MytuD.18214.e

PPE FAMILY PROTEIN (Rv1808)

CENTER ID: MytuD.18214.e
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18214.e.A1.GE34384 full length 1 409
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.2020
RefSeq: YP_177844
TubercuList: Rv1808
MTB Network Portal: Rv1808
UniProt: P9WI05

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDFGALPPEI NSGRMYAGPG SGPLLAAAAA WDALAAELYS AAASYGSTIE GLTVAPWMGP SSITMAAAVA PYVAWISVTA GQAEQAGAQA KIAAGVYETA FAATVPPPVI EANRALLMSL VATNIFGQNT PAIAATEAHY AEMWAQDAAA MYGYAGSSAT ASQLAPFSEP PQTTNPSATA AQSAVVAQAA GAAASSDITA QLSQLISLLP STLQSLATTA TATSASAGWD TVLQSITTIL ANLTGPYSII GLGAIPGGWW LTFGQILGLA QNAPGVAALL GPKAAAGALS PLAPLRGGYI GDITPLGGGA TGGIARAIYV GSLSVPQGWA EAAPVMRAVA SVLPGTGAAP ALAAEAPGAL FGEMALSSLA GRALAGTAVR SGAGAARVAG GSVTEDVAST TTIIVIPAD
NT Sequence
ttggatttcg gggcgctacc accggagatt aattcggggc gaatgtacgc gggtccggga tccggaccgt tgctggccgc cgcagcggcc tgggatgcgc tagccgccga gttgtactcc gcggcggcgt cctatggctc aacgattgag ggcctcaccg tagcaccgtg gatgggtccc tcctcgatca cgatggccgc cgcggtcgct ccatatgtgg cgtggattag cgtcaccgcc ggccaggccg aacaggcagg ggcccaggcc aagatcgctg cgggcgttta tgagacggca tttgcggcaa cggtgccgcc accggtaatc gaggccaacc gcgctttgtt aatgtcgctg gtcgccacga acatcttcgg gcagaacaca ccggcgatcg cggccaccga ggcccactac gcggagatgt gggcgcaaga tgcggccgcg atgtatggct atgccggctc gtcggccact gcgtcgcagt tggcgccgtt cagcgagccg ccgcaaacga ccaatccgtc ggcaacggcc gctcaatcag ccgtcgtcgc ccaggccgcc ggcgccgcgg ccagctctga catcacagcg cagctgtccc agttgatcag cctgctaccc agcaccttgc aaagcctggc gacaacagcg accgcgacgt cggccagcgc tggttgggac accgtcctgc aaagcatcac cactatcttg gcgaacctca ctgggccgta cagcatcatc gggctgggcg ctatacctgg cggctggtgg ctgacgttcg gccagatcct cggcctagcc caaaacgccc caggtgtggc cgccctactg ggcccgaaag ccgccgccgg cgcgttgtcg ccattggcgc cgctacgggg cgggtatatc ggagatatca cgcctctcgg tggtggggcc acagggggca tcgcccgtgc gatctacgtc gggtcgctct cggtcccgca gggctgggcc gaagccgcac cggtgatgag ggcggtcgca tcggtattgc cgggcaccgg cgccgccccc gccctggccg ccgaggcacc aggtgccttg ttcggcgaga tggccctgtc gagtctggcc ggacgcgcgc tggcaggaac cgcggtgcgc tctggtgccg gagctgctcg cgtcgcaggc ggttccgtca ccgaagacgt cgccagcacg accaccatca tcgtcatacc cgcggactga
Details for MytuD.18214.e.A1.GE34384
HARVESTED ON: 1/10/2012
SEQUENCED ON: 1/13/2012
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 77
PERCENT COVERAGE: 88
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLDFGALPP EINSGRMYAG PGSGPLLAAA AAWDALAAEL YSAAASYGST IEGLTVAPWM GPSSITMAAA VAPYVAWISV TAGQAEQAGA QAKIAAGVYE TAFAATVPPP VIEANRALLM SLVATNIFGQ NTPAIAATEA HYAEMWAQDA AAMYGYAGSS ATASQLAPFS EPPQTTNPSA TAAQSAVVAQ AAGAAASSDI TAQLSQLISL LPSTLQSLAT TATATSASAG WDTVLQSITT ILANLTGXYS IIGXGAIPGG WWLTFXPDPX XXPKTPXXGP YXXESXXGAX SPXXXSGXXI XXXXXXGXGX XGHXXAIYXX XXXXXXXXXX XXXXXXXXXX PXXXXXXXXX XXXXXXXXXX X
Validated NT Sequence
ttgtttanct ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgttggat ttcggggcgc taccaccgga gattaattcg gggcgaatgt acgcgggtcc gggatccgga ccgttgctgg ccgccgcagc ggcctgggat gcgctagccg ccgagttgta ctccgcggcg gcgtcctatg gctcaacgat tgagggcctc accgtagcac cgtggatggg tccctcctcg atcacgatgg ccgccgcggt cgctccatat gtggcgtgga ttagcgtcac cgccggccag gccgaacagg caggggccca ggccaagatc gctgcgggcg tttatgagac ggcatttgcg gcaacggtgc cgccaccggt aatcgaggcc aaccgcgctt tgttaatgtc gctggtcgcc acgaacatct tcgggcagaa cacaccggcg atcgcggcca ccgaggccca ctacgcggag atgtgggcgc aagatgcggc cgcgatgtat ggctatgccg gctcgtcggc cactgcgtcg cagttggcgc cgttcagcga gccgccgcaa acgaccaatc cgtcggcaac ggccgctcaa tcagccgtcg tcgcccaggc cgccggcgcc gcggccagct ctgacatcac agcgcagctg tcccagttga tcagcctgct acccagcacc ttgcaaagcc tggcgacaac agcgaccgcg acgtcngcca gcgctggttg ggacaccgtc ctgcaaagca tcaccactat cttggcgaac ctcactgggn cgtacagcat catcgggntg ggcgctatac ctggcggctg gtggctgacg ttcngnccag atcctcngnc ntanccnaaa acgccccnnn gtggccccta ctnnnccgaa agccnnnccg gcgcgtngtc gccnttnnnc nnctcgggnn ggnatatcnn nnntatnann nnnntnggng ntggnnnnnn ggggcatcnn cnngcgatct acntcnggnc gnnntngnnc cncnnnnngn nnncnnnatg nnnnnnnnat nnnnnnnnnn nnnnnnnccc nnnnnnncnn nnnnnnnnnn nnngnnnann nnnnnnnnnn nnngnnnnng nnnnnnnnnn nc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLDFGALPP EINSGRMYAG PGSGPLLAAA AAWDALAAEL YSAAASYGST IEGLTVAPWM GPSSITMAAA VAPYVAWISV TAGQAEQAGA QAKIAAGVYE TAFAATVPPP VIEANRALLM SLVATNIFGQ NTPAIAATEA HYAEMWAQDA AAMYGYAGSS ATASQLAPFS EPPQTTNPSA TAAQSAVVAQ AAGAAASSDI TAQLSQLISL LPSTLQSLAT TATATSASAG WDTVLQSITT ILANLTGPYS IIGLGAIPGG WWLTFGQILG LAQNAPGVAA LLGPKAAAGA LSPLAPLRGG YIGDITPLGG GATGGIARAI YVGSLSVPQG WAEAAPVMRA VASVLPGTGA APALAAEAPG ALFGEMALSS LAGRALAGTA VRSGAGAARV AGGSVTEDVA STTTIIVIPA D
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttggatttc ggggcgctac caccggagat taattcgggg cgaatgtacg cgggtccggg atccggaccg ttgctggccg ccgcagcggc ctgggatgcg ctagccgccg agttgtactc cgcggcggcg tcctatggct caacgattga gggcctcacc gtagcaccgt ggatgggtcc ctcctcgatc acgatggccg ccgcggtcgc tccatatgtg gcgtggatta gcgtcaccgc cggccaggcc gaacaggcag gggcccaggc caagatcgct gcgggcgttt atgagacggc atttgcggca acggtgccgc caccggtaat cgaggccaac cgcgctttgt taatgtcgct ggtcgccacg aacatcttcg ggcagaacac accggcgatc gcggccaccg aggcccacta cgcggagatg tgggcgcaag atgcggccgc gatgtatggc tatgccggct cgtcggccac tgcgtcgcag ttggcgccgt tcagcgagcc gccgcaaacg accaatccgt cggcaacggc cgctcaatca gccgtcgtcg cccaggccgc cggcgccgcg gccagctctg acatcacagc gcagctgtcc cagttgatca gcctgctacc cagcaccttg caaagcctgg cgacaacagc gaccgcgacg tcggccagcg ctggttggga caccgtcctg caaagcatca ccactatctt ggcgaacctc actgggccgt acagcatcat cgggctgggc gctatacctg gcggctggtg gctgacgttc ggccagatcc tcggcctagc ccaaaacgcc ccaggtgtgg ccgccctact gggcccgaaa gccgccgccg gcgcgttgtc gccattggcg ccgctacggg gcgggtatat cggagatatc acgcctctcg gtggtggggc cacagggggc atcgcccgtg cgatctacgt cgggtcgctc tcggtcccgc agggctgggc cgaagccgca ccggtgatga gggcggtcgc atcggtattg ccgggcaccg gcgccgcccc cgccctggcc gccgaggcac caggtgcctt gttcggcgag atggccctgt cgagtctggc cggacgcgcg ctggcaggaa ccgcggtgcg ctctggtgcc ggagctgctc gcgtcgcagg cggttccgtc accgaagacg tcgccagcac gaccaccatc atcgtcatac ccgcggacta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a