MytuD.18214.i

PPE FAMILY PROTEIN

CENTER ID: MytuD.18214.i
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18214.i.A1.GE34217 full length 1 479
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.3953
RefSeq: YP_177987
TubercuList: Rv3539
MTB Network Portal: Rv3539
UniProt: P9WHX5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MADFLTLSPE VNSARMYAGG GPGSLSAAAA AWDELAAELW LAAASFESVC SGLADRWWQG PSSRMMAAQA ARHTGWLAAA ATQAEGAASQ AQTMALAYEA AFAATVHPAL VAANRALVAW LAGSNVFGQN TPAIAAAEAI YEQMWAQDVV AMLNYHAVAS AVGARLRPWQ QLLHELPRRL GGEHSDSTNT ELANPSSTTT RITVPGASPV HAATLLPFIG RLLAARYAEL NTAIGTNWFP GTTPEVVSYP ATIGVLSGSL GAVDANQSIA IGQQMLHNEI LAATASGQPV TVAGLSMGSM VIDRELAYLA IDPNAPPSSA LTFVELAGPE RGLAQTYLPV GTTIPIAGYT VGNAPESQYN TSVVYSQYDI WADPPDRPWN LLAGANALMG AAYFHDLTAY AAPQQGIEIA AVTSSLGGTT TTYMIPSPTL PLLLPLKQIG VPDWIVGGLN NVLKPLVDAG YSQYAPTAGP YFSHGNLVW
NT Sequence
atggcggatt tcttgacgtt gtcaccagag gtgaattcgg cccggatgta cgcgggtggg gggcccgggt cgctatcggc ggccgcggcg gcctgggatg agttggccgc cgaactgtgg ttggcggcgg cctcgttcga gtcggtgtgc tccggcctgg cggaccgttg gtggcaaggg ccgtcgtctc ggatgatggc ggcgcaggcc gcccgccata cggggtggct ggccgcggcg gccacccagg cagagggagc agccagccag gctcagacga tggcgctggc ctatgaagcg gcgttcgccg caaccgtaca cccggcgctg gtcgcggcga accgcgccct cgtggcctgg ttggcggggt cgaatgtgtt cgggcagaac accccggcga ttgcggccgc cgaggccatc tacgagcaga tgtgggctca ggatgttgtc gcgatgttga actaccatgc ggtggcctcg gcggtcgggg cgcggttgcg gccgtggcag cagttgctgc atgagctgcc caggcggttg ggcggcgaac actccgacag cacaaacacg gaactcgcta acccgagttc aacgacgaca cgcattaccg tccccggcgc atctccggtg catgcagcga cgttactgcc gttcatcgga aggctactgg cggcgcgtta tgccgagctg aacaccgcga tcggcacgaa ctggtttccg ggcaccacgc cagaagtggt gagctatccg gccaccatcg gggtccttag cggctctctt ggcgccgtcg atgccaacca gtccatcgct atcggtcagc agatgttgca caacgagatc ctggccgcca cggcctccgg tcagccggtg acggtggccg gactgtcgat gggcagcatg gtcatcgacc gcgaacttgc ctatctggcc atcgacccca acgcgccacc ctcgagcgcg ctcacattcg tcgagctcgc cggcccggaa cgcggtcttg cccagaccta cctgcccgtt ggcaccacca ttccaatcgc ggggtacacc gtggggaatg cgcccgagag ccagtacaac accagcgtgg tttatagcca gtacgatatc tgggccgatc cgcccgaccg tccgtggaac ctgttggccg gcgccaacgc actgatgggc gcggcttact ttcacgatct gaccgcctac gccgcaccac aacaggggat agagatcgcc gctgtcacga gttcactggg cggaaccacg acaacgtaca tgattccgtc gcccacgctg ccgttgctgt tgccactgaa gcagatcggt gtcccagact ggatcgtcgg cgggctgaac aacgtgctga agccgctcgt cgacgcgggc tactcacagt acgcccccac cgccggccct tatttcagcc acggcaacct ggtgtggtag
Details for MytuD.18214.i.A1.GE34217
HARVESTED ON: 12/19/2011
SEQUENCED ON: 12/28/2011
EXPECTED MW: 52kDa
OBSERVED MW: 52kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 82
PERCENT COVERAGE: 73
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMADFLTLSP EVNSARMYAG GGPGSLSAAA AAWDELAAEL WLAAASFESV CSGLADRWWQ GPSSRMMAAQ AARHTGWLAA AATQAEGAAS QAQTMALAYE AAFAATVHPA LVAANRALVA WLAGSNVFGQ NTPAIAAAEA IYEQMWAQDV VAMLNYHAVA SAVGARLRPW QQLLHELPRR LGGEHSDSTX TELANPSSTT TRITVPGASP VHAATLLPFI GRLLAARYAE LNTAIGTNWF PGTTPEVVSY PATIGVLSGS LGAVDAXQSI AIGQQMLHNE SWXXXXXSAX XXXXXRWXAX XRXXXXXXXT XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXX
Validated NT Sequence
attttnttta ctttangaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcgg atttcttgac gttgtcacca gaggtgaatt cggcccggat gtacgcgggt ggggggcccg ggtcgctatc ggcggccgcg gcggcctggg atgagttggc cgccgaactg tggttggcgg cggcctcgtt cgagtcggtg tgctccggcc tggcggaccg ttggtggcaa gggccgtcgt ctcggatgat ggcggcgcag gccgcccgcc atacggggtg gctggccgcg gcggccaccc aggcagaggg agcagccagc caggctcaga cgatggcgct ggcctatgaa gcggcgttcg ccgcaaccgt acacccggcg ctggtcgcgg cgaaccgcgc cctcgtggcc tggttggcgg ggtcgaatgt gttcgggcag aacaccccgg cgattgcggc cgccgaggcc atctacgagc agatgtgggc tcaggatgtt gtcgcgatgt tgaactacca tgcggtggcc tcggcggtcg gggcgcggtt gcggccgtgg cagcagttgc tgcatgagct gcccaggcgg ttgggcggcg aacactccga cagcacanac acggaactcg ctaacccgag ttcaacgacg acacgcatta ccgtccccgg cgcatctccg gtgcatgcag cgacgttact gccgttcatc ggaaggctac tggcggcgcg ttatgccgag ctgaacaccg cgatcggcac gaactggttt ccgggcacca cgccagaagt ggtgagctat ccggccacca tcggggtcct tagcggctct cttggngccg tcgatgccna ccagtccatc gctatcggtc agcagatgtt gcacaacgag tcntggnngc nncnnnnncn gtcagcngnn gannnngnnn actgncgatg ggnagcatgn natcganngc nantnnnntn tnnntngacc nnnnnnnnnt nnnntnntcn anntnnnnnn nnnnnnnntn nnnnnnnnnc nnnnnnnnnn nnntnntnnn nnnnnnnnnn nngnannnng nnnnnnnnnn nnnnnnnnnn nnnnnng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMADFLTLSP EVNSARMYAG GGPGSLSAAA AAWDELAAEL WLAAASFESV CSGLADRWWQ GPSSRMMAAQ AARHTGWLAA AATQAEGAAS QAQTMALAYE AAFAATVHPA LVAANRALVA WLAGSNVFGQ NTPAIAAAEA IYEQMWAQDV VAMLNYHAVA SAVGARLRPW QQLLHELPRR LGGEHSDSTN TELANPSSTT TRITVPGASP VHAATLLPFI GRLLAARYAE LNTAIGTNWF PGTTPEVVSY PATIGVLSGS LGAVDANQSI AIGQQMLHNE ILAATASGQP VTVAGLSMGS MVIDRELAYL AIDPNAPPSS ALTFVELAGP ERGLAQTYLP VGTTIPIAGY TVGNAPESQY NTSVVYSQYD IWADPPDRPW NLLAGANALM GAAYFHDLTA YAAPQQGIEI AAVTSSLGGT TTTYMIPSPT LPLLLPLKQI GVPDWIVGGL NNVLKPLVDA GYSQYAPTAG PYFSHGNLVW
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggatttc ttgacgttgt caccagaggt gaattcggcc cggatgtacg cgggtggggg gcccgggtcg ctatcggcgg ccgcggcggc ctgggatgag ttggccgccg aactgtggtt ggcggcggcc tcgttcgagt cggtgtgctc cggcctggcg gaccgttggt ggcaagggcc gtcgtctcgg atgatggcgg cgcaggccgc ccgccatacg gggtggctgg ccgcggcggc cacccaggca gagggagcag ccagccaggc tcagacgatg gcgctggcct atgaagcggc gttcgccgca accgtacacc cggcgctggt cgcggcgaac cgcgccctcg tggcctggtt ggcggggtcg aatgtgttcg ggcagaacac cccggcgatt gcggccgccg aggccatcta cgagcagatg tgggctcagg atgttgtcgc gatgttgaac taccatgcgg tggcctcggc ggtcggggcg cggttgcggc cgtggcagca gttgctgcat gagctgccca ggcggttggg cggcgaacac tccgacagca caaacacgga actcgctaac ccgagttcaa cgacgacacg cattaccgtc cccggcgcat ctccggtgca tgcagcgacg ttactgccgt tcatcggaag gctactggcg gcgcgttatg ccgagctgaa caccgcgatc ggcacgaact ggtttccggg caccacgcca gaagtggtga gctatccggc caccatcggg gtccttagcg gctctcttgg cgccgtcgat gccaaccagt ccatcgctat cggtcagcag atgttgcaca acgagatcct ggccgccacg gcctccggtc agccggtgac ggtggccgga ctgtcgatgg gcagcatggt catcgaccgc gaacttgcct atctggccat cgaccccaac gcgccaccct cgagcgcgct cacattcgtc gagctcgccg gcccggaacg cggtcttgcc cagacctacc tgcccgttgg caccaccatt ccaatcgcgg ggtacaccgt ggggaatgcg cccgagagcc agtacaacac cagcgtggtt tatagccagt acgatatctg ggccgatccg cccgaccgtc cgtggaacct gttggccggc gccaacgcac tgatgggcgc ggcttacttt cacgatctga ccgcctacgc cgcaccacaa caggggatag agatcgccgc tgtcacgagt tcactgggcg gaaccacgac aacgtacatg attccgtcgc ccacgctgcc gttgctgttg ccactgaagc agatcggtgt cccagactgg atcgtcggcg ggctgaacaa cgtgctgaag ccgctcgtcg acgcgggcta ctcacagtac gcccccaccg ccggccctta tttcagccac ggcaacctgg tgtggtaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa