MytuD.17792.a

Nonspecific lipid-transfer protein (ltp2) (Rv3540c)

CENTER ID: MytuD.17792.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.17792.a.A1.GE30700 full length 1 386
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.3954
RefSeq: NP_218057.1
TubercuList: Rv3540c
MTB Network Portal: Rv3540c
UniProt: I6Y3T7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLSGQAAIVG IGATDFSKNS GRSELRLAAE AVLDALADAG LSPTDVDGLT TFTMDTNTEI AVARAAGIGE LTFFSKIHYG GGAACATVQH AAMAVATGVA DVVVAYRAFN ERSGMRFGQV QTRLTENADS TGVDNSFSYP HGLSTPAAQV AMIARRYMHL SGATSRDFGA VSVADRKHAA NNPKAYFYGK PITIEDHQNS RWIAEPLRLL DCCQETDGAV AIVVTSAARA RDLKQRPVVI EAAAQGCSPD QYTMVSYYRP ELDGLPEMGL VGRQLWAQSG LTPADVQTAV LYDHFTPFTL IQLEELGFCG KGEAKDFIAD GAIEVGGRLP INTHGGQLGE AYIHGMNGIA EGVRQLRGTS VNPVAGVEHV LVTAGTGVPT SGLILG
NT Sequence
gtgttatcgg gtcaggcggc catcgtcggt atcggcgcca ccgacttttc gaagaactcc ggtcgaagtg agctgcggct ggcggccgag gcggtgttgg atgcgttggc cgatgcgggc ctgagcccga ccgatgtcga cgggctgacc acgttcacga tggacaccaa caccgaaatc gccgtggcgc gtgcggccgg catcggcgag ctgacgttct tctccaagat ccactacggc ggtggcgccg catgtgcgac cgtgcagcac gccgctatgg cagtggccac cggggtggct gacgtcgtgg tggcgtatcg ggcattcaac gaacgatccg gcatgcggtt cggtcaggtg caaactcgtt tgaccgagaa tgccgactcc accggcgtgg acaattcgtt ttcgtatccg cacgggctct ccacgcccgc cgcgcaagtg gcgatgatcg ctcgccggta catgcacctg tctggtgcga ccagccggga cttcggtgct gtctcggtgg ccgaccgcaa gcatgccgcc aacaacccca aggcgtactt ctacggcaag ccgataacca ttgaggacca ccagaattcg aggtggatcg ccgagccgct gcggctgctg gactgctgcc aggagaccga cggcgcggtc gcgatcgtgg tgacgtcagc tgcgcgcgca cgggacctca agcagcgccc ggtggtcatt gaggcggctg cgcagggctg cagtccagac cagtacacga tggtcagcta ctaccggccg gaactcgacg gcctgcccga gatgggcctg gtgggccggc agctatgggc gcagtcgggg ctgacgccgg ccgatgtcca gaccgcagtc ctctacgacc acttcacgcc gtttaccctg attcagttgg aggagttggg attctgcggc aagggcgaag cgaaagactt catcgccgac ggcgcgatcg aggtgggcgg gcggctgccc atcaacaccc acggcggtca actcggcgaa gcctacatcc atggcatgaa cggcatcgcg gagggggtgc ggcagctgcg cggcacctcg gtgaacccgg tggcgggcgt cgagcatgtg ctcgtcaccg cgggcaccgg ggtgcctacg tccgggctga tcctgggtta a
Details for MytuD.17792.a.A1.GE30700
HARVESTED ON: 11/9/2010
SEQUENCED ON: 11/15/2010
EXPECTED MW: 42kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 83
PERCENT COVERAGE: 93
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVLSGQAAI VGIGATDFSK NSGRSELRLA AEAVLDALAD AGLSPTDVDG LTTFTMDTNT EIAVARAAGI GELTFFSKIH YGGGAACATV QHAAMAVATG VADVVVAYRA FNERSGMRFG QVQTRLTENA DSTGVDNSFS YPHGLSTPAA QVAMIARRYM HLSGATSRDF GAVSVADRKH AANNPKAYFY GKPITIEDHQ NSRWIAEPLR LLDCCQETDG AVAIVVTSAA RARDLKQRPV VIEAAAQGCS PXQYTMVSYY RPELDXLPEX GLVGRQLWAQ SGLXXXDVQT AXXYDHFTXF TLXXXXEXGF CGXXXXKXXI XXXRSXXXXX XXXXXXXXXX XXXXXXXXGX XXXXXXXXXX X
Validated NT Sequence
tttactttaa gaaggagata taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg gtgttatcgg gtcaggcggc catcgtcggt atcggcgcca ccgacttttc gaagaactcc ggtcgaagtg agctgcggct ggcggccgag gcggtgttgg atgcgttggc cgatgcgggc ctgagcccga ccgatgtcga cgggctgacc acgttcacga tggacaccaa caccgaaatc gccgtggcgc gtgcggccgg catcggcgag ctgacgttct tctccaagat ccactacggc ggtggcgccg catgtgcgac cgtgcagcac gccgctatgg cagtggccac cggggtggct gacgtcgtgg tggcgtatcg ggcattcaac gaacgatccg gcatgcggtt cggtcaggtg caaactcgtt tgaccgagaa tgccgactcc accggcgtgg acaattcgtt ttcgtatccg cacgggctct ccacgcccgc cgcgcaagtg gcgatgatcg ctcgccggta catgcacctg tctggtgcga ccagccggga cttcggtgct gtctcggtgg ccgaccgcaa gcatgccgcc aacaacccca aggcgtactt ctacggcaag ccgataacca ttgaggacca ccagaattcg aggtggatcg ccgagccgct gcggctgctg gactgctgcc aggagaccga cggcgcggtc gcgatcgtgg tgacgtcagc tgcgcgcgca cgggacctca agcagcgccc ggtggtcatt gaggcggctg cgcagggctg cagtccagan cagtacacga tggtcagcta ctaccggccg gaactcgacn gcctgcccga gangggcctg gtgggccggc agctatgggc gcagtcnggg ctgangcnnn ccgatgtcca gaccgcannc cnctacgacc acttcacgnc gtttaccctg annnnnnngg nngagtnggg attctgcggn nnggnnnaan cnaaagnnnt natcnnnnan nnncgatcnn nngnngnnnn nncnntnann nnnngnnnna ntngnnaanc nncanncatn nnnnnnngna tnnnnnnngg gnnnnnnnnc nnnnnnnnnc nnnnnnnnnn gnnnnnnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVLSGQAAI VGIGATDFSK NSGRSELRLA AEAVLDALAD AGLSPTDVDG LTTFTMDTNT EIAVARAAGI GELTFFSKIH YGGGAACATV QHAAMAVATG VADVVVAYRA FNERSGMRFG QVQTRLTENA DSTGVDNSFS YPHGLSTPAA QVAMIARRYM HLSGATSRDF GAVSVADRKH AANNPKAYFY GKPITIEDHQ NSRWIAEPLR LLDCCQETDG AVAIVVTSAA RARDLKQRPV VIEAAAQGCS PDQYTMVSYY RPELDGLPEM GLVGRQLWAQ SGLTPADVQT AVLYDHFTPF TLIQLEELGF CGKGEAKDFI ADGAIEVGGR LPINTHGGQL GEAYIHGMNG IAEGVRQLRG TSVNPVAGVE HVLVTAGTGV PTSGLILG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgttatcg ggtcaggcgg ccatcgtcgg tatcggcgcc accgactttt cgaagaactc cggtcgaagt gagctgcggc tggcggccga ggcggtgttg gatgcgttgg ccgatgcggg cctgagcccg accgatgtcg acgggctgac cacgttcacg atggacacca acaccgaaat cgccgtggcg cgtgcggccg gcatcggcga gctgacgttc ttctccaaga tccactacgg cggtggcgcc gcatgtgcga ccgtgcagca cgccgctatg gcagtggcca ccggggtggc tgacgtcgtg gtggcgtatc gggcattcaa cgaacgatcc ggcatgcggt tcggtcaggt gcaaactcgt ttgaccgaga atgccgactc caccggcgtg gacaattcgt tttcgtatcc gcacgggctc tccacgcccg ccgcgcaagt ggcgatgatc gctcgccggt acatgcacct gtctggtgcg accagccggg acttcggtgc tgtctcggtg gccgaccgca agcatgccgc caacaacccc aaggcgtact tctacggcaa gccgataacc attgaggacc accagaattc gaggtggatc gccgagccgc tgcggctgct ggactgctgc caggagaccg acggcgcggt cgcgatcgtg gtgacgtcag ctgcgcgcgc acgggacctc aagcagcgcc cggtggtcat tgaggcggct gcgcagggct gcagtccaga ccagtacacg atggtcagct actaccggcc ggaactcgac ggcctgcccg agatgggcct ggtgggccgg cagctatggg cgcagtcggg gctgacgccg gccgatgtcc agaccgcagt cctctacgac cacttcacgc cgtttaccct gattcagttg gaggagttgg gattctgcgg caagggcgaa gcgaaagact tcatcgccga cggcgcgatc gaggtgggcg ggcggctgcc catcaacacc cacggcggtc aactcggcga agcctacatc catggcatga acggcatcgc ggagggggtg cggcagctgc gcggcacctc ggtgaacccg gtggcgggcg tcgagcatgt gctcgtcacc gcgggcaccg gggtgcctac gtccgggctg atcctgggtt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa