MytuD.18214.g

PPE FAMILY PROTEIN

CENTER ID: MytuD.18214.g
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18214.g.A1.GE34225 full length 1 408
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.3224
RefSeq: YP_177913
TubercuList: Rv2892c
MTB Network Portal: Rv2892c
UniProt: P9WHZ1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDFGVLPPEI NSGRMYAGPG SGPMMAAAAA WDSLAAELGL AAGGYRLAIS ELTGAYWAGP AAASMVAAVT PYVAWLSATA GQAEQAGMQA RAAAAAYELA FAMTVPPPVV VANRALLVAL VATNFFGQNT PAIAATEAQY AEMWAQDAAA MYAYAGSAAI ATELTPFTAA PVTTSPAALA GQAAATVSST VPPLATTAAV PQLLQQLSST SLIPWYSALQ QWLAENLLGL TPDNRMTIVR LLGISYFDEG LLQFEASLAQ QAIPGTPGGA GDSGSSVLDS WGPTIFAGPR ASPSVAGGGA VGGVQTPQPY WYWALDRESI GGSVSAALGK GSSAGSLSVP PDWAARARWA NPAAWRLPGD DVTALRGTAE NALLRGFPMA SAGQSTGGGF VHKYGFRLAV MQRPPFAG
NT Sequence
ttggactttg gggtgttgcc gcccgagatc aactccggtc gaatgtacgc aggtccggga tcggggccca tgatggccgc cgcggcggcc tgggatagtt tggcggctga attgggcttg gccgccggcg gctaccggtt ggctatctcg gagctgaccg gtgcgtactg ggcggggccc gcagcggcgt cgatggtggc cgcggtcacg ccttatgtgg cgtggctcag cgccaccgcc ggtcaggctg aacaggcggg catgcaggcc agagcggccg cggcagccta tgagctggcg tttgcgatga cggtgccccc gccggtggtc gttgccaacc gcgcgctgtt ggtggcgctg gtcgcgacca actttttcgg gcagaacacc ccggcgatcg cggccaccga ggcccaatac gccgagatgt gggcccaaga cgctgccgcc atgtacgcct atgcgggctc cgcggcaatc gccacggagt tgaccccgtt caccgcggcg ccagtgacta ccagccctgc cgcgctggcc ggccaggcgg cggcgacggt atcgtcgacg gtcccaccgc tggccaccac cgccgcggtg ccccaactgt tgcagcaact ctcgtcgacg tcactgatcc cgtggtactc ggcattgcag caatggctcg cggagaatct tctcggtctc acgcctgaca acaggatgac gatcgttcgg ttgctgggca tcagctactt cgacgaaggg ctactgcaat tcgaggcttc gctcgcccag caagcgatcc cgggcacccc gggcggcgcc ggtgactcgg gatcctcggt gcttgacagc tggggtccaa ccatcttcgc cggcccgcgg gcgagcccaa gtgtggcagg cggcggtgct gttggcgggg ttcagacgcc ccagccgtac tggtactggg cactagaccg agaaagcatt ggcggatcgg tatcggccgc tttggggaag gggagctcgg ctggttcatt gtcggtgcca cccgattggg ccgcccgcgc tcgatgggca aacccagcgg cctggcggtt gccgggcgac gatgtcaccg ccctgcgcgg caccgcggaa aacgccctgc ttcggggatt tccgatggcc agcgccggcc agagcaccgg cggcggcttc gttcacaaat acgggttccg cttggcggta atgcagcgtc cgcccttcgc tggatag
Details for MytuD.18214.g.A1.GE34225
HARVESTED ON: 12/19/2011
SEQUENCED ON: 1/3/2012
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 29
PERCENT COVERAGE: 71
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLDFGVLPP EINSGRMYAG PGSGPMMAAA AAWDSLAAEL GLAAGGYRLA IXXLTGAYWA AAXXXXXAXT PXXXWXRXPX VXXXXXXXXG XXGXXXXLXA GXCXXGXPXX XXXXXPXXXX VXXGXXXFFX XXXTPXXXXX PXAXXXPXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXG XXXXXXXXXX XXXXXXLXXX XXXPXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXGXXXXX XXXXXXXXXX XXXXXXX
Validated NT Sequence
ttttnnttna ctttangaag gagntntacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttgg actttggggt gttgccgccc gagatcaact ccggtcgaat gtacgcaggt ccgggatcgg ggcccatgat ggccgccgcg gcggcctggg atagtttggc ggctgaattg ggcttggccg ccggcggcta ccggttggct atctngnngc tgaccggtgc gtactgggcg gcagcgncgn caatnnngnc ngcnnncacg ccttangnnn ngtggntcag gncaccgcng gtnanggnnn annngnnngn natnnngggn nngannggnc nnnngnnann cctatnagct ggnntttgcn atnnnggtnc cccccnncng gnggnnnntn gcnaaccgnn ncncngntnn gtggngnntg gnngnnncca nttttttngg ngnngnanna ccccggnnnn tngnngnnna ccnngggccn nannaanncc nnnnannngg nnncannnnn ntnnnnnnna tnnnngggnn ncnnnnnnnn tnnnnnnggn nnnnannnnn nnnnccgngn nnnnnnnnnt annnnnnnnn nnnnnnnngn ngnnnangng gngngnaggn tnnnnnnann nnnnncnnnn ngnnnncnnn nnnnnnngnn nnnnnnnnct nnnnnnnann nnncnnnnnc ccnngnnnnn nnnnnttnnn nannnnnnnn nnnnannnnn nnnnnnnnnn nnnnnnnnan nnnnnnnnnn ntnnnnnnnn nnnnnnnnnn nnngnannnn tnnnnnnnnn nntnnnnccn nnnnnnnnnn cnnggnnnnn cnngnnannn nnggnnnnnn nnngnnnnnn nncnnncnnn gnnnnncnna ntnnnnnann nnnnnnnnan nncnnnnnnn tnnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLDFGVLPP EINSGRMYAG PGSGPMMAAA AAWDSLAAEL GLAAGGYRLA ISELTGAYWA GPAAASMVAA VTPYVAWLSA TAGQAEQAGM QARAAAAAYE LAFAMTVPPP VVVANRALLV ALVATNFFGQ NTPAIAATEA QYAEMWAQDA AAMYAYAGSA AIATELTPFT AAPVTTSPAA LAGQAAATVS STVPPLATTA AVPQLLQQLS STSLIPWYSA LQQWLAENLL GLTPDNRMTI VRLLGISYFD EGLLQFEASL AQQAIPGTPG GAGDSGSSVL DSWGPTIFAG PRASPSVAGG GAVGGVQTPQ PYWYWALDRE SIGGSVSAAL GKGSSAGSLS VPPDWAARAR WANPAAWRLP GDDVTALRGT AENALLRGFP MASAGQSTGG GFVHKYGFRL AVMQRPPFAG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttggacttt ggggtgttgc cgcccgagat caactccggt cgaatgtacg caggtccggg atcggggccc atgatggccg ccgcggcggc ctgggatagt ttggcggctg aattgggctt ggccgccggc ggctaccggt tggctatctc ggagctgacc ggtgcgtact gggcggggcc cgcagcggcg tcgatggtgg ccgcggtcac gccttatgtg gcgtggctca gcgccaccgc cggtcaggct gaacaggcgg gcatgcaggc cagagcggcc gcggcagcct atgagctggc gtttgcgatg acggtgcccc cgccggtggt cgttgccaac cgcgcgctgt tggtggcgct ggtcgcgacc aactttttcg ggcagaacac cccggcgatc gcggccaccg aggcccaata cgccgagatg tgggcccaag acgctgccgc catgtacgcc tatgcgggct ccgcggcaat cgccacggag ttgaccccgt tcaccgcggc gccagtgact accagccctg ccgcgctggc cggccaggcg gcggcgacgg tatcgtcgac ggtcccaccg ctggccacca ccgccgcggt gccccaactg ttgcagcaac tctcgtcgac gtcactgatc ccgtggtact cggcattgca gcaatggctc gcggagaatc ttctcggtct cacgcctgac aacaggatga cgatcgttcg gttgctgggc atcagctact tcgacgaagg gctactgcaa ttcgaggctt cgctcgccca gcaagcgatc ccgggcaccc cgggcggcgc cggtgactcg ggatcctcgg tgcttgacag ctggggtcca accatcttcg ccggcccgcg ggcgagccca agtgtggcag gcggcggtgc tgttggcggg gttcagacgc cccagccgta ctggtactgg gcactagacc gagaaagcat tggcggatcg gtatcggccg ctttggggaa ggggagctcg gctggttcat tgtcggtgcc acccgattgg gccgcccgcg ctcgatgggc aaacccagcg gcctggcggt tgccgggcga cgatgtcacc gccctgcgcg gcaccgcgga aaacgccctg cttcggggat ttccgatggc cagcgccggc cagagcaccg gcggcggctt cgttcacaaa tacgggttcc gcttggcggt aatgcagcgt ccgcccttcg ctggataaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa