MytuD.17943.a

Leucine--tRNA ligase

CENTER ID: MytuD.17943.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MytuD.17943.a.B1.GE37073 full length 1 969
MytuD.17943.a.A8.GE38389 Edit domain 4 275 560
MytuD.17943.a.A7.GE38388 Edit domain 3 394 530
MytuD.17943.a.A5.GE38386 Edit domain 1 275 530
MytuD.17943.a.A4.GE38385 N and C term truncation of full length 34 944
MytuD.17943.a.A3.GE38384 C term truncation of full length 1 944
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MytuD.17943.a.A5.PW37567 Edit domain 1 275 530
MytuD.17943.a.A8.PW37568 Edit domain 4 275 560

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.46
RefSeq: NP_214555.1
RefSeq: YP_006513355.1
TubercuList: Rv0041
MTB Network Portal: Rv0041
UniProt: P9WFV1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTESPTAGPG GVPRADDADS DVPRYRYTAE LAARLERTWQ ENWARLGTFN VPNPVGSLAP PDGAAVPDDK LFVQDMFPYP SGEGLHVGHP LGYIATDVYA RYFRMVGRNV LHALGFDAFG LPAEQYAVQT GTHPRTRTEA NVVNFRRQLG RLGFGHDSRR SFSTTDVDFY RWTQWIFLQI YNAWFDTTAN KARPISELVA EFESGARCLD GGRDWAKLTA GERADVIDEY RLVYRADSLV NWCPGLGTVL ANEEVTADGR SDRGNFPVFR KRLRQWMMRI TAYADRLLDD LDVLDWPEQV KTMQRNWIGR STGAVALFSA RAASDDGFEV DIEVFTTRPD TLFGATYLVL APEHDLVDEL VAASWPAGVN PLWTYGGGTP GEAIAAYRRA IAAKSDLERQ ESREKTGVFL GSYAINPANG EPVPIFIADY VLAGYGTGAI MAVPGHDQRD WDFARAFGLP IVEVIAGGNI SESAYTGDGI LVNSDYLNGM SVPAAKRAIV DRLESAGRGR ARIEFKLRDW LFARQRYWGE PFPIVYDSDG RPHALDEAAL PVELPDVPDY SPVLFDPDDA DSEPSPPLAK ATEWVHVDLD LGDGLKPYSR DTNVMPQWAG SSWYELRYTD PHNSERFCAK ENEAYWMGPR PAEHGPDDPG GVDLYVGGAE HAVLHLLYSR FWHKVLYDLG HVSSREPYRR LVNQGYIQAY AYTDARGSYV PAEQVIERGD RFVYPGPDGE VEVFQEFGKI GKSLKNSVSP DEICDAYGAD TLRVYEMSMG PLEASRPWAT KDVVGAYRFL QRVWRLVVDE HTGETRVADG VELDIDTLRA LHRTIVGVSE DFAALRNNTA TAKLIEYTNH LTKKHRDAVP RAAVEPLVQM LAPLAPHIAE ELWLRLGNTT SLAHGPFPKA DAAYLVDETV EYPVQVNGKV RGRVVVAADT DEETLKAAVL TDEKVQAFLA GATPRKVIVV AGRLVNLVI
NT Sequence
gtgaccgaat cgccaaccgc tgggcctggc ggcgtgcccc gtgccgacga cgcggactcc gacgtgccac ggtaccgcta taccgccgag ctcgcggcta ggctggaacg gacctggcag gaaaactggg cccggctagg gacgttcaac gtgcccaacc cggtcggctc gctggcccca ccggatggtg ccgcggtgcc tgacgacaag ctcttcgtgc aggacatgtt cccctacccc tcgggtgagg gactccacgt tggtcatccc ctcggctaca tcgcgaccga cgtctatgcc cgctatttcc ggatggtggg ccgtaatgtg ctgcatgcgc tagggttcga cgcgttcggg ctgcccgccg agcaatacgc ggtacaaacc ggcacccatc cgcgtacccg gaccgaagcc aacgtcgtca actttcgccg ccagttgggc cggctgggct tcggccacga cagccgacga agcttctcga ccaccgatgt cgacttctac aggtggactc agtggatctt cctacagata tacaacgcgt ggttcgacac cacagccaac aaggcgcgcc cgatatcaga gctggtcgcc gaattcgagt ccggtgcaag gtgtctcgat ggcggccggg attgggccaa gttgaccgcg ggggagcgag ccgatgtgat cgacgagtac cggctggtct atcgggcgga ttcgctggtg aactggtgcc cggggctagg tacggtgctt gccaacgaag aggtgaccgc cgacggccgc agcgaccggg gcaattttcc ggtgttccgg aagcggttgc ggcaatggat gatgcggatc accgcctatg ccgaccggct gctcgacgac ctggatgtgc tggattggcc tgagcaggtc aagaccatgc agcgcaactg gatcgggcgt tcgacgggtg cggtggcgct gttctcggcg agagcggcca gcgatgacgg gttcgaagtc gacatcgagg tgttcaccac gcggcccgac accttgttcg gcgccacgta tctggtgctg gctcccgagc acgacttggt cgacgagttg gtcgccgcgt cctggccggc tggggtcaac cccttgtgga catacggcgg cggcacacct ggtgaggcca tcgccgccta ccggcgtgcg atcgccgcca aatcagacct cgagcgccag gagagcaggg aaaagaccgg cgtcttcttg ggcagctacg ccatcaaccc ggccaacggt gagccggtgc cgatcttcat cgccgactac gtgctggccg ggtacggtac cggggcaatc atggcggtgc cgggtcatga ccagcgggac tgggacttcg ctcgggcatt tggtctaccg atcgtggaag taattgccgg cggcaatatt tcggaatccg cgtatacagg cgatggcatc ctggtcaact cggattacct caatggaatg agcgtgccag cagcaaagcg ggccatcgtc gaccggttgg agtccgcggg ccgcggccgg gctcgaatcg aattcaaatt gcgcgactgg ctttttgcgc ggcagcggta ttggggtgaa ccattcccga tcgtctatga cagcgacggg cgtccgcatg cgctcgacga agctgcactg cccgtcgagc tgcctgatgt cccggactac tcgccggttt tgttcgaccc cgacgatgcg gacagcgagc cttcgccccc actggccaag gcgactgagt gggtacacgt cgacctggac ctcggtgatg gcctgaagcc ctacagccgc gacaccaacg tgatgccgca gtgggcgggc agctcctggt atgaactgcg ctacaccgat ccgcacaact cagaacggtt ctgcgccaag gaaaacgagg cctattggat gggaccgcgg ccggctgagc acggcccgga cgaccccggt ggcgtcgact tgtacgtcgg cggtgctgaa cacgcggttt tgcacctgct gtattccagg ttctggcaca aggtcttgta cgacctgggt cacgtcagct ctcgcgagcc ttaccgcagg ctggtcaatc agggctatat tcaagcttac gcttacaccg atgcgcgcgg atcctatgtc cctgccgagc aggtgatcga acgcggtgac agatttgtct atcctggacc tgacggtgag gtcgaagttt tccaggaatt cggcaaaatc ggtaagagcc tgaagaattc ggtatcgccg gacgaaatct gcgacgcata cggggcagat acgcttcggg tttacgagat gtcgatgggg ccgctggagg cttcacgtcc atgggccaca aaggatgttg tcggcgcgta ccgttttctg cagcgggtgt ggcgcttggt cgtcgacgag cacaccggcg aaactcgggt ggctgacggc gtggaactcg acatcgatac gctacgggcg ttgcaccgca ccatcgtcgg cgtgtcagaa gactttgcgg cacttcgcaa taacaccgca acggctaagt tgatcgaata cacgaaccac ctcaccaaga agcatcgtga tgcggtgcct cgggccgccg tggagccgct tgtacaaatg ctggctccgc tggccccaca tattgccgag gagctgtggc tgcgactggg caacaccacc tcgttggcac acggcccgtt cccgaaggcc gatgccgcct acctcgtcga cgagacggtc gagtatccgg tgcaggtgaa cggcaaggta cgtggccggg tggtggtggc cgccgacacc gacgaggaaa cgctgaaagc cgccgttctg accgacgaaa aggtccaggc attcttggct ggtgccaccc cgcgcaaggt tatcgtggtc gccggccggc tggtcaatct cgtcatctag
Details for MytuD.17943.a.B1.GE37073
HARVESTED ON: 6/12/2013
SEQUENCED ON: 6/11/2013
EXPECTED MW: 109kDa
OBSERVED MW: 109kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 82
PERCENT COVERAGE: 39
Validated AA Sequence
MAHHHHHHMV TESPTAGPGG VPRADDADSD VPRYRYTAEL AARLERTWQE NWARLGTFNV PNPVGSLAPP DGAAVPDDKL FVQDMFPYPS GEGLHVGHPL GYIATDVYAR YFRMVGRNVL HALGFDAFGL PAEQYAVQTG THPRTRTEAN VVNFRRQLGR LGFGHDSRRS FSTTDVDFYR WTQWIFLQIY NAWFDTTANK ARPISXLVAE FESGARCLDG GRDWAKLTAG ERXDVIDXYR LVYRADSLVN WCPGLGTVLA NEXVXADGRX DRGNFPVFXK XLRXWMMRIX XYADRLLDDL DVLDXPEQVK TMXRNWIGRS XXAVXLFSAX AXSXXGFXVX XXVXXXXXXX XXXXXXXXXX EXXXXXXXXX XXXXXXXXXX XXXXXXXXXX
Validated NT Sequence
ttgtttaact ttaagaagga gatataccat ggctcaccac caccaccacc atatggtgac cgaatcgcca accgctgggc ctggcggcgt gccccgtgcc gacgacgcgg actccgacgt gccacggtac cgctataccg ccgagctcgc ggctaggctg gaacggacct ggcaggaaaa ctgggcccgg ctagggacgt tcaacgtgcc caacccggtc ggctcgctgg ccccaccgga tggtgccgcg gtgcctgacg acaagctctt cgtgcaggac atgttcccct acccctcggg tgagggactc cacgttggtc atcccctcgg ctacatcgcg accgacgtct atgcccgcta tttccggatg gtgggccgta atgtgctgca tgcgctaggg ttcgacgcgt tcgggctgcc cgccgagcaa tacgcggtac aaaccggcac ccatccgcgt acccggaccg aagccaacgt cgtcaacttt cgccgccagt tgggccggct gggcttcggc cacgacagcc gacgaagctt ctcgaccacc gatgtcgact tctacaggtg gactcagtgg atcttcctac agatatacaa cgcgtggttc gacaccacag ccaacaaggc gcgcccgata tcanagctgg tcgccgaatt cgagtccggt gcaaggtgtc tcgatggcgg ccgggattgg gccaagttga ccgcggggga gcgagncgat gtgatcgacn agtaccggct ggtctatcgg gcggattcnc tggtgaactg gtgcccgggg ctaggtacgg tgcttgccaa cgaanaggtg ancgccgacg gccgcnncga ccggggcaat tttccggtgt tccngaagcn gttgcggcan tggatgatgc gnatcnnnnn ctatgccgac cggctgctcg acgacctgga tgtgctggat ngncctgagc aggtcaagac catgcnncgc aactggatcg ggcgttcnan ggnngcggtn gnnctgttct cggcnanagc gnccagcnat gangggttcn aagtcnannn cnaggtgntc nncnnnncng nccnnnncnn nnngnnnncc ncnnnncngn gctgnnncga gcnnnnctnn gnacnnnnnn nnnnnnncnn nnnnnnnngn nnnancnnnn ganntnngnn gnnnnnnnng nnnnnannnn nnnnnnnnnc
Expected Protein Sequence
MAHHHHHHMT ESPTAGPGGV PRADDADSDV PRYRYTAELA ARLERTWQEN WARLGTFNVP NPVGSLAPPD GAAVPDDKLF VQDMFPYPSG EGLHVGHPLG YIATDVYARY FRMVGRNVLH ALGFDAFGLP AEQYAVQTGT HPRTRTEANV VNFRRQLGRL GFGHDSRRSF STTDVDFYRW TQWIFLQIYN AWFDTTANKA RPISELVAEF ESGARCLDGG RDWAKLTAGE RADVIDEYRL VYRADSLVNW CPGLGTVLAN EEVTADGRSD RGNFPVFRKR LRQWMMRITA YADRLLDDLD VLDWPEQVKT MQRNWIGRST GAVALFSARA ASDDGFEVDI EVFTTRPDTL FGATYLVLAP EHDLVDELVA ASWPAGVNPL WTYGGGTPGE AIAAYRRAIA AKSDLERQES REKTGVFLGS YAINPANGEP VPIFIADYVL AGYGTGAIMA VPGHDQRDWD FARAFGLPIV EVIAGGNISE SAYTGDGILV NSDYLNGMSV PAAKRAIVDR LESAGRGRAR IEFKLRDWLF ARQRYWGEPF PIVYDSDGRP HALDEAALPV ELPDVPDYSP VLFDPDDADS EPSPPLAKAT EWVHVDLDLG DGLKPYSRDT NVMPQWAGSS WYELRYTDPH NSERFCAKEN EAYWMGPRPA EHGPDDPGGV DLYVGGAEHA VLHLLYSRFW HKVLYDLGHV SSREPYRRLV NQGYIQAYAY TDARGSYVPA EQVIERGDRF VYPGPDGEVE VFQEFGKIGK SLKNSVSPDE ICDAYGADTL RVYEMSMGPL EASRPWATKD VVGAYRFLQR VWRLVVDEHT GETRVADGVE LDIDTLRALH RTIVGVSEDF AALRNNTATA KLIEYTNHLT KKHRDAVPRA AVEPLVQMLA PLAPHIAEEL WLRLGNTTSL AHGPFPKADA AYLVDETVEY PVQVNGKVRG RVVVAADTDE ETLKAAVLTD EKVQAFLAGA TPRKVIVVAG RLVNLVI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgaccg aatcgccaac cgctgggcct ggcggcgtgc cccgtgccga cgacgcggac tccgacgtgc cacggtaccg ctataccgcc gagctcgcgg ctaggctgga acggacctgg caggaaaact gggcccggct agggacgttc aacgtgccca acccggtcgg ctcgctggcc ccaccggatg gtgccgcggt gcctgacgac aagctcttcg tgcaggacat gttcccctac ccctcgggtg agggactcca cgttggtcat cccctcggct acatcgcgac cgacgtctat gcccgctatt tccggatggt gggccgtaat gtgctgcatg cgctagggtt cgacgcgttc gggctgcccg ccgagcaata cgcggtacaa accggcaccc atccgcgtac ccggaccgaa gccaacgtcg tcaactttcg ccgccagttg ggccggctgg gcttcggcca cgacagccga cgaagcttct cgaccaccga tgtcgacttc tacaggtgga ctcagtggat cttcctacag atatacaacg cgtggttcga caccacagcc aacaaggcgc gcccgatatc agagctggtc gccgaattcg agtccggtgc aaggtgtctc gatggcggcc gggattgggc caagttgacc gcgggggagc gagccgatgt gatcgacgag taccggctgg tctatcgggc ggattcgctg gtgaactggt gcccggggct aggtacggtg cttgccaacg aagaggtgac cgccgacggc cgcagcgacc ggggcaattt tccggtgttc cggaagcggt tgcggcaatg gatgatgcgg atcaccgcct atgccgaccg gctgctcgac gacctggatg tgctggattg gcctgagcag gtcaagacca tgcagcgcaa ctggatcggg cgttcgacgg gtgcggtggc gctgttctcg gcgagagcgg ccagcgatga cgggttcgaa gtcgacatcg aggtgttcac cacgcggccc gacaccttgt tcggcgccac gtatctggtg ctggctcccg agcacgactt ggtcgacgag ttggtcgccg cgtcctggcc ggctggggtc aaccccttgt ggacatacgg cggcggcaca cctggtgagg ccatcgccgc ctaccggcgt gcgatcgccg ccaaatcaga cctcgagcgc caggagagca gggaaaagac cggcgtcttc ttgggcagct acgccatcaa cccggccaac ggtgagccgg tgccgatctt catcgccgac tacgtgctgg ccgggtacgg taccggggca atcatggcgg tgccgggtca tgaccagcgg gactgggact tcgctcgggc atttggtcta ccgatcgtgg aagtaattgc cggcggcaat atttcggaat ccgcgtatac aggcgatggc atcctggtca actcggatta cctcaatgga atgagcgtgc cagcagcaaa gcgggccatc gtcgaccggt tggagtccgc gggccgcggc cgggctcgaa tcgaattcaa attgcgcgac tggctttttg cgcggcagcg gtattggggt gaaccattcc cgatcgtcta tgacagcgac gggcgtccgc atgcgctcga cgaagctgca ctgcccgtcg agctgcctga tgtcccggac tactcgccgg ttttgttcga ccccgacgat gcggacagcg agccttcgcc cccactggcc aaggcgactg agtgggtaca cgtcgacctg gacctcggtg atggcctgaa gccctacagc cgcgacacca acgtgatgcc gcagtgggcg ggcagctcct ggtatgaact gcgctacacc gatccgcaca actcagaacg gttctgcgcc aaggaaaacg aggcctattg gatgggaccg cggccggctg agcacggccc ggacgacccc ggtggcgtcg acttgtacgt cggcggtgct gaacacgcgg ttttgcacct gctgtattcc aggttctggc acaaggtctt gtacgacctg ggtcacgtca gctctcgcga gccttaccgc aggctggtca atcagggcta tattcaagct tacgcttaca ccgatgcgcg cggatcctat gtccctgccg agcaggtgat cgaacgcggt gacagatttg tctatcctgg acctgacggt gaggtcgaag ttttccagga attcggcaaa atcggtaaga gcctgaagaa ttcggtatcg ccggacgaaa tctgcgacgc atacggggca gatacgcttc gggtttacga gatgtcgatg gggccgctgg aggcttcacg tccatgggcc acaaaggatg ttgtcggcgc gtaccgtttt ctgcagcggg tgtggcgctt ggtcgtcgac gagcacaccg gcgaaactcg ggtggctgac ggcgtggaac tcgacatcga tacgctacgg gcgttgcacc gcaccatcgt cggcgtgtca gaagactttg cggcacttcg caataacacc gcaacggcta agttgatcga atacacgaac cacctcacca agaagcatcg tgatgcggtg cctcgggccg ccgtggagcc gcttgtacaa atgctggctc cgctggcccc acatattgcc gaggagctgt ggctgcgact gggcaacacc acctcgttgg cacacggccc gttcccgaag gccgatgccg cctacctcgt cgacgagacg gtcgagtatc cggtgcaggt gaacggcaag gtacgtggcc gggtggtggt ggccgccgac accgacgagg aaacgctgaa agccgccgtt ctgaccgacg aaaaggtcca ggcattcttg gctggtgcca ccccgcgcaa ggttatcgtg gtcgccggcc ggctggtcaa tctcgtcatc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for MytuD.17943.a.A8.GE38389
HARVESTED ON: 8/8/2014
SEQUENCED ON: 8/13/2014
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMQWMMRITA YADRLLDDLD VLDWPEQVKT MQRNWIGRST GAVALFSARA ASDDGFEVDI EVFTTRPDTL FGATYLVLAP EHDLVDELVA ASWPAGVNPL WTYGGGTPGE AIAAYRRAIA AKSDLERQES REKTGVFLGS YAINPANGEP VPIFIADYVL AGYGTGAIMA VPGHDQRDWD FARAFGLPIV EVIAGGNISE SAYTGDGILV NSDYLNGMSV PAAKRAIVDR LESAGRGRAR IEFKLRDWLF ARQRYWGEPF PIVYDSDGRP HALDEAALPV ELPDL
Validated NT Sequence
cnnnannntn attttgttta actttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgcaa tggatgatgc ggatcaccgc ctatgccgac cggctgctcg acgacctgga tgtgctggat tggcctgagc aggtcaagac catgcagcgc aactggatcg ggcgttcgac gggtgcggtg gcgctgttct cggcgagagc ggccagcgat gacgggttcg aagtcgacat cgaggtgttc accacgcggc ccgacacctt gttcggcgcc acgtatctgg tgctggctcc cgagcacgac ttggtcgacg agttggtcgc cgcgtcctgg ccggctgggg tcaacccctt gtggacatac ggcggcggca cacctggtga ggccatcgcc gcctaccggc gtgcgatcgc cgccaaatca gacctcgagc gccaggagag cagggaaaag accggcgtct tcttgggcag ctacgccatc aacccggcca acggtgagcc ggtgccgatc ttcatcgccg actacgtgct ggccgggtac ggtaccgggg caatcatggc ggtgccgggt catgaccagc gggactggga cttcgctcgg gcatttggtc taccgatcgt ggaagtaatt gccggcggca atatttcgga atccgcgtat acaggcgatg gcatcctggt caactcggat tacctcaatg gaatgagcgt gccagcagca aagcgggcca tcgtcgaccg gttggagtcc gcgggccgcg gccgggctcg aatcgaattc aaattgcgcg actggctttt tgcgcggcag cggtattggg gtgaaccatt cccgatcgtc tatgacagcg acgggcgtcc gcatgcgctc gacgaagctg cactgcccgt cgagctgcct gatttataaa cagcacgaac aagttctgca gccnagcttc tcgagntccn ctgctaacaa agcccgaaan gnnctgagtt gctgctgcac gctgagcata ctagcatanc cctnnnnncn acnnnntgnn ngnggaaann nnagaagnnn nannnantnn nnnntngnnc nncnggngnn nnngantnnn ttnnnncncn nnng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SQWMMRITAY ADRLLDDLDV LDWPEQVKTM QRNWIGRSTG AVALFSARAA SDDGFEVDIE VFTTRPDTLF GATYLVLAPE HDLVDELVAA SWPAGVNPLW TYGGGTPGEA IAAYRRAIAA KSDLERQESR EKTGVFLGSY AINPANGEPV PIFIADYVLA GYGTGAIMAV PGHDQRDWDF ARAFGLPIVE VIAGGNISES AYTGDGILVN SDYLNGMSVP AAKRAIVDRL ESAGRGRARI EFKLRDWLFA RQRYWGEPFP IVYDSDGRPH ALDEAALPVE LPDVPDY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgca atggatgatg cggatcaccg cctatgccga ccggctgctc gacgacctgg atgtgctgga ttggcctgag caggtcaaga ccatgcagcg caactggatc gggcgttcga cgggtgcggt ggcgctgttc tcggcgagag cggccagcga tgacgggttc gaagtcgaca tcgaggtgtt caccacgcgg cccgacacct tgttcggcgc cacgtatctg gtgctggctc ccgagcacga cttggtcgac gagttggtcg ccgcgtcctg gccggctggg gtcaacccct tgtggacata cggcggcggc acacctggtg aggccatcgc cgcctaccgg cgtgcgatcg ccgccaaatc agacctcgag cgccaggaga gcagggaaaa gaccggcgtc ttcttgggca gctacgccat caacccggcc aacggtgagc cggtgccgat cttcatcgcc gactacgtgc tggccgggta cggtaccggg gcaatcatgg cggtgccggg tcatgaccag cgggactggg acttcgctcg ggcatttggt ctaccgatcg tggaagtaat tgccggcggc aatatttcgg aatccgcgta tacaggcgat ggcatcctgg tcaactcgga ttacctcaat ggaatgagcg tgccagcagc aaagcgggcc atcgtcgacc ggttggagtc cgcgggccgc ggccgggctc gaatcgaatt caaattgcgc gactggcttt ttgcgcggca gcggtattgg ggtgaaccat tcccgatcgt ctatgacagc gacgggcgtc cgcatgcgct cgacgaagct gcactgcccg tcgagctgcc tgatgtcccg gactactaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for MytuD.17943.a.A7.GE38388
HARVESTED ON: 8/8/2014
SEQUENCED ON: 8/13/2014
EXPECTED MW: 17kDa
OBSERVED MW: 17kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKSDLERQE SREKTGVFLG SYAINPANGE PVPIFIADYV LAGYGTGAIM AVPGHDQRDW DFARAFGLPI VEVIAGGNIS ESAYTGDGIL VNSDYLNGMS VPAAKRAIVD RLESAGRGRA RIEFKLRDWL FARQRYWGE
Validated NT Sequence
tcnnnatatt ttgtttaact ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgaaatca gacctcgagc gccaggagag cagggaaaag accggcgtct tcttgggcag ctacgccatc aacccggcca acggtgagcc ggtgccgatc ttcatcgccg actacgtgct ggccgggtac ggtaccgggg caatcatggc ggtgccgggt catgaccagc gggactggga cttcgctcgg gcatttggtc taccgatcgt ggaagtaatt gccggcggca atatttcgga atccgcgtat acaggcgatg gcatcctggt caactcggat tacctcaatg gaatgagcgt gccagcagca aagcgggcca tcgtcgaccg gttggagtcc gcgggccgcg gccgggctcg aatcgaattc aaattgcgcg actggctttt tgcgcggcag cggtattggg gtgaataata aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gnnnngaggg gtanngcggg gtanannnag ttattgttca gcagtggccg ccgccaccaa tcccctctcg ggccttgtta gaagcnggaa ntcnancaac ttggctgnnn aantgctnng ctgtttattn nnt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SKSDLERQES REKTGVFLGS YAINPANGEP VPIFIADYVL AGYGTGAIMA VPGHDQRDWD FARAFGLPIV EVIAGGNISE SAYTGDGILV NSDYLNGMSV PAAKRAIVDR LESAGRGRAR IEFKLRDWLF ARQRYWGE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgaa atcagacctc gagcgccagg agagcaggga aaagaccggc gtcttcttgg gcagctacgc catcaacccg gccaacggtg agccggtgcc gatcttcatc gccgactacg tgctggccgg gtacggtacc ggggcaatca tggcggtgcc gggtcatgac cagcgggact gggacttcgc tcgggcattt ggtctaccga tcgtggaagt aattgccggc ggcaatattt cggaatccgc gtatacaggc gatggcatcc tggtcaactc ggattacctc aatggaatga gcgtgccagc agcaaagcgg gccatcgtcg accggttgga gtccgcgggc cgcggccggg ctcgaatcga attcaaattg cgcgactggc tttttgcgcg gcagcggtat tggggtgaat aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for MytuD.17943.a.A5.GE38386
HARVESTED ON: 8/8/2014
SEQUENCED ON: 8/13/2014
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMQWMMRITA YADRLLDDLD VLDWPEQVKT MQRNWIGRST GAVALFSARA ASDDGFEVDI EVFTTRPDTL FGATYLVLAP EHDLVDELVA ASWPAGVNPL WTYGGGTPGE AIAAYRRAIA AKSDLERQES REKTGVFLGS YAINPANGEP VPIFIADYVL AGYGTGAIMA VPGHDQRDWD FARAFGLPIV EVIAGGNISE SAYTGDGILV NSDYLNGMSV PAAKRAIVDR LESAGRGRAR IEFKLRDWLF ARQRYWGE
Validated NT Sequence
tngatatttt gtttaacttt aagaaggaga tataccatgg ctcatcacca tcaccatcat atgggtaccc tggaagctca gacccagggt cctggttcga tgcaatggat gatgcggatc accgcctatg ccgaccggct gctcgacgac ctggatgtgc tggattggcc tgagcaggtc aagaccatgc agcgcaactg gatcgggcgt tcgacgggtg cggtggcgct gttctcggcg agagcggcca gcgatgacgg gttcgaagtc gacatcgagg tgttcaccac gcggcccgac accttgttcg gcgccacgta tctggtgctg gctcccgagc acgacttggt cgacgagttg gtcgccgcgt cctggccggc tggggtcaac cccttgtgga catacggcgg cggcacacct ggtgaggcca tcgccgccta ccggcgtgcg atcgccgcca aatcagacct cgagcgccag gagagcaggg aaaagaccgg cgtcttcttg ggcagctacg ccatcaaccc ggccaacggt gagccggtgc cgatcttcat cgccgactac gtgctggccg ggtacggtac cggggcaatc atggcggtgc cgggtcatga ccagcgggac tgggacttcg ctcgggcatt tggtctaccg atcgtggaag taattgccgg cggcaatatt tcggaatccg cgtatacagg cgatggcatc ctggtcaact cggattacct caatggaatg agcgtgccag cagcaaagcg ggccatcgtc gaccggttgg agtccgcggg ccgcggccgg gctcgaatcg aattcaaatt gcgcgactgg ctttttgcgc ggcagcggta ttggggtgaa taataaacag cacgaacaag ttctgcagcc aagcttctcg aggatcccnc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc atactagcat acccctgggc nntannnnnt tttnnnntnn nnnaaaaaaa aannnagaca nntntntnnn nnnctcccac acnngtgang nannanannn ntnnncctct gngggcntgt aancggnngg nnnnncannn aaancnnggn ngacnnnnnn nnnngtgcng ttnannnnnn ccnnnnnnct ccnnnnnann nnnnngcann nnnanaantn nnnncngnnn nnnncngg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SQWMMRITAY ADRLLDDLDV LDWPEQVKTM QRNWIGRSTG AVALFSARAA SDDGFEVDIE VFTTRPDTLF GATYLVLAPE HDLVDELVAA SWPAGVNPLW TYGGGTPGEA IAAYRRAIAA KSDLERQESR EKTGVFLGSY AINPANGEPV PIFIADYVLA GYGTGAIMAV PGHDQRDWDF ARAFGLPIVE VIAGGNISES AYTGDGILVN SDYLNGMSVP AAKRAIVDRL ESAGRGRARI EFKLRDWLFA RQRYWGE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgca atggatgatg cggatcaccg cctatgccga ccggctgctc gacgacctgg atgtgctgga ttggcctgag caggtcaaga ccatgcagcg caactggatc gggcgttcga cgggtgcggt ggcgctgttc tcggcgagag cggccagcga tgacgggttc gaagtcgaca tcgaggtgtt caccacgcgg cccgacacct tgttcggcgc cacgtatctg gtgctggctc ccgagcacga cttggtcgac gagttggtcg ccgcgtcctg gccggctggg gtcaacccct tgtggacata cggcggcggc acacctggtg aggccatcgc cgcctaccgg cgtgcgatcg ccgccaaatc agacctcgag cgccaggaga gcagggaaaa gaccggcgtc ttcttgggca gctacgccat caacccggcc aacggtgagc cggtgccgat cttcatcgcc gactacgtgc tggccgggta cggtaccggg gcaatcatgg cggtgccggg tcatgaccag cgggactggg acttcgctcg ggcatttggt ctaccgatcg tggaagtaat tgccggcggc aatatttcgg aatccgcgta tacaggcgat ggcatcctgg tcaactcgga ttacctcaat ggaatgagcg tgccagcagc aaagcgggcc atcgtcgacc ggttggagtc cgcgggccgc ggccgggctc gaatcgaatt caaattgcgc gactggcttt ttgcgcggca gcggtattgg ggtgaataaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for MytuD.17943.a.A4.GE38385
HARVESTED ON: 8/8/2014
SEQUENCED ON: 8/13/2014
EXPECTED MW: 104kDa
OBSERVED MW: 105kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 33
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRLERTWQE NWARLGTFNV PNPVGSLAPP DGAAVPDDKL FVQDMFPYPS GEGLHVGHPL GYIATDVYAR YFRMVGRNVL HALGFDAFGL PAEQYAVQTG THPRTRTEAN VVNFRRQLGR LGFGHDSRRS FSTTDVDFYR WTQWIFLQIY NAWFDTTANK ARPISELVAE FESGARCLDG GRDWAKLTAG ERADVIDEYR LVYRADSLVN WCPGLGTVLA NEEVTADGRS DRGNFPVFRK RLRKWMMRIT AYADRLLDDL DVLDWPEQVK TMQRNWIGRS TGAVALSRRE RQR
Validated NT Sequence
tnnctagata ttttgtttac tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgaggct ggaacggacc tggcaggaaa actgggcccg gctagggacg ttcaacgtgc ccaacccggt cggctcgctg gccccaccgg atggtgccgc ggtgcctgac gacaagctct tcgtgcagga catgttcccc tacccctcgg gtgagggact ccacgttggt catcccctcg gctacatcgc gaccgacgtc tatgcccgct atttccggat ggtgggccgt aatgtgctgc atgcgctagg gttcgacgcg ttcgggctgc ccgccgagca atacgcggta caaaccggca cccatccgcg tacccggacc gaagccaacg tcgtcaactt tcgccgccag ttgggccggc tgggcttcgg ccacgacagc cgacgaagct tctcgaccac cgatgtcgac ttctacaggt ggactcagtg gatcttccta cagatataca acgcgtggtt cgacaccaca gccaacaagg cgcgcccgat atcagagctg gtcgccgaat tcgagtccgg tgcaaggtgt ctcgatggcg gccgggattg ggccaagttg accgcggggg agcgagccga tgtgatcgac gagtaccggc tggtctatcg ggcggattcg ctggtgaact ggtgcccggg gctaggtacg gtgcttgcca acgaagaggt gaccgccgac ggccgcagcg accggggcaa ttttccggtg ttccggaagc ggttgcggaa atggatgatg cggatcaccg cctatgccga ccggctgctc gacgacctgg atgtgctgga ttggcctgag caggtcaaga ccatgcagcg caactggatc gggcgttcga cgggtgcggt ggcgctgtct cggcgagagc ggcagcgatg acgggncgan tcgacatcga gtgttcacca cgcggcccga cnnnntcgcg cacgtatctg gtgctggctn ccgannanna ctngncgacn annngtcgcn cgtctnnnnn nnggncaccc nnnnngnnnn ncgnnnnnnn ctggtgagnn nncnnnanng gcnnnnntng ccgccannnn nnnnnnnnnn nannnnnnna cgnnnntnnc cat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SRLERTWQEN WARLGTFNVP NPVGSLAPPD GAAVPDDKLF VQDMFPYPSG EGLHVGHPLG YIATDVYARY FRMVGRNVLH ALGFDAFGLP AEQYAVQTGT HPRTRTEANV VNFRRQLGRL GFGHDSRRSF STTDVDFYRW TQWIFLQIYN AWFDTTANKA RPISELVAEF ESGARCLDGG RDWAKLTAGE RADVIDEYRL VYRADSLVNW CPGLGTVLAN EEVTADGRSD RGNFPVFRKR LRQWMMRITA YADRLLDDLD VLDWPEQVKT MQRNWIGRST GAVALFSARA ASDDGFEVDI EVFTTRPDTL FGATYLVLAP EHDLVDELVA ASWPAGVNPL WTYGGGTPGE AIAAYRRAIA AKSDLERQES REKTGVFLGS YAINPANGEP VPIFIADYVL AGYGTGAIMA VPGHDQRDWD FARAFGLPIV EVIAGGNISE SAYTGDGILV NSDYLNGMSV PAAKRAIVDR LESAGRGRAR IEFKLRDWLF ARQRYWGEPF PIVYDSDGRP HALDEAALPV ELPDVPDYSP VLFDPDDADS EPSPPLAKAT EWVHVDLDLG DGLKPYSRDT NVMPQWAGSS WYELRYTDPH NSERFCAKEN EAYWMGPRPA EHGPDDPGGV DLYVGGAEHA VLHLLYSRFW HKVLYDLGHV SSREPYRRLV NQGYIQAYAY TDARGSYVPA EQVIERGDRF VYPGPDGEVE VFQEFGKIGK SLKNSVSPDE ICDAYGADTL RVYEMSMGPL EASRPWATKD VVGAYRFLQR VWRLVVDEHT GETRVADGVE LDIDTLRALH RTIVGVSEDF AALRNNTATA KLIEYTNHLT KKHRDAVPRA AVEPLVQMLA PLAPHIAEEL WLRLGNTTSL AHGPFPKADA AYLVDETVEY PVQVNGKVRG RVVVAADTDE ETLKAAVLTD EK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgag gctggaacgg acctggcagg aaaactgggc ccggctaggg acgttcaacg tgcccaaccc ggtcggctcg ctggccccac cggatggtgc cgcggtgcct gacgacaagc tcttcgtgca ggacatgttc ccctacccct cgggtgaggg actccacgtt ggtcatcccc tcggctacat cgcgaccgac gtctatgccc gctatttccg gatggtgggc cgtaatgtgc tgcatgcgct agggttcgac gcgttcgggc tgcccgccga gcaatacgcg gtacaaaccg gcacccatcc gcgtacccgg accgaagcca acgtcgtcaa ctttcgccgc cagttgggcc ggctgggctt cggccacgac agccgacgaa gcttctcgac caccgatgtc gacttctaca ggtggactca gtggatcttc ctacagatat acaacgcgtg gttcgacacc acagccaaca aggcgcgccc gatatcagag ctggtcgccg aattcgagtc cggtgcaagg tgtctcgatg gcggccggga ttgggccaag ttgaccgcgg gggagcgagc cgatgtgatc gacgagtacc ggctggtcta tcgggcggat tcgctggtga actggtgccc ggggctaggt acggtgcttg ccaacgaaga ggtgaccgcc gacggccgca gcgaccgggg caattttccg gtgttccgga agcggttgcg gcaatggatg atgcggatca ccgcctatgc cgaccggctg ctcgacgacc tggatgtgct ggattggcct gagcaggtca agaccatgca gcgcaactgg atcgggcgtt cgacgggtgc ggtggcgctg ttctcggcga gagcggccag cgatgacggg ttcgaagtcg acatcgaggt gttcaccacg cggcccgaca ccttgttcgg cgccacgtat ctggtgctgg ctcccgagca cgacttggtc gacgagttgg tcgccgcgtc ctggccggct ggggtcaacc ccttgtggac atacggcggc ggcacacctg gtgaggccat cgccgcctac cggcgtgcga tcgccgccaa atcagacctc gagcgccagg agagcaggga aaagaccggc gtcttcttgg gcagctacgc catcaacccg gccaacggtg agccggtgcc gatcttcatc gccgactacg tgctggccgg gtacggtacc ggggcaatca tggcggtgcc gggtcatgac cagcgggact gggacttcgc tcgggcattt ggtctaccga tcgtggaagt aattgccggc ggcaatattt cggaatccgc gtatacaggc gatggcatcc tggtcaactc ggattacctc aatggaatga gcgtgccagc agcaaagcgg gccatcgtcg accggttgga gtccgcgggc cgcggccggg ctcgaatcga attcaaattg cgcgactggc tttttgcgcg gcagcggtat tggggtgaac cattcccgat cgtctatgac agcgacgggc gtccgcatgc gctcgacgaa gctgcactgc ccgtcgagct gcctgatgtc ccggactact cgccggtttt gttcgacccc gacgatgcgg acagcgagcc ttcgccccca ctggccaagg cgactgagtg ggtacacgtc gacctggacc tcggtgatgg cctgaagccc tacagccgcg acaccaacgt gatgccgcag tgggcgggca gctcctggta tgaactgcgc tacaccgatc cgcacaactc agaacggttc tgcgccaagg aaaacgaggc ctattggatg ggaccgcggc cggctgagca cggcccggac gaccccggtg gcgtcgactt gtacgtcggc ggtgctgaac acgcggtttt gcacctgctg tattccaggt tctggcacaa ggtcttgtac gacctgggtc acgtcagctc tcgcgagcct taccgcaggc tggtcaatca gggctatatt caagcttacg cttacaccga tgcgcgcgga tcctatgtcc ctgccgagca ggtgatcgaa cgcggtgaca gatttgtcta tcctggacct gacggtgagg tcgaagtttt ccaggaattc ggcaaaatcg gtaagagcct gaagaattcg gtatcgccgg acgaaatctg cgacgcatac ggggcagata cgcttcgggt ttacgagatg tcgatggggc cgctggaggc ttcacgtcca tgggccacaa aggatgttgt cggcgcgtac cgttttctgc agcgggtgtg gcgcttggtc gtcgacgagc acaccggcga aactcgggtg gctgacggcg tggaactcga catcgatacg ctacgggcgt tgcaccgcac catcgtcggc gtgtcagaag actttgcggc acttcgcaat aacaccgcaa cggctaagtt gatcgaatac acgaaccacc tcaccaagaa gcatcgtgat gcggtgcctc gggccgccgt ggagccgctt gtacaaatgc tggctccgct ggccccacat attgccgagg agctgtggct gcgactgggc aacaccacct cgttggcaca cggcccgttc ccgaaggccg atgccgccta cctcgtcgac gagacggtcg agtatccggt gcaggtgaac ggcaaggtac gtggccgggt ggtggtggcc gccgacaccg acgaggaaac gctgaaagcc gccgttctga ccgacgaaaa gtaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for MytuD.17943.a.A3.GE38384
HARVESTED ON: 8/8/2014
SEQUENCED ON: 8/13/2014
EXPECTED MW: 107kDa
OBSERVED MW: 110kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 33
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTESPTAG PGGVPRADDA DSDVPRYRYT AELAARLERT WQENWARLGT FNVPNPVGSL APPDGAAVPD DKLFVQDMFP YPSGEGLHVG HPLGYIATDV YARYFRMVGR NVLHALGFDA FGLPAEQYAV QTGTHPRTRT EANVVNFRRQ LGRLGFGHDS RRSFSTTDVD FYRWTQWIFL QIYNAWFDTT ANKARPISEL VAEFESGARC LDGGRDWAKL TAGERADVID EYRLVYRADS LVNWCPGLGT VLANEEVTAD GRSDRGNFPV FRKRLRQWMM RITAYADRAA RRPGCAGLA
Validated NT Sequence
tnnnntanna taattttgtt taactttaag aaggagatat accatggctc atcaccatca ccatcatatg ggtaccctgg aagctcagac ccagggtcct ggttcgatgg tgaccgaatc gccaaccgct gggcctggcg gcgtgccccg tgccgacgac gcggactccg acgtgccacg gtaccgctat accgccgagc tcgcggctag gctggaacgg acctggcagg aaaactgggc ccggctaggg acgttcaacg tgcccaaccc ggtcggctcg ctggccccac cggatggtgc cgcggtgcct gacgacaagc tcttcgtgca ggacatgttc ccctacccct cgggtgaggg actccacgtt ggtcatcccc tcggctacat cgcgaccgac gtctatgccc gctatttccg gatggtgggc cgtaatgtgc tgcatgcgct agggttcgac gcgttcgggc tgcccgccga gcaatacgcg gtacaaaccg gcacccatcc gcgtacccgg accgaagcca acgtcgtcaa ctttcgccgc cagttgggcc ggctgggctt cggccacgac agccgacgaa gcttctcgac caccgatgtc gacttctaca ggtggactca gtggatcttc ctacagatat acaacgcgtg gttcgacacc acagccaaca aggcgcgccc gatatcagag ctggtcgccg aattcgagtc cggtgcaagg tgtctcgatg gcggccggga ttgggccaag ttgaccgcgg gggagcgagc cgatgtgatc gacgagtacc ggctggtcta tcgggcggat tcgctggtga actggtgccc ggggctaggt acggtgcttg ccaacgaaga ggtgaccgcc gacggccgca gcgaccgggg caattttccg gtgttccgga agcggttgcg gcaatggatg atgcggatca ccgcctatgc cgaccgggct gctcgacgac ctggntgtgc tggattggcc tgagcaggtc aagaccatgc agcgcaactg gatcgggcgt tcgacgggng ccggtggnnc tnntctcgnn agagcggcca gcgatgacgg gnncnagtcg acatcgaggn gttcaccnnn nnncngacnn nnnnnnnccc ncnnnatctg gngctngntc cnannangac ttnnnnnann nnnnnnncgn cgnnnncnnn nnnnnggggg tnnaccccnn nnngnnggnn aca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTESPTAGP GGVPRADDAD SDVPRYRYTA ELAARLERTW QENWARLGTF NVPNPVGSLA PPDGAAVPDD KLFVQDMFPY PSGEGLHVGH PLGYIATDVY ARYFRMVGRN VLHALGFDAF GLPAEQYAVQ TGTHPRTRTE ANVVNFRRQL GRLGFGHDSR RSFSTTDVDF YRWTQWIFLQ IYNAWFDTTA NKARPISELV AEFESGARCL DGGRDWAKLT AGERADVIDE YRLVYRADSL VNWCPGLGTV LANEEVTADG RSDRGNFPVF RKRLRQWMMR ITAYADRLLD DLDVLDWPEQ VKTMQRNWIG RSTGAVALFS ARAASDDGFE VDIEVFTTRP DTLFGATYLV LAPEHDLVDE LVAASWPAGV NPLWTYGGGT PGEAIAAYRR AIAAKSDLER QESREKTGVF LGSYAINPAN GEPVPIFIAD YVLAGYGTGA IMAVPGHDQR DWDFARAFGL PIVEVIAGGN ISESAYTGDG ILVNSDYLNG MSVPAAKRAI VDRLESAGRG RARIEFKLRD WLFARQRYWG EPFPIVYDSD GRPHALDEAA LPVELPDVPD YSPVLFDPDD ADSEPSPPLA KATEWVHVDL DLGDGLKPYS RDTNVMPQWA GSSWYELRYT DPHNSERFCA KENEAYWMGP RPAEHGPDDP GGVDLYVGGA EHAVLHLLYS RFWHKVLYDL GHVSSREPYR RLVNQGYIQA YAYTDARGSY VPAEQVIERG DRFVYPGPDG EVEVFQEFGK IGKSLKNSVS PDEICDAYGA DTLRVYEMSM GPLEASRPWA TKDVVGAYRF LQRVWRLVVD EHTGETRVAD GVELDIDTLR ALHRTIVGVS EDFAALRNNT ATAKLIEYTN HLTKKHRDAV PRAAVEPLVQ MLAPLAPHIA EELWLRLGNT TSLAHGPFPK ADAAYLVDET VEYPVQVNGK VRGRVVVAAD TDEETLKAAV LTDEK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcggt gaccgaatcg ccaaccgctg ggcctggcgg cgtgccccgt gccgacgacg cggactccga cgtgccacgg taccgctata ccgccgagct cgcggctagg ctggaacgga cctggcagga aaactgggcc cggctaggga cgttcaacgt gcccaacccg gtcggctcgc tggccccacc ggatggtgcc gcggtgcctg acgacaagct cttcgtgcag gacatgttcc cctacccctc gggtgaggga ctccacgttg gtcatcccct cggctacatc gcgaccgacg tctatgcccg ctatttccgg atggtgggcc gtaatgtgct gcatgcgcta gggttcgacg cgttcgggct gcccgccgag caatacgcgg tacaaaccgg cacccatccg cgtacccgga ccgaagccaa cgtcgtcaac tttcgccgcc agttgggccg gctgggcttc ggccacgaca gccgacgaag cttctcgacc accgatgtcg acttctacag gtggactcag tggatcttcc tacagatata caacgcgtgg ttcgacacca cagccaacaa ggcgcgcccg atatcagagc tggtcgccga attcgagtcc ggtgcaaggt gtctcgatgg cggccgggat tgggccaagt tgaccgcggg ggagcgagcc gatgtgatcg acgagtaccg gctggtctat cgggcggatt cgctggtgaa ctggtgcccg gggctaggta cggtgcttgc caacgaagag gtgaccgccg acggccgcag cgaccggggc aattttccgg tgttccggaa gcggttgcgg caatggatga tgcggatcac cgcctatgcc gaccggctgc tcgacgacct ggatgtgctg gattggcctg agcaggtcaa gaccatgcag cgcaactgga tcgggcgttc gacgggtgcg gtggcgctgt tctcggcgag agcggccagc gatgacgggt tcgaagtcga catcgaggtg ttcaccacgc ggcccgacac cttgttcggc gccacgtatc tggtgctggc tcccgagcac gacttggtcg acgagttggt cgccgcgtcc tggccggctg gggtcaaccc cttgtggaca tacggcggcg gcacacctgg tgaggccatc gccgcctacc ggcgtgcgat cgccgccaaa tcagacctcg agcgccagga gagcagggaa aagaccggcg tcttcttggg cagctacgcc atcaacccgg ccaacggtga gccggtgccg atcttcatcg ccgactacgt gctggccggg tacggtaccg gggcaatcat ggcggtgccg ggtcatgacc agcgggactg ggacttcgct cgggcatttg gtctaccgat cgtggaagta attgccggcg gcaatatttc ggaatccgcg tatacaggcg atggcatcct ggtcaactcg gattacctca atggaatgag cgtgccagca gcaaagcggg ccatcgtcga ccggttggag tccgcgggcc gcggccgggc tcgaatcgaa ttcaaattgc gcgactggct ttttgcgcgg cagcggtatt ggggtgaacc attcccgatc gtctatgaca gcgacgggcg tccgcatgcg ctcgacgaag ctgcactgcc cgtcgagctg cctgatgtcc cggactactc gccggttttg ttcgaccccg acgatgcgga cagcgagcct tcgcccccac tggccaaggc gactgagtgg gtacacgtcg acctggacct cggtgatggc ctgaagccct acagccgcga caccaacgtg atgccgcagt gggcgggcag ctcctggtat gaactgcgct acaccgatcc gcacaactca gaacggttct gcgccaagga aaacgaggcc tattggatgg gaccgcggcc ggctgagcac ggcccggacg accccggtgg cgtcgacttg tacgtcggcg gtgctgaaca cgcggttttg cacctgctgt attccaggtt ctggcacaag gtcttgtacg acctgggtca cgtcagctct cgcgagcctt accgcaggct ggtcaatcag ggctatattc aagcttacgc ttacaccgat gcgcgcggat cctatgtccc tgccgagcag gtgatcgaac gcggtgacag atttgtctat cctggacctg acggtgaggt cgaagttttc caggaattcg gcaaaatcgg taagagcctg aagaattcgg tatcgccgga cgaaatctgc gacgcatacg gggcagatac gcttcgggtt tacgagatgt cgatggggcc gctggaggct tcacgtccat gggccacaaa ggatgttgtc ggcgcgtacc gttttctgca gcgggtgtgg cgcttggtcg tcgacgagca caccggcgaa actcgggtgg ctgacggcgt ggaactcgac atcgatacgc tacgggcgtt gcaccgcacc atcgtcggcg tgtcagaaga ctttgcggca cttcgcaata acaccgcaac ggctaagttg atcgaataca cgaaccacct caccaagaag catcgtgatg cggtgcctcg ggccgccgtg gagccgcttg tacaaatgct ggctccgctg gccccacata ttgccgagga gctgtggctg cgactgggca acaccacctc gttggcacac ggcccgttcc cgaaggccga tgccgcctac ctcgtcgacg agacggtcga gtatccggtg caggtgaacg gcaaggtacg tggccgggtg gtggtggccg ccgacaccga cgaggaaacg ctgaaagccg ccgttctgac cgacgaaaag taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for MytuD.17943.a.A5.PW37567
PURIFICATION DATe: 9/29/2014
CONCENTRATION: 13.4mg/ml
OBSERVED MW: 30kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMQWMMRITA YADRLLDDLD VLDWPEQVKT MQRNWIGRST GAVALFSARA ASDDGFEVDI EVFTTRPDTL FGATYLVLAP EHDLVDELVA ASWPAGVNPL WTYGGGTPGE AIAAYRRAIA AKSDLERQES REKTGVFLGS YAINPANGEP VPIFIADYVL AGYGTGAIMA VPGHDQRDWD FARAFGLPIV EVIAGGNISE SAYTGDGILV NSDYLNGMSV PAAKRAIVDR LESAGRGRAR IEFKLRDWLF ARQRYWGE
Validated NT Sequence
tngatatttt gtttaacttt aagaaggaga tataccatgg ctcatcacca tcaccatcat atgggtaccc tggaagctca gacccagggt cctggttcga tgcaatggat gatgcggatc accgcctatg ccgaccggct gctcgacgac ctggatgtgc tggattggcc tgagcaggtc aagaccatgc agcgcaactg gatcgggcgt tcgacgggtg cggtggcgct gttctcggcg agagcggcca gcgatgacgg gttcgaagtc gacatcgagg tgttcaccac gcggcccgac accttgttcg gcgccacgta tctggtgctg gctcccgagc acgacttggt cgacgagttg gtcgccgcgt cctggccggc tggggtcaac cccttgtgga catacggcgg cggcacacct ggtgaggcca tcgccgccta ccggcgtgcg atcgccgcca aatcagacct cgagcgccag gagagcaggg aaaagaccgg cgtcttcttg ggcagctacg ccatcaaccc ggccaacggt gagccggtgc cgatcttcat cgccgactac gtgctggccg ggtacggtac cggggcaatc atggcggtgc cgggtcatga ccagcgggac tgggacttcg ctcgggcatt tggtctaccg atcgtggaag taattgccgg cggcaatatt tcggaatccg cgtatacagg cgatggcatc ctggtcaact cggattacct caatggaatg agcgtgccag cagcaaagcg ggccatcgtc gaccggttgg agtccgcggg ccgcggccgg gctcgaatcg aattcaaatt gcgcgactgg ctttttgcgc ggcagcggta ttggggtgaa taataaacag cacgaacaag ttctgcagcc aagcttctcg aggatcccnc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc atactagcat acccctgggc nntannnnnt tttnnnntnn nnnaaaaaaa aannnagaca nntntntnnn nnnctcccac acnngtgang nannanannn ntnnncctct gngggcntgt aancggnngg nnnnncannn aaancnnggn ngacnnnnnn nnnngtgcng ttnannnnnn ccnnnnnnct ccnnnnnann nnnnngcann nnnanaantn nnnncngnnn nnnncngg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SQWMMRITAY ADRLLDDLDV LDWPEQVKTM QRNWIGRSTG AVALFSARAA SDDGFEVDIE VFTTRPDTLF GATYLVLAPE HDLVDELVAA SWPAGVNPLW TYGGGTPGEA IAAYRRAIAA KSDLERQESR EKTGVFLGSY AINPANGEPV PIFIADYVLA GYGTGAIMAV PGHDQRDWDF ARAFGLPIVE VIAGGNISES AYTGDGILVN SDYLNGMSVP AAKRAIVDRL ESAGRGRARI EFKLRDWLFA RQRYWGE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGCA ATGGATGATG CGGATCACCG CCTATGCCGA CCGGCTGCTC GACGACCTGG ATGTGCTGGA TTGGCCTGAG CAGGTCAAGA CCATGCAGCG CAACTGGATC GGGCGTTCGA CGGGTGCGGT GGCGCTGTTC TCGGCGAGAG CGGCCAGCGA TGACGGGTTC GAAGTCGACA TCGAGGTGTT CACCACGCGG CCCGACACCT TGTTCGGCGC CACGTATCTG GTGCTGGCTC CCGAGCACGA CTTGGTCGAC GAGTTGGTCG CCGCGTCCTG GCCGGCTGGG GTCAACCCCT TGTGGACATA CGGCGGCGGC ACACCTGGTG AGGCCATCGC CGCCTACCGG CGTGCGATCG CCGCCAAATC AGACCTCGAG CGCCAGGAGA GCAGGGAAAA GACCGGCGTC TTCTTGGGCA GCTACGCCAT CAACCCGGCC AACGGTGAGC CGGTGCCGAT CTTCATCGCC GACTACGTGC TGGCCGGGTA CGGTACCGGG GCAATCATGG CGGTGCCGGG TCATGACCAG CGGGACTGGG ACTTCGCTCG GGCATTTGGT CTACCGATCG TGGAAGTAAT TGCCGGCGGC AATATTTCGG AATCCGCGTA TACAGGCGAT GGCATCCTGG TCAACTCGGA TTACCTCAAT GGAATGAGCG TGCCAGCAGC AAAGCGGGCC ATCGTCGACC GGTTGGAGTC CGCGGGCCGC GGCCGGGCTC GAATCGAATT CAAATTGCGC GACTGGCTTT TTGCGCGGCA GCGGTATTGG GGTGAATAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA
Details for MytuD.17943.a.A8.PW37568
PURIFICATION DATe: 9/29/2014
CONCENTRATION: 20mg/ml
OBSERVED MW: 33kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMQWMMRITA YADRLLDDLD VLDWPEQVKT MQRNWIGRST GAVALFSARA ASDDGFEVDI EVFTTRPDTL FGATYLVLAP EHDLVDELVA ASWPAGVNPL WTYGGGTPGE AIAAYRRAIA AKSDLERQES REKTGVFLGS YAINPANGEP VPIFIADYVL AGYGTGAIMA VPGHDQRDWD FARAFGLPIV EVIAGGNISE SAYTGDGILV NSDYLNGMSV PAAKRAIVDR LESAGRGRAR IEFKLRDWLF ARQRYWGEPF PIVYDSDGRP HALDEAALPV ELPDL
Validated NT Sequence
cnnnannntn attttgttta actttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgcaa tggatgatgc ggatcaccgc ctatgccgac cggctgctcg acgacctgga tgtgctggat tggcctgagc aggtcaagac catgcagcgc aactggatcg ggcgttcgac gggtgcggtg gcgctgttct cggcgagagc ggccagcgat gacgggttcg aagtcgacat cgaggtgttc accacgcggc ccgacacctt gttcggcgcc acgtatctgg tgctggctcc cgagcacgac ttggtcgacg agttggtcgc cgcgtcctgg ccggctgggg tcaacccctt gtggacatac ggcggcggca cacctggtga ggccatcgcc gcctaccggc gtgcgatcgc cgccaaatca gacctcgagc gccaggagag cagggaaaag accggcgtct tcttgggcag ctacgccatc aacccggcca acggtgagcc ggtgccgatc ttcatcgccg actacgtgct ggccgggtac ggtaccgggg caatcatggc ggtgccgggt catgaccagc gggactggga cttcgctcgg gcatttggtc taccgatcgt ggaagtaatt gccggcggca atatttcgga atccgcgtat acaggcgatg gcatcctggt caactcggat tacctcaatg gaatgagcgt gccagcagca aagcgggcca tcgtcgaccg gttggagtcc gcgggccgcg gccgggctcg aatcgaattc aaattgcgcg actggctttt tgcgcggcag cggtattggg gtgaaccatt cccgatcgtc tatgacagcg acgggcgtcc gcatgcgctc gacgaagctg cactgcccgt cgagctgcct gatttataaa cagcacgaac aagttctgca gccnagcttc tcgagntccn ctgctaacaa agcccgaaan gnnctgagtt gctgctgcac gctgagcata ctagcatanc cctnnnnncn acnnnntgnn ngnggaaann nnagaagnnn nannnantnn nnnntngnnc nncnggngnn nnngantnnn ttnnnncncn nnng
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SQWMMRITAY ADRLLDDLDV LDWPEQVKTM QRNWIGRSTG AVALFSARAA SDDGFEVDIE VFTTRPDTLF GATYLVLAPE HDLVDELVAA SWPAGVNPLW TYGGGTPGEA IAAYRRAIAA KSDLERQESR EKTGVFLGSY AINPANGEPV PIFIADYVLA GYGTGAIMAV PGHDQRDWDF ARAFGLPIVE VIAGGNISES AYTGDGILVN SDYLNGMSVP AAKRAIVDRL ESAGRGRARI EFKLRDWLFA RQRYWGEPFP IVYDSDGRPH ALDEAALPVE LPDVPDY
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGCA ATGGATGATG CGGATCACCG CCTATGCCGA CCGGCTGCTC GACGACCTGG ATGTGCTGGA TTGGCCTGAG CAGGTCAAGA CCATGCAGCG CAACTGGATC GGGCGTTCGA CGGGTGCGGT GGCGCTGTTC TCGGCGAGAG CGGCCAGCGA TGACGGGTTC GAAGTCGACA TCGAGGTGTT CACCACGCGG CCCGACACCT TGTTCGGCGC CACGTATCTG GTGCTGGCTC CCGAGCACGA CTTGGTCGAC GAGTTGGTCG CCGCGTCCTG GCCGGCTGGG GTCAACCCCT TGTGGACATA CGGCGGCGGC ACACCTGGTG AGGCCATCGC CGCCTACCGG CGTGCGATCG CCGCCAAATC AGACCTCGAG CGCCAGGAGA GCAGGGAAAA GACCGGCGTC TTCTTGGGCA GCTACGCCAT CAACCCGGCC AACGGTGAGC CGGTGCCGAT CTTCATCGCC GACTACGTGC TGGCCGGGTA CGGTACCGGG GCAATCATGG CGGTGCCGGG TCATGACCAG CGGGACTGGG ACTTCGCTCG GGCATTTGGT CTACCGATCG TGGAAGTAAT TGCCGGCGGC AATATTTCGG AATCCGCGTA TACAGGCGAT GGCATCCTGG TCAACTCGGA TTACCTCAAT GGAATGAGCG TGCCAGCAGC AAAGCGGGCC ATCGTCGACC GGTTGGAGTC CGCGGGCCGC GGCCGGGCTC GAATCGAATT CAAATTGCGC GACTGGCTTT TTGCGCGGCA GCGGTATTGG GGTGAACCAT TCCCGATCGT CTATGACAGC GACGGGCGTC CGCATGCGCT CGACGAAGCT GCACTGCCCG TCGAGCTGCC TGATGTCCCG GACTACTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA