctg ggcgttccca cgcgccgnng nnnnnnnncn gggnggncnn ncncgnnngc cnnccannnn gnnnnngnga cggnnaaann nnnnnnannn nncngncccc nngnnnnnna aannnntttg nnnnnnnnnn nnnnnnggnn nnnnnnnnnn nnnnggnnnn nncggngnnn ttnnnnnnaa tttnntgnnn nnnnccnnnt ttncccgnnn nnncnnnnna accntnnncn nnnttttnnn anacctgttg gnngggnnnn ggntcnnnnn annccnnnnn nnnnnnnnnn nnttttnnnn cnnnnnnnnn nnnnccgnnn nnnnnnnnna ancnnnnnnn nnntngccnn gnncccnggg gnnnnccntn nnnnggggnn nggntnnnnn ttncnnnnnn nnngnnnnan ccnggntncn nnannnnnnn nnnnnnnnta ngnnnnnngn nnnnnnnnna nnnnnnnnnn nnnnnnnnnn nnnnnnnccn annngncnnn ngnnnnnnna nnnnaannnn nnnnnnnnnn ggnnnnnncn nnnncnnnnn nnnnnnnnnn nnntnnnnnn nnnnnnnnnn ncnnnnnnnn gnnnnnnnnn nntnnngnnn nnnnnnngnn nnnnnnnnnc nnnnnnnnnn nnnnnnnnnn nnngnnnnnn nnnnannnnn nnngnnnnnn nnnnnnncnn nnnnnnnann nnnnnnnnnn nnnngnnnnn nnnnnnnnng nnnnnnnnnn nnnnnnnnnn nannnntnnn nnnnnnnnnn na
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTATQSPPE PAPDRVRLAG CPLAGTPDVG LTAQDATTAL GVPTRRRASS GGIPVATSMW RDAQTVRTYG PAVAKALALR VAGKARSRLT GRHCRKFMQL TDFDPFDPAI AADPYPHYRE LLAGERVQYN PKRDVYILSR YADVREAARN HDTLSSARGV TFSRGWLPFL PTSDPPAHTR MRKQLAPGMA RGALETWRPM VDQLARELVG GLLTQTPADV VSTVAAPMPM RAITSVLGVD GPDEAAFCRL SNQAVRITDV ALSASGLISL VQGFAGFRRL RALFTHRRDN GLLRECTVLG KLATHAEQGR LSDDELFFFA VLLLVAGYES TAHMISTLFL TLADYPDQLT LLAQQPDLIP SAIEEHLRFI SPIQNICRTT RVDYSVGQAV IPAGSLVLLA WGAANRDPRQ YEDPDVFRAD RNPVGHLAFG SGIHLCPGTQ LARMEGQAIL REIVANIDRI EVVEPPTWTT NANLRGLTRL RVAVTPRVAP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgcgaca cagtcccctc ccgagcccgc gccggaccga gtgcggctgg ccgggtgccc gttagcgggc acgcccgacg ttggtctgac cgcgcaggac gcgaccacgg ctctgggcgt tcccacgcgc cggcgcgcgt catctggtgg catcccggta gccaccagca tgtggcgcga cgcgcaaact gtgcgaacct acgggcccgc ggtcgcgaaa gcacttgcgc tgagggtcgc cgggaaggct cggtccaggt tgaccggccg gcattgccgg aaatttatgc agctcaccga ctttgacccg ttcgatcccg cgattgccgc tgatccttac ccgcattatc gagagctgtt ggccggcgag cgggtgcagt acaaccccaa gcgcgacgtc tacattttga gccgatacgc cgacgtccgc gaggctgcgc gtaaccacga cacgttgtcc agcgcccgtg gggtcacctt ctcacggggg tggctgccgt ttctgccgac gtccgatccg ccggcgcaca cccggatgcg caagcaactg gcaccaggta tggcgcgtgg cgcgttggag acgtggcgcc cgatggtcga ccagcttgcc cgagaactgg tcggcgggtt actgacccag acgcccgcgg acgtcgtctc caccgtggcc gcaccgatgc cgatgcgcgc tatcaccagt gtgctcggcg tagatggacc ggacgaggcc gccttctgcc gtttgtccaa ccaggcggta cggatcaccg acgttgcctt gtcagcctcc gggttgattt cgcttgtgca gggctttgcc ggattccggc gattgcgtgc actcttcact catcggcgtg acaacgggct gcttagggaa tgcactgttc tcgggaagct tgccacccat gccgagcaag gtcgactcag cgatgacgaa ttgttcttct tcgcggtgtt gctgctggtt gcgggctatg agagcactgc tcatatgatt agcacattgt ttctgacgct ggccgactat ccagatcagc tgacactcct tgcgcagcaa ccagacctga tcccgtcggc gatcgaggag cacctccgct ttatatcgcc aatccaaaac atctgccgca caacgcgcgt cgactattcg gtcggtcaag cggtcatccc ggcaggctca ctggtgctgc tggcatgggg tgcagccaac cgtgacccgc gccagtacga agacccggat gtctttcgcg ccgaccgcaa cccggtcggg catctcgcgt tcggctccgg catccacctg tgtccgggga cccagctggc gcgcatggag ggtcaggcga tcttgcgcga gatcgtcgcc aatatcgacc gaatagaggt ggtcgagccg ccgacgtgga cgacaaacgc caaccttcgc ggcttgaccc ggttacgggt cgccgttacc ccccgcgtcg caccataaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa