SSGCID: Target Page

MytuD.18223.a

PPE FAMILY PROTEIN

CENTER ID:	MytuD.18223.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.18223.a.A1.GE34216	full length		1	536

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|83332.12.peg.312
RefSeq:	YP_177709
TubercuList:	Rv0280
MTB Network Portal:	Rv0280
UniProt:	P9WI45

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTLWMASPPE VHSALLSSGP GPGSVLSAAG VWSSLSAEYA AVADELIGLL GAVQTGAWQG PSAAAYVAAH APYLAWLMRA SETSAEAAAR HETVAAAYTT AVAAMPTLVE LAANHTLHGV LVATNFFGIN TIPIALNEAD YARMWTQAAS TMATYQAVAE AAVASAPQTT PAPPILAAEA ADDDHDHDHD HGGEPTPLDY LVAEILRIIS GGRLIWDPAE GTMNGIPFED YTDAAQPIWW VVRAIEFSKD FETFVQELFV NPVEAFQFYF ELLLFDYPTH IVQIVEALSQ SPQLLAVALG SVISNLGAVT GFAGLSGLAG MQPAAIPALA PVAAAPSTLP AVAMAPTMAA PGAAVASAAA PASAPAASTV ASATPAPPPA PGAAGFGYPY AIAPPGIGFG SGMSASASAQ RKAPQPDSAA AAAAAAAVRD QARARRRRRV TRRGYGDEFM DMNIDVDPDW GPPPGEDPVT STVASDRGAG HLGFAGTARR EAVADAAGMT TLAGDDFGDG PTTPMVPGSW DPDRDAPGSA EPGDRG

NT Sequence

atgacgctgt ggatggcctc gccgcccgag gtgcattcgg cgttgctcag cagcgggccg gggccgggct cggtgttgtc ggcggccggg gtgtggtcgt cgctgagcgc cgaatacgcc gcggtcgccg acgagctcat agggctgctg ggcgccgtgc agaccggcgc ttggcagggg cccagcgccg cggcttatgt ggccgcccac gcgccgtacc tcgcgtggtt aatgcgggcc agcgaaacca gcgcggaagc ggccgcccgg cacgagaccg tggccgcggc ctacacgacc gcggtggcgg ccatgccgac gttggtcgag ctggccgcca accacacgct tcacggggtc ttggtggcga cgaacttctt cggcatcaac accatcccga tcgcgctcaa cgaggccgac tacgcgcgga tgtggacgca ggccgccagc acgatggcga cctatcaagc ggtcgccgag gccgcggtgg cgtcggcacc gcagaccacc ccggcgccgc cgatcttggc agccgaagcg gccgacgatg accacgatca tgaccacgat cacgggggcg aaccgacccc gctggactat ctggtcgcgg agatattgcg catcatcagc ggtgggcgcc tgatctggga tcccgccgag ggcaccatga acggaatccc gttcgaagat tatacggacg cagcccaacc aatctggtgg gttgttcgtg ccatcgaatt cagtaaggac tttgaaacgt ttgttcagga actgtttgtc aatccggtgg aggcatttca gttctacttt gagcttctat tgttcgacta cccgacccac attgtgcaga ttgttgaggc gttgagccag tccccgcagt tgctggcggt cgcactcggt tccgtcatct ccaacttggg tgcggtgacc gggttcgccg ggctatccgg cttggccggc atgcagccgg cggctatccc ggcgctagca cccgtcgcgg cggccccgtc gacattgccg gcggtcgcga tggccccgac catggccgcg ccgggcgcgg cggttgcgtc ggcagccgcg ccggcgtccg cgccggcggc cagcacggtg gccagcgcca cgccggcacc gccgccggca cccggcgccg ccgggttcgg ctatccctac gccatcgctc cgcccggcat cgggttcggc tcggggatga gcgccagcgc cagcgctcaa cgcaaggcac cacagcccga tagtgcggcg gcggcggcgg ccgcggcggc cgtacgtgac caagcgcggg cgcggcggcg gcgccgtgtc acgcggcgcg gatacggcga cgagtttatg gatatgaaca tcgacgtcga tccggactgg ggccctccgc ccggcgaaga cccagtcaca tccacggtgg cctcggatcg gggtgccgga catctgggct ttgccgggac ggcccgcagg gaggcggttg ccgacgcggc cgggatgacc acgctggctg gcgatgattt cggcgacggg ccaacgacgc caatggtgcc gggttcgtgg gatccggacc gggatgcgcc tggctcggcg gagcctggag atcggggctg a

Details for MytuD.18223.a.A1.GE34216

HARVESTED ON:	12/19/2011
SEQUENCED ON:	1/3/2012
EXPECTED MW:	56kDa
OBSERVED MW:	56kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	92
PERCENT COVERAGE:	10

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMTLWMASPP EVHSALLXSG PGPGSVLSXX XVWSSL

Validated NT Sequence

ttaagaagga gnnntaccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgacgctg tggatggcct cgccgcccga ggtgcattcg gcgttgctca ncagcgggcc ggggccgggc tcggtgttgt cngnngnnng ngtgtggtcg tcgctga

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTLWMASPP EVHSALLSSG PGPGSVLSAA GVWSSLSAEY AAVADELIGL LGAVQTGAWQ GPSAAAYVAA HAPYLAWLMR ASETSAEAAA RHETVAAAYT TAVAAMPTLV ELAANHTLHG VLVATNFFGI NTIPIALNEA DYARMWTQAA STMATYQAVA EAAVASAPQT TPAPPILAAE AADDDHDHDH DHGGEPTPLD YLVAEILRII SGGRLIWDPA EGTMNGIPFE DYTDAAQPIW WVVRAIEFSK DFETFVQELF VNPVEAFQFY FELLLFDYPT HIVQIVEALS QSPQLLAVAL GSVISNLGAV TGFAGLSGLA GMQPAAIPAL APVAAAPSTL PAVAMAPTMA APGAAVASAA APASAPAAST VASATPAPPP APGAAGFGYP YAIAPPGIGF GSGMSASASA QRKAPQPDSA AAAAAAAAVR DQARARRRRR VTRRGYGDEF MDMNIDVDPD WGPPPGEDPV TSTVASDRGA GHLGFAGTAR REAVADAAGM TTLAGDDFGD GPTTPMVPGS WDPDRDAPGS AEPGDRG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgctgtgg atggcctcgc cgcccgaggt gcattcggcg ttgctcagca gcgggccggg gccgggctcg gtgttgtcgg cggccggggt gtggtcgtcg ctgagcgccg aatacgccgc ggtcgccgac gagctcatag ggctgctggg cgccgtgcag accggcgctt ggcaggggcc cagcgccgcg gcttatgtgg ccgcccacgc gccgtacctc gcgtggttaa tgcgggccag cgaaaccagc gcggaagcgg ccgcccggca cgagaccgtg gccgcggcct acacgaccgc ggtggcggcc atgccgacgt tggtcgagct ggccgccaac cacacgcttc acggggtctt ggtggcgacg aacttcttcg gcatcaacac catcccgatc gcgctcaacg aggccgacta cgcgcggatg tggacgcagg ccgccagcac gatggcgacc tatcaagcgg tcgccgaggc cgcggtggcg tcggcaccgc agaccacccc ggcgccgccg atcttggcag ccgaagcggc cgacgatgac cacgatcatg accacgatca cgggggcgaa ccgaccccgc tggactatct ggtcgcggag atattgcgca tcatcagcgg tgggcgcctg atctgggatc ccgccgaggg caccatgaac ggaatcccgt tcgaagatta tacggacgca gcccaaccaa tctggtgggt tgttcgtgcc atcgaattca gtaaggactt tgaaacgttt gttcaggaac tgtttgtcaa tccggtggag gcatttcagt tctactttga gcttctattg ttcgactacc cgacccacat tgtgcagatt gttgaggcgt tgagccagtc cccgcagttg ctggcggtcg cactcggttc cgtcatctcc aacttgggtg cggtgaccgg gttcgccggg ctatccggct tggccggcat gcagccggcg gctatcccgg cgctagcacc cgtcgcggcg gccccgtcga cattgccggc ggtcgcgatg gccccgacca tggccgcgcc gggcgcggcg gttgcgtcgg cagccgcgcc ggcgtccgcg ccggcggcca gcacggtggc cagcgccacg ccggcaccgc cgccggcacc cggcgccgcc gggttcggct atccctacgc catcgctccg cccggcatcg ggttcggctc ggggatgagc gccagcgcca gcgctcaacg caaggcacca cagcccgata gtgcggcggc ggcggcggcc gcggcggccg tacgtgacca agcgcgggcg cggcggcggc gccgtgtcac gcggcgcgga tacggcgacg agtttatgga tatgaacatc gacgtcgatc cggactgggg ccctccgccc ggcgaagacc cagtcacatc cacggtggcc tcggatcggg gtgccggaca tctgggcttt gccgggacgg cccgcaggga ggcggttgcc gacgcggccg ggatgaccac gctggctggc gatgatttcg gcgacgggcc aacgacgcca atggtgccgg gttcgtggga tccggaccgg gatgcgcctg gctcggcgga gcctggagat cggggctaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa