SSGCID: Target Page

MytuD.18166.a

UPF0051 protein Rv1462/MT1509

CENTER ID:	MytuD.18166.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.18166.a.B1.GE37911	full length		1	397

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|83332.12.peg.1633
RefSeq:	NP_215978.1
RefSeq:	YP_006514845.1
TubercuList:	Rv1462
MTB Network Portal:	Rv1462
UniProt:	P9WFP5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTAPGLTAAV EGIAHNKGEL FASFDVDAFE VPHGRDEIWR FTPLRRLRGL HDGSARATGS ATITVSERPG VYTQTVRRGD PRLGEGGVPT DRVAAQAFSS FNSATLVTVE RDTQVVEPVG ITVTGPGEGA VAYGHLQVRI EELGEAVVVI DHRGGGTYAD NVEFVVDDAA RLTAVWIADW ADNTVHLSAH HARIGKDAVL RHVTVMLGGD VVRMSAGVRF CGAGGDAELL GLYFADDGQH LESRLLVDHA HPDCKSNVLY KGALQGDPAS SLPDAHTVWV GDVLIRAQAT GTDTFEVNRN LVLTDGARAD SVPNLEIETG EIVGAGHASA TGRFDDEQLF YLRSRGIPEA QARRLVVRGF FGEIIAKIAV PEVRERLTAA IEHELEITES TEKTTVS

NT Sequence

atgacggctc cgggactgac agcagccgtc gaggggatcg cacacaacaa gggcgagctg ttcgcctcct ttgacgtgga cgcgttcgag gttccgcacg gccgcgacga gatctggcgg ttcaccccgt tgcggcggct gcgtggcctg cacgacggct ccgcgcgggc caccggtagc gccacgatca cggtcagcga gcggccgggc gtatacaccc agaccgtgcg ccgcggcgat ccacgactgg gcgagggcgg cgtacccacc gaccgcgttg ccgcccaagc gttttcgtcg ttcaactccg cgactctggt caccgtcgag cgcgacaccc aggtcgtcga gccggtaggc atcaccgtga ccgggccggg ggagggcgcg gtggcctatg ggcacctgca ggtgcgtatc gaggagcttg gcgaggcggt cgtggtcatc gaccaccggg gcggcggaac ctacgccgac aacgtcgagt tcgttgtcga cgacgccgct cggctgaccg ccgtgtggat cgccgactgg gccgacgaca ccgttcacct cagcgcgcac catgctcgga tcggcaagga cgcggtgctg cgccacgtca ccgtcatgtt gggcggcgac gtggtgcgaa tgtcggcggg cgtgcggttc tgcggtgcgg gtggggacgc ggaactgctg gggctgtatt tcgccgacga cggccagcac ctggagtcgc ggctgctggt ggaccacgcc caccccgact gcaagtcgaa cgtgctgtat aagggtgcac tgcaaggtga tccggcgtcg tcgttgcccg acgcacacac ggtctgggtg ggtgacgtgc tgatccgtgc gcaggccacc ggcaccgaca ccttcgaggt gaaccggaac ctggtgctca ccgacggcgc gcgtgccgac tcggtgccca acctggagat cgagaccggc gagatcgtcg gcgccggaca cgccagcgcc accggtcgct tcgacgatga gcaattgttc tacctgcgtt cgcgcggtat tcccgaagca caggcccgcc ggctggtggt ccgcggcttc ttcggtgaga tcatcgccaa gatcgcggtg cccgaggtac gcgagcgcct gaccgcagcc atcgaacacg agctggaaat cacggaatca acggaaaaga caacagtctc atga

Details for MytuD.18166.a.B1.GE37911

HARVESTED ON:	4/10/2014
SEQUENCED ON:	4/8/2014
EXPECTED MW:	44kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	67

Validated AA Sequence

Validated NT Sequence

gannncnctn gaatantttt gtttaacttt aagaaggaga tataccatgg ctcaccacca ccaccaccat atgacggctc cgggactgac agcagccgtc gaggggatcg cacacaacaa gggcgagctg ttcgcctcct ttgacgtgga cgcgttcgag gttccgcacg gccgcgacga gatctggcgg ttcaccccgt tgcggcggct gcgtggcctg cacgacggct ccgcgcgggc caccggtagc gccacgatca cggtcagcga gcggccgggc gtatacaccc agaccgtgcg ccgcggcgat ccacgactgg gcgagggcgg cgtacccacc gaccgcgttg ccgcccaagc gttttcgtcg ttcaactccg cgactctggt caccgtcgag cgcgacaccc aggtcgtcga gccggtaggc atcaccgtga ccgggccggg ggagggcgcg gtggcctatg ggcacctgca ggtgcgtatc gaggagcttg gcgaggcggt cgtggtcatc gaccaccggg gcggcggaac ctacgccgac aacgtcgagt tcgttgtcga cgacgccgct cggctgaccg ccgtgtggat cgccgactgg gccgacaaca ccgttcacct cagcgcgcac catgctcgga tcggcaagga cgcggtgctg cgccacgtca ccgtcatgtt gggcggcgac gtggtgcgaa tgtcggcggg cgtgcggttc tgcggtgcgg gtggggacgc ggaactgctg gggctgtatt tcgccgacga cggccagcac ctggagtcgc ggctgctggt ggaccacgcc caccccgact gcaagtcgaa cgtgctgtat naagggtgca ctgcaaggtg atccggcgtc gtcgttgccc gacgcacaca cggtctgggt gggtgacgtg ctgatccgtg cgcagccacc ggcaccgaca ccttcgaggt gaaccngaac ctggtgctca ccgacgcgcg cgtgccgact cggtgcccaa cctggagatc gagaccnnga gaatcgtcgn cgccggacnc gccngcgcca ccggtcgctt cgacgatgag caattgtctn cgcnnnncnn nnnntncnna gcnnagncnn cgntgnnncn cgnntnntcg nnntcatcnc aannatnncn nnnnnnannn cncnaggnnn cngnnccgcg ccntcnnnnn ngagnntngn aaantntnna c

Expected Protein Sequence

MAHHHHHHMT APGLTAAVEG IAHNKGELFA SFDVDAFEVP HGRDEIWRFT PLRRLRGLHD GSARATGSAT ITVSERPGVY TQTVRRGDPR LGEGGVPTDR VAAQAFSSFN SATLVTVERD TQVVEPVGIT VTGPGEGAVA YGHLQVRIEE LGEAVVVIDH RGGGTYADNV EFVVDDAARL TAVWIADWAD NTVHLSAHHA RIGKDAVLRH VTVMLGGDVV RMSAGVRFCG AGGDAELLGL YFADDGQHLE SRLLVDHAHP DCKSNVLYKG ALQGDPASSL PDAHTVWVGD VLIRAQATGT DTFEVNRNLV LTDGARADSV PNLEIETGEI VGAGHASATG RFDDEQLFYL RSRGIPEAQA RRLVVRGFFG EIIAKIAVPE VRERLTAAIE HELEITESTE KTTVS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacgg ctccgggact gacagcagcc gtcgagggga tcgcacacaa caagggcgag ctgttcgcct cctttgacgt ggacgcgttc gaggttccgc acggccgcga cgagatctgg cggttcaccc cgttgcggcg gctgcgtggc ctgcacgacg gctccgcgcg ggccaccggt agcgccacga tcacggtcag cgagcggccg ggcgtataca cccagaccgt gcgccgcggc gatccacgac tgggcgaggg cggcgtaccc accgaccgcg ttgccgccca agcgttttcg tcgttcaact ccgcgactct ggtcaccgtc gagcgcgaca cccaggtcgt cgagccggta ggcatcaccg tgaccgggcc gggggagggc gcggtggcct atgggcacct gcaggtgcgt atcgaggagc ttggcgaggc ggtcgtggtc atcgaccacc ggggcggcgg aacctacgcc gacaacgtcg agttcgttgt cgacgacgcc gctcggctga ccgccgtgtg gatcgccgac tgggccgacg acaccgttca cctcagcgcg caccatgctc ggatcggcaa ggacgcggtg ctgcgccacg tcaccgtcat gttgggcggc gacgtggtgc gaatgtcggc gggcgtgcgg ttctgcggtg cgggtgggga cgcggaactg ctggggctgt atttcgccga cgacggccag cacctggagt cgcggctgct ggtggaccac gcccaccccg actgcaagtc gaacgtgctg tataagggtg cactgcaagg tgatccggcg tcgtcgttgc ccgacgcaca cacggtctgg gtgggtgacg tgctgatccg tgcgcaggcc accggcaccg acaccttcga ggtgaaccgg aacctggtgc tcaccgacgg cgcgcgtgcc gactcggtgc ccaacctgga gatcgagacc ggcgagatcg tcggcgccgg acacgccagc gccaccggtc gcttcgacga tgagcaattg ttctacctgc gttcgcgcgg tattcccgaa gcacaggccc gccggctggt ggtccgcggc ttcttcggtg agatcatcgc caagatcgcg gtgcccgagg tacgcgagcg cctgaccgca gccatcgaac acgagctgga aatcacggaa tcaacggaaa agacaacagt ctcatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat