MytuD.18214.d

PPE FAMILY PROTEIN

CENTER ID: MytuD.18214.d
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18214.d.A1.GE34221 full length 1 463
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.2014
RefSeq: YP_177841
TubercuList: Rv1802
MTB Network Portal: Rv1802
UniProt: P9WI07

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDFGVLPPEI NSGRMYAGPG SGPMLAAAAA WDGLATELQS TAADYGSVIS VLTGVWSGQS SGTMAAAAAP YVAWMSATAA LAREAAAQAS AAAAAYEAAF AATVPPPVVA ANRAELAVLA ATNIFGQNTG AIAAAEARYA EMWAQDAAAM YGYAGSSSVA TQVTPFAAPP PTTNAAGLAT QGVAVAQAVG ASAGNARSLV SEVLEFLATA GTNYNKTVAS LMNAVTGVPY ASSVYNSMLG LGFAESKMVL PANDTVISTI FGMVQFQKFF NPVTPFNPDL IPKSALGAGL GLRSAISSGL GSTAPAISAG ASQAGSVGGM SVPPSWAAAT PAIRTVAAVF SSTGLQAVPA AAISEGSLLS QMALASVAGG ALGGAAARAT GGFLGGGRVT AVKKSLKDSD SPDKLRRVVA HMMEKPESVQ HWHTDEDGLD DLLAELKKKP GIHAVHMAGG NKAEIAPTIS ESG
NT Sequence
atggatttcg gggtgttacc accggagatc aattccgggc gcatgtatgc cggtcccggg tcgggtccga tgctggccgc ggcagcggcc tgggacgggc tggccaccga attacagtcc acggcggccg actatggctc ggtgatctcg gttctgaccg gcgtgtggtc gggacagtcg tcggggacca tggcggctgc ggccgcaccg tatgtggcgt ggatgtcggc cacggcggcg ctcgctcggg aagcggccgc ccaggccagc gcggcagcgg cggcctacga ggcagcgttt gcagccacgg tgccgccgcc ggtcgtcgcg gccaaccgcg ccgagctggc ggtgttggcg gcgaccaaca ttttcggtca gaacaccggt gcgatcgcgg ccgccgaagc ccgctatgcg gaaatgtggg cgcaagacgc agccgcgatg tatggctatg ccggctcgtc gtcggtggcg acccaggtga cgccatttgc tgcaccgccg ccgaccacca acgcggccgg actggccacc caaggcgttg cggttgccca ggctgtcggc gcgtcggccg gcaacgcgcg ctcactggtg tccgaggtgc tggaattcct ggcaacggcc gggacgaact acaacaagac ggtggccagc ctgatgaacg cggtcaccgg ggtgccgtac gcatcttcgg tgtataacag catgctcggg cttggcttcg ctgagtcaaa aatggtcctg ccggctaacg acaccgtaat atcgaccatc ttcggcatgg tgcagttcca gaagttcttc aatccggtga cgcccttcaa tcccgatttg atcccgaaat ctgctctagg ggccgggctt ggcctgcggt ctgcgatctc gagtggtctg ggctcgaccg cgccagcgat atcggcgggt gcgagccagg ccggctcggt cggggggatg tcggtgccgc cgagctgggc agcggccacc ccggcgatcc ggacggttgc cgctgtgttc tcgagcaccg gacttcaggc tgtcccggcg gccgcaatta gcgagggcag tctgctcagc cagatggccc tggcgagtgt ggccggaggg gcccttggcg gcgccgctgc acgcgccact ggtggtttcc tcggcggagg ccgagtcacc gcggtcaaga aatctctcaa ggacagcgac ttaccggaca agctgcggcg ggtggtcgcg cacatgatgg agaagcccga atcggtgcag cactggcaca ccgacgagga cgggctcgat gatctactcg cggaattgaa gaagaaaccg ggcatccacg ccgtgcacat ggccggcggc aacaaggctg aaattgcacc gacgatatca gaatcgggct ag
Details for MytuD.18214.d.A1.GE34221
HARVESTED ON: 12/19/2011
SEQUENCED ON: 12/28/2011
EXPECTED MW: 48kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 82
PERCENT COVERAGE: 74
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDFGVLPPE INSGRMYAGP GSGPMLAAAA AWDGLATELQ STAADYGSVI SVLTGVWSGQ SSGTMAAAAA PYVAWMSATA ALAREAAAQA SAAAAAYEAA FAATVPPPVV AANRAELAVL AATNIFGQNT GAIAAAEXRY AEMWAQDAAA MYGYAGSSSV ATQVTPFAAP PPTTNAAGLA TQGVAVAQAV GASAGNARSL VSXVLEFLAT AGTNYNKTVA SLMNAVTGVP YASSVYNSML GLGFAXSKMV LPANDTVIST IFGMVQFXKF FXXGDALQSR FDPEICSRGX XXXXXCDLXX XGXDXXXYXX XXXXGGXXXX XXXXXXXXXX XXXXXXXXXX X
Validated NT Sequence
attttnttta ctttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggatt tcggggtgtt accaccggag atcaattccg ggcgcatgta tgccggtccc gggtcgggtc cgatgctggc cgcggcagcg gcctgggacg ggctggccac cgaattacag tccacggcgg ccgactatgg ctcggtgatc tcggttctga ccggcgtgtg gtcgggacag tcgtcgggga ccatggcggc tgcggccgca ccgtatgtgg cgtggatgtc ggccacggcg gcgctcgctc gggaagcggc cgcccaggcc agcgcggcag cggcggccta cgaggcagcg tttgcagcca cggtgccgcc gccggtcgtc gcggccaacc gcgccgagct ggcggtgttg gcggcgacca acattttcgg tcagaacacc ggtgcgatcg cggccgccga ancccgctat gcggaaatgt gggcgcaaga cgcagccgcg atgtatggct atgccggctc gtcgtcggtg gcgacccagg tgacgccatt tgctgcaccg ccgccgacca ccaacgcggc cggactggcc acccaaggcg ttgcggttgc ccaggctgtc ggcgcgtcgg ccggcaacgc gcgctcactg gtgtccgang tgctggaatt cctggcaacg gccgggacga actacaacaa gacggtggcc agcctgatga acgcggtcac cggggtgccg tacgcatctt cngtgtataa cagcatgctc gggcttggct tcgctgantc aaaaatggtc ctgccggcta acgacaccgt aatatcgacc atcttcggca tggtgcagtt ccngaagttc ttcaannccg gtgacgccct tcaatcccga tttgatcccg aaatctgctc taggggnnng gnttnnnntn cnntctgcga tctcnnnnng nctgggntcg acnnncngcn ntatngnngg nncnngntnn nngggggnng tcnnnncnnn anntnggnan nnnnnnnnnn nnannnnnng nnnnnntnnn tncnnnnnnn nnnnnttnnn nnnnnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDFGVLPPE INSGRMYAGP GSGPMLAAAA AWDGLATELQ STAADYGSVI SVLTGVWSGQ SSGTMAAAAA PYVAWMSATA ALAREAAAQA SAAAAAYEAA FAATVPPPVV AANRAELAVL AATNIFGQNT GAIAAAEARY AEMWAQDAAA MYGYAGSSSV ATQVTPFAAP PPTTNAAGLA TQGVAVAQAV GASAGNARSL VSEVLEFLAT AGTNYNKTVA SLMNAVTGVP YASSVYNSML GLGFAESKMV LPANDTVIST IFGMVQFQKF FNPVTPFNPD LIPKSALGAG LGLRSAISSG LGSTAPAISA GASQAGSVGG MSVPPSWAAA TPAIRTVAAV FSSTGLQAVP AAAISEGSLL SQMALASVAG GALGGAAARA TGGFLGGGRV TAVKKSLKDS DLPDKLRRVV AHMMEKPESV QHWHTDEDGL DDLLAELKKK PGIHAVHMAG GNKAEIAPTI SESG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatttcggg gtgttaccac cggagatcaa ttccgggcgc atgtatgccg gtcccgggtc gggtccgatg ctggccgcgg cagcggcctg ggacgggctg gccaccgaat tacagtccac ggcggccgac tatggctcgg tgatctcggt tctgaccggc gtgtggtcgg gacagtcgtc ggggaccatg gcggctgcgg ccgcaccgta tgtggcgtgg atgtcggcca cggcggcgct cgctcgggaa gcggccgccc aggccagcgc ggcagcggcg gcctacgagg cagcgtttgc agccacggtg ccgccgccgg tcgtcgcggc caaccgcgcc gagctggcgg tgttggcggc gaccaacatt ttcggtcaga acaccggtgc gatcgcggcc gccgaagccc gctatgcgga aatgtgggcg caagacgcag ccgcgatgta tggctatgcc ggctcgtcgt cggtggcgac ccaggtgacg ccatttgctg caccgccgcc gaccaccaac gcggccggac tggccaccca aggcgttgcg gttgcccagg ctgtcggcgc gtcggccggc aacgcgcgct cactggtgtc cgaggtgctg gaattcctgg caacggccgg gacgaactac aacaagacgg tggccagcct gatgaacgcg gtcaccgggg tgccgtacgc atcttcggtg tataacagca tgctcgggct tggcttcgct gagtcaaaaa tggtcctgcc ggctaacgac accgtaatat cgaccatctt cggcatggtg cagttccaga agttcttcaa tccggtgacg cccttcaatc ccgatttgat cccgaaatct gctctagggg ccgggcttgg cctgcggtct gcgatctcga gtggtctggg ctcgaccgcg ccagcgatat cggcgggtgc gagccaggcc ggctcggtcg gggggatgtc ggtgccgccg agctgggcag cggccacccc ggcgatccgg acggttgccg ctgtgttctc gagcaccgga cttcaggctg tcccggcggc cgcaattagc gagggcagtc tgctcagcca gatggccctg gcgagtgtgg ccggaggggc ccttggcggc gccgctgcac gcgccactgg tggtttcctc ggcggaggcc gagtcaccgc ggtcaagaaa tctctcaagg acagcgactt accggacaag ctgcggcggg tggtcgcgca catgatggag aagcccgaat cggtgcagca ctggcacacc gacgaggacg ggctcgatga tctactcgcg gaattgaaga agaaaccggg catccacgcc gtgcacatgg ccggcggcaa caaggctgaa attgcaccga cgatatcaga atcgggctaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa