MytuD.18214.b

PPE FAMILY PROTEIN

CENTER ID: MytuD.18214.b
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18214.b.A1.GE34223 full length 1 391
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.1157
RefSeq: YP_177778
TubercuList: Rv1039c
MTB Network Portal: Rv1039c
UniProt: P9WI31

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDFGALPPEI NSARMYAGAG AGPMMAAGAA WNGLAAELGT TAASYESVIT RLTTESWMGP ASMAMVAAAQ PYLAWLTYTA EAAAHAGSQA MASAAAYEAA YAMTVPPEVV AANRALLAAL VATNVLGINT PAIMATEALY AEMWAQDALA MYGYAAASGA AGMLQPLSPP SQTTNPGGLA AQSAAVGSAA ATAAVNQVSV ADLISSLPNA VSGLASPVTS VLDSTGLSGI IADIDALLAT PFVANIINSA VNTAAWYVNA AIPTAIFLAN ALNSGAPVAI AEGAIEAAEG AASAAAAGLA DSVTPAGLGA SLGEATLVGR LSVPAAWSTA APATTAGATA LEGSGWTVAA EEAGPVTGMM PGMASAAKGT GAYAGPRYGF KPTVMPKQVV V
NT Sequence
atggatttcg gagctttacc ccctgagatc aactccgcac gcatgtacgc cggcgcgggt gcaggaccga tgatggccgc cggggccgca tggaacggcc tggccgccga gttgggtacg acggccgcgt cgtatgagtc ggtgatcacc cggctgacca ccgagtcgtg gatgggtccg gcctcgatgg cgatggtcgc cgcagcccag ccctatctgg cttggttgac ctacaccgcc gaagccgctg cgcatgccgg ctcgcaggcc atggcgtcgg cggccgccta cgaggcggcc tatgcgatga cagtgccgcc ggaggtggtc gcggccaacc gggcgctgct ggcggccctg gtcgcgacga acgtcctggg gatcaacaca ccggcaatca tggcgaccga agccctctat gccgagatgt gggctcagga cgctctggct atgtacggct acgcggccgc ttcgggagcc gccgggatgc tgcaaccgtt aagcccgccg tcgcagacca ccaacccggg cgggctggcc gcccagtccg ccgcggtcgg ctcggctgcc gccaccgccg ccgtcaacca ggtgagcgta gcggacctga tcagtagcct gcccaacgcg gtgagtgggc tcgcctcccc agtcacatcg gttctcgact cgacggggct gagcggaatc attgccgaca tcgacgccct gctcgcgacc ccgttcgtgg caaacatcat caacagcgca gtcaacaccg ccgcttggta tgtcaacgcc gccatcccca ccgcgatatt cctagcaaat gccctgaaca gtggggcgcc ggtagcgatc gccgaaggcg ccatcgaggc tgccgagggt gccgccagtg cggccgccgc ggggttggcg gactcggtga cgccagcggg gctcggcgca agtttaggcg aggccaccct ggtcggccgg ctgtcagtgc cggcggcctg gtctacggcc gcaccggcga caaccgccgg cgccacagcg ctcgaaggca gcggctggac cgtcgccgcc gaagaagccg gcccagttac cgggatgatg ccgggaatgg cctcggccgc caagggcacc ggtgcctatg ccgggccgcg gtacggattc aagcccactg tcatgcccaa acaggtcgtc gtgtga
Details for MytuD.18214.b.A1.GE34223
HARVESTED ON: 12/19/2011
SEQUENCED ON: 1/3/2012
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 37
PERCENT COVERAGE: 80
Validated AA Sequence
MAHHHHHHMG XLEAQTQGPG SMDFGALPPE INSARMYAGA GAGPMMAAGA AWNGLAAELG TTAASYESVI TRLTTESWMG PASMAMVAAA QPYLAWLTYT AEAAAHAGSX XXGVGGRLRG GLCXDXXAXX XXXPXRXLLG XXXXXXXXXD XXTGXXXXTX XXXXXXGGXX XXXXXXXXTX XXSXXXXXXX TXXXXXXXXX NPGGXXXXXX XXXGXXXXXX XXXXXXXXXL XXXXPXXXXX XXXPXXXXXX XXXXXXXEXX XXXGXXXXXX XXXXXXXXXX XXXXXXXXXX XXXSGXXXXX XXXXXXXXXX PPXXXXXXXX XXXXXXXXXX XXX
Validated NT Sequence
tnnnnnnnat tttnnttaac tttaagaagg agntntacca tggctcatca ccatcaccat catatgggtn ccctggaagc tcagacccag ggtcctggtt cgatggattt cggagcttta ccccctgaga tcaactccgc acgcatgtac gccggcgcgg gtgcaggacc gatgatggcc gccggggccg catggaacgg cctggccgcc gagttgggta cgacggccgc gtcgtatgag tcggtgatca cccggctgac caccgagtcg tggatgggtc cggcctcgat ggcgatggtc gccgcagccc agccctatct ggcttggttg acctacaccg ccgaagccgc tgcgcatgcn ggctcgcang nnnatggcgt cggcggccgc ctacgaggng gcctatgcna tgacngtncc gccnnnnnnn ggtnncnncc nanccgggnn ctnctggggg nnnnnnnnnc nannaanntn cnnnnggatn aananaccgg ncantnntng nnnacngann cccnnnnnnn nnnnganggg ggnnccngnn nnnnnnnnnn nnnnntnnnn gntacccnnn ccncttcgnn nnnnncnngn nnnnnnnnna ccnntnannc ccncnnnnnn nnnannanna acccgggngg ncnggncnnc cnnnnccncc nnnnnnnggg ttnggnnnnc nnccnncnnc nntnnannng gnnnancnnn nnnnnnctna nnngnnnntg nccaannnng nnnnngnggg nnnnnnnncc cnnnnnntnn nnntnnnngg nnnnannnna annnttnnnn gaanncnnnn nnnnnnnngg gnaannntan naannnnncn gnnnannnnn nnnctngnnn gntnannnnn ncntnnccnn ccncgannnn cnngnnnaat nncnnancag tggnnnnnnn nnnnnnnncn nnnnannnnn cnnnnnncnn nnnntnnnnc cnccnnnnnn nnnnnnnnnn nnnnncnntn nnnnnnnntn nngnnanncn nncnnntnnn nnnnnnnnng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDFGALPPE INSARMYAGA GAGPMMAAGA AWNGLAAELG TTAASYESVI TRLTTESWMG PASMAMVAAA QPYLAWLTYT AEAAAHAGSQ AMASAAAYEA AYAMTVPPEV VAANRALLAA LVATNVLGIN TPAIMATEAL YAEMWAQDAL AMYGYAAASG AAGMLQPLSP PSQTTNPGGL AAQSAAVGSA AATAAVNQVS VADLISSLPN AVSGLASPVT SVLDSTGLSG IIADIDALLA TPFVANIINS AVNTAAWYVN AAIPTAIFLA NALNSGAPVA IAEGAIEAAE GAASAAAAGL ADSVTPAGLG ASLGEATLVG RLSVPAAWST AAPATTAGAT ALEGSGWTVA AEEAGPVTGM MPGMASAAKG TGAYAGPRYG FKPTVMPKQV VV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatttcgga gctttacccc ctgagatcaa ctccgcacgc atgtacgccg gcgcgggtgc aggaccgatg atggccgccg gggccgcatg gaacggcctg gccgccgagt tgggtacgac ggccgcgtcg tatgagtcgg tgatcacccg gctgaccacc gagtcgtgga tgggtccggc ctcgatggcg atggtcgccg cagcccagcc ctatctggct tggttgacct acaccgccga agccgctgcg catgccggct cgcaggccat ggcgtcggcg gccgcctacg aggcggccta tgcgatgaca gtgccgccgg aggtggtcgc ggccaaccgg gcgctgctgg cggccctggt cgcgacgaac gtcctgggga tcaacacacc ggcaatcatg gcgaccgaag ccctctatgc cgagatgtgg gctcaggacg ctctggctat gtacggctac gcggccgctt cgggagccgc cgggatgctg caaccgttaa gcccgccgtc gcagaccacc aacccgggcg ggctggccgc ccagtccgcc gcggtcggct cggctgccgc caccgccgcc gtcaaccagg tgagcgtagc ggacctgatc agtagcctgc ccaacgcggt gagtgggctc gcctccccag tcacatcggt tctcgactcg acggggctga gcggaatcat tgccgacatc gacgccctgc tcgcgacccc gttcgtggca aacatcatca acagcgcagt caacaccgcc gcttggtatg tcaacgccgc catccccacc gcgatattcc tagcaaatgc cctgaacagt ggggcgccgg tagcgatcgc cgaaggcgcc atcgaggctg ccgagggtgc cgccagtgcg gccgccgcgg ggttggcgga ctcggtgacg ccagcggggc tcggcgcaag tttaggcgag gccaccctgg tcggccggct gtcagtgccg gcggcctggt ctacggccgc accggcgaca accgccggcg ccacagcgct cgaaggcagc ggctggaccg tcgccgccga agaagccggc ccagttaccg ggatgatgcc gggaatggcc tcggccgcca agggcaccgg tgcctatgcc gggccgcggt acggattcaa gcccactgtc atgcccaaac aggtcgtcgt gtaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa