MytuD.18214.c

PPE FAMILY PROTEIN

CENTER ID: MytuD.18214.c
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18214.c.A1.GE34222 full length 1 385
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.1895
RefSeq: YP_177827
TubercuList: Rv1705c
MTB Network Portal: Rv1705c
UniProt: P9WI19

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDFGALPPEV NSGRMYCGPG SAPMVAAASA WNGLAAELSV AAVGYERVIT TLQTEEWLGP ASTLMVEAVA PYVAWMRATA IQAEQAASQA RAAAAAYETA FAAIVPPPLI AANRARLTSL VTHNVFGQNT ASIAATEAQY AEMWAQDAMA MYGYAGSSAT ATKVTPFAPP PNTTSPSAAA TQLSAVAKAA GTSAGAAQSA IAELIAHLPN TLLGLTSPLS SALTAAATPG WLEWFINWYL PISQLFYNTV GLPYFAIGIG NSLITSWRAL GWIGPEAAEA AAAAPAAVGA AVGGTGPVSA GLGNAATIGK LSLPPNWAGA SPSLAPTVGS ASAPLVSDIV EQPEAGAAGN LLGGMPLAGS GTGTGGAGPR YGFRVTVMSR PPFAG
NT Sequence
atggattttg gggcgttgcc accggaggtg aactccgggc gcatgtattg cggcccgggc tcggcaccga tggttgccgc agcgtccgcg tggaacgggc tggccgccga gctcagtgtg gccgccgtcg gctacgagcg ggtgatcacg acgctgcaaa ccgaagaatg gctgggtccg gcgtcgacgt tgatggtcga agcggtcgcc ccgtatgtgg cgtggatgcg agccaccgcc atccaagctg agcaggcagc cagtcaggcg cgtgcggcgg cggccgctta cgaaaccgcg tttgccgcca ttgtgccccc gccgctgatc gcggccaatc gcgcgcggct cacgtcgctg gtcacgcata acgtgttcgg tcaaaacacc gcatcaatcg cggccaccga agctcaatac gctgaaatgt gggcccaaga cgccatggcc atgtacggtt acgcgggctc ctcggcgacc gcgaccaaag tgacgccgtt tgccccgccg ccgaacacca ccagcccatc cgccgcggcg acgcagttgt ccgcagtcgc caaagccgcc ggcacctcag ccggcgcggc gcagagcgca atagcggagc tgatcgccca cttgcccaac acgctgctgg gcctcacctc gcccctgtcg tccgcgttga ccgccgccgc gaccccgggg tggctggaat ggttcatcaa ctggtatctg ccgatatcac agctgtttta caacaccgtg ggtttgccat atttcgccat tggtattggc aacagcctta ttacatcgtg gcgggcgctg ggatggatcg gtcccgaggc cgccgaagct gccgccgcgg cgccggcggc cgtcggcgcc gcggttggcg gtaccggacc cgtatccgcg ggtctaggca acgcggccac catcggcaag ttatcgctgc cgcccaactg ggcaggggcc agtccgagcc tagccccgac cgtgggttcg gcctccgcac ccctggtcag cgatatcgtc gagcaaccgg aggccggtgc ggcgggaaac ctgttgggcg gcatgccgct agccggttcg ggcaccggta cggggggtgc gggtccccgc tacgggttcc gggttacggt gatgtcccgg ccgccgtttg ccggataa
Details for MytuD.18214.c.A1.GE34222
HARVESTED ON: 12/19/2011
SEQUENCED ON: 1/3/2012
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 19
Validated AA Sequence
MAHHHHHHMG XLEAQTQGPG SMDFGALPPE VNSGRMYCGP GSAPMVAAAS AWNGLAAELS VAAVGYERVI TTLQTEEWL
Validated NT Sequence
tntaccatgg ctcatcacca tcaccatcat atgggtnccc tggaagctca gacccagggt cctggttcga tggattttgg ggcgttgcca ccggaggtga actccgggcg catgtattgc ggcccgggct cggcaccgat ggttgccgca gcgtccgcgt ggaacgggct ggccgccgag ctcagtgtgg ccgccgtcgg ctacgagcgg gtgatcacga cgctgcaaac cgaagaatgg ctgg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDFGALPPE VNSGRMYCGP GSAPMVAAAS AWNGLAAELS VAAVGYERVI TTLQTEEWLG PASTLMVEAV APYVAWMRAT AIQAEQAASQ ARAAAAAYET AFAAIVPPPL IAANRARLTS LVTHNVFGQN TASIAATEAQ YAEMWAQDAM AMYGYAGSSA TATKVTPFAP PPNTTSPSAA ATQLSAVAKA AGTSAGAAQS AIAELIAHLP NTLLGLTSPL SSALTAAATP GWLEWFINWY LPISQLFYNT VGLPYFAIGI GNSLITSWRA LGWIGPEAAE AAAAAPAAVG AAVGGTGPVS AGLGNAATIG KLSLPPNWAG ASPSLAPTVG SASAPLVSDI VEQPEAGAAG NLLGGMPLAG SGTGTGGAGP RYGFRVTVMS RPPFAG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggattttggg gcgttgccac cggaggtgaa ctccgggcgc atgtattgcg gcccgggctc ggcaccgatg gttgccgcag cgtccgcgtg gaacgggctg gccgccgagc tcagtgtggc cgccgtcggc tacgagcggg tgatcacgac gctgcaaacc gaagaatggc tgggtccggc gtcgacgttg atggtcgaag cggtcgcccc gtatgtggcg tggatgcgag ccaccgccat ccaagctgag caggcagcca gtcaggcgcg tgcggcggcg gccgcttacg aaaccgcgtt tgccgccatt gtgcccccgc cgctgatcgc ggccaatcgc gcgcggctca cgtcgctggt cacgcataac gtgttcggtc aaaacaccgc atcaatcgcg gccaccgaag ctcaatacgc tgaaatgtgg gcccaagacg ccatggccat gtacggttac gcgggctcct cggcgaccgc gaccaaagtg acgccgtttg ccccgccgcc gaacaccacc agcccatccg ccgcggcgac gcagttgtcc gcagtcgcca aagccgccgg cacctcagcc ggcgcggcgc agagcgcaat agcggagctg atcgcccact tgcccaacac gctgctgggc ctcacctcgc ccctgtcgtc cgcgttgacc gccgccgcga ccccggggtg gctggaatgg ttcatcaact ggtatctgcc gatatcacag ctgttttaca acaccgtggg tttgccatat ttcgccattg gtattggcaa cagccttatt acatcgtggc gggcgctggg atggatcggt cccgaggccg ccgaagctgc cgccgcggcg ccggcggccg tcggcgccgc ggttggcggt accggacccg tatccgcggg tctaggcaac gcggccacca tcggcaagtt atcgctgccg cccaactggg caggggccag tccgagccta gccccgaccg tgggttcggc ctccgcaccc ctggtcagcg atatcgtcga gcaaccggag gccggtgcgg cgggaaacct gttgggcggc atgccgctag ccggttcggg caccggtacg gggggtgcgg gtccccgcta cgggttccgg gttacggtga tgtcccggcc gccgtttgcc ggataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa