MytuD.18256.b

Putative L,D-transpeptidase Rv0483/MT0501 (EC 2.-.-.-)

CENTER ID: MytuD.18256.b
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18256.b.B1.GE36546 full length 1 451
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.533
RefSeq: NP_214997.1
RefSeq: NP_334910.1
TubercuList: Rv0483
MTB Network Portal: Rv0483
UniProt: P9WKV3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVIRVLFRPV SLIPVNNSST PQSQGPISRR LALTALGFGV LAPNVLVACA GKVTKLAEKR PPPAPRLTFR PADSAADVVP IAPISVEVGD GWFQRVALTN SAGKVVAGAY SRDRTIYTIT EPLGYDTTYT WSGSAVGHDG KAVPVAGKFT TVAPVKTINA GFQLADGQTV GIAAPVIIQF DSPISDKAAV ERALTVTTDP PVEGGWAWLP DEAQGARVHW RPREYYPAGT TVDVDAKLYG LPFGDGAYGA QDMSLHFQIG RRQVVKAEVS SHRIQVVTDA GVIMDFPCSY GEADLARNVT RNGIHVVTEK YSDFYMSNPA AGYSHIHERW AVRISNNGEF IHANPMSAGA QGNSNVTNGC INLSTENAEQ YYRSAVYGDP VEVTGSSIQL SYADGDIWDW AVDWDTWVSM SALPPPAAKP AATQIPVTAP VTPSDAPTPS GTPTTTNGPG G
NT Sequence
gtggtcattc gtgtgctgtt tcgcccggta tctttgatac ccgtgaataa ctccagcacc ccccagagtc aggggccgat cagtcggcgt ctggcgttga cggcccttgg gtttggggtg ttggcaccga acgttctggt cgcgtgcgcc ggcaaagtga ccaagctggc cgagaagagg ccgccaccgg cgcctcgtct gactttccgg cctgccgact ctgccgccga cgtggtgccg atcgcgccga tcagcgtcga ggtcggtgac ggctggtttc agcgggtcgc gctgaccaat tcggcaggca aggtcgtcgc cggggcatac agccgggatc gcaccatcta cacgatcacc gagccgctgg gctacgacac gacctacacc tggagcggtt cggccgtcgg ccatgacggc aaggcggttc cggtggcggg caagttcacc accgtggcac ccgtcaagac gatcaacgcg ggattccagc tcgccgacgg ccagaccgtc gggatcgcgg cgccggtgat tattcagttc gattcaccga tcagcgacaa ggccgccgtc gagcgggcac taaccgtgac caccgacccg cctgtcgagg gcggctgggc ctggctgccc gacgaggcgc agggcgctcg cgtgcactgg cgtcctcggg agtactaccc ggcgggtacc accgtcgacg tcgacgccaa gctgtatggg ctgccgttcg gcgacggcgc gtacggcgcg caggatatgt cgttgcactt ccagatcggt cgtcgtcagg tggtcaaggc cgaagtctcg tcgcaccgca tccaagtcgt caccgatgcc ggcgtcatca tggacttccc gtgcagctac ggcgaggccg acttggcgcg caacgtcacc cgcaacggca tccacgtcgt caccgagaaa tactcggact tctacatgtc caacccggcc gccggttaca gccatatcca cgaacgttgg gcggtgcgga tttccaacaa cggcgagttc atccatgcca accctatgag cgccggtgcc cagggcaaca gcaatgtcac caacggctgt atcaacctgt cgacggagaa cgccgaacag tactaccgca gcgcggtcta cggtgacccg gttgaggtga ccggcagttc gatccagctg tcctacgccg acggtgacat ctgggactgg gcggtggact gggacacctg ggtgtcgatg tcggcgctac cgccaccggc ggccaaaccg gcggcgacgc aaatcccggt caccgccccg gtcacgccgt cggatgcccc caccccgtcc ggcacaccca cgactactaa cggaccgggt gggtag
Details for MytuD.18256.b.B1.GE36546
HARVESTED ON: 1/9/2013
SEQUENCED ON: 10/23/2013
EXPECTED MW: 49kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMV VIRVLFRPVS LIPVNNSSTP QSQGPISRRL ALTALGFGVL APNVLVACAG KVTKLAEKRP PPAPRLTFRP ADSAADVVPI APISVEVGDG WFQRVALTNS AGKVVAGAYS RDRTIYTITE PLGYDTTYTW SGSAVGHDGK AVPVAGKFTT VAPVKTINAG FQLADGQTVG IAAPVIIQFD SPISDKAAVE RALTVTTDPP VEGGWAWLPD EAQGARVHWR PREYYPAGTT VDVDAXLYGL PFGDGAYGAQ DMSLHFQIGR RQVVKAEVSS HRIQVVTDAG VIMDFPCSYG EADLARNVTR NGIHVVTEKY SDFYMSNPAA GYSHIHERWA VRISNNGEFI HANPMSAGAQ GNSNVTNGCI NLXTENAEQY YRSAVYGDPV EVTGSSIQLS YADGDIWDWA VDWDTWXXMS ALPPPAAKPA ATQIPVTAPV TPSDAPTPSG T
Validated NT Sequence
tttaagaagg aganatacca tggctcacca ccaccaccac catatggtgg tcattcgtgt gctgtttcgc ccggtatctt tgatacccgt gaataactcc agcacccccc agagtcaggg gccgatcagt cggcgtctgg cgttgacggc ccttgggttt ggggtgttgg caccgaacgt tctggtcgcg tgcgccggca aagtgaccaa gctggccgag aagaggccgc caccggcgcc tcgtctgact ttccggcctg ccgactctgc cgccgacgtg gtgccgatcg cgccgatcag cgtcgaggtc ggtgacggct ggtttcagcg ggtcgcgctg accaattcgg caggcaaggt cgtcgccggg gcatacagcc gggatcgcac catctacacg atcaccgagc cgctgggcta cgacacgacc tacacctgga gcggttcggc cgtcggccat gacggcaagg cggttccggt ggcgggcaag ttcaccaccg tggcacccgt caagacgatc aacgcgggat tccagctcgc cgacggccag accgtcggga tcgcggcgcc ggtgattatt cagttcgatt caccgatcag cgacaaggcc gccgtcgagc gggcactaac cgtgaccacc gacccgcctg tcgagggcgg ctgggcctgg ctgcccgacg aggcgcaggg cgctcgcgtg cactggcgtc ctcgggagta ctacccggcg ggtaccaccg tcgacgtcga cgccnagctg tatgggctgc cgttcggcga cggcgcgtac ggcgcgcagg atatgtcgtt gcacttccag atcggtcgtc gtcaggtggt caaggccgaa gtctcgtcgc accgcatcca agtcgtcacc gatgccggcg tcatcatgga cttcccgtgc agctacggcg aggccgactt ggcgcgcaac gtcacccgca acggcatcca cgtcgtcacc gagaaatact cggacttcta catgtccaac ccggccgccg gttacagcca tatccacgaa cgttgggcgg tgcggatttc caacaacggc gagttcatcc atgccaaccc tatgagcgcc ggtgcccagg gcaacagcaa tgtcaccaac ggctgtatca acctgtngac ggagaacgcc gaacagtact accgcagcgc ggtntacggt gacccggttg aggtgaccgg cagttcgatc cagctgtcnt acgccgacgg tgacatctgg gactgggcgg tggactggga cacctgggng tngatgtcgg cgctaccgcc accggcggcc aaaccggcgg cgacgcaaat cccggtcacc gccccggtca cgccgtcgga tgcccccacc ccgtccggca caccc
Expected Protein Sequence
MAHHHHHHMV VIRVLFRPVS LIPVNNSSTP QSQGPISRRL ALTALGFGVL APNVLVACAG KVTKLAEKRP PPAPRLTFRP ADSAADVVPI APISVEVGDG WFQRVALTNS AGKVVAGAYS RDRTIYTITE PLGYDTTYTW SGSAVGHDGK AVPVAGKFTT VAPVKTINAG FQLADGQTVG IAAPVIIQFD SPISDKAAVE RALTVTTDPP VEGGWAWLPD EAQGARVHWR PREYYPAGTT VDVDAKLYGL PFGDGAYGAQ DMSLHFQIGR RQVVKAEVSS HRIQVVTDAG VIMDFPCSYG EADLARNVTR NGIHVVTEKY SDFYMSNPAA GYSHIHERWA VRISNNGEFI HANPMSAGAQ GNSNVTNGCI NLSTENAEQY YRSAVYGDPV EVTGSSIQLS YADGDIWDWA VDWDTWVSMS ALPPPAAKPA ATQIPVTAPV TPSDAPTPSG TPTTTNGPGG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtgg tcattcgtgt gctgtttcgc ccggtatctt tgatacccgt gaataactcc agcacccccc agagtcaggg gccgatcagt cggcgtctgg cgttgacggc ccttgggttt ggggtgttgg caccgaacgt tctggtcgcg tgcgccggca aagtgaccaa gctggccgag aagaggccgc caccggcgcc tcgtctgact ttccggcctg ccgactctgc cgccgacgtg gtgccgatcg cgccgatcag cgtcgaggtc ggtgacggct ggtttcagcg ggtcgcgctg accaattcgg caggcaaggt cgtcgccggg gcatacagcc gggatcgcac catctacacg atcaccgagc cgctgggcta cgacacgacc tacacctgga gcggttcggc cgtcggccat gacggcaagg cggttccggt ggcgggcaag ttcaccaccg tggcacccgt caagacgatc aacgcgggat tccagctcgc cgacggccag accgtcggga tcgcggcgcc ggtgattatt cagttcgatt caccgatcag cgacaaggcc gccgtcgagc gggcactaac cgtgaccacc gacccgcctg tcgagggcgg ctgggcctgg ctgcccgacg aggcgcaggg cgctcgcgtg cactggcgtc ctcgggagta ctacccggcg ggtaccaccg tcgacgtcga cgccaagctg tatgggctgc cgttcggcga cggcgcgtac ggcgcgcagg atatgtcgtt gcacttccag atcggtcgtc gtcaggtggt caaggccgaa gtctcgtcgc accgcatcca agtcgtcacc gatgccggcg tcatcatgga cttcccgtgc agctacggcg aggccgactt ggcgcgcaac gtcacccgca acggcatcca cgtcgtcacc gagaaatact cggacttcta catgtccaac ccggccgccg gttacagcca tatccacgaa cgttgggcgg tgcggatttc caacaacggc gagttcatcc atgccaaccc tatgagcgcc ggtgcccagg gcaacagcaa tgtcaccaac ggctgtatca acctgtcgac ggagaacgcc gaacagtact accgcagcgc ggtctacggt gacccggttg aggtgaccgg cagttcgatc cagctgtcct acgccgacgg tgacatctgg gactgggcgg tggactggga cacctgggtg tcgatgtcgg cgctaccgcc accggcggcc aaaccggcgg cgacgcaaat cccggtcacc gccccggtca cgccgtcgga tgcccccacc ccgtccggca cacccacgac tactaacgga ccgggtgggt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat