MytuD.18256.a

POSSIBLE CONSERVED MEMBRANE PROTEIN

CENTER ID: MytuD.18256.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18256.a.A1.GE34210 full length 1 366
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18256.a.A1.PW34914 full length 1 366

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.217
RefSeq: NP_214706
TubercuList: Rv0192
MTB Network Portal: Rv0192
UniProt: O07436

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPHWAEERHR RESNYVALEA GLDEGESIRR SEHSRSGCGA DAGCWRCRGG PGRGSRRSRR SRGPGGTAGP VDPPAVDLLA PPPDPLALPP ALDPLAPPPP DPLAPPPPDP LAVPVAAGPV AGQDPTSFVG PPPFRPPTFN PVDGAMVGVA KPIVINFAVP IADRAMAESA IHISSIPPVP GKFYWMSPTQ VRWRPFEFWP ANTAVNIDAA GTKSSFRTGD SLVATADDAT HQMTITRNGV VQKTFPMSMG MVSGGHQTPN GTYYVLEKFA TVVMDSSTYG VPVNSAQGYK LTVSDAVRID NSGNFVHSAP WSVADQGKRN VTHGCINLSP ANAKWFYDNF GSGDPVVVKN SVGTYNKNDG AQDWQI
NT Sequence
atgccacact gggctgaaga acgtcaccgg agggaaagca attatgtcgc gctggaagca gggctggacg agggggagtc tattcgccgc tctgaacata gccgcagtgg ttgcggtgct gatgctgggt gctggcgttg ccgtggcgga cccggacgcg gctcccggcg atcccggagg tcccggggcc ccgggggcac agcgggaccc gtcgacccgc cggcagttga cctgttggcg ccgccacccg acccgttggc gctgccgccg gcacttgacc cgttggcgcc gccgccacct gacccgctcg cgccgccccc gcctgacccg ctggcagtgc cggtagcagc gggccccgtt gccgggcagg atccgacatc gtttgttggc ccgccgccgt tccggccgcc gacgttcaat ccggtcgacg gcgcgatggt cggtgtggcc aagccgatcg tcatcaactt cgcggtgccg atcgccgacc gggcgatggc cgaaagcgcc atccacattt cgtccatccc gcccgtgccg ggcaagttct actggatgag cccgactcag gtacgctggc gcccgtttga gttctggccc gccaacaccg cggtaaacat cgatgcggcc ggcaccaagt cgagcttccg gaccggtgat tcgctggtgg ccaccgccga cgacgccacg catcagatga caatcacccg caacggcgtc gtgcaaaaga ccttccccat gtcgatgggc atggtgtccg gcggccacca gaccccgaat ggcacctact acgtgcttga gaagttcgcc accgtggtca tggactcctc gacgtacggg gtcccggtca actcggccca aggctacaag ttgaccgtct ccgacgccgt ccggatcgac aacagcggca acttcgtgca cagcgcgccg tggtcggtgg cagatcaggg caagcgcaac gtcacccacg gctgcatcaa cctcagcccg gccaacgcga agtggttcta cgacaacttc ggcagcggtg acccggtcgt cgtgaagaac tctgtcggga cttacaacaa aaacgacggt gcccaggact ggcagatcta a
Details for MytuD.18256.a.A1.GE34210
HARVESTED ON: 12/19/2011
SEQUENCED ON: 12/10/2013
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPHWAEERH RRESNYVALE AGLDEGESIR RSEHSRSGCG ADAGCWRCRG GPGRGSRRSR RSRGPGGTAG PVDPPAVDLL APPPDPLALP PALDPLAPPP PDPLAPPPPD PLAVPVAAGP VAGQDPTSFV GPPPFRPPTF NPVDGAMVGV AKPIVINFAV PIADRAMAES AIHISSIPPV PGKFYWMSPT QVRWRPFEFW PANTAVNIDA AGTKSSFRTG DSLVATADDA THQMTITRNG VVQKTFPMSM GMVSGGHQTP NGTYYVLEKF ATVVMDSSTY GVPVNSAQGY KLTVSDAVRI DNSGNFVHSA PWSVADQGKR NVTHGCINLS PANAKWFYDN FGSGDPVVVK NSVGTYNKND GAQD
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgccac actgggctga agaacgtcac cggagggaaa gcaattatgt cgcgctggaa gcagggctgg acgaggggga gtctattcgc cgctctgaac atagccgcag tggttgcggt gctgatgctg ggtgctggcg ttgccgtggc ggacccggac gcggctcccg gcgatcccgg aggtcccggg gccccggggg cacagcggga cccgtcgacc cgccggcagt tgacctgttg gcgccgccac ccgacccgtt ggcgctgccg ccggcacttg acccgttggc gccgccgcca cctgacccgc tcgcgccgcc cccgcctgac ccgctggcag tgccggtagc agcgggcccc gttgccgggc aggatccgac atcgtttgtt ggcccgccgc cgttccggcc gccgacgttc aatccggtcg acggcgcgat ggtcggtgtg gccaagccga tcgtcatcaa cttcgcggtg ccgatcgccg accgggcgat ggccgaaagc gccatccaca tttcgtccat cccgcccgtg ccgggcaagt tctactggat gagcccgact caggtacgct ggcgcccgtt tgagttctgg cccgccaaca ccgcggtaaa catcgatgcg gccggcacca agtcgagctt ccggaccggt gattcgctgg tggccaccgc cgacgacgcc acgcatcaga tgacaatcac ccgcaacggc gtcgtgcaaa agaccttccc catgtcgatg ggcatggtgt ccggcggcca ccagaccccg aatggcacct actacgtgct tgagaagttc gccaccgtgg tcatggactc ctcgacgtac ggggtcccgg tcaactcggc ccaaggctac aagttgaccg tctccgacgc cgtccggatc gacaacagcg gcaacttcgt gcacagcgcg ccgtggtcgg tggcagatca gggcaagcgc aacgtcaccc acggctgcat caacctcagc ccggccaacg cgaagtggtt ctacgacaac ttcggcagcg gtgacccggt cgtcgtgaag aactctgtcg ggacttacaa caaaaacgac ggtgcccagg actgg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPHWAEERH RRESNYVALE AGLDEGESIR RSEHSRSGCG ADAGCWRCRG GPGRGSRRSR RSRGPGGTAG PVDPPAVDLL APPPDPLALP PALDPLAPPP PDPLAPPPPD PLAVPVAAGP VAGQDPTSFV GPPPFRPPTF NPVDGAMVGV AKPIVINFAV PIADRAMAES AIHISSIPPV PGKFYWMSPT QVRWRPFEFW PANTAVNIDA AGTKSSFRTG DSLVATADDA THQMTITRNG VVQKTFPMSM GMVSGGHQTP NGTYYVLEKF ATVVMDSSTY GVPVNSAQGY KLTVSDAVRI DNSGNFVHSA PWSVADQGKR NVTHGCINLS PANAKWFYDN FGSGDPVVVK NSVGTYNKND GAQDWQI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccacactgg gctgaagaac gtcaccggag ggaaagcaat tatgtcgcgc tggaagcagg gctggacgag ggggagtcta ttcgccgctc tgaacatagc cgcagtggtt gcggtgctga tgctgggtgc tggcgttgcc gtggcggacc cggacgcggc tcccggcgat cccggaggtc ccggggcccc gggggcacag cgggacccgt cgacccgccg gcagttgacc tgttggcgcc gccacccgac ccgttggcgc tgccgccggc acttgacccg ttggcgccgc cgccacctga cccgctcgcg ccgcccccgc ctgacccgct ggcagtgccg gtagcagcgg gccccgttgc cgggcaggat ccgacatcgt ttgttggccc gccgccgttc cggccgccga cgttcaatcc ggtcgacggc gcgatggtcg gtgtggccaa gccgatcgtc atcaacttcg cggtgccgat cgccgaccgg gcgatggccg aaagcgccat ccacatttcg tccatcccgc ccgtgccggg caagttctac tggatgagcc cgactcaggt acgctggcgc ccgtttgagt tctggcccgc caacaccgcg gtaaacatcg atgcggccgg caccaagtcg agcttccgga ccggtgattc gctggtggcc accgccgacg acgccacgca tcagatgaca atcacccgca acggcgtcgt gcaaaagacc ttccccatgt cgatgggcat ggtgtccggc ggccaccaga ccccgaatgg cacctactac gtgcttgaga agttcgccac cgtggtcatg gactcctcga cgtacggggt cccggtcaac tcggcccaag gctacaagtt gaccgtctcc gacgccgtcc ggatcgacaa cagcggcaac ttcgtgcaca gcgcgccgtg gtcggtggca gatcagggca agcgcaacgt cacccacggc tgcatcaacc tcagcccggc caacgcgaag tggttctacg acaacttcgg cagcggtgac ccggtcgtcg tgaagaactc tgtcgggact tacaacaaaa acgacggtgc ccaggactgg cagatctaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for MytuD.18256.a.A1.PW34914
PURIFICATION DATe: 2/3/2012
CONCENTRATION: 7.59mg/ml
OBSERVED MW: 44kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPHWAEERH RRESNYVALE AGLDEGESIR RSEHSRSGCG ADAGCWRCRG GPGRGSRRSR RSRGPGGTAG PVDPPAVDLL APPPDPLALP PALDPLAPPP PDPLAPPPPD PLAVPVAAGP VAGQDPTSFV GPPPFRPPTF NPVDGAMVGV AKPIVINFAV PIADRAMAES AIHISSIPPV PGKFYWMSPT QVRWRPFEFW PANTAVNIDA AGTKSSFRTG DSLVATADDA THQMTITRNG VVQKTFPMSM GMVSGGHQTP NGTYYVLEKF ATVVMDSSTY GVPVNSAQGY KLTVSDAVRI DNSGNFVHSA PWSVADQGKR NVTHGCINLS PANAKWFYDN FGSGDPVVVK NSVGTYNKND GAQD
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgccac actgggctga agaacgtcac cggagggaaa gcaattatgt cgcgctggaa gcagggctgg acgaggggga gtctattcgc cgctctgaac atagccgcag tggttgcggt gctgatgctg ggtgctggcg ttgccgtggc ggacccggac gcggctcccg gcgatcccgg aggtcccggg gccccggggg cacagcggga cccgtcgacc cgccggcagt tgacctgttg gcgccgccac ccgacccgtt ggcgctgccg ccggcacttg acccgttggc gccgccgcca cctgacccgc tcgcgccgcc cccgcctgac ccgctggcag tgccggtagc agcgggcccc gttgccgggc aggatccgac atcgtttgtt ggcccgccgc cgttccggcc gccgacgttc aatccggtcg acggcgcgat ggtcggtgtg gccaagccga tcgtcatcaa cttcgcggtg ccgatcgccg accgggcgat ggccgaaagc gccatccaca tttcgtccat cccgcccgtg ccgggcaagt tctactggat gagcccgact caggtacgct ggcgcccgtt tgagttctgg cccgccaaca ccgcggtaaa catcgatgcg gccggcacca agtcgagctt ccggaccggt gattcgctgg tggccaccgc cgacgacgcc acgcatcaga tgacaatcac ccgcaacggc gtcgtgcaaa agaccttccc catgtcgatg ggcatggtgt ccggcggcca ccagaccccg aatggcacct actacgtgct tgagaagttc gccaccgtgg tcatggactc ctcgacgtac ggggtcccgg tcaactcggc ccaaggctac aagttgaccg tctccgacgc cgtccggatc gacaacagcg gcaacttcgt gcacagcgcg ccgtggtcgg tggcagatca gggcaagcgc aacgtcaccc acggctgcat caacctcagc ccggccaacg cgaagtggtt ctacgacaac ttcggcagcg gtgacccggt cgtcgtgaag aactctgtcg ggacttacaa caaaaacgac ggtgcccagg actgg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPHWAEERH RRESNYVALE AGLDEGESIR RSEHSRSGCG ADAGCWRCRG GPGRGSRRSR RSRGPGGTAG PVDPPAVDLL APPPDPLALP PALDPLAPPP PDPLAPPPPD PLAVPVAAGP VAGQDPTSFV GPPPFRPPTF NPVDGAMVGV AKPIVINFAV PIADRAMAES AIHISSIPPV PGKFYWMSPT QVRWRPFEFW PANTAVNIDA AGTKSSFRTG DSLVATADDA THQMTITRNG VVQKTFPMSM GMVSGGHQTP NGTYYVLEKF ATVVMDSSTY GVPVNSAQGY KLTVSDAVRI DNSGNFVHSA PWSVADQGKR NVTHGCINLS PANAKWFYDN FGSGDPVVVK NSVGTYNKND GAQDWQI
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCACACTGG GCTGAAGAAC GTCACCGGAG GGAAAGCAAT TATGTCGCGC TGGAAGCAGG GCTGGACGAG GGGGAGTCTA TTCGCCGCTC TGAACATAGC CGCAGTGGTT GCGGTGCTGA TGCTGGGTGC TGGCGTTGCC GTGGCGGACC CGGACGCGGC TCCCGGCGAT CCCGGAGGTC CCGGGGCCCC GGGGGCACAG CGGGACCCGT CGACCCGCCG GCAGTTGACC TGTTGGCGCC GCCACCCGAC CCGTTGGCGC TGCCGCCGGC ACTTGACCCG TTGGCGCCGC CGCCACCTGA CCCGCTCGCG CCGCCCCCGC CTGACCCGCT GGCAGTGCCG GTAGCAGCGG GCCCCGTTGC CGGGCAGGAT CCGACATCGT TTGTTGGCCC GCCGCCGTTC CGGCCGCCGA CGTTCAATCC GGTCGACGGC GCGATGGTCG GTGTGGCCAA GCCGATCGTC ATCAACTTCG CGGTGCCGAT CGCCGACCGG GCGATGGCCG AAAGCGCCAT CCACATTTCG TCCATCCCGC CCGTGCCGGG CAAGTTCTAC TGGATGAGCC CGACTCAGGT ACGCTGGCGC CCGTTTGAGT TCTGGCCCGC CAACACCGCG GTAAACATCG ATGCGGCCGG CACCAAGTCG AGCTTCCGGA CCGGTGATTC GCTGGTGGCC ACCGCCGACG ACGCCACGCA TCAGATGACA ATCACCCGCA ACGGCGTCGT GCAAAAGACC TTCCCCATGT CGATGGGCAT GGTGTCCGGC GGCCACCAGA CCCCGAATGG CACCTACTAC GTGCTTGAGA AGTTCGCCAC CGTGGTCATG GACTCCTCGA CGTACGGGGT CCCGGTCAAC TCGGCCCAAG GCTACAAGTT GACCGTCTCC GACGCCGTCC GGATCGACAA CAGCGGCAAC TTCGTGCACA GCGCGCCGTG GTCGGTGGCA GATCAGGGCA AGCGCAACGT CACCCACGGC TGCATCAACC TCAGCCCGGC CAACGCGAAG TGGTTCTACG ACAACTTCGG CAGCGGTGAC CCGGTCGTCG TGAAGAACTC TGTCGGGACT TACAACAAAA ACGACGGTGC CCAGGACTGG CAGATCTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA