SSGCID: Target Page

MytuD.18213.s

PE-PGRS FAMILY PROTEIN

CENTER ID:	MytuD.18213.s
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.18213.s.A2.GE34269	mature protein		16	558

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|83332.12.peg.2214
RefSeq:	YP_177854
TubercuList:	Rv1983
MTB Network Portal:	Rv1983
UniProt:	P9WIF1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSFLVVVPEF LTSAAADVEN IGSTLRAANA AAAASTTALA AAGADEVSAA VAALFARFGQ EYQAVSAQAS AFHQQFVQTL NSASGSYAAA EATIASQLQT AQHDLLGAVN APTETLLGRP LIGDGAPGTA TSPNGGAGGL LYGNGGNGYS ATASGVGGGA GGSAGLIGNG GAGGAGGPNA PGGAGGNGGW LLGNGGIGGP GGASSIPGMS GGAGGTGGAA GLLGWGANGG AGGLGDGVGV DRGTGGAGGR GGLLYGGYGV SGPGGDGRTV PLEIIHVTEP TVHANVNGGP TSTILVDTGS AGLVVSPEDV GGILGVLHMG LPTGLSISGY SGGLYYIFAT YTTTVDFGNG IVTAPTAVNV VLLSIPTSPF AISTYFSALL ADPTTTPFEA YFGAVGVDGV LGVGPNAVGP GPSIPTMALP GDLNQGVLID APAGELVFGP NPLPAPNVEV VGSPITTLYV KIDGGTPIPV PSIIDSGGVT GTIPSYVIGS GTLPANTNIE VYTSPGGDRL YAFNTNDYRP TVISSGLMNT GFLPFRFQPV YIDYSPSGIG TTVFDHPA

NT Sequence

gtgtcatttc tggtcgtggt tcccgagttc ttgacgtccg cggcagcgga tgtggagaac ataggttcca cactgcgcgc ggcgaatgcc gcggctgccg cctcgaccac cgcgcttgcg gccgctggcg ctgatgaggt atcggcggcg gtggcagcgc tgtttgccag gttcggtcag gaatatcaag cggtcagcgc gcaggcgagc gctttccatc aacagttcgt gcagacgctg aactcggcgt caggatcgta tgcggccgcg gaggccacca tcgcgtcaca gttgcagacc gcgcagcacg atctgctggg cgcggtcaat gcaccaaccg aaacgttgtt ggggcgtccg ctaatcggcg acggagcacc cgggacggca acgagtccga atggcggggc gggtgggctg ctgtacggca acggcggcaa cggttattcc gcgacggcgt cgggggtcsg cggcggggcc ggcggttccg cggggttgat cggcaatggc ggcgccgggg gagccggcgg acccaacgcc cccgggggag ccggcggcaa cggtggctgg ctgctcggca acggcgggat cggcgggccc gggggcgcgt cgagcatccc cggcatgagt ggtggagccg gcggaaccgg cggtgccgca ggacttttgg gctggggagc gaacggcgga gccggcggcc tcggtgatgg agtcggtgtc gatcgtggca cgggcggcgc cggaggccgc ggcggcctgt tgtatggcgg atacggcgtc agtgggccag gcggcgacgg cagaaccgtc ccgctggaga taattcatgt cacagagccg acggtacatg ccaacgtcaa cggcggaccg acgtcaacca ttctggtcga caccggatcc gctggtcttg ttgtctcgcc tgaggatgtc gggggaatcc tgggagtgct tcacatgggc ctcccaaccg gattgagcat cagcggttac agcggggggc tgtactacat cttcgccacg tataccacga cggtggactt cgggaatggc atcgtcaccg cgccgaccgc cgttaatgtc gtcctcttgt ccatcccaac gtcccccttc gccatttcga cctacttcag cgccttgctg gccgatccga caacaactcc gttcgaagcc tatttcggtg ccgtcggcgt ggacggcgtt ctgggagttg ggcccaatgc ggtgggacca ggccccagca ttccgacgat ggcgttaccg ggtgacctca accagggagt gctcatcgac gcacccgcag gtgagctcgt gttcggtccc aacccgctac ctgcgcccaa cgtcgaggtc gtcggatcgc cgatcaccac cctgtacgta aagatcgatg gtgggactcc catacccgtc ccctcgatca tcgattccgg tggggtaacg ggaaccatcc cgtcatatgt catcggatcc ggaaccctgc cggcgaacac aaacattgag gtctacacca gccccggcgg tgatcggctc tacgcgttca acacaaacga ttaccgcccg accgtcattt catccggcct gatgaatacc gggttcttgc ccttcagatt ccagccggtg tacatcgact acagccccag cggtataggg acaacagtct ttgatcatcc ggcgtga

Details for MytuD.18213.s.A2.GE34269

HARVESTED ON:	12/19/2011
SEQUENCED ON:	12/28/2011
EXPECTED MW:	54kDa
OBSERVED MW:	54kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	88
PERCENT COVERAGE:	55

Validated AA Sequence

Validated NT Sequence

annnnntttt ntttacttta agaaggagan ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggatgtg gagaacatag gttccacact gcgcgcggcg aatgccgcgg ctgccgcctc gaccaccgcg cttgcggccg ctggcgctga tgaggtatcg gcggcggtgg cagcgctgtt tgccaggttc ggtcaggaat atcaagcggt cagcgcgcag gcgagcgctt tccatcaaca gttcgtgcag acgctgaact cggcgtcagg atcgtatgcg gccgcggagg ccaccatcgc gtcacagttg cagaccgcgc agcacgatct gctgggcgcg gtcaatgcac caaccgaaac gttgttgggg cgtccgctaa tcggcgacgg agcacccggg acggcaacga gtccgaatgg cggggcgggt gggctgctgt acggcaacgg cggcaacggt tattccgcga cggcgtcggg ggtcggcggc ggggccggcg gttccgcggg gttgatcggc aatggcggcg ccgggggagc cggcggaccc aacgcccccg ggggagtcgg cggcaacggt ggctggctgc tcggcaacgg cgggatcggc gggcccgggg gcgcgtcgag catccccggc atgagtggtg gagccggcgg aaccggcggt gccgcaggac ttttgggctg gggagcgaac ggcggagccg gcggcctcgg tgatggagtc ggtgtcgatc gtggcacggg cggcgccnga ngccgcggcg gcctgttgta tggcggatac ggcgtcagtg ggccnggcgg cgacngcaga ancngtcccg ctggagataa ttcatgtcac agagccgacg gtacntgcca acgtcaacgn nggaccgacg tcaaccattc tggncgacnc cngatccgct ggtcttgntg tctcgcctga nnatgtcggg ggaatnntgg nnntgcttnn nntggnnntc ccnnncngna tngagcatca nnnnnnnnnn ngggggnntn nactacntnn tnnnnncnnn nnnngnnnnn nnnnnnnnat cnnnncnnnn nnacnnnnnn nnnnncnncn nnnnnnnnnn nnnnnnnnnt nnnnnnttt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMADVENIGS TLRAANAAAA ASTTALAAAG ADEVSAAVAA LFARFGQEYQ AVSAQASAFH QQFVQTLNSA SGSYAAAEAT IASQLQTAQH DLLGAVNAPT ETLLGRPLIG DGAPGTATSP NGGAGGLLYG NGGNGYSATA SGVGGAGGSA GLIGNGGAGG AGGPNAPGGA GGNGGWLLGN GGIGGPGGAS SIPGMSGGAG GTGGAAGLLG WGANGGAGGL GDGVGVDRGT GGAGGRGGLL YGGYGVSGPG GDGRTVPLEI IHVTEPTVHA NVNGGPTSTI LVDTGSAGLV VSPEDVGGIL GVLHMGLPTG LSISGYSGGL YYIFATYTTT VDFGNGIVTA PTAVNVVLLS IPTSPFAIST YFSALLADPT TTPFEAYFGA VGVDGVLGVG PNAVGPGPSI PTMALPGDLN QGVLIDAPAG ELVFGPNPLP APNVEVVGSP ITTLYVKIDG GTPIPVPSII DSGGVTGTIP SYVIGSGTLP ANTNIEVYTS PGGDRLYAFN TNDYRPTVIS SGLMNTGFLP FRFQPVYIDY SPSGIGTTVF DHPA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggatgtg gagaacatag gttccacact gcgcgcggcg aatgccgcgg ctgccgcctc gaccaccgcg cttgcggccg ctggcgctga tgaggtatcg gcggcggtgg cagcgctgtt tgccaggttc ggtcaggaat atcaagcggt cagcgcgcag gcgagcgctt tccatcaaca gttcgtgcag acgctgaact cggcgtcagg atcgtatgcg gccgcggagg ccaccatcgc gtcacagttg cagaccgcgc agcacgatct gctgggcgcg gtcaatgcac caaccgaaac gttgttgggg cgtccgctaa tcggcgacgg agcacccggg acggcaacga gtccgaatgg cggggcgggt gggctgctgt acggcaacgg cggcaacggt tattccgcga cggcgtcggg ggtcsgcggc ggggccggcg gttccgcggg gttgatcggc aatggcggcg ccgggggagc cggcggaccc aacgcccccg ggggagccgg cggcaacggt ggctggctgc tcggcaacgg cgggatcggc gggcccgggg gcgcgtcgag catccccggc atgagtggtg gagccggcgg aaccggcggt gccgcaggac ttttgggctg gggagcgaac ggcggagccg gcggcctcgg tgatggagtc ggtgtcgatc gtggcacggg cggcgccgga ggccgcggcg gcctgttgta tggcggatac ggcgtcagtg ggccaggcgg cgacggcaga accgtcccgc tggagataat tcatgtcaca gagccgacgg tacatgccaa cgtcaacggc ggaccgacgt caaccattct ggtcgacacc ggatccgctg gtcttgttgt ctcgcctgag gatgtcgggg gaatcctggg agtgcttcac atgggcctcc caaccggatt gagcatcagc ggttacagcg gggggctgta ctacatcttc gccacgtata ccacgacggt ggacttcggg aatggcatcg tcaccgcgcc gaccgccgtt aatgtcgtcc tcttgtccat cccaacgtcc cccttcgcca tttcgaccta cttcagcgcc ttgctggccg atccgacaac aactccgttc gaagcctatt tcggtgccgt cggcgtggac ggcgttctgg gagttgggcc caatgcggtg ggaccaggcc ccagcattcc gacgatggcg ttaccgggtg acctcaacca gggagtgctc atcgacgcac ccgcaggtga gctcgtgttc ggtcccaacc cgctacctgc gcccaacgtc gaggtcgtcg gatcgccgat caccaccctg tacgtaaaga tcgatggtgg gactcccata cccgtcccct cgatcatcga ttccggtggg gtaacgggaa ccatcccgtc atatgtcatc ggatccggaa ccctgccggc gaacacaaac attgaggtct acaccagccc cggcggtgat cggctctacg cgttcaacac aaacgattac cgcccgaccg tcatttcatc cggcctgatg aataccgggt tcttgccctt cagattccag ccggtgtaca tcgactacag ccccagcggt atagggacaa cagtctttga tcatccggcg taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa