SSGCID: Target Page

MytuD.18214.h

PPE FAMILY PROTEIN

CENTER ID:	MytuD.18214.h
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.18214.h.B1.GE39934	full length		1	380

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|83332.12.peg.3501
RefSeq:	YP_177935
TubercuList:	Rv3136
MTB Network Portal:	Rv3136
UniProt:	P9WHY3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDFALLPPEV NSARMYTGPG AGSLLAAAGG WDSLAAELAT TAEAYGSVLS GLAALHWRGP AAESMAVTAA PYIGWLYTTA EKTQQTAIQA RAAALAFEQA YAMTLPPPVV AANRIQLLAL IATNFFGQNT AAIAATEAQY AEMWAQDAAA MYGYATASAA AALLTPFSPP RQTTNPAGLT AQAAAVSQAT DPLSLLIETV TQALQALTIP SFIPEDFTFL DAIFAGYATV GVTQDVESFV AGTIGAESNL GLLNVGDENP AEVTPGDFGI GELVSATSPG GGVSASGAGG AASVGNTVLA SVGRANSIGQ LSVPPSWAAP STRPVSALSP AGLTTLPGTD VAEHGMPGVP GVPVAAGRAS GVLPRYGVRL TVMAHPPAAG

NT Sequence

atggatttcg cactgttacc accggaagtc aactccgccc ggatgtacac cggccctggg gcaggatcgc tgttggctgc cgcgggcggc tgggattcgc tggccgccga gttggccacc acagccgagg catatggatc ggtgctgtcc ggactggccg ccttgcattg gcgtggaccg gcagcggaat cgatggcggt gacggccgct ccctatatcg gttggctgta cacgaccgcc gaaaagacac agcaaacagc gatccaagcc agggcggcag cgctggcctt cgagcaagca tacgcaatga ccctgccgcc accggtggta gcggccaacc ggatacagct gctagcactg atcgcgacga acttcttcgg ccagaacact gcggcgatcg cggccaccga ggcacagtac gccgagatgt gggcccagga cgccgccgcg atgtacggtt acgccaccgc ctcagcggct gcggccctgc tgacaccgtt ctccccgccg cggcagacca ccaacccggc cggcctgacc gctcaggccg ccgcggtcag ccaggccacc gacccactgt cgctgctgat tgagacggtg acccaagcgc tgcaagcgct gacgattccg agcttcatcc ctgaggactt caccttcctt gacgccatat tcgctggata tgccacggta ggtgtgacgc aggatgtcga gtcctttgtt gccgggacca tcggggccga gagcaaccta ggccttttga acgtcggcga cgagaatccc gcggaggtga caccgggcga ctttgggatc ggcgagttgg tttccgcgac cagtcccggc ggtggggtgt ctgcgtcggg tgccggcggt gcggcgagcg tcggcaacac ggtgctcgcg agtgtcggcc gggcaaactc gattgggcaa ctatcggtcc caccgagctg ggccgcgccc tcgacgcgcc ctgtctcggc attgtcgccc gccggcctga ccacactccc ggggaccgac gtggccgagc acgggatgcc aggtgtaccg ggggtgccag tggcagcagg gcgagcctcc ggcgtcctac ctcgatacgg ggttcggctc acggtgatgg cccacccacc cgcggcaggg taa

Details for MytuD.18214.h.B1.GE39934

HARVESTED ON:	2/26/2016
SEQUENCED ON:	3/1/2016
EXPECTED MW:	39kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMD FALLPPEVNS ARMYTGPGAG SLLAAAGGWD SLAAELATTA EAYGSVLSGL AALHWRGPAA ESMAVTAAPY IGWLYTTAEK TQQTAIQARA AALAFEQAYA MTLPPPVVAA NRIQLLALIA TNFFGQNTAA IAATEAQYAE MWAQDAAAMY GYATASAAAA LLTPFSPPRQ TTNPAGLTAQ AAAVSQATDP LSLLIETVTQ ALQALTIPSF IPEDFTFLDA IFAGYATVGV TQDVESFVAG TIGAESNLGL LNVGDENPAE VTPGDFGIGE LVSATSPGGG VSASGAGGAA SVGNTVLASV GRANSIGQLS VPPSWAAPST RPVSALSPAG LTTLPGTDVA EHGMPGVPGV PVAAGRASGV LPRYGVRLTX

Validated NT Sequence

cannnncgtg agccgaaccc cgtatcgagg taggacgccg gaggctcgcc ctgctgccac tggcaccccc ggtacacctg gcatcccgtg ctcggccacg tcggtccccg ggagtgtggt caggccggcg ggcgacaatg ccgagacagg gcgcgtcgag ggcgcggccc agctcggtgg gaccgatagt tgcccaatcg agtttgcccg gccgacactc gcgagcaccg tgttgccgac gctcgccgca ccgccggcac ccgacgcaga caccccaccg ccgggactgg tcgcggaaac caactcgccg atcccaaagt cgcccggtgt cacctccgcg ggattctcgt cgccgacgtt caaaaggcct aggttgctct cggccccgat ggtcccggca acaaaggact cgacatcctg cgtcacacct accgtggcat atccagcgaa tatggcgtca aggaaggtga agtcctcagg gatgaagctc ggaatcgtca gcgcttgcag cgcttgggtc accgtctcaa tcagcagcga cagtgggtcg gtggcctggc tgaccgcggc ggcctgagcg gtcaggccgg ccgggttggt ggtctgccgc ggcggggaga acggtgtcag cagggccgca gccgctgagg cggtggcgta accgtacatc gcggcggcgt cctgggccca catctcggcg tactgtgcct cggtggccgc gatcgccgca gtgttctggc cgaagaagtt cgtcgcgatc agtgctagca gctgtatccg gttggccgct accaccggtg gcggcagggt cattgcgtat gcttgctcga aggccagcgc tgccgccctg gcttggatcg ctgtttgctg tgtcttttcg gcggtcgtgt acagccaacc gatataggga gcggccgtca ccgccatcga ttccgctgcc ggtccacgcc aatgcaaggc ggccagtccg gacagcaccg atccatatgc ctcggctgtg gtggccaact cggcggccag cgaatcccag ccgcccgcgg cagccaacag cgatcctgcc ccagggccgg tgtacatccg ggcggagttg acttccggtg gtaacagtgc gaaatccata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatt tcgcactgtt accaccggaa gtcaactccg cccggatgta caccggccct ggggcaggat cgctgttggc tgccgcgggc ggctgggatt cgctggccgc cgagttggcc accacagccg aggcatatgg atcggtgctg tccggactgg ccgccttgca ttggcgtgga ccggcagcgg aatcgatggc ggtgacggcc gctccctata tcggttggct gtacacgacc gccgaaaaga cacagcaaac agcgatccaa gccagggcgg cagcgctggc cttcgagcaa gcatacgcaa tgaccctgcc gccaccggtg gtagcggcca accggataca gctgctagca ctgatcgcga cgaacttctt cggccagaac actgcggcga tcgcggccac cgaggcacag tacgccgaga tgtgggccca ggacgccgcc gcgatgtacg gttacgccac cgcctcagcg gctgcggccc tgctgacacc gttctccccg ccgcggcaga ccaccaaccc ggccggcctg accgctcagg ccgccgcggt cagccaggcc accgacccac tgtcgctgct gattgagacg gtgacccaag cgctgcaagc gctgacgatt ccgagcttca tccctgagga cttcaccttc cttgacgcca tattcgctgg atatgccacg gtaggtgtga cgcaggatgt cgagtccttt gttgccggga ccatcggggc cgagagcaac ctaggccttt tgaacgtcgg cgacgagaat cccgcggagg tgacaccggg cgactttggg atcggcgagt tggtttccgc gaccagtccc ggcggtgggg tgtctgcgtc gggtgccggc ggtgcggcga gcgtcggcaa cacggtgctc gcgagtgtcg gccgggcaaa ctcgattggg caactatcgg tcccaccgag ctgggccgcg ccctcgacgc gccctgtctc ggcattgtcg cccgccggcc tgaccacact cccggggacc gacgtggccg agcacgggat gccaggtgta ccgggggtgc cagtggcagc agggcgagcc tccggcgtcc tacctcgata cggggttcgg ctcacggtga tggcccaccc acccgcggca gggtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat