MytuD.18062.a

DNA topoisomerase 1

CENTER ID: MytuD.18062.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18062.a.B1.GE37009 full length 1 934
MytuD.18062.a.B11.GE37604 full length 1 934
MytuD.18062.a.B2.GE37607 N-term domain 1 1 623
MytuD.18062.a.B3.GE37608 N-term domain 2 1 610
MytuD.18062.a.B21.GE37955 N-term domain 1 1 623
MytuD.18062.a.B31.GE37956 N-term domain 2 1 610
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18062.a.B11.PW37435 full length 1 934
MytuD.18062.a.B2.PW37563 N-term domain 1 1 623
MytuD.18062.a.B21.PW37505 N-term domain 1 1 623
MytuD.18062.a.B3.PW37565 N-term domain 2 1 610
MytuD.18062.a.B3.PW37432 N-term domain 2 1 610
MytuD.18062.a.B31.PW37506 N-term domain 2 1 610

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.4065
RefSeq: NP_218163.1
RefSeq: YP_006517133.1
TubercuList: Rv3646c
MTB Network Portal: Rv3646c
UniProt: P9WG49

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MADPKTKGRG SGGNGSGRRL VIVESPTKAR KLASYLGSGY IVESSRGHIR DLPRAASDVP AKYKSQPWAR LGVNVDADFE PLYIISPEKR STVSELRGLL KDVDELYLAT DGDREGEAIA WHLLETLKPR IPVKRMVFHE ITEPAIRAAA EHPRDLDIDL VDAQETRRIL DRLYGYEVSP VLWKKVAPKL SAGRVQSVAT RIIVARERDR MAFRSAAYWD ILAKLDASVS DPDAAPPTFS ARLTAVAGRR VATGRDFDSL GTLRKGDEVI VLDEGSATAL AAGLDGTQLT VASAEEKPYA RRPYPPFMTS TLQQEASRKL RFSAERTMSI AQRLYENGYI TYMRTDSTTL SESAINAART QARQLYGDEY VAPAPRQYTR KVKNAQEAHE AIRPAGETFA TPDAVRRELD GPNIDDFRLY ELIWQRTVAS QMADARGMTL SLRITGMSGH QEVVFSATGR TLTFPGFLKA YVETVDELVG GEADDAERRL PHLTPGQRLD IVELTPDGHA TNPPARYTEA SLVKALEELG IGRPSTYSSI IKTIQDRGYV HKKGSALVPS WVAFAVTGLL EQHFGRLVDY DFTAAMEDEL DEIAAGNERR TNWLNNFYFG GDHGVPDSVA RSGGLKKLVG INLEGIDARE VNSIKLFDDT HGRPIYVRVG KNGPYLERLV AGDTGEPTPQ RANLSDSITP DELTLQVAEE LFATPQQGRT LGLDPETGHE IVAREGRFGP YVTEILPEPA ADAAAAAQGV KKRQKAAGPK PRTGSLLRSM DLQTVTLEDA LRLLSLPRVV GVDPASGEEI TAQNGRYGPY LKRGNDSRSL VTEDQIFTIT LDEALKIYAE PKRRGRQSAS APPLRELGTD PASGKPMVIK DGRFGPYVTD GETNASLRKG DDVASITDER AAELLADRRA RGPAKRPARK AARKVPAKKA AKRD
NT Sequence
ttggctgacc cgaaaacgaa gggccgtggc agcggcggca atggcagcgg ccggcgactg gtcatcgtcg agtcgcccac caaggcgcgc aagctggcct cctacctggg ctctggctac atcgtcgagt cctcccgggg gcacatccgt gacttgccgc gggccgcgtc ggatgtaccc gcaaagtaca agtcgcagcc gtgggcgcgg ctcggggtca acgtcgacgc cgacttcgaa ccgctctaca tcatcagccc ggagaaacgg agcaccgtca gcgagctcag gggcctgctc aaagacgtgg acgagctgta tctggccacg gatggggacc gtgagggcga agctattgcc tggcatctgc tggaaaccct caaaccgcgc ataccggtaa agcggatggt cttccacgag atcaccgaac cggcgatccg cgccgccgcc gagcaccccc gcgacctaga catcgacctg gtcgacgcgc aggagacccg gcgcatcctg gaccggctgt acggctacga agtcagccca gtgctgtgga agaaggtcgc ccccaagttg tcggcgggcc gggtgcagtc ggtggccacc cgcatcatcg tggcgcgcga acgcgaccgc atggcgttcc gcagcgcggc ctactgggac atccttgcca agctggatgc cagcgtgtcc gacccggacg ccgcgccgcc caccttcagc gcccggctga cggccgtggc tggccggcgg gtggccactg gccgcgattt cgactcgctg ggcacgctgc gcaaaggcga cgaagtcatt gtgctcgacg aggggagcgc gaccgcgttg gccgcgggcc tggatggcac gcagctgacc gtggcctcgg ccgaggagaa gccctacgcc cggcgcccgt acccgccgtt catgacctcc acgctgcagc aagaggccag ccgcaagctg cggttctccg ccgagcggac gatgagcatc gcccagcggc tgtacgaaaa cggctacatc acctatatgc gtaccgactc caccacgctg tcggagtcgg cgatcaacgc cgcacgtacc caggcgcgcc agctctacgg cgacgagtac gtcgcgccgg cgccgcgcca atacacccgc aaggtgaaga acgcccagga agcgcacgag gctatccggc ccgccggtga aacgtttgcc accccggacg cggtgcgtcg cgaactcgac ggtcccaaca ttgatgattt ccggctctat gagctgattt ggcaacgcac cgtagcctcg cagatggccg atgcgcgggg catgacgctg agcctgcgga tcactggcat gtcggggcac caggaggtgg tgttctccgc gaccggacgc accttgacgt tcccgggctt cctcaaggcc tacgtggaga ccgtggacga gctggtcggc ggcgaggctg acgatgccga gcggcgactg ccccatctga ccccgggtca acggttggac atcgtcgagt tgaccccaga cggccatgcc accaacccgc cggcccgcta caccgaggcg tcgctggtca aagcgctcga ggagctgggc atcggccgcc cgtcgaccta ctcgtcgatc atcaagacca tccaggatcg cggctacgtg cacaagaagg gcagtgcact ggtgccgtca tgggtggcgt tcgcggtaac cggtctgctc gagcagcatt tcggtcggct cgtcgactac gacttcaccg cggcgatgga agacgagctc gacgagatcg ccgccggcaa cgagcgccgc accaactggc tcaacaactt ctactttggt ggcgatcacg gtgtgcccga ttcggtagcc cgatcgggtg gcctcaagaa gcttgtcggg atcaatctcg agggcatcga cgcacgagaa gtaaactcta tcaagctttt tgacgacacc cacggacgcc ccatatatgt tcgggtgggc aagaacggtc cctacctgga acgtttggtg gccggcgaca ccggtgagcc cacgccgcag cgggccaacc tcagcgactc gattaccccg gacgagctga ctctacaggt ggccgaagag ctctttgcca caccgcaaca gggacggact ttgggcttgg acccagaaac cggccacgag atcgtggcca gggaaggccg gtttgggccg tatgtgaccg agatcctgcc ggagcctgcg gctgatgcgg ccgcggccgc tcagggagtc aagaaacgcc agaaggccgc cgggcccaaa ccgcgcaccg gttcgttgct gcggagcatg gacctacaga cggtcaccct cgaagacgcg ctgaggctgc tgtcactgcc gcgcgtggtc ggagtggacc ccgcctcggg tgaggagatc accgcgcaga acgggcgcta cggaccgtat ctaaagcgcg gcaacgattc tcgatcactg gtcaccgaag accagatatt caccatcacg ctcgacgaag ccctgaagat ctacgcagag ccgaaacgtc gtggccggca aagcgcttcg gctccgccgc tgcgcgagct gggaacagat ccggcgtcgg gcaagccaat ggtcatcaag gacggccgat tcgggccgta cgtcaccgac ggtgagacca atgccagcct gcgtaagggc gacgacgtgg cttccataac cgacgagcgc gccgccgagc tgttggccga tcgccgagcc cggggtccgg caaaacggcc agccaggaaa gctgcccgga aggtgccggc gaagaaggca gccaagcgcg actag
Details for MytuD.18062.a.B1.GE37009
HARVESTED ON: 5/28/2013
SEQUENCED ON: 6/8/2013
EXPECTED MW: 103kDa
OBSERVED MW: 103kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 17
Validated AA Sequence
MDLQTVTLED ALRLLSLPRV VGVDPASGEE ITAQNGRYGP YLKRGNDSRS LVTEDQIFTI TLDEALKIYA EPKRRGRQSA SAPPLRELGT DPASGKPMVI KDGRFGPYVT DGETNASLRK GDDVASITDE RAAELLADRR ARGPAKRPAR KAARKVPAKK AAKRD
Validated NT Sequence
atcttactca cttagtcgcg cttggctgcc ttcttcgccg gcaccttccg ggcagctttc ctggctggcc gttttgccgg accccgggct cggcgatcgg ccaacagctc ggcggcgcgc tcgtcggtta tggaagccac gtcgtcgccc ttacgcaggc tggcattggt ctcaccgtcg gtgacgtacg gcccgaatcg gccgtccttg atgaccattg gcttgcccga cgccggatct gttcccagct cgcgcagcgg cggagccgaa gcgctttgcc ggccacgacg tttcggctct gcgtagatct tcagggcttc gtcgagcgtg atggtgaata tctggtcttc ggtgaccagt gatcgagaat cgttgccgcg ctttagatac ggtccgtagc gcccgttctg cgcggtgatc tcctcacccg aggcggggtc cactccgacc acgcgcggca gtgacagcag cctcagcgcg tcttcgaggg tgaccgtctg taggtccatg ctccgcagca acgaaccggt gcgcggtttg ggcccggcgg ccttctggcg tttcttgact ccctgagcgg ccgcggccgc atcagccgca ggctccggca ggatctcggt cacatacggc ccaaaccggc cttccctggc cacgatctcg tggccggttt ctgggtccaa gcccaaagtc cgtccctgtt gcggtgtggc aaagagctct tcggccacct gtagagtcag ctcgtccggg gtaatcgagt cgctgangtt ggcccgctgc ggcgtgggct caccggtgtc gccggccacc aaacgttcca ggtagggacc gttcttgccc acccgaacat atatggggcg tccgtgggtg tcgtcaaaaa gcttgataga ntttacttct cgtgcgtcga tgccctcgag antgatcccg acaagcttcn tgaggccncc cgatcgggct ac
Expected Protein Sequence
MAHHHHHHMA DPKTKGRGSG GNGSGRRLVI VESPTKARKL ASYLGSGYIV ESSRGHIRDL PRAASDVPAK YKSQPWARLG VNVDADFEPL YIISPEKRST VSELRGLLKD VDELYLATDG DREGEAIAWH LLETLKPRIP VKRMVFHEIT EPAIRAAAEH PRDLDIDLVD AQETRRILDR LYGYEVSPVL WKKVAPKLSA GRVQSVATRI IVARERDRMA FRSAAYWDIL AKLDASVSDP DAAPPTFSAR LTAVAGRRVA TGRDFDSLGT LRKGDEVIVL DEGSATALAA GLDGTQLTVA SAEEKPYARR PYPPFMTSTL QQEASRKLRF SAERTMSIAQ RLYENGYITY MRTDSTTLSE SAINAARTQA RQLYGDEYVA PAPRQYTRKV KNAQEAHEAI RPAGETFATP DAVRRELDGP NIDDFRLYEL IWQRTVASQM ADARGMTLSL RITGMSGHQE VVFSATGRTL TFPGFLKAYV ETVDELVGGE ADDAERRLPH LTPGQRLDIV ELTPDGHATN PPARYTEASL VKALEELGIG RPSTYSSIIK TIQDRGYVHK KGSALVPSWV AFAVTGLLEQ HFGRLVDYDF TAAMEDELDE IAAGNERRTN WLNNFYFGGD HGVPDSVARS GGLKKLVGIN LEGIDAREVN SIKLFDDTHG RPIYVRVGKN GPYLERLVAG DTGEPTPQRA NLSDSITPDE LTLQVAEELF ATPQQGRTLG LDPETGHEIV AREGRFGPYV TEILPEPAAD AAAAAQGVKK RQKAAGPKPR TGSLLRSMDL QTVTLEDALR LLSLPRVVGV DPASGEEITA QNGRYGPYLK RGNDSRSLVT EDQIFTITLD EALKIYAEPK RRGRQSASAP PLRELGTDPA SGKPMVIKDG RFGPYVTDGE TNASLRKGDD VASITDERAA ELLADRRARG PAKRPARKAA RKVPAKKAAK RD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttggctg acccgaaaac gaagggccgt ggcagcggcg gcaatggcag cggccggcga ctggtcatcg tcgagtcgcc caccaaggcg cgcaagctgg cctcctacct gggctctggc tacatcgtcg agtcctcccg ggggcacatc cgtgacttgc cgcgggccgc gtcggatgta cccgcaaagt acaagtcgca gccgtgggcg cggctcgggg tcaacgtcga cgccgacttc gaaccgctct acatcatcag cccggagaaa cggagcaccg tcagcgagct caggggcctg ctcaaagacg tggacgagct gtatctggcc acggatgggg accgtgaggg cgaagctatt gcctggcatc tgctggaaac cctcaaaccg cgcataccgg taaagcggat ggtcttccac gagatcaccg aaccggcgat ccgcgccgcc gccgagcacc cccgcgacct agacatcgac ctggtcgacg cgcaggagac ccggcgcatc ctggaccggc tgtacggcta cgaagtcagc ccagtgctgt ggaagaaggt cgcccccaag ttgtcggcgg gccgggtgca gtcggtggcc acccgcatca tcgtggcgcg cgaacgcgac cgcatggcgt tccgcagcgc ggcctactgg gacatccttg ccaagctgga tgccagcgtg tccgacccgg acgccgcgcc gcccaccttc agcgcccggc tgacggccgt ggctggccgg cgggtggcca ctggccgcga tttcgactcg ctgggcacgc tgcgcaaagg cgacgaagtc attgtgctcg acgaggggag cgcgaccgcg ttggccgcgg gcctggatgg cacgcagctg accgtggcct cggccgagga gaagccctac gcccggcgcc cgtacccgcc gttcatgacc tccacgctgc agcaagaggc cagccgcaag ctgcggttct ccgccgagcg gacgatgagc atcgcccagc ggctgtacga aaacggctac atcacctata tgcgtaccga ctccaccacg ctgtcggagt cggcgatcaa cgccgcacgt acccaggcgc gccagctcta cggcgacgag tacgtcgcgc cggcgccgcg ccaatacacc cgcaaggtga agaacgccca ggaagcgcac gaggctatcc ggcccgccgg tgaaacgttt gccaccccgg acgcggtgcg tcgcgaactc gacggtccca acattgatga tttccggctc tatgagctga tttggcaacg caccgtagcc tcgcagatgg ccgatgcgcg gggcatgacg ctgagcctgc ggatcactgg catgtcgggg caccaggagg tggtgttctc cgcgaccgga cgcaccttga cgttcccggg cttcctcaag gcctacgtgg agaccgtgga cgagctggtc ggcggcgagg ctgacgatgc cgagcggcga ctgccccatc tgaccccggg tcaacggttg gacatcgtcg agttgacccc agacggccat gccaccaacc cgccggcccg ctacaccgag gcgtcgctgg tcaaagcgct cgaggagctg ggcatcggcc gcccgtcgac ctactcgtcg atcatcaaga ccatccagga tcgcggctac gtgcacaaga agggcagtgc actggtgccg tcatgggtgg cgttcgcggt aaccggtctg ctcgagcagc atttcggtcg gctcgtcgac tacgacttca ccgcggcgat ggaagacgag ctcgacgaga tcgccgccgg caacgagcgc cgcaccaact ggctcaacaa cttctacttt ggtggcgatc acggtgtgcc cgattcggta gcccgatcgg gtggcctcaa gaagcttgtc gggatcaatc tcgagggcat cgacgcacga gaagtaaact ctatcaagct ttttgacgac acccacggac gccccatata tgttcgggtg ggcaagaacg gtccctacct ggaacgtttg gtggccggcg acaccggtga gcccacgccg cagcgggcca acctcagcga ctcgattacc ccggacgagc tgactctaca ggtggccgaa gagctctttg ccacaccgca acagggacgg actttgggct tggacccaga aaccggccac gagatcgtgg ccagggaagg ccggtttggg ccgtatgtga ccgagatcct gccggagcct gcggctgatg cggccgcggc cgctcaggga gtcaagaaac gccagaaggc cgccgggccc aaaccgcgca ccggttcgtt gctgcggagc atggacctac agacggtcac cctcgaagac gcgctgaggc tgctgtcact gccgcgcgtg gtcggagtgg accccgcctc gggtgaggag atcaccgcgc agaacgggcg ctacggaccg tatctaaagc gcggcaacga ttctcgatca ctggtcaccg aagaccagat attcaccatc acgctcgacg aagccctgaa gatctacgca gagccgaaac gtcgtggccg gcaaagcgct tcggctccgc cgctgcgcga gctgggaaca gatccggcgt cgggcaagcc aatggtcatc aaggacggcc gattcgggcc gtacgtcacc gacggtgaga ccaatgccag cctgcgtaag ggcgacgacg tggcttccat aaccgacgag cgcgccgccg agctgttggc cgatcgccga gcccggggtc cggcaaaacg gccagccagg aaagctgccc ggaaggtgcc ggcgaagaag gcagccaagc gcgactgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for MytuD.18062.a.B11.GE37604
HARVESTED ON: 12/16/2013
SEQUENCED ON: 12/20/2013
EXPECTED MW: 103kDa
OBSERVED MW: 103kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 85
PERCENT COVERAGE: 42
Validated AA Sequence
MAHHHHHHML ADPKTKGRGS GGNGSGRRLV IVESPTKARK LASYLGSGYI VESSRGHIRD LPRAASDVPA KYKSQPWARL GVNVDADFEP LYIISPEKRS TVSELRGLLK DVDELYLATD GDREGEAIAW HLLETLKPRI PVKRMVFHEI TEPAIRAAAE HPRDLDIDLV DAQETRRILD RLYGYEVSPV LWKKVAPKLS AGRVQSVATR IIVARERDRM AFRSAAYWDI LAKLDASVSD PDAAPPTFSA RLTAVAGRRV ATGRDFDSLG TLRKGDEVIV LDEGSATALA AGLDGTQLTV ASAEEKPYAR RPYPPFMTST LQQEAAQAAV XRRADDEHRP XAVRKXLXXL YGVRLHPPXX XXXDQXXXXX XXXXXXXXXX XXXXXXXHXX XXXXXXXXX
Validated NT Sequence
aggnncncta naannntttt gtttncttta agaaggagat ataccatggc tcaccaccac caccaccata tgttggctga cccgaaaacg aagggccgtg gcagcggcgg caatggcagc ggccggcgac tggtcatcgt cgagtcgccc accaaggcgc gcaagctggc ctcctacctg ggctctggct acatcgtcga gtcctcccgg gggcacatcc gtgacttgcc gcgggccgcg tcggatgtac ccgcaaagta caagtcgcag ccgtgggcgc ggctcggggt caacgtcgac gccgacttcg aaccgctcta catcatcagc ccggagaaac ggagcaccgt cagcgagctc aggggcctgc tcaaagacgt ggacgagctg tatctggcca cggatgggga ccgtgagggc gaagctattg cctggcatct gctggaaacc ctcaaaccgc gcataccggt aaagcggatg gtcttccacg agatcaccga accggcgatc cgcgccgccg ccgagcaccc ccgcgaccta gacatcgacc tggtcgacgc gcaggagacc cggcgcatcc tggaccggct gtacggctac gaagtcagcc cagtgctgtg gaagaaggtc gcccccaagt tgtcggcggg ccgggtgcag tcggtggcca cccgcatcat cgtggcgcgc gaacgcgacc gcatggcgtt ccgcagcgcg gcctactggg acatccttgc caagctggat gccagcgtgt ccgacccgga cgccgcgccg cccaccttca gcgcccggct gacggccgtg gctggccggc gggtggccac tggccgcgat ttcgactcgc tgggcacgct gcgcaaaggc gacgaagtca ttgtgctcga cgaggggagc gcgaccgcgt tggccgcggg cctggatggc acgcagctga ccgtggcctc ggccgaggag aagccctacg cccggcgccc gtacccgccg ttcatgacct ccacgctgca gcaagaggca gcgcaagctg cggtnntccg ccgagcggac gatgagcatc gcccancggc tgtacgaaaa cngctanntc anctttatgg cgtacgactc cacccnccnn tgntcgnnnn ngncgatcaa ngccnnnacg ntnccnnggn nnnnnnnnnt nnngnnnnnn natanntnnn gncnnnnnnn nnnntncacn nnnngnnana aannnnnnna anncnnnang ntt
Expected Protein Sequence
MAHHHHHHLA DPKTKGRGSG GNGSGRRLVI VESPTKARKL ASYLGSGYIV ESSRGHIRDL PRAASDVPAK YKSQPWARLG VNVDADFEPL YIISPEKRST VSELRGLLKD VDELYLATDG DREGEAIAWH LLETLKPRIP VKRMVFHEIT EPAIRAAAEH PRDLDIDLVD AQETRRILDR LYGYEVSPVL WKKVAPKLSA GRVQSVATRI IVARERDRMA FRSAAYWDIL AKLDASVSDP DAAPPTFSAR LTAVAGRRVA TGRDFDSLGT LRKGDEVIVL DEGSATALAA GLDGTQLTVA SAEEKPYARR PYPPFMTSTL QQEASRKLRF SAERTMSIAQ RLYENGYITY MRTDSTTLSE SAINAARTQA RQLYGDEYVA PAPRQYTRKV KNAQEAHEAI RPAGETFATP DAVRRELDGP NIDDFRLYEL IWQRTVASQM ADARGMTLSL RITGMSGHQE VVFSATGRTL TFPGFLKAYV ETVDELVGGE ADDAERRLPH LTPGQRLDIV ELTPDGHATN PPARYTEASL VKALEELGIG RPSTYSSIIK TIQDRGYVHK KGSALVPSWV AFAVTGLLEQ HFGRLVDYDF TAAMEDELDE IAAGNERRTN WLNNFYFGGD HGVPDSVARS GGLKKLVGIN LEGIDAREVN SIKLFDDTHG RPIYVRVGKN GPYLERLVAG DTGEPTPQRA NLSDSITPDE LTLQVAEELF ATPQQGRTLG LDPETGHEIV AREGRFGPYV TEILPEPAAD AAAAAQGVKK RQKAAGPKPR TGSLLRSMDL QTVTLEDALR LLSLPRVVGV DPASGEEITA QNGRYGPYLK RGNDSRSLVT EDQIFTITLD EALKIYAEPK RRGRQSASAP PLRELGTDPA SGKPMVIKDG RFGPYVTDGE TNASLRKGDD VASITDERAA ELLADRRARG PAKRPARKAA RKVPAKKAAK RD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttggctg acccgaaaac gaagggccgt ggcagcggcg gcaatggcag cggccggcga ctggtcatcg tcgagtcgcc caccaaggcg cgcaagctgg cctcctacct gggctctggc tacatcgtcg agtcctcccg ggggcacatc cgtgacttgc cgcgggccgc gtcggatgta cccgcaaagt acaagtcgca gccgtgggcg cggctcgggg tcaacgtcga cgccgacttc gaaccgctct acatcatcag cccggagaaa cggagcaccg tcagcgagct caggggcctg ctcaaagacg tggacgagct gtatctggcc acggatgggg accgtgaggg cgaagctatt gcctggcatc tgctggaaac cctcaaaccg cgcataccgg taaagcggat ggtcttccac gagatcaccg aaccggcgat ccgcgccgcc gccgagcacc cccgcgacct agacatcgac ctggtcgacg cgcaggagac ccggcgcatc ctggaccggc tgtacggcta cgaagtcagc ccagtgctgt ggaagaaggt cgcccccaag ttgtcggcgg gccgggtgca gtcggtggcc acccgcatca tcgtggcgcg cgaacgcgac cgcatggcgt tccgcagcgc ggcctactgg gacatccttg ccaagctgga tgccagcgtg tccgacccgg acgccgcgcc gcccaccttc agcgcccggc tgacggccgt ggctggccgg cgggtggcca ctggccgcga tttcgactcg ctgggcacgc tgcgcaaagg cgacgaagtc attgtgctcg acgaggggag cgcgaccgcg ttggccgcgg gcctggatgg cacgcagctg accgtggcct cggccgagga gaagccctac gcccggcgcc cgtacccgcc gttcatgacc tccacgctgc agcaagaggc cagccgcaag ctgcggttct ccgccgagcg gacgatgagc atcgcccagc ggctgtacga aaacggctac atcaccttta tgcgtaccga ctccaccacg ctgtcggagt cggcgatcaa cgccgcacgt acccaggcgc gccagctcta cggcgacgag tacgtcgcgc cggcgccgcg ccaatacacc cgcaaggtga agaacgccca ggaagcgcac gaggctatcc ggcccgccgg tgaaacgttt gccaccccgg acgcggtgcg tcgcgaactc gacggtccca acattgatga tttccggctc tatgagctga tttggcaacg caccgtagcc tcgcagatgg ccgatgcgcg gggcatgacg ctgagcctgc ggatcactgg catgtcgggg caccaggagg tggtgttctc cgcgaccgga cgcaccttga cgttcccggg cttcctcaag gcctacgtgg agaccgtgga cgagctggtc ggcggcgagg ctgacgatgc cgagcggcga ctgccccatc tgaccccggg tcaacggttg gacatcgtcg agttgacccc agacggccat gccaccaacc cgccggcccg ctacaccgag gcgtcgctgg tcaaagcgct cgaggagctg ggcatcggcc gcccgtcgac ctactcgtcg atcatcaaga ccatccagga tcgcggctac gtgcacaaga agggcagtgc actggtgccg tcatgggtgg cgttcgcggt aaccggtctg ctcgagcagc atttcggtcg gctcgtcgac tacgacttca ccgcggcgat ggaagacgag ctcgacgaga tcgccgccgg caacgagcgc cgcaccaact ggctcaacaa cttctacttt ggtggcgatc acggtgtgcc cgattcggta gcccgatcgg gtggcctcaa gaagcttgtc gggatcaatc tcgagggcat cgacgcacga gaagtaaact ctatcaagct ttttgacgac acccacggac gccccatata tgttcgggtg ggcaagaacg gtccctacct ggaacgtttg gtggccggcg acaccggtga gcccacgccg cagcgggcca acctcagcga ctcgattacc ccggacgagc tgactctaca ggtggccgaa gagctctttg ccacaccgca acagggacgg actttgggct tggacccaga aaccggccac gagatcgtgg ccagggaagg ccggtttggg ccgtatgtga ccgagatcct gccggagcct gcggctgatg cggccgcggc cgctcaggga gtcaagaaac gccagaaggc cgccgggccc aaaccgcgca ccggttcgtt gctgcggagc atggacctac agacggtcac cctcgaagac gcgctgaggc tgctgtcact gccgcgcgtg gtcggagtgg accccgcctc gggtgaggag atcaccgcgc agaacgggcg ctacggaccg tatctaaagc gcggcaacga ttctcgatca ctggtcaccg aagaccagat attcaccatc acgctcgacg aagccctgaa gatctacgca gagccgaaac gtcgtggccg gcaaagcgct tcggctccgc cgctgcgcga gctgggaaca gatccggcgt cgggcaagcc aatggtcatc aaggacggcc gattcgggcc gtacgtcacc gacggtgaga ccaatgccag cctgcgtaag ggcgacgacg tggcttccat aaccgacgag cgcgccgccg agctgttggc cgatcgccga gcccggggtc cggcaaaacg gccagccagg aaagctgccc ggaaggtgcc ggcgaagaag gcagccaagc gcgactgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for MytuD.18062.a.B2.GE37607
HARVESTED ON: 12/17/2013
SEQUENCED ON: 12/20/2013
EXPECTED MW: 70kDa
OBSERVED MW: 70kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 50
Validated AA Sequence
MTXTLQQEXS RKLRFSAERT MSIAQRLYEN GYITYMRTDS TTLSESAINA ARTQARQLYG DEYVAPAPRQ YTRKVKNAQE AHEAIRPAGE TFATPDAVRR ELDGPNIDDF RLYELIWQRT VASQMADARG MTLSLRITGM SGHQEVVFSA TGRTLTFPGF LKAYVETVDE LVGGEADDAE RRLPHLTPGQ RLDIVELTPD GHATNPPARY TEASLVKALE ELGIGRPSTY SSIIKTIQDR GYVHKKGSAL VPSWVAFAVT GLLEQHFGRL VDYDFTAAME DELDEIAAGN ERRTNWLNNF YFGGDHGVPD SVARSG
Validated NT Sequence
atcttactca cttaacccga tcgggctacc gaatcgggca caccgtgatc gccaccaaag tagaagttgt tgagccagtt ggtgcggcgc tcgttgccgg cggcgatctc gtcgagctcg tcttccatcg ccgcggtgaa gtcgtagtcg acgagccgac cgaaatgctg ctcgagcaga ccggttaccg cgaacgccac ccatgacggc accagtgcac tgcccttctt gtgcacgtag ccgcgatcct ggatggtctt gatgatcgac gagtaggtcg acgggcggcc gatgcccagc tcctcgagcg ctttgaccag cgacgcctcg gtgtagcggg ccggcgggtt ggtggcatgg ccgtctgggg tcaactcgac gatgtccaac cgttgacccg gggtcagatg gggcagtcgc cgctcggcat cgtcagcctc gccgccgacc agctcgtcca cggtctccac gtaggccttg aggaagcccg ggaacgtcaa ggtgcgtccg gtcgcggaga acaccacctc ctggtgcccc gacatgccag tgatccgcag gctcagcgtc atgccccgcg catcggccat ctgcgaggct acggtgcgtt gccaaatcag ctcatagagc cggaaatcat caatgttggg accgtcgagt tcgcgacgca ccgcgtccgg ggtggcaaac gtttcaccgg cgggccggat agcctcgtgc gcttcctggg cgttcttcac cttgcgggtg tattggcgcg gcgccggcgc gacgtactcg tcgccgtaga gctggcgcgc ctgggtacgt gcggcgttga tcgccgactc cgacagcgtg gtggagtcgg tacgcatata ggtgatgtag ccgttttcgt acagccgctg ggcgatgctc atcgtccgct cggcggagaa ccgcagcttg cggctggnct cttgctgcag cgtggnngtc atgaacggcg ggtanngncn nnnggcgtnn gnntnntcnn nggccgaggc cacnntcagc tgc
Expected Protein Sequence
MAHHHHHHMA DPKTKGRGSG GNGSGRRLVI VESPTKARKL ASYLGSGYIV ESSRGHIRDL PRAASDVPAK YKSQPWARLG VNVDADFEPL YIISPEKRST VSELRGLLKD VDELYLATDG DREGEAIAWH LLETLKPRIP VKRMVFHEIT EPAIRAAAEH PRDLDIDLVD AQETRRILDR LYGYEVSPVL WKKVAPKLSA GRVQSVATRI IVARERDRMA FRSAAYWDIL AKLDASVSDP DAAPPTFSAR LTAVAGRRVA TGRDFDSLGT LRKGDEVIVL DEGSATALAA GLDGTQLTVA SAEEKPYARR PYPPFMTSTL QQEASRKLRF SAERTMSIAQ RLYENGYITY MRTDSTTLSE SAINAARTQA RQLYGDEYVA PAPRQYTRKV KNAQEAHEAI RPAGETFATP DAVRRELDGP NIDDFRLYEL IWQRTVASQM ADARGMTLSL RITGMSGHQE VVFSATGRTL TFPGFLKAYV ETVDELVGGE ADDAERRLPH LTPGQRLDIV ELTPDGHATN PPARYTEASL VKALEELGIG RPSTYSSIIK TIQDRGYVHK KGSALVPSWV AFAVTGLLEQ HFGRLVDYDF TAAMEDELDE IAAGNERRTN WLNNFYFGGD HGVPDSVARS G
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttggctg acccgaaaac gaagggccgt ggcagcggcg gcaatggcag cggccggcga ctggtcatcg tcgagtcgcc caccaaggcg cgcaagctgg cctcctacct gggctctggc tacatcgtcg agtcctcccg ggggcacatc cgtgacttgc cgcgggccgc gtcggatgta cccgcaaagt acaagtcgca gccgtgggcg cggctcgggg tcaacgtcga cgccgacttc gaaccgctct acatcatcag cccggagaaa cggagcaccg tcagcgagct caggggcctg ctcaaagacg tggacgagct gtatctggcc acggatgggg accgtgaggg cgaagctatt gcctggcatc tgctggaaac cctcaaaccg cgcataccgg taaagcggat ggtcttccac gagatcaccg aaccggcgat ccgcgccgcc gccgagcacc cccgcgacct agacatcgac ctggtcgacg cgcaggagac ccggcgcatc ctggaccggc tgtacggcta cgaagtcagc ccagtgctgt ggaagaaggt cgcccccaag ttgtcggcgg gccgggtgca gtcggtggcc acccgcatca tcgtggcgcg cgaacgcgac cgcatggcgt tccgcagcgc ggcctactgg gacatccttg ccaagctgga tgccagcgtg tccgacccgg acgccgcgcc gcccaccttc agcgcccggc tgacggccgt ggctggccgg cgggtggcca ctggccgcga tttcgactcg ctgggcacgc tgcgcaaagg cgacgaagtc attgtgctcg acgaggggag cgcgaccgcg ttggccgcgg gcctggatgg cacgcagctg accgtggcct cggccgagga gaagccctac gcccggcgcc cgtacccgcc gttcatgacc tccacgctgc agcaagaggc cagccgcaag ctgcggttct ccgccgagcg gacgatgagc atcgcccagc ggctgtacga aaacggctac atcacctata tgcgtaccga ctccaccacg ctgtcggagt cggcgatcaa cgccgcacgt acccaggcgc gccagctcta cggcgacgag tacgtcgcgc cggcgccgcg ccaatacacc cgcaaggtga agaacgccca ggaagcgcac gaggctatcc ggcccgccgg tgaaacgttt gccaccccgg acgcggtgcg tcgcgaactc gacggtccca acattgatga tttccggctc tatgagctga tttggcaacg caccgtagcc tcgcagatgg ccgatgcgcg gggcatgacg ctgagcctgc ggatcactgg catgtcgggg caccaggagg tggtgttctc cgcgaccgga cgcaccttga cgttcccggg cttcctcaag gcctacgtgg agaccgtgga cgagctggtc ggcggcgagg ctgacgatgc cgagcggcga ctgccccatc tgaccccggg tcaacggttg gacatcgtcg agttgacccc agacggccat gccaccaacc cgccggcccg ctacaccgag gcgtcgctgg tcaaagcgct cgaggagctg ggcatcggcc gcccgtcgac ctactcgtcg atcatcaaga ccatccagga tcgcggctac gtgcacaaga agggcagtgc actggtgccg tcatgggtgg cgttcgcggt aaccggtctg ctcgagcagc atttcggtcg gctcgtcgac tacgacttca ccgcggcgat ggaagacgag ctcgacgaga tcgccgccgg caacgagcgc cgcaccaact ggctcaacaa cttctacttt ggtggcgatc acggtgtgcc cgattcggta gcccgatcgg gttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for MytuD.18062.a.B3.GE37608
HARVESTED ON: 12/17/2013
SEQUENCED ON: 12/20/2013
EXPECTED MW: 69kDa
OBSERVED MW: 69kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 76
Validated AA Sequence
MVFHEITEPA IRAAAEHPRD LDIDLVDAQE TRRILDRLYG YEVSPVLWKK VAPKLSAGRV QSVATRIIVA RERDRMAFRS AAYWDILAKL DASVSXPDAA PPTFSARLTA VAGRRVATGR DFDSLGTLRK GDEVIVLDEG SATALAAGLD GTQLTVASAE EKPYARRXYP PFMTSTLQQE ASRKLRFSAE RTMSIAQRLY ENGYITYMRT DSTTLSESAI NAARTQARQL YGDEYVAPAP RQYTRKVKNA QEAHEAIRPA GETFATPDAV RRELDGPNID DFRLYELIWQ RTVASQMADA RGMTLSLRIT GMSGHQEVVF SATGRTLTFP GFLKAYVETV DELVGGEADD AERRLPHLTP GQRLDIVELT PDGHATNPPA RYTEASLVKA LEELGIGRPS TYSSIIKTIQ DRGYVHKKGS ALVPSWVAFA VTGLLEQHFG RLVDYDFTAA MEDELDEIAA GNERRTNWLN NFYFG
Validated NT Sequence
atcttactca cttaaccaaa gtagaagttg ttgagccagt tggtgcggcg ctcgttgccg gcggcgatct cgtcgagctc gtcttccatc gccgcggtga agtcgtagtc gacgagccga ccgaaatgct gctcgagcag accggttacc gcgaacgcca cccatgacgg caccagtgca ctgcccttct tgtgcacgta gccgcgatcc tggatggtct tgatgatcga cgagtaggtc gacgggcggc cgatgcccag ctcctcgagc gctttgacca gcgacgcctc ggtgtagcgg gccggcgggt tggtggcatg gccgtctggg gtcaactcga cgatgtccaa ccgttgaccc ggggtcagat ggggcagtcg ccgctcggca tcgtcagcct cgccgccgac cagctcgtcc acggtctcca cgtaggcctt gaggaagccc gggaacgtca aggtgcgtcc ggtcgcggag aacaccacct cctggtgccc cgacatgcca gtgatccgca ggctcagcgt catgccccgc gcatcggcca tctgcgaggc tacggtgcgt tgccaaatca gctcatagag ccggaaatca tcaatgttgg gaccgtcgag ttcgcgacgc accgcgtccg gggtggcaaa cgtttcaccg gcgggccgga tagcctcgtg cgcttcctgg gcgttcttca ccttgcgggt gtattggcgc ggcgccggcg cgacgtactc gtcgccgtag agctggcgcg cctgggtacg tgcggcgttg atcgccgact ccgacagcgt ggtggagtcg gtacgcatat aggtgatgta gccgttttcg tacagccgct gggcgatgct catcgtccgc tcggcggaga accgcagctt gcggctggcc tcttgctgca gcgtggaggt catgaacggc gggtacnngc gccgggcgta gggcttctcc tcggccgagg ccacggtcag ctgcgtgcca tccaggcccg cggccaacgc ggtcgcgctc ccctcgtcga gcacaatgac ttcgtcgcct ttgcgcagcg tgcccagcga gtcgaaatcg cggccagtgg ccacccgccg nccagccacg gccgtcagcc gggcgctgaa ggtgggcggc gcggcgtccg gntcggacac gctggcatcc agcttggcaa ggatgtccca gtaggccgcg ctgcggaacg ccatgcggtc gcgttcgcgc gccacgatga tgcgggtggc caccgactgc acccggcccg ccgacaactt gggggcgacc ttcttccaca gcactgggct gacttcgtag ccgtacagcc ggtccaggat gcgccgggtc tcctgcgcgt cgaccaggtc gatgtctagg tcgcgggggt gctcggcggc ggcgcggatc gccggttcgg tgatctcgtg gaagaccatc cgctttaccg gtatgcgcgg tttgagggtt tccagcagat gccaggcaat agcttcgccc tcacggtccc catccgtggc cagatacagc tcgtccacgt ctttgagcag gcccctgagc tcgctgacgg tgctccgttt ctccgggctg atgatgtaga gcggttcgaa gtcggcgtcg acgttgaccc cgagccgcgc ccacggctgc gacttgtact ttgcgggtac atccgacgcg gcccgcggca agtcacggat gtgcccccgg gaggactcga cgatgtagcc agagcccagg taggaggcca gcttgcgcgc cttggtgggc gactcgacga tgaccagtcg ccggccgctg ccattgccgc cgctgccacg gcccttcgtt ttcgggtcag cc
Expected Protein Sequence
MAHHHHHHMA DPKTKGRGSG GNGSGRRLVI VESPTKARKL ASYLGSGYIV ESSRGHIRDL PRAASDVPAK YKSQPWARLG VNVDADFEPL YIISPEKRST VSELRGLLKD VDELYLATDG DREGEAIAWH LLETLKPRIP VKRMVFHEIT EPAIRAAAEH PRDLDIDLVD AQETRRILDR LYGYEVSPVL WKKVAPKLSA GRVQSVATRI IVARERDRMA FRSAAYWDIL AKLDASVSDP DAAPPTFSAR LTAVAGRRVA TGRDFDSLGT LRKGDEVIVL DEGSATALAA GLDGTQLTVA SAEEKPYARR PYPPFMTSTL QQEASRKLRF SAERTMSIAQ RLYENGYITY MRTDSTTLSE SAINAARTQA RQLYGDEYVA PAPRQYTRKV KNAQEAHEAI RPAGETFATP DAVRRELDGP NIDDFRLYEL IWQRTVASQM ADARGMTLSL RITGMSGHQE VVFSATGRTL TFPGFLKAYV ETVDELVGGE ADDAERRLPH LTPGQRLDIV ELTPDGHATN PPARYTEASL VKALEELGIG RPSTYSSIIK TIQDRGYVHK KGSALVPSWV AFAVTGLLEQ HFGRLVDYDF TAAMEDELDE IAAGNERRTN WLNNFYFG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttggctg acccgaaaac gaagggccgt ggcagcggcg gcaatggcag cggccggcga ctggtcatcg tcgagtcgcc caccaaggcg cgcaagctgg cctcctacct gggctctggc tacatcgtcg agtcctcccg ggggcacatc cgtgacttgc cgcgggccgc gtcggatgta cccgcaaagt acaagtcgca gccgtgggcg cggctcgggg tcaacgtcga cgccgacttc gaaccgctct acatcatcag cccggagaaa cggagcaccg tcagcgagct caggggcctg ctcaaagacg tggacgagct gtatctggcc acggatgggg accgtgaggg cgaagctatt gcctggcatc tgctggaaac cctcaaaccg cgcataccgg taaagcggat ggtcttccac gagatcaccg aaccggcgat ccgcgccgcc gccgagcacc cccgcgacct agacatcgac ctggtcgacg cgcaggagac ccggcgcatc ctggaccggc tgtacggcta cgaagtcagc ccagtgctgt ggaagaaggt cgcccccaag ttgtcggcgg gccgggtgca gtcggtggcc acccgcatca tcgtggcgcg cgaacgcgac cgcatggcgt tccgcagcgc ggcctactgg gacatccttg ccaagctgga tgccagcgtg tccgacccgg acgccgcgcc gcccaccttc agcgcccggc tgacggccgt ggctggccgg cgggtggcca ctggccgcga tttcgactcg ctgggcacgc tgcgcaaagg cgacgaagtc attgtgctcg acgaggggag cgcgaccgcg ttggccgcgg gcctggatgg cacgcagctg accgtggcct cggccgagga gaagccctac gcccggcgcc cgtacccgcc gttcatgacc tccacgctgc agcaagaggc cagccgcaag ctgcggttct ccgccgagcg gacgatgagc atcgcccagc ggctgtacga aaacggctac atcacctata tgcgtaccga ctccaccacg ctgtcggagt cggcgatcaa cgccgcacgt acccaggcgc gccagctcta cggcgacgag tacgtcgcgc cggcgccgcg ccaatacacc cgcaaggtga agaacgccca ggaagcgcac gaggctatcc ggcccgccgg tgaaacgttt gccaccccgg acgcggtgcg tcgcgaactc gacggtccca acattgatga tttccggctc tatgagctga tttggcaacg caccgtagcc tcgcagatgg ccgatgcgcg gggcatgacg ctgagcctgc ggatcactgg catgtcgggg caccaggagg tggtgttctc cgcgaccgga cgcaccttga cgttcccggg cttcctcaag gcctacgtgg agaccgtgga cgagctggtc ggcggcgagg ctgacgatgc cgagcggcga ctgccccatc tgaccccggg tcaacggttg gacatcgtcg agttgacccc agacggccat gccaccaacc cgccggcccg ctacaccgag gcgtcgctgg tcaaagcgct cgaggagctg ggcatcggcc gcccgtcgac ctactcgtcg atcatcaaga ccatccagga tcgcggctac gtgcacaaga agggcagtgc actggtgccg tcatgggtgg cgttcgcggt aaccggtctg ctcgagcagc atttcggtcg gctcgtcgac tacgacttca ccgcggcgat ggaagacgag ctcgacgaga tcgccgccgg caacgagcgc cgcaccaact ggctcaacaa cttctacttt ggttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for MytuD.18062.a.B21.GE37955
HARVESTED ON: 4/15/2014
SEQUENCED ON: 4/16/2014
EXPECTED MW: 70kDa
OBSERVED MW: 70kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHLA DPKTKGRGSG GNGSGRRLVI VESPTKARKL ASYLGSGYIV ESSRGHIRDL PRAASDVPAK YKSQPWARLG VNVDADFEPL YIISPEKRST VSELRGLLKD VDELYLATDG DREGEAIAWH LLETLKPRIP VKRMVFHEIT EPAIRAAAEH PRDLDIDLVD AQETRRILDR LYGYEVSPVL WKKVAPKLSA GRVQSVATRI IVARERDRMA FRSAAYWDIL AKLDASVSDP DAAPPTFSAR LTAVAGRRVA TGRDFDSLGT LRKGDEVIVL DEGSATALAA GLDGTQLTVA SAEEKPYARR PYPPFMTSTL QQEASRKLRF SAERTMSIAQ RLYENGYITF MRTDSTTLSE SAINAARTQA RQLYGDEYVA PAPRQYTRKV KNAQEAHEAI RPAGETFATP DAVRRELDGP NIDDFRLYEL IWQRTVASQM ADARGMTLSL RITGMSGHQE VVFSATGRTL TFPGFLKAYV ETVDELVGGE ADDAERRLPH LTPGQRLDIV ELTPDGHATN PPARYTEASL VKALEELGIG RPSTYSSIIK TIQDRGYVHK KGSALVPSWV AFAVTGLLEQ HFGRLVDYDF TAAMEDELDE IAAGNERRTN WLNNFYFGGD HGVPDSVARS G
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttggctg acccgaaaac gaagggccgt ggcagcggcg gcaatggcag cggccggcga ctggtcatcg tcgagtcgcc caccaaggcg cgcaagctgg cctcctacct gggctctggc tacatcgtcg agtcctcccg ggggcacatc cgtgacttgc cgcgggccgc gtcggatgta cccgcaaagt acaagtcgca gccgtgggcg cggctcgggg tcaacgtcga cgccgacttc gaaccgctct acatcatcag cccggagaaa cggagcaccg tcagcgagct caggggcctg ctcaaagacg tggacgagct gtatctggcc acggatgggg accgtgaggg cgaagctatt gcctggcatc tgctggaaac cctcaaaccg cgcataccgg taaagcggat ggtcttccac gagatcaccg aaccggcgat ccgcgccgcc gccgagcacc cccgcgacct agacatcgac ctggtcgacg cgcaggagac ccggcgcatc ctggaccggc tgtacggcta cgaagtcagc ccagtgctgt ggaagaaggt cgcccccaag ttgtcggcgg gccgggtgca gtcggtggcc acccgcatca tcgtggcgcg cgaacgcgac cgcatggcgt tccgcagcgc ggcctactgg gacatccttg ccaagctgga tgccagcgtg tccgacccgg acgccgcgcc gcccaccttc agcgcccggc tgacggccgt ggctggccgg cgggtggcca ctggccgcga tttcgactcg ctgggcacgc tgcgcaaagg cgacgaagtc attgtgctcg acgaggggag cgcgaccgcg ttggccgcgg gcctggatgg cacgcagctg accgtggcct cggccgagga gaagccctac gcccggcgcc cgtacccgcc gttcatgacc tccacgctgc agcaagaggc cagccgcaag ctgcggttct ccgccgagcg gacgatgagc atcgcccagc ggctgtacga aaacggctac atcaccttta tgcgtaccga ctccaccacg ctgtcggagt cggcgatcaa cgccgcacgt acccaggcgc gccagctcta cggcgacgag tacgtcgcgc cggcgccgcg ccaatacacc cgcaaggtga agaacgccca ggaagcgcac gaggctatcc ggcccgccgg tgaaacgttt gccaccccgg acgcggtgcg tcgcgaactc gacggtccca acattgatga tttccggctc tatgagctga tttggcaacg caccgtagcc tcgcagatgg ccgatgcgcg gggcatgacg ctgagcctgc ggatcactgg catgtcgggg caccaggagg tggtgttctc cgcgaccgga cgcaccttga cgttcccggg cttcctcaag gcctacgtgg agaccgtgga cgagctggtc ggcggcgagg ctgacgatgc cgagcggcga ctgccccatc tgaccccggg tcaacggttg gacatcgtcg agttgacccc agacggccat gccaccaacc cgccggcccg ctacaccgag gcgtcgctgg tcaaagcgct cgaggagctg ggcatcggcc gcccgtcgac ctactcgtcg atcatcaaga ccatccagga tcgcggctac gtgcacaaga agggcagtgc actggtgccg tcatgggtgg cgttcgcggt aaccggtctg ctcgagcagc atttcggtcg gctcgtcgac tacgacttca ccgcggcgat ggaagacgag ctcgacgaga tcgccgccgg caacgagcgc cgcaccaact ggctcaacaa cttctacttt ggtggcgatc acggtgtgcc cgattcggta gcccgatcgg gttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for MytuD.18062.a.B31.GE37956
HARVESTED ON: 4/15/2014
SEQUENCED ON: 4/18/2014
EXPECTED MW: 69kDa
OBSERVED MW: 69kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 89
PERCENT COVERAGE: 66
Validated AA Sequence
MAHHHHHHML ADPKTKGRGS GGNGSGRRLV IVESPTKARK LASYLGSGYI VESSRGHIRD LPRAASDVPA KYKSQPWARL GVNVDADFEP LYIISPEKRS TVSELRGLLK DVDELYLATD GDREGEAIAW HLLETLKPRI PVKRMVFHEI TEPAIRAAAE HPRDLDIDLV DAQETRRILD RLYGYEVSPV LWKKVAPKLS AGRVQSVATR IIVARERDRM AFRSAAYWDI LAKLDASVSD PDAAPPTFSA RLTAVAGRRV ATGRDFDSLG TLRKGDEVIV LDEGSATALA AGLDGTQLTV ASAEEKPYAR RPYPPFMTST LQQEASRKLR FSAERTMSIA QRLYENGYXT FMRTDSXRCR SRRSTPHGTQ AXXLXRAXXY VXPRPXXPXX XXXXXXEXXP XRVKXXXXP
Validated NT Sequence
gnnnntnnnn nnnnnnttnt tgtttaactt taagaaggag atataccatg gctcaccacc accaccacca tatgttggct gacccgaaaa cgaagggccg tggcagcggc ggcaatggca gcggccggcg actggtcatc gtcgagtcgc ccaccaaggc gcgcaagctg gcctcctacc tgggctctgg ctacatcgtc gagtcctccc gggggcacat ccgtgacttg ccgcgggccg cgtcggatgt acccgcaaag tacaagtcgc agccgtgggc gcggctcggg gtcaacgtcg acgccgactt cgaaccgctc tacatcatca gcccggagaa acggagcacc gtcagcgagc tcaggggcct gctcaaagac gtggacgagc tgtatctggc cacggatggg gaccgtgagg gcgaagctat tgcctggcat ctgctggaaa ccctcaaacc gcgcataccg gtaaagcgga tggtcttcca cgagatcacc gaaccggcga tccgcgccgc cgccgagcac ccccgcgacc tagacatcga cctggtcgac gcgcaggaga cccggcgcat cctggaccgg ctgtacggct acgaagtcag cccagtgctg tggaagaagg tcgcccccaa gttgtcggcg ggccgggtgc agtcggtggc cacccgcatc atcgtggcgc gcgaacgcga ccgcatggcg ttccgcagcg cggcctactg ggacatcctt gccaagctgg atgccagcgt gtccgacccg gacgccgcgc cgcccacctt cagcgcccgg ctgacggccg tggctggccg gcgggtggcc actggccgcg atttcgactc gctgggcacg ctgcgcaaag gcgacgaagt cattgtgctc gacgagggga gcgcgaccgc gttggccgcg ggcctggatg gcacgcagct gaccgtggcc tcggccgagg agaagcccta cgcccggcgc ccgtacccgc cgttcatgac ctccacgctg cagcaagagg ccagccgcaa gctgcggttc tccgccgagc ggacgatgag catcgcccag cggctgtacg aaaacggcta cntcaccttt atgcgtaccg actccncacg ctgtcggagt cggcgatcaa cgccgcacgg tacccaggcg ngccanctct tncgggcnnc nangtacgtc nnnccncgnc cnntnnnncc nncnnnnnna nnncnnnnnn nnnangagcn tnnncccnnc cgggtgaaan nnnnncnnnn ccccc
Expected Protein Sequence
MAHHHHHHLA DPKTKGRGSG GNGSGRRLVI VESPTKARKL ASYLGSGYIV ESSRGHIRDL PRAASDVPAK YKSQPWARLG VNVDADFEPL YIISPEKRST VSELRGLLKD VDELYLATDG DREGEAIAWH LLETLKPRIP VKRMVFHEIT EPAIRAAAEH PRDLDIDLVD AQETRRILDR LYGYEVSPVL WKKVAPKLSA GRVQSVATRI IVARERDRMA FRSAAYWDIL AKLDASVSDP DAAPPTFSAR LTAVAGRRVA TGRDFDSLGT LRKGDEVIVL DEGSATALAA GLDGTQLTVA SAEEKPYARR PYPPFMTSTL QQEASRKLRF SAERTMSIAQ RLYENGYITF MRTDSTTLSE SAINAARTQA RQLYGDEYVA PAPRQYTRKV KNAQEAHEAI RPAGETFATP DAVRRELDGP NIDDFRLYEL IWQRTVASQM ADARGMTLSL RITGMSGHQE VVFSATGRTL TFPGFLKAYV ETVDELVGGE ADDAERRLPH LTPGQRLDIV ELTPDGHATN PPARYTEASL VKALEELGIG RPSTYSSIIK TIQDRGYVHK KGSALVPSWV AFAVTGLLEQ HFGRLVDYDF TAAMEDELDE IAAGNERRTN WLNNFYFG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttggctg acccgaaaac gaagggccgt ggcagcggcg gcaatggcag cggccggcga ctggtcatcg tcgagtcgcc caccaaggcg cgcaagctgg cctcctacct gggctctggc tacatcgtcg agtcctcccg ggggcacatc cgtgacttgc cgcgggccgc gtcggatgta cccgcaaagt acaagtcgca gccgtgggcg cggctcgggg tcaacgtcga cgccgacttc gaaccgctct acatcatcag cccggagaaa cggagcaccg tcagcgagct caggggcctg ctcaaagacg tggacgagct gtatctggcc acggatgggg accgtgaggg cgaagctatt gcctggcatc tgctggaaac cctcaaaccg cgcataccgg taaagcggat ggtcttccac gagatcaccg aaccggcgat ccgcgccgcc gccgagcacc cccgcgacct agacatcgac ctggtcgacg cgcaggagac ccggcgcatc ctggaccggc tgtacggcta cgaagtcagc ccagtgctgt ggaagaaggt cgcccccaag ttgtcggcgg gccgggtgca gtcggtggcc acccgcatca tcgtggcgcg cgaacgcgac cgcatggcgt tccgcagcgc ggcctactgg gacatccttg ccaagctgga tgccagcgtg tccgacccgg acgccgcgcc gcccaccttc agcgcccggc tgacggccgt ggctggccgg cgggtggcca ctggccgcga tttcgactcg ctgggcacgc tgcgcaaagg cgacgaagtc attgtgctcg acgaggggag cgcgaccgcg ttggccgcgg gcctggatgg cacgcagctg accgtggcct cggccgagga gaagccctac gcccggcgcc cgtacccgcc gttcatgacc tccacgctgc agcaagaggc cagccgcaag ctgcggttct ccgccgagcg gacgatgagc atcgcccagc ggctgtacga aaacggctac atcaccttta tgcgtaccga ctccaccacg ctgtcggagt cggcgatcaa cgccgcacgt acccaggcgc gccagctcta cggcgacgag tacgtcgcgc cggcgccgcg ccaatacacc cgcaaggtga agaacgccca ggaagcgcac gaggctatcc ggcccgccgg tgaaacgttt gccaccccgg acgcggtgcg tcgcgaactc gacggtccca acattgatga tttccggctc tatgagctga tttggcaacg caccgtagcc tcgcagatgg ccgatgcgcg gggcatgacg ctgagcctgc ggatcactgg catgtcgggg caccaggagg tggtgttctc cgcgaccgga cgcaccttga cgttcccggg cttcctcaag gcctacgtgg agaccgtgga cgagctggtc ggcggcgagg ctgacgatgc cgagcggcga ctgccccatc tgaccccggg tcaacggttg gacatcgtcg agttgacccc agacggccat gccaccaacc cgccggcccg ctacaccgag gcgtcgctgg tcaaagcgct cgaggagctg ggcatcggcc gcccgtcgac ctactcgtcg atcatcaaga ccatccagga tcgcggctac gtgcacaaga agggcagtgc actggtgccg tcatgggtgg cgttcgcggt aaccggtctg ctcgagcagc atttcggtcg gctcgtcgac tacgacttca ccgcggcgat ggaagacgag ctcgacgaga tcgccgccgg caacgagcgc cgcaccaact ggctcaacaa cttctacttt ggttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for MytuD.18062.a.B11.PW37435
PURIFICATION DATe: 1/29/2014
CONCENTRATION: 33.1mg/ml
OBSERVED MW: 103kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 110µl
PERCENT IDENTITY: 85
PERCENT COVERAGE: 42
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML ADPKTKGRGS GGNGSGRRLV IVESPTKARK LASYLGSGYI VESSRGHIRD LPRAASDVPA KYKSQPWARL GVNVDADFEP LYIISPEKRS TVSELRGLLK DVDELYLATD GDREGEAIAW HLLETLKPRI PVKRMVFHEI TEPAIRAAAE HPRDLDIDLV DAQETRRILD RLYGYEVSPV LWKKVAPKLS AGRVQSVATR IIVARERDRM AFRSAAYWDI LAKLDASVSD PDAAPPTFSA RLTAVAGRRV ATGRDFDSLG TLRKGDEVIV LDEGSATALA AGLDGTQLTV ASAEEKPYAR RPYPPFMTST LQQEAAQAAV XRRADDEHRP XAVRKXLXXL YGVRLHPPXX XXXDQXXXXX XXXXXXXXXX XXXXXXXHXX XXXXXXXXX
Validated NT Sequence
aggnncncta naannntttt gtttncttta agaaggagat ataccatggc tcaccaccac caccaccata tgttggctga cccgaaaacg aagggccgtg gcagcggcgg caatggcagc ggccggcgac tggtcatcgt cgagtcgccc accaaggcgc gcaagctggc ctcctacctg ggctctggct acatcgtcga gtcctcccgg gggcacatcc gtgacttgcc gcgggccgcg tcggatgtac ccgcaaagta caagtcgcag ccgtgggcgc ggctcggggt caacgtcgac gccgacttcg aaccgctcta catcatcagc ccggagaaac ggagcaccgt cagcgagctc aggggcctgc tcaaagacgt ggacgagctg tatctggcca cggatgggga ccgtgagggc gaagctattg cctggcatct gctggaaacc ctcaaaccgc gcataccggt aaagcggatg gtcttccacg agatcaccga accggcgatc cgcgccgccg ccgagcaccc ccgcgaccta gacatcgacc tggtcgacgc gcaggagacc cggcgcatcc tggaccggct gtacggctac gaagtcagcc cagtgctgtg gaagaaggtc gcccccaagt tgtcggcggg ccgggtgcag tcggtggcca cccgcatcat cgtggcgcgc gaacgcgacc gcatggcgtt ccgcagcgcg gcctactggg acatccttgc caagctggat gccagcgtgt ccgacccgga cgccgcgccg cccaccttca gcgcccggct gacggccgtg gctggccggc gggtggccac tggccgcgat ttcgactcgc tgggcacgct gcgcaaaggc gacgaagtca ttgtgctcga cgaggggagc gcgaccgcgt tggccgcggg cctggatggc acgcagctga ccgtggcctc ggccgaggag aagccctacg cccggcgccc gtacccgccg ttcatgacct ccacgctgca gcaagaggca gcgcaagctg cggtnntccg ccgagcggac gatgagcatc gcccancggc tgtacgaaaa cngctanntc anctttatgg cgtacgactc cacccnccnn tgntcgnnnn ngncgatcaa ngccnnnacg ntnccnnggn nnnnnnnnnt nnngnnnnnn natanntnnn gncnnnnnnn nnnntncacn nnnngnnana aannnnnnna anncnnnang ntt
Expressed Protein Sequence
MAHHHHHHLA DPKTKGRGSG GNGSGRRLVI VESPTKARKL ASYLGSGYIV ESSRGHIRDL PRAASDVPAK YKSQPWARLG VNVDADFEPL YIISPEKRST VSELRGLLKD VDELYLATDG DREGEAIAWH LLETLKPRIP VKRMVFHEIT EPAIRAAAEH PRDLDIDLVD AQETRRILDR LYGYEVSPVL WKKVAPKLSA GRVQSVATRI IVARERDRMA FRSAAYWDIL AKLDASVSDP DAAPPTFSAR LTAVAGRRVA TGRDFDSLGT LRKGDEVIVL DEGSATALAA GLDGTQLTVA SAEEKPYARR PYPPFMTSTL QQEASRKLRF SAERTMSIAQ RLYENGYITY MRTDSTTLSE SAINAARTQA RQLYGDEYVA PAPRQYTRKV KNAQEAHEAI RPAGETFATP DAVRRELDGP NIDDFRLYEL IWQRTVASQM ADARGMTLSL RITGMSGHQE VVFSATGRTL TFPGFLKAYV ETVDELVGGE ADDAERRLPH LTPGQRLDIV ELTPDGHATN PPARYTEASL VKALEELGIG RPSTYSSIIK TIQDRGYVHK KGSALVPSWV AFAVTGLLEQ HFGRLVDYDF TAAMEDELDE IAAGNERRTN WLNNFYFGGD HGVPDSVARS GGLKKLVGIN LEGIDAREVN SIKLFDDTHG RPIYVRVGKN GPYLERLVAG DTGEPTPQRA NLSDSITPDE LTLQVAEELF ATPQQGRTLG LDPETGHEIV AREGRFGPYV TEILPEPAAD AAAAAQGVKK RQKAAGPKPR TGSLLRSMDL QTVTLEDALR LLSLPRVVGV DPASGEEITA QNGRYGPYLK RGNDSRSLVT EDQIFTITLD EALKIYAEPK RRGRQSASAP PLRELGTDPA SGKPMVIKDG RFGPYVTDGE TNASLRKGDD VASITDERAA ELLADRRARG PAKRPARKAA RKVPAKKAAK RD
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTTGGCTG ACCCGAAAAC GAAGGGCCGT GGCAGCGGCG GCAATGGCAG CGGCCGGCGA CTGGTCATCG TCGAGTCGCC CACCAAGGCG CGCAAGCTGG CCTCCTACCT GGGCTCTGGC TACATCGTCG AGTCCTCCCG GGGGCACATC CGTGACTTGC CGCGGGCCGC GTCGGATGTA CCCGCAAAGT ACAAGTCGCA GCCGTGGGCG CGGCTCGGGG TCAACGTCGA CGCCGACTTC GAACCGCTCT ACATCATCAG CCCGGAGAAA CGGAGCACCG TCAGCGAGCT CAGGGGCCTG CTCAAAGACG TGGACGAGCT GTATCTGGCC ACGGATGGGG ACCGTGAGGG CGAAGCTATT GCCTGGCATC TGCTGGAAAC CCTCAAACCG CGCATACCGG TAAAGCGGAT GGTCTTCCAC GAGATCACCG AACCGGCGAT CCGCGCCGCC GCCGAGCACC CCCGCGACCT AGACATCGAC CTGGTCGACG CGCAGGAGAC CCGGCGCATC CTGGACCGGC TGTACGGCTA CGAAGTCAGC CCAGTGCTGT GGAAGAAGGT CGCCCCCAAG TTGTCGGCGG GCCGGGTGCA GTCGGTGGCC ACCCGCATCA TCGTGGCGCG CGAACGCGAC CGCATGGCGT TCCGCAGCGC GGCCTACTGG GACATCCTTG CCAAGCTGGA TGCCAGCGTG TCCGACCCGG ACGCCGCGCC GCCCACCTTC AGCGCCCGGC TGACGGCCGT GGCTGGCCGG CGGGTGGCCA CTGGCCGCGA TTTCGACTCG CTGGGCACGC TGCGCAAAGG CGACGAAGTC ATTGTGCTCG ACGAGGGGAG CGCGACCGCG TTGGCCGCGG GCCTGGATGG CACGCAGCTG ACCGTGGCCT CGGCCGAGGA GAAGCCCTAC GCCCGGCGCC CGTACCCGCC GTTCATGACC TCCACGCTGC AGCAAGAGGC CAGCCGCAAG CTGCGGTTCT CCGCCGAGCG GACGATGAGC ATCGCCCAGC GGCTGTACGA AAACGGCTAC ATCACCTTTA TGCGTACCGA CTCCACCACG CTGTCGGAGT CGGCGATCAA CGCCGCACGT ACCCAGGCGC GCCAGCTCTA CGGCGACGAG TACGTCGCGC CGGCGCCGCG CCAATACACC CGCAAGGTGA AGAACGCCCA GGAAGCGCAC GAGGCTATCC GGCCCGCCGG TGAAACGTTT GCCACCCCGG ACGCGGTGCG TCGCGAACTC GACGGTCCCA ACATTGATGA TTTCCGGCTC TATGAGCTGA TTTGGCAACG CACCGTAGCC TCGCAGATGG CCGATGCGCG GGGCATGACG CTGAGCCTGC GGATCACTGG CATGTCGGGG CACCAGGAGG TGGTGTTCTC CGCGACCGGA CGCACCTTGA CGTTCCCGGG CTTCCTCAAG GCCTACGTGG AGACCGTGGA CGAGCTGGTC GGCGGCGAGG CTGACGATGC CGAGCGGCGA CTGCCCCATC TGACCCCGGG TCAACGGTTG GACATCGTCG AGTTGACCCC AGACGGCCAT GCCACCAACC CGCCGGCCCG CTACACCGAG GCGTCGCTGG TCAAAGCGCT CGAGGAGCTG GGCATCGGCC GCCCGTCGAC CTACTCGTCG ATCATCAAGA CCATCCAGGA TCGCGGCTAC GTGCACAAGA AGGGCAGTGC ACTGGTGCCG TCATGGGTGG CGTTCGCGGT AACCGGTCTG CTCGAGCAGC ATTTCGGTCG GCTCGTCGAC TACGACTTCA CCGCGGCGAT GGAAGACGAG CTCGACGAGA TCGCCGCCGG CAACGAGCGC CGCACCAACT GGCTCAACAA CTTCTACTTT GGTGGCGATC ACGGTGTGCC CGATTCGGTA GCCCGATCGG GTGGCCTCAA GAAGCTTGTC GGGATCAATC TCGAGGGCAT CGACGCACGA GAAGTAAACT CTATCAAGCT TTTTGACGAC ACCCACGGAC GCCCCATATA TGTTCGGGTG GGCAAGAACG GTCCCTACCT GGAACGTTTG GTGGCCGGCG ACACCGGTGA GCCCACGCCG CAGCGGGCCA ACCTCAGCGA CTCGATTACC CCGGACGAGC TGACTCTACA GGTGGCCGAA GAGCTCTTTG CCACACCGCA ACAGGGACGG ACTTTGGGCT TGGACCCAGA AACCGGCCAC GAGATCGTGG CCAGGGAAGG CCGGTTTGGG CCGTATGTGA CCGAGATCCT GCCGGAGCCT GCGGCTGATG CGGCCGCGGC CGCTCAGGGA GTCAAGAAAC GCCAGAAGGC CGCCGGGCCC AAACCGCGCA CCGGTTCGTT GCTGCGGAGC ATGGACCTAC AGACGGTCAC CCTCGAAGAC GCGCTGAGGC TGCTGTCACT GCCGCGCGTG GTCGGAGTGG ACCCCGCCTC GGGTGAGGAG ATCACCGCGC AGAACGGGCG CTACGGACCG TATCTAAAGC GCGGCAACGA TTCTCGATCA CTGGTCACCG AAGACCAGAT ATTCACCATC ACGCTCGACG AAGCCCTGAA GATCTACGCA GAGCCGAAAC GTCGTGGCCG GCAAAGCGCT TCGGCTCCGC CGCTGCGCGA GCTGGGAACA GATCCGGCGT CGGGCAAGCC AATGGTCATC AAGGACGGCC GATTCGGGCC GTACGTCACC GACGGTGAGA CCAATGCCAG CCTGCGTAAG GGCGACGACG TGGCTTCCAT AACCGACGAG CGCGCCGCCG AGCTGTTGGC CGATCGCCGA GCCCGGGGTC CGGCAAAACG GCCAGCCAGG AAAGCTGCCC GGAAGGTGCC GGCGAAGAAG GCAGCCAAGC GCGACTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT
Details for MytuD.18062.a.B2.PW37563
PURIFICATION DATe: 9/22/2014
CONCENTRATION: 25mg/ml
OBSERVED MW: 72kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 50
Protocol Notes
notes unavailable
Validated AA Sequence
MTXTLQQEXS RKLRFSAERT MSIAQRLYEN GYITYMRTDS TTLSESAINA ARTQARQLYG DEYVAPAPRQ YTRKVKNAQE AHEAIRPAGE TFATPDAVRR ELDGPNIDDF RLYELIWQRT VASQMADARG MTLSLRITGM SGHQEVVFSA TGRTLTFPGF LKAYVETVDE LVGGEADDAE RRLPHLTPGQ RLDIVELTPD GHATNPPARY TEASLVKALE ELGIGRPSTY SSIIKTIQDR GYVHKKGSAL VPSWVAFAVT GLLEQHFGRL VDYDFTAAME DELDEIAAGN ERRTNWLNNF YFGGDHGVPD SVARSG
Validated NT Sequence
atcttactca cttaacccga tcgggctacc gaatcgggca caccgtgatc gccaccaaag tagaagttgt tgagccagtt ggtgcggcgc tcgttgccgg cggcgatctc gtcgagctcg tcttccatcg ccgcggtgaa gtcgtagtcg acgagccgac cgaaatgctg ctcgagcaga ccggttaccg cgaacgccac ccatgacggc accagtgcac tgcccttctt gtgcacgtag ccgcgatcct ggatggtctt gatgatcgac gagtaggtcg acgggcggcc gatgcccagc tcctcgagcg ctttgaccag cgacgcctcg gtgtagcggg ccggcgggtt ggtggcatgg ccgtctgggg tcaactcgac gatgtccaac cgttgacccg gggtcagatg gggcagtcgc cgctcggcat cgtcagcctc gccgccgacc agctcgtcca cggtctccac gtaggccttg aggaagcccg ggaacgtcaa ggtgcgtccg gtcgcggaga acaccacctc ctggtgcccc gacatgccag tgatccgcag gctcagcgtc atgccccgcg catcggccat ctgcgaggct acggtgcgtt gccaaatcag ctcatagagc cggaaatcat caatgttggg accgtcgagt tcgcgacgca ccgcgtccgg ggtggcaaac gtttcaccgg cgggccggat agcctcgtgc gcttcctggg cgttcttcac cttgcgggtg tattggcgcg gcgccggcgc gacgtactcg tcgccgtaga gctggcgcgc ctgggtacgt gcggcgttga tcgccgactc cgacagcgtg gtggagtcgg tacgcatata ggtgatgtag ccgttttcgt acagccgctg ggcgatgctc atcgtccgct cggcggagaa ccgcagcttg cggctggnct cttgctgcag cgtggnngtc atgaacggcg ggtanngncn nnnggcgtnn gnntnntcnn nggccgaggc cacnntcagc tgc
Expressed Protein Sequence
MAHHHHHHMA DPKTKGRGSG GNGSGRRLVI VESPTKARKL ASYLGSGYIV ESSRGHIRDL PRAASDVPAK YKSQPWARLG VNVDADFEPL YIISPEKRST VSELRGLLKD VDELYLATDG DREGEAIAWH LLETLKPRIP VKRMVFHEIT EPAIRAAAEH PRDLDIDLVD AQETRRILDR LYGYEVSPVL WKKVAPKLSA GRVQSVATRI IVARERDRMA FRSAAYWDIL AKLDASVSDP DAAPPTFSAR LTAVAGRRVA TGRDFDSLGT LRKGDEVIVL DEGSATALAA GLDGTQLTVA SAEEKPYARR PYPPFMTSTL QQEASRKLRF SAERTMSIAQ RLYENGYITY MRTDSTTLSE SAINAARTQA RQLYGDEYVA PAPRQYTRKV KNAQEAHEAI RPAGETFATP DAVRRELDGP NIDDFRLYEL IWQRTVASQM ADARGMTLSL RITGMSGHQE VVFSATGRTL TFPGFLKAYV ETVDELVGGE ADDAERRLPH LTPGQRLDIV ELTPDGHATN PPARYTEASL VKALEELGIG RPSTYSSIIK TIQDRGYVHK KGSALVPSWV AFAVTGLLEQ HFGRLVDYDF TAAMEDELDE IAAGNERRTN WLNNFYFGGD HGVPDSVARS G
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTTGGCTG ACCCGAAAAC GAAGGGCCGT GGCAGCGGCG GCAATGGCAG CGGCCGGCGA CTGGTCATCG TCGAGTCGCC CACCAAGGCG CGCAAGCTGG CCTCCTACCT GGGCTCTGGC TACATCGTCG AGTCCTCCCG GGGGCACATC CGTGACTTGC CGCGGGCCGC GTCGGATGTA CCCGCAAAGT ACAAGTCGCA GCCGTGGGCG CGGCTCGGGG TCAACGTCGA CGCCGACTTC GAACCGCTCT ACATCATCAG CCCGGAGAAA CGGAGCACCG TCAGCGAGCT CAGGGGCCTG CTCAAAGACG TGGACGAGCT GTATCTGGCC ACGGATGGGG ACCGTGAGGG CGAAGCTATT GCCTGGCATC TGCTGGAAAC CCTCAAACCG CGCATACCGG TAAAGCGGAT GGTCTTCCAC GAGATCACCG AACCGGCGAT CCGCGCCGCC GCCGAGCACC CCCGCGACCT AGACATCGAC CTGGTCGACG CGCAGGAGAC CCGGCGCATC CTGGACCGGC TGTACGGCTA CGAAGTCAGC CCAGTGCTGT GGAAGAAGGT CGCCCCCAAG TTGTCGGCGG GCCGGGTGCA GTCGGTGGCC ACCCGCATCA TCGTGGCGCG CGAACGCGAC CGCATGGCGT TCCGCAGCGC GGCCTACTGG GACATCCTTG CCAAGCTGGA TGCCAGCGTG TCCGACCCGG ACGCCGCGCC GCCCACCTTC AGCGCCCGGC TGACGGCCGT GGCTGGCCGG CGGGTGGCCA CTGGCCGCGA TTTCGACTCG CTGGGCACGC TGCGCAAAGG CGACGAAGTC ATTGTGCTCG ACGAGGGGAG CGCGACCGCG TTGGCCGCGG GCCTGGATGG CACGCAGCTG ACCGTGGCCT CGGCCGAGGA GAAGCCCTAC GCCCGGCGCC CGTACCCGCC GTTCATGACC TCCACGCTGC AGCAAGAGGC CAGCCGCAAG CTGCGGTTCT CCGCCGAGCG GACGATGAGC ATCGCCCAGC GGCTGTACGA AAACGGCTAC ATCACCTATA TGCGTACCGA CTCCACCACG CTGTCGGAGT CGGCGATCAA CGCCGCACGT ACCCAGGCGC GCCAGCTCTA CGGCGACGAG TACGTCGCGC CGGCGCCGCG CCAATACACC CGCAAGGTGA AGAACGCCCA GGAAGCGCAC GAGGCTATCC GGCCCGCCGG TGAAACGTTT GCCACCCCGG ACGCGGTGCG TCGCGAACTC GACGGTCCCA ACATTGATGA TTTCCGGCTC TATGAGCTGA TTTGGCAACG CACCGTAGCC TCGCAGATGG CCGATGCGCG GGGCATGACG CTGAGCCTGC GGATCACTGG CATGTCGGGG CACCAGGAGG TGGTGTTCTC CGCGACCGGA CGCACCTTGA CGTTCCCGGG CTTCCTCAAG GCCTACGTGG AGACCGTGGA CGAGCTGGTC GGCGGCGAGG CTGACGATGC CGAGCGGCGA CTGCCCCATC TGACCCCGGG TCAACGGTTG GACATCGTCG AGTTGACCCC AGACGGCCAT GCCACCAACC CGCCGGCCCG CTACACCGAG GCGTCGCTGG TCAAAGCGCT CGAGGAGCTG GGCATCGGCC GCCCGTCGAC CTACTCGTCG ATCATCAAGA CCATCCAGGA TCGCGGCTAC GTGCACAAGA AGGGCAGTGC ACTGGTGCCG TCATGGGTGG CGTTCGCGGT AACCGGTCTG CTCGAGCAGC ATTTCGGTCG GCTCGTCGAC TACGACTTCA CCGCGGCGAT GGAAGACGAG CTCGACGAGA TCGCCGCCGG CAACGAGCGC CGCACCAACT GGCTCAACAA CTTCTACTTT GGTGGCGATC ACGGTGTGCC CGATTCGGTA GCCCGATCGG GTTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT
Details for MytuD.18062.a.B21.PW37505
PURIFICATION DATe: 6/11/2014
CONCENTRATION: 19.7mg/ml
OBSERVED MW: 73kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHLA DPKTKGRGSG GNGSGRRLVI VESPTKARKL ASYLGSGYIV ESSRGHIRDL PRAASDVPAK YKSQPWARLG VNVDADFEPL YIISPEKRST VSELRGLLKD VDELYLATDG DREGEAIAWH LLETLKPRIP VKRMVFHEIT EPAIRAAAEH PRDLDIDLVD AQETRRILDR LYGYEVSPVL WKKVAPKLSA GRVQSVATRI IVARERDRMA FRSAAYWDIL AKLDASVSDP DAAPPTFSAR LTAVAGRRVA TGRDFDSLGT LRKGDEVIVL DEGSATALAA GLDGTQLTVA SAEEKPYARR PYPPFMTSTL QQEASRKLRF SAERTMSIAQ RLYENGYITF MRTDSTTLSE SAINAARTQA RQLYGDEYVA PAPRQYTRKV KNAQEAHEAI RPAGETFATP DAVRRELDGP NIDDFRLYEL IWQRTVASQM ADARGMTLSL RITGMSGHQE VVFSATGRTL TFPGFLKAYV ETVDELVGGE ADDAERRLPH LTPGQRLDIV ELTPDGHATN PPARYTEASL VKALEELGIG RPSTYSSIIK TIQDRGYVHK KGSALVPSWV AFAVTGLLEQ HFGRLVDYDF TAAMEDELDE IAAGNERRTN WLNNFYFGGD HGVPDSVARS G
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTTGGCTG ACCCGAAAAC GAAGGGCCGT GGCAGCGGCG GCAATGGCAG CGGCCGGCGA CTGGTCATCG TCGAGTCGCC CACCAAGGCG CGCAAGCTGG CCTCCTACCT GGGCTCTGGC TACATCGTCG AGTCCTCCCG GGGGCACATC CGTGACTTGC CGCGGGCCGC GTCGGATGTA CCCGCAAAGT ACAAGTCGCA GCCGTGGGCG CGGCTCGGGG TCAACGTCGA CGCCGACTTC GAACCGCTCT ACATCATCAG CCCGGAGAAA CGGAGCACCG TCAGCGAGCT CAGGGGCCTG CTCAAAGACG TGGACGAGCT GTATCTGGCC ACGGATGGGG ACCGTGAGGG CGAAGCTATT GCCTGGCATC TGCTGGAAAC CCTCAAACCG CGCATACCGG TAAAGCGGAT GGTCTTCCAC GAGATCACCG AACCGGCGAT CCGCGCCGCC GCCGAGCACC CCCGCGACCT AGACATCGAC CTGGTCGACG CGCAGGAGAC CCGGCGCATC CTGGACCGGC TGTACGGCTA CGAAGTCAGC CCAGTGCTGT GGAAGAAGGT CGCCCCCAAG TTGTCGGCGG GCCGGGTGCA GTCGGTGGCC ACCCGCATCA TCGTGGCGCG CGAACGCGAC CGCATGGCGT TCCGCAGCGC GGCCTACTGG GACATCCTTG CCAAGCTGGA TGCCAGCGTG TCCGACCCGG ACGCCGCGCC GCCCACCTTC AGCGCCCGGC TGACGGCCGT GGCTGGCCGG CGGGTGGCCA CTGGCCGCGA TTTCGACTCG CTGGGCACGC TGCGCAAAGG CGACGAAGTC ATTGTGCTCG ACGAGGGGAG CGCGACCGCG TTGGCCGCGG GCCTGGATGG CACGCAGCTG ACCGTGGCCT CGGCCGAGGA GAAGCCCTAC GCCCGGCGCC CGTACCCGCC GTTCATGACC TCCACGCTGC AGCAAGAGGC CAGCCGCAAG CTGCGGTTCT CCGCCGAGCG GACGATGAGC ATCGCCCAGC GGCTGTACGA AAACGGCTAC ATCACCTTTA TGCGTACCGA CTCCACCACG CTGTCGGAGT CGGCGATCAA CGCCGCACGT ACCCAGGCGC GCCAGCTCTA CGGCGACGAG TACGTCGCGC CGGCGCCGCG CCAATACACC CGCAAGGTGA AGAACGCCCA GGAAGCGCAC GAGGCTATCC GGCCCGCCGG TGAAACGTTT GCCACCCCGG ACGCGGTGCG TCGCGAACTC GACGGTCCCA ACATTGATGA TTTCCGGCTC TATGAGCTGA TTTGGCAACG CACCGTAGCC TCGCAGATGG CCGATGCGCG GGGCATGACG CTGAGCCTGC GGATCACTGG CATGTCGGGG CACCAGGAGG TGGTGTTCTC CGCGACCGGA CGCACCTTGA CGTTCCCGGG CTTCCTCAAG GCCTACGTGG AGACCGTGGA CGAGCTGGTC GGCGGCGAGG CTGACGATGC CGAGCGGCGA CTGCCCCATC TGACCCCGGG TCAACGGTTG GACATCGTCG AGTTGACCCC AGACGGCCAT GCCACCAACC CGCCGGCCCG CTACACCGAG GCGTCGCTGG TCAAAGCGCT CGAGGAGCTG GGCATCGGCC GCCCGTCGAC CTACTCGTCG ATCATCAAGA CCATCCAGGA TCGCGGCTAC GTGCACAAGA AGGGCAGTGC ACTGGTGCCG TCATGGGTGG CGTTCGCGGT AACCGGTCTG CTCGAGCAGC ATTTCGGTCG GCTCGTCGAC TACGACTTCA CCGCGGCGAT GGAAGACGAG CTCGACGAGA TCGCCGCCGG CAACGAGCGC CGCACCAACT GGCTCAACAA CTTCTACTTT GGTGGCGATC ACGGTGTGCC CGATTCGGTA GCCCGATCGG GTTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT
Details for MytuD.18062.a.B3.PW37565
PURIFICATION DATe: 9/22/2014
CONCENTRATION: 25mg/ml
OBSERVED MW: 70kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 110µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 76
Protocol Notes
notes unavailable
Validated AA Sequence
MVFHEITEPA IRAAAEHPRD LDIDLVDAQE TRRILDRLYG YEVSPVLWKK VAPKLSAGRV QSVATRIIVA RERDRMAFRS AAYWDILAKL DASVSXPDAA PPTFSARLTA VAGRRVATGR DFDSLGTLRK GDEVIVLDEG SATALAAGLD GTQLTVASAE EKPYARRXYP PFMTSTLQQE ASRKLRFSAE RTMSIAQRLY ENGYITYMRT DSTTLSESAI NAARTQARQL YGDEYVAPAP RQYTRKVKNA QEAHEAIRPA GETFATPDAV RRELDGPNID DFRLYELIWQ RTVASQMADA RGMTLSLRIT GMSGHQEVVF SATGRTLTFP GFLKAYVETV DELVGGEADD AERRLPHLTP GQRLDIVELT PDGHATNPPA RYTEASLVKA LEELGIGRPS TYSSIIKTIQ DRGYVHKKGS ALVPSWVAFA VTGLLEQHFG RLVDYDFTAA MEDELDEIAA GNERRTNWLN NFYFG
Validated NT Sequence
atcttactca cttaaccaaa gtagaagttg ttgagccagt tggtgcggcg ctcgttgccg gcggcgatct cgtcgagctc gtcttccatc gccgcggtga agtcgtagtc gacgagccga ccgaaatgct gctcgagcag accggttacc gcgaacgcca cccatgacgg caccagtgca ctgcccttct tgtgcacgta gccgcgatcc tggatggtct tgatgatcga cgagtaggtc gacgggcggc cgatgcccag ctcctcgagc gctttgacca gcgacgcctc ggtgtagcgg gccggcgggt tggtggcatg gccgtctggg gtcaactcga cgatgtccaa ccgttgaccc ggggtcagat ggggcagtcg ccgctcggca tcgtcagcct cgccgccgac cagctcgtcc acggtctcca cgtaggcctt gaggaagccc gggaacgtca aggtgcgtcc ggtcgcggag aacaccacct cctggtgccc cgacatgcca gtgatccgca ggctcagcgt catgccccgc gcatcggcca tctgcgaggc tacggtgcgt tgccaaatca gctcatagag ccggaaatca tcaatgttgg gaccgtcgag ttcgcgacgc accgcgtccg gggtggcaaa cgtttcaccg gcgggccgga tagcctcgtg cgcttcctgg gcgttcttca ccttgcgggt gtattggcgc ggcgccggcg cgacgtactc gtcgccgtag agctggcgcg cctgggtacg tgcggcgttg atcgccgact ccgacagcgt ggtggagtcg gtacgcatat aggtgatgta gccgttttcg tacagccgct gggcgatgct catcgtccgc tcggcggaga accgcagctt gcggctggcc tcttgctgca gcgtggaggt catgaacggc gggtacnngc gccgggcgta gggcttctcc tcggccgagg ccacggtcag ctgcgtgcca tccaggcccg cggccaacgc ggtcgcgctc ccctcgtcga gcacaatgac ttcgtcgcct ttgcgcagcg tgcccagcga gtcgaaatcg cggccagtgg ccacccgccg nccagccacg gccgtcagcc gggcgctgaa ggtgggcggc gcggcgtccg gntcggacac gctggcatcc agcttggcaa ggatgtccca gtaggccgcg ctgcggaacg ccatgcggtc gcgttcgcgc gccacgatga tgcgggtggc caccgactgc acccggcccg ccgacaactt gggggcgacc ttcttccaca gcactgggct gacttcgtag ccgtacagcc ggtccaggat gcgccgggtc tcctgcgcgt cgaccaggtc gatgtctagg tcgcgggggt gctcggcggc ggcgcggatc gccggttcgg tgatctcgtg gaagaccatc cgctttaccg gtatgcgcgg tttgagggtt tccagcagat gccaggcaat agcttcgccc tcacggtccc catccgtggc cagatacagc tcgtccacgt ctttgagcag gcccctgagc tcgctgacgg tgctccgttt ctccgggctg atgatgtaga gcggttcgaa gtcggcgtcg acgttgaccc cgagccgcgc ccacggctgc gacttgtact ttgcgggtac atccgacgcg gcccgcggca agtcacggat gtgcccccgg gaggactcga cgatgtagcc agagcccagg taggaggcca gcttgcgcgc cttggtgggc gactcgacga tgaccagtcg ccggccgctg ccattgccgc cgctgccacg gcccttcgtt ttcgggtcag cc
Expressed Protein Sequence
MAHHHHHHMA DPKTKGRGSG GNGSGRRLVI VESPTKARKL ASYLGSGYIV ESSRGHIRDL PRAASDVPAK YKSQPWARLG VNVDADFEPL YIISPEKRST VSELRGLLKD VDELYLATDG DREGEAIAWH LLETLKPRIP VKRMVFHEIT EPAIRAAAEH PRDLDIDLVD AQETRRILDR LYGYEVSPVL WKKVAPKLSA GRVQSVATRI IVARERDRMA FRSAAYWDIL AKLDASVSDP DAAPPTFSAR LTAVAGRRVA TGRDFDSLGT LRKGDEVIVL DEGSATALAA GLDGTQLTVA SAEEKPYARR PYPPFMTSTL QQEASRKLRF SAERTMSIAQ RLYENGYITY MRTDSTTLSE SAINAARTQA RQLYGDEYVA PAPRQYTRKV KNAQEAHEAI RPAGETFATP DAVRRELDGP NIDDFRLYEL IWQRTVASQM ADARGMTLSL RITGMSGHQE VVFSATGRTL TFPGFLKAYV ETVDELVGGE ADDAERRLPH LTPGQRLDIV ELTPDGHATN PPARYTEASL VKALEELGIG RPSTYSSIIK TIQDRGYVHK KGSALVPSWV AFAVTGLLEQ HFGRLVDYDF TAAMEDELDE IAAGNERRTN WLNNFYFG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTTGGCTG ACCCGAAAAC GAAGGGCCGT GGCAGCGGCG GCAATGGCAG CGGCCGGCGA CTGGTCATCG TCGAGTCGCC CACCAAGGCG CGCAAGCTGG CCTCCTACCT GGGCTCTGGC TACATCGTCG AGTCCTCCCG GGGGCACATC CGTGACTTGC CGCGGGCCGC GTCGGATGTA CCCGCAAAGT ACAAGTCGCA GCCGTGGGCG CGGCTCGGGG TCAACGTCGA CGCCGACTTC GAACCGCTCT ACATCATCAG CCCGGAGAAA CGGAGCACCG TCAGCGAGCT CAGGGGCCTG CTCAAAGACG TGGACGAGCT GTATCTGGCC ACGGATGGGG ACCGTGAGGG CGAAGCTATT GCCTGGCATC TGCTGGAAAC CCTCAAACCG CGCATACCGG TAAAGCGGAT GGTCTTCCAC GAGATCACCG AACCGGCGAT CCGCGCCGCC GCCGAGCACC CCCGCGACCT AGACATCGAC CTGGTCGACG CGCAGGAGAC CCGGCGCATC CTGGACCGGC TGTACGGCTA CGAAGTCAGC CCAGTGCTGT GGAAGAAGGT CGCCCCCAAG TTGTCGGCGG GCCGGGTGCA GTCGGTGGCC ACCCGCATCA TCGTGGCGCG CGAACGCGAC CGCATGGCGT TCCGCAGCGC GGCCTACTGG GACATCCTTG CCAAGCTGGA TGCCAGCGTG TCCGACCCGG ACGCCGCGCC GCCCACCTTC AGCGCCCGGC TGACGGCCGT GGCTGGCCGG CGGGTGGCCA CTGGCCGCGA TTTCGACTCG CTGGGCACGC TGCGCAAAGG CGACGAAGTC ATTGTGCTCG ACGAGGGGAG CGCGACCGCG TTGGCCGCGG GCCTGGATGG CACGCAGCTG ACCGTGGCCT CGGCCGAGGA GAAGCCCTAC GCCCGGCGCC CGTACCCGCC GTTCATGACC TCCACGCTGC AGCAAGAGGC CAGCCGCAAG CTGCGGTTCT CCGCCGAGCG GACGATGAGC ATCGCCCAGC GGCTGTACGA AAACGGCTAC ATCACCTATA TGCGTACCGA CTCCACCACG CTGTCGGAGT CGGCGATCAA CGCCGCACGT ACCCAGGCGC GCCAGCTCTA CGGCGACGAG TACGTCGCGC CGGCGCCGCG CCAATACACC CGCAAGGTGA AGAACGCCCA GGAAGCGCAC GAGGCTATCC GGCCCGCCGG TGAAACGTTT GCCACCCCGG ACGCGGTGCG TCGCGAACTC GACGGTCCCA ACATTGATGA TTTCCGGCTC TATGAGCTGA TTTGGCAACG CACCGTAGCC TCGCAGATGG CCGATGCGCG GGGCATGACG CTGAGCCTGC GGATCACTGG CATGTCGGGG CACCAGGAGG TGGTGTTCTC CGCGACCGGA CGCACCTTGA CGTTCCCGGG CTTCCTCAAG GCCTACGTGG AGACCGTGGA CGAGCTGGTC GGCGGCGAGG CTGACGATGC CGAGCGGCGA CTGCCCCATC TGACCCCGGG TCAACGGTTG GACATCGTCG AGTTGACCCC AGACGGCCAT GCCACCAACC CGCCGGCCCG CTACACCGAG GCGTCGCTGG TCAAAGCGCT CGAGGAGCTG GGCATCGGCC GCCCGTCGAC CTACTCGTCG ATCATCAAGA CCATCCAGGA TCGCGGCTAC GTGCACAAGA AGGGCAGTGC ACTGGTGCCG TCATGGGTGG CGTTCGCGGT AACCGGTCTG CTCGAGCAGC ATTTCGGTCG GCTCGTCGAC TACGACTTCA CCGCGGCGAT GGAAGACGAG CTCGACGAGA TCGCCGCCGG CAACGAGCGC CGCACCAACT GGCTCAACAA CTTCTACTTT GGTTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT
Details for MytuD.18062.a.B3.PW37432
PURIFICATION DATe: 1/29/2014
CONCENTRATION: 20.2mg/ml
OBSERVED MW: 70kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 110µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 76
Protocol Notes
notes unavailable
Validated AA Sequence
MVFHEITEPA IRAAAEHPRD LDIDLVDAQE TRRILDRLYG YEVSPVLWKK VAPKLSAGRV QSVATRIIVA RERDRMAFRS AAYWDILAKL DASVSXPDAA PPTFSARLTA VAGRRVATGR DFDSLGTLRK GDEVIVLDEG SATALAAGLD GTQLTVASAE EKPYARRXYP PFMTSTLQQE ASRKLRFSAE RTMSIAQRLY ENGYITYMRT DSTTLSESAI NAARTQARQL YGDEYVAPAP RQYTRKVKNA QEAHEAIRPA GETFATPDAV RRELDGPNID DFRLYELIWQ RTVASQMADA RGMTLSLRIT GMSGHQEVVF SATGRTLTFP GFLKAYVETV DELVGGEADD AERRLPHLTP GQRLDIVELT PDGHATNPPA RYTEASLVKA LEELGIGRPS TYSSIIKTIQ DRGYVHKKGS ALVPSWVAFA VTGLLEQHFG RLVDYDFTAA MEDELDEIAA GNERRTNWLN NFYFG
Validated NT Sequence
atcttactca cttaaccaaa gtagaagttg ttgagccagt tggtgcggcg ctcgttgccg gcggcgatct cgtcgagctc gtcttccatc gccgcggtga agtcgtagtc gacgagccga ccgaaatgct gctcgagcag accggttacc gcgaacgcca cccatgacgg caccagtgca ctgcccttct tgtgcacgta gccgcgatcc tggatggtct tgatgatcga cgagtaggtc gacgggcggc cgatgcccag ctcctcgagc gctttgacca gcgacgcctc ggtgtagcgg gccggcgggt tggtggcatg gccgtctggg gtcaactcga cgatgtccaa ccgttgaccc ggggtcagat ggggcagtcg ccgctcggca tcgtcagcct cgccgccgac cagctcgtcc acggtctcca cgtaggcctt gaggaagccc gggaacgtca aggtgcgtcc ggtcgcggag aacaccacct cctggtgccc cgacatgcca gtgatccgca ggctcagcgt catgccccgc gcatcggcca tctgcgaggc tacggtgcgt tgccaaatca gctcatagag ccggaaatca tcaatgttgg gaccgtcgag ttcgcgacgc accgcgtccg gggtggcaaa cgtttcaccg gcgggccgga tagcctcgtg cgcttcctgg gcgttcttca ccttgcgggt gtattggcgc ggcgccggcg cgacgtactc gtcgccgtag agctggcgcg cctgggtacg tgcggcgttg atcgccgact ccgacagcgt ggtggagtcg gtacgcatat aggtgatgta gccgttttcg tacagccgct gggcgatgct catcgtccgc tcggcggaga accgcagctt gcggctggcc tcttgctgca gcgtggaggt catgaacggc gggtacnngc gccgggcgta gggcttctcc tcggccgagg ccacggtcag ctgcgtgcca tccaggcccg cggccaacgc ggtcgcgctc ccctcgtcga gcacaatgac ttcgtcgcct ttgcgcagcg tgcccagcga gtcgaaatcg cggccagtgg ccacccgccg nccagccacg gccgtcagcc gggcgctgaa ggtgggcggc gcggcgtccg gntcggacac gctggcatcc agcttggcaa ggatgtccca gtaggccgcg ctgcggaacg ccatgcggtc gcgttcgcgc gccacgatga tgcgggtggc caccgactgc acccggcccg ccgacaactt gggggcgacc ttcttccaca gcactgggct gacttcgtag ccgtacagcc ggtccaggat gcgccgggtc tcctgcgcgt cgaccaggtc gatgtctagg tcgcgggggt gctcggcggc ggcgcggatc gccggttcgg tgatctcgtg gaagaccatc cgctttaccg gtatgcgcgg tttgagggtt tccagcagat gccaggcaat agcttcgccc tcacggtccc catccgtggc cagatacagc tcgtccacgt ctttgagcag gcccctgagc tcgctgacgg tgctccgttt ctccgggctg atgatgtaga gcggttcgaa gtcggcgtcg acgttgaccc cgagccgcgc ccacggctgc gacttgtact ttgcgggtac atccgacgcg gcccgcggca agtcacggat gtgcccccgg gaggactcga cgatgtagcc agagcccagg taggaggcca gcttgcgcgc cttggtgggc gactcgacga tgaccagtcg ccggccgctg ccattgccgc cgctgccacg gcccttcgtt ttcgggtcag cc
Expressed Protein Sequence
MAHHHHHHMA DPKTKGRGSG GNGSGRRLVI VESPTKARKL ASYLGSGYIV ESSRGHIRDL PRAASDVPAK YKSQPWARLG VNVDADFEPL YIISPEKRST VSELRGLLKD VDELYLATDG DREGEAIAWH LLETLKPRIP VKRMVFHEIT EPAIRAAAEH PRDLDIDLVD AQETRRILDR LYGYEVSPVL WKKVAPKLSA GRVQSVATRI IVARERDRMA FRSAAYWDIL AKLDASVSDP DAAPPTFSAR LTAVAGRRVA TGRDFDSLGT LRKGDEVIVL DEGSATALAA GLDGTQLTVA SAEEKPYARR PYPPFMTSTL QQEASRKLRF SAERTMSIAQ RLYENGYITY MRTDSTTLSE SAINAARTQA RQLYGDEYVA PAPRQYTRKV KNAQEAHEAI RPAGETFATP DAVRRELDGP NIDDFRLYEL IWQRTVASQM ADARGMTLSL RITGMSGHQE VVFSATGRTL TFPGFLKAYV ETVDELVGGE ADDAERRLPH LTPGQRLDIV ELTPDGHATN PPARYTEASL VKALEELGIG RPSTYSSIIK TIQDRGYVHK KGSALVPSWV AFAVTGLLEQ HFGRLVDYDF TAAMEDELDE IAAGNERRTN WLNNFYFG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTTGGCTG ACCCGAAAAC GAAGGGCCGT GGCAGCGGCG GCAATGGCAG CGGCCGGCGA CTGGTCATCG TCGAGTCGCC CACCAAGGCG CGCAAGCTGG CCTCCTACCT GGGCTCTGGC TACATCGTCG AGTCCTCCCG GGGGCACATC CGTGACTTGC CGCGGGCCGC GTCGGATGTA CCCGCAAAGT ACAAGTCGCA GCCGTGGGCG CGGCTCGGGG TCAACGTCGA CGCCGACTTC GAACCGCTCT ACATCATCAG CCCGGAGAAA CGGAGCACCG TCAGCGAGCT CAGGGGCCTG CTCAAAGACG TGGACGAGCT GTATCTGGCC ACGGATGGGG ACCGTGAGGG CGAAGCTATT GCCTGGCATC TGCTGGAAAC CCTCAAACCG CGCATACCGG TAAAGCGGAT GGTCTTCCAC GAGATCACCG AACCGGCGAT CCGCGCCGCC GCCGAGCACC CCCGCGACCT AGACATCGAC CTGGTCGACG CGCAGGAGAC CCGGCGCATC CTGGACCGGC TGTACGGCTA CGAAGTCAGC CCAGTGCTGT GGAAGAAGGT CGCCCCCAAG TTGTCGGCGG GCCGGGTGCA GTCGGTGGCC ACCCGCATCA TCGTGGCGCG CGAACGCGAC CGCATGGCGT TCCGCAGCGC GGCCTACTGG GACATCCTTG CCAAGCTGGA TGCCAGCGTG TCCGACCCGG ACGCCGCGCC GCCCACCTTC AGCGCCCGGC TGACGGCCGT GGCTGGCCGG CGGGTGGCCA CTGGCCGCGA TTTCGACTCG CTGGGCACGC TGCGCAAAGG CGACGAAGTC ATTGTGCTCG ACGAGGGGAG CGCGACCGCG TTGGCCGCGG GCCTGGATGG CACGCAGCTG ACCGTGGCCT CGGCCGAGGA GAAGCCCTAC GCCCGGCGCC CGTACCCGCC GTTCATGACC TCCACGCTGC AGCAAGAGGC CAGCCGCAAG CTGCGGTTCT CCGCCGAGCG GACGATGAGC ATCGCCCAGC GGCTGTACGA AAACGGCTAC ATCACCTATA TGCGTACCGA CTCCACCACG CTGTCGGAGT CGGCGATCAA CGCCGCACGT ACCCAGGCGC GCCAGCTCTA CGGCGACGAG TACGTCGCGC CGGCGCCGCG CCAATACACC CGCAAGGTGA AGAACGCCCA GGAAGCGCAC GAGGCTATCC GGCCCGCCGG TGAAACGTTT GCCACCCCGG ACGCGGTGCG TCGCGAACTC GACGGTCCCA ACATTGATGA TTTCCGGCTC TATGAGCTGA TTTGGCAACG CACCGTAGCC TCGCAGATGG CCGATGCGCG GGGCATGACG CTGAGCCTGC GGATCACTGG CATGTCGGGG CACCAGGAGG TGGTGTTCTC CGCGACCGGA CGCACCTTGA CGTTCCCGGG CTTCCTCAAG GCCTACGTGG AGACCGTGGA CGAGCTGGTC GGCGGCGAGG CTGACGATGC CGAGCGGCGA CTGCCCCATC TGACCCCGGG TCAACGGTTG GACATCGTCG AGTTGACCCC AGACGGCCAT GCCACCAACC CGCCGGCCCG CTACACCGAG GCGTCGCTGG TCAAAGCGCT CGAGGAGCTG GGCATCGGCC GCCCGTCGAC CTACTCGTCG ATCATCAAGA CCATCCAGGA TCGCGGCTAC GTGCACAAGA AGGGCAGTGC ACTGGTGCCG TCATGGGTGG CGTTCGCGGT AACCGGTCTG CTCGAGCAGC ATTTCGGTCG GCTCGTCGAC TACGACTTCA CCGCGGCGAT GGAAGACGAG CTCGACGAGA TCGCCGCCGG CAACGAGCGC CGCACCAACT GGCTCAACAA CTTCTACTTT GGTTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT
Details for MytuD.18062.a.B31.PW37506
PURIFICATION DATe: 6/11/2014
CONCENTRATION: 28.2mg/ml
OBSERVED MW: 73kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 89
PERCENT COVERAGE: 66
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML ADPKTKGRGS GGNGSGRRLV IVESPTKARK LASYLGSGYI VESSRGHIRD LPRAASDVPA KYKSQPWARL GVNVDADFEP LYIISPEKRS TVSELRGLLK DVDELYLATD GDREGEAIAW HLLETLKPRI PVKRMVFHEI TEPAIRAAAE HPRDLDIDLV DAQETRRILD RLYGYEVSPV LWKKVAPKLS AGRVQSVATR IIVARERDRM AFRSAAYWDI LAKLDASVSD PDAAPPTFSA RLTAVAGRRV ATGRDFDSLG TLRKGDEVIV LDEGSATALA AGLDGTQLTV ASAEEKPYAR RPYPPFMTST LQQEASRKLR FSAERTMSIA QRLYENGYXT FMRTDSXRCR SRRSTPHGTQ AXXLXRAXXY VXPRPXXPXX XXXXXXEXXP XRVKXXXXP
Validated NT Sequence
gnnnntnnnn nnnnnnttnt tgtttaactt taagaaggag atataccatg gctcaccacc accaccacca tatgttggct gacccgaaaa cgaagggccg tggcagcggc ggcaatggca gcggccggcg actggtcatc gtcgagtcgc ccaccaaggc gcgcaagctg gcctcctacc tgggctctgg ctacatcgtc gagtcctccc gggggcacat ccgtgacttg ccgcgggccg cgtcggatgt acccgcaaag tacaagtcgc agccgtgggc gcggctcggg gtcaacgtcg acgccgactt cgaaccgctc tacatcatca gcccggagaa acggagcacc gtcagcgagc tcaggggcct gctcaaagac gtggacgagc tgtatctggc cacggatggg gaccgtgagg gcgaagctat tgcctggcat ctgctggaaa ccctcaaacc gcgcataccg gtaaagcgga tggtcttcca cgagatcacc gaaccggcga tccgcgccgc cgccgagcac ccccgcgacc tagacatcga cctggtcgac gcgcaggaga cccggcgcat cctggaccgg ctgtacggct acgaagtcag cccagtgctg tggaagaagg tcgcccccaa gttgtcggcg ggccgggtgc agtcggtggc cacccgcatc atcgtggcgc gcgaacgcga ccgcatggcg ttccgcagcg cggcctactg ggacatcctt gccaagctgg atgccagcgt gtccgacccg gacgccgcgc cgcccacctt cagcgcccgg ctgacggccg tggctggccg gcgggtggcc actggccgcg atttcgactc gctgggcacg ctgcgcaaag gcgacgaagt cattgtgctc gacgagggga gcgcgaccgc gttggccgcg ggcctggatg gcacgcagct gaccgtggcc tcggccgagg agaagcccta cgcccggcgc ccgtacccgc cgttcatgac ctccacgctg cagcaagagg ccagccgcaa gctgcggttc tccgccgagc ggacgatgag catcgcccag cggctgtacg aaaacggcta cntcaccttt atgcgtaccg actccncacg ctgtcggagt cggcgatcaa cgccgcacgg tacccaggcg ngccanctct tncgggcnnc nangtacgtc nnnccncgnc cnntnnnncc nncnnnnnna nnncnnnnnn nnnangagcn tnnncccnnc cgggtgaaan nnnnncnnnn ccccc
Expressed Protein Sequence
MAHHHHHHLA DPKTKGRGSG GNGSGRRLVI VESPTKARKL ASYLGSGYIV ESSRGHIRDL PRAASDVPAK YKSQPWARLG VNVDADFEPL YIISPEKRST VSELRGLLKD VDELYLATDG DREGEAIAWH LLETLKPRIP VKRMVFHEIT EPAIRAAAEH PRDLDIDLVD AQETRRILDR LYGYEVSPVL WKKVAPKLSA GRVQSVATRI IVARERDRMA FRSAAYWDIL AKLDASVSDP DAAPPTFSAR LTAVAGRRVA TGRDFDSLGT LRKGDEVIVL DEGSATALAA GLDGTQLTVA SAEEKPYARR PYPPFMTSTL QQEASRKLRF SAERTMSIAQ RLYENGYITF MRTDSTTLSE SAINAARTQA RQLYGDEYVA PAPRQYTRKV KNAQEAHEAI RPAGETFATP DAVRRELDGP NIDDFRLYEL IWQRTVASQM ADARGMTLSL RITGMSGHQE VVFSATGRTL TFPGFLKAYV ETVDELVGGE ADDAERRLPH LTPGQRLDIV ELTPDGHATN PPARYTEASL VKALEELGIG RPSTYSSIIK TIQDRGYVHK KGSALVPSWV AFAVTGLLEQ HFGRLVDYDF TAAMEDELDE IAAGNERRTN WLNNFYFG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTTGGCTG ACCCGAAAAC GAAGGGCCGT GGCAGCGGCG GCAATGGCAG CGGCCGGCGA CTGGTCATCG TCGAGTCGCC CACCAAGGCG CGCAAGCTGG CCTCCTACCT GGGCTCTGGC TACATCGTCG AGTCCTCCCG GGGGCACATC CGTGACTTGC CGCGGGCCGC GTCGGATGTA CCCGCAAAGT ACAAGTCGCA GCCGTGGGCG CGGCTCGGGG TCAACGTCGA CGCCGACTTC GAACCGCTCT ACATCATCAG CCCGGAGAAA CGGAGCACCG TCAGCGAGCT CAGGGGCCTG CTCAAAGACG TGGACGAGCT GTATCTGGCC ACGGATGGGG ACCGTGAGGG CGAAGCTATT GCCTGGCATC TGCTGGAAAC CCTCAAACCG CGCATACCGG TAAAGCGGAT GGTCTTCCAC GAGATCACCG AACCGGCGAT CCGCGCCGCC GCCGAGCACC CCCGCGACCT AGACATCGAC CTGGTCGACG CGCAGGAGAC CCGGCGCATC CTGGACCGGC TGTACGGCTA CGAAGTCAGC CCAGTGCTGT GGAAGAAGGT CGCCCCCAAG TTGTCGGCGG GCCGGGTGCA GTCGGTGGCC ACCCGCATCA TCGTGGCGCG CGAACGCGAC CGCATGGCGT TCCGCAGCGC GGCCTACTGG GACATCCTTG CCAAGCTGGA TGCCAGCGTG TCCGACCCGG ACGCCGCGCC GCCCACCTTC AGCGCCCGGC TGACGGCCGT GGCTGGCCGG CGGGTGGCCA CTGGCCGCGA TTTCGACTCG CTGGGCACGC TGCGCAAAGG CGACGAAGTC ATTGTGCTCG ACGAGGGGAG CGCGACCGCG TTGGCCGCGG GCCTGGATGG CACGCAGCTG ACCGTGGCCT CGGCCGAGGA GAAGCCCTAC GCCCGGCGCC CGTACCCGCC GTTCATGACC TCCACGCTGC AGCAAGAGGC CAGCCGCAAG CTGCGGTTCT CCGCCGAGCG GACGATGAGC ATCGCCCAGC GGCTGTACGA AAACGGCTAC ATCACCTTTA TGCGTACCGA CTCCACCACG CTGTCGGAGT CGGCGATCAA CGCCGCACGT ACCCAGGCGC GCCAGCTCTA CGGCGACGAG TACGTCGCGC CGGCGCCGCG CCAATACACC CGCAAGGTGA AGAACGCCCA GGAAGCGCAC GAGGCTATCC GGCCCGCCGG TGAAACGTTT GCCACCCCGG ACGCGGTGCG TCGCGAACTC GACGGTCCCA ACATTGATGA TTTCCGGCTC TATGAGCTGA TTTGGCAACG CACCGTAGCC TCGCAGATGG CCGATGCGCG GGGCATGACG CTGAGCCTGC GGATCACTGG CATGTCGGGG CACCAGGAGG TGGTGTTCTC CGCGACCGGA CGCACCTTGA CGTTCCCGGG CTTCCTCAAG GCCTACGTGG AGACCGTGGA CGAGCTGGTC GGCGGCGAGG CTGACGATGC CGAGCGGCGA CTGCCCCATC TGACCCCGGG TCAACGGTTG GACATCGTCG AGTTGACCCC AGACGGCCAT GCCACCAACC CGCCGGCCCG CTACACCGAG GCGTCGCTGG TCAAAGCGCT CGAGGAGCTG GGCATCGGCC GCCCGTCGAC CTACTCGTCG ATCATCAAGA CCATCCAGGA TCGCGGCTAC GTGCACAAGA AGGGCAGTGC ACTGGTGCCG TCATGGGTGG CGTTCGCGGT AACCGGTCTG CTCGAGCAGC ATTTCGGTCG GCTCGTCGAC TACGACTTCA CCGCGGCGAT GGAAGACGAG CTCGACGAGA TCGCCGCCGG CAACGAGCGC CGCACCAACT GGCTCAACAA CTTCTACTTT GGTTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT