MytuD.18213.o

PE FAMILY PROTEIN. POSSIBLE LIPASE.

CENTER ID: MytuD.18213.o
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18213.o.A1.GE34277 full length 1 310
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.1833
RefSeq: YP_177825
TubercuList: Rv1646
MTB Network Portal: Rv1646
UniProt: L7N681

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSFLTVAPDM VTAAAGNLES VGSALNEAAA AAAPATVGLA APAADRVSAV VAAMLGAYAR DFQGISAQIA GFHNQFVGAL RGGAAAYASA EAANVQQTVV NAVNAPAQAL LGHPLIGPET VGSSAAAVSF GFGPLLLAGS DPLLAVPFSY PASLPTPFGP VTMTLNGSFD PLTQQVVFDS GSLTAPAPFV YGLGAVGPAL TTMTALQNSG TAFSGAVQSG NLLGAAGALL QAPGNAVTGF LFGQTAISQS IPGPSNLGYE SVGISVPVGG LLAPLQPVTV TLTPTSGMPT AIQLSGTQFG GLLPALLNGF
NT Sequence
atgtcgtttc tcaccgtggc gccggacatg gtaacggcgg ccgccgggaa tttggaaagc gttggctcgg cactgaatga ggccgctgcg gcggcggcgc cagccacggt tgggctggcg gccccggccg cggatcgggt gtcggcggtc gtcgcggcga tgttgggggc atatgcccgg gattttcaag gcatcagtgc tcagatcgcg ggttttcata accagttcgt gggcgcgttg cggggcggtg cggccgccta cgccagcgcc gaagccgcca acgtccagca gaccgtggtg aacgccgtga atgcgcccgc ccaggcgctg ttggggcacc cgttgatcgg gcccgagacg gtcggctcca gcgccgccgc ggtctccttc ggcttcggcc cgttgctcct cgctggtagc gatccgctgc tggccgtgcc attcagctat ccggccagtc tgcccacccc attcggtcca gtaacgatga cgctcaacgg gtcgtttgat ccgcttaccc aacaggttgt tttcgactcg ggatcactca ccgcgcccgc tccgttcgtg tacggtcttg gtgcggtagg tccagctctc accaccatga ccgcgctgca aaacagcggc acagcatttt ccggcgcggt gcaaagcggg aacctgctag gggccgcggg cgcgcttctg caagctcccg gcaacgcggt gaccggcttc ctgtttggcc aaacagcgat atcgcagtcg ataccggggc catcgaatct gggctacgag tcggtgggta tcagcgttcc ggtcgggggg ctcttggctc cgctgcagcc cgtgacggtc acgttgacgc ccacatctgg tatgccgact gccattcaat tgagtggtac gcagtttggc ggccttcttc ccgccctact caacggtttc taa
Details for MytuD.18213.o.A1.GE34277
HARVESTED ON: 12/19/2011
SEQUENCED ON: 12/28/2011
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 82
PERCENT COVERAGE: 108
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSFLTVAPD MVTAAAGNLE SVGSALNEAA AAAAPATVGL AAPAADRVSA VVAAMLGAYA RDFQGISAQI AGFHNQFVGA LRGGAAAYAS AEAANVQQTV VNAVNAPAQA LLGHPLIGPE TVGSSAAAVS FGFGPLLLAG SDPLLAVPFS YPASLPTPFG PVTMTLNGSF DPLTQQVVFD SGSLTAPAPF VYGLGAVGPA LTTMTALQNS GTAFSGAVQS GNLLGAAGAL LQAPGNAVTG FLFGPNSDIA VDTGAIESGL XVGGYQRSXR GALGSAAARD GHVDAHIWXX DXXXXXXTXF GXXXPXYXXX XXTAXXXXXX FSXXXXXXKX XXXCXXXXXX
Validated NT Sequence
annnattttg tttnncttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgtttctc accgtggcgc cggacatggt aacggcggcc gccgggaatt tggaaagcgt tggctcggca ctgaatgagg ccgctgcggc ggcggcgcca gccacggttg ggctggcggc cccggccgcg gatcgggtgt cggcggtcgt cgcggcgatg ttgggggcat atgcccggga ttttcaaggc atcagtgctc agatcgcggg ttttcataac cagttcgtgg gcgcgttgcg gggcggtgcg gccgcctacg ccagcgccga agccgccaac gtccagcaga ccgtggtgaa cgccgtgaat gcgcccgccc aggcgctgtt ggggcacccg ttgatcgggc ccgagacggt cggctccagc gccgccgcgg tctccttcgg cttcggcccg ttgctcctcg ctggtagcga tccgctgctg gccgtgccat tcagctatcc ggccagtctg cccaccccat tcggtccagt aacgatgacg ctcaacgggt cgtttgatcc gcttacccaa caggttgttt tcgactcggg atcactcacc gcgcccgctc cgttcgtgta cggtcttggt gcggtaggtc cagctctcac caccatgacc gcgctgcaaa acagcggcac agcattttcc ggcgcggtgc aaagcgggaa cctgctaggg gccgcgggcg cgcttctgca agctcccggc aacgcggtga ccggcttcct gtttgggcca aacagcgata tcgcagtcga taccggggcc atcgaatctg ggctacnagt cggtgggtat cagcgttccn gtcggggggc tcttggntcc gctgcagccc gtgacggtca cgttgacgcc cacatctggn atgncgactg ncannnnntt gannngacgc antttggnnn nnncntcccg ncctactnan nnnnnnanna aacagcnnga annnnnngca nnnncttctc nnngnnnnnn nngcnnnnna aannnnctnn nnnntgcnnn nnnnnnnnnn nnnnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSFLTVAPD MVTAAAGNLE SVGSALNEAA AAAAPATVGL AAPAADRVSA VVAAMLGAYA RDFQGISAQI AGFHNQFVGA LRGGAAAYAS AEAANVQQTV VNAVNAPAQA LLGHPLIGPE TVGSSAAAVS FGFGPLLLAG SDPLLAVPFS YPASLPTPFG PVTMTLNGSF DPLTQQVVFD SGSLTAPAPF VYGLGAVGPA LTTMTALQNS GTAFSGAVQS GNLLGAAGAL LQAPGNAVTG FLFGQTAISQ SIPGPSNLGY ESVGISVPVG GLLAPLQPVT VTLTPTSGMP TAIQLSGTQF GGLLPALLNG F
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgtttctc accgtggcgc cggacatggt aacggcggcc gccgggaatt tggaaagcgt tggctcggca ctgaatgagg ccgctgcggc ggcggcgcca gccacggttg ggctggcggc cccggccgcg gatcgggtgt cggcggtcgt cgcggcgatg ttgggggcat atgcccggga ttttcaaggc atcagtgctc agatcgcggg ttttcataac cagttcgtgg gcgcgttgcg gggcggtgcg gccgcctacg ccagcgccga agccgccaac gtccagcaga ccgtggtgaa cgccgtgaat gcgcccgccc aggcgctgtt ggggcacccg ttgatcgggc ccgagacggt cggctccagc gccgccgcgg tctccttcgg cttcggcccg ttgctcctcg ctggtagcga tccgctgctg gccgtgccat tcagctatcc ggccagtctg cccaccccat tcggtccagt aacgatgacg ctcaacgggt cgtttgatcc gcttacccaa caggttgttt tcgactcggg atcactcacc gcgcccgctc cgttcgtgta cggtcttggt gcggtaggtc cagctctcac caccatgacc gcgctgcaaa acagcggcac agcattttcc ggcgcggtgc aaagcgggaa cctgctaggg gccgcgggcg cgcttctgca agctcccggc aacgcggtga ccggcttcct gtttggccaa acagcgatat cgcagtcgat accggggcca tcgaatctgg gctacgagtc ggtgggtatc agcgttccgg tcggggggct cttggctccg ctgcagcccg tgacggtcac gttgacgccc acatctggta tgccgactgc cattcaattg agtggtacgc agtttggcgg ccttcttccc gccctactca acggtttcta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a