MytuD.17784.d

probable membrane-anchored mycosin MYCP4 (serine protease) (Rv3449)

CENTER ID: MytuD.17784.d
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.17784.d.A2.GE30698 soluble mature protein 29 418
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.3854
RefSeq: NP_217966.1
TubercuList: Rv3449
MTB Network Portal: Rv3449
UniProt: I6YC58

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTSRTLRLL VVSALATLSG LGTPVAHAVS PPPIDERWLP ESALPAPPRP TVQREVCTEV TAESGRAFGR AERSAQLADL DQVWRLTRGA GQRVAVIDTG VARHRRLPKV VAGGDYVFTG DGTADCDAHG TLVAGIIAAA PDAQSDNFSG VAPDVTLISI RQSSSKFAPV GDPSSTGVGD VDTMAKAVRT AADLGASVIN ISSIACVPAA AAPDDRALGA ALAYAVDVKN AVIVAAAGNT GGAAQCPPQA PGVTRDSVTV AVSPAWYDDY VLTVGSVNAQ GEPSAFTLAG PWVDVAATGE AVTSLSPFGD GTVNRLGGQH GSIPISGTSY AAPVVSGLAA LIRARFPTLT ARQVMQRIES TAHHPPAGWD PLVGNGTVDA LAAVSSDSIP QAGTATSDPA PVAVPVPRRS TPGPSDRRAL HTAFAGAAIC LLALMATLAT ASRRLRPGRN GIAGD
NT Sequence
atgaccacgt cccgtaccct gcgcctgctg gtggtatcag cgctcgcgac gctgtctggg ttgggaacgc cggttgccca cgcggtttcg ccgccgccga tcgacgaaag atggctaccc gaatctgcgc tgccggcgcc gccgcggccg accgtacaac gtgaggtatg caccgaggtc accgccgaat cgggacgggc tttcggccgg gctgagcggt ccgctcaact cgccgacctc gaccaggtct ggcgactcac ccgcggcgcc ggccaacggg tcgcggtcat cgacaccggc gttgcgcgcc atcgacggtt gcccaaggtg gttgccggcg gtgactatgt cttcaccggg gacggcaccg cggattgcga tgcacacggc acgctggtgg ccggaattat cgcggccgca ccggatgcgc aaagcgacaa tttcagcggg gtggcacccg atgtcacctt gatcagcatt cgccagtcca gcagcaagtt cgcaccggtc ggcgacccgt ccagcacagg tgttggtgac gtcgacacca tggcgaaggc cgtgcggacg gccgccgacc tcggcgcgtc ggtgatcaac atctcgtcga ttgcctgcgt tccggccgcg gctgcgccgg acgaccgcgc gctaggtgcc gctttggcct atgcggtcga tgtcaagaac gccgtcatcg tggccgcggc cggcaatacc ggcggcgccg cgcagtgtcc gccgcaggcc cccggggtaa cccgggacag cgtcacggtt gcggtgagtc cggcctggta cgacgactac gtgctgaccg taggttcggt gaacgcccaa ggcgaaccct cggcattcac tctcgccggc ccctgggtgg atgtcgccgc caccggcgag gcggtgacct cgctcagccc gttcggtgac gggaccgtga acaggcttgg cggacagcat ggttcgattc cgatatccgg aaccagttat gcggcgccgg tcgtcagcgg cctggccgcc ctgatccggg cccgctttcc gacgttgacc gcacggcagg tgatgcagcg catcgaatct accgcgcatc acccacccgc cggatgggat ccgctcgtcg gcaacggcac ggtcgatgcc ctggctgcgg tcagcagcga ctcgattccg caggccggca ccgcaacgag cgaccccgct ccggtggcgg tgccggtccc taggcggtca acgcccggcc catcggatcg ccgcgcccta cacaccgcct ttgctggtgc cgcgatctgc ctgctcgcgc tgatggcaac cctggccacc gccagccgcc ggctacggcc cgggcgcaac ggtatcgcgg gcgactga
Details for MytuD.17784.d.A2.GE30698
HARVESTED ON: 11/9/2010
SEQUENCED ON: 1/13/2011
EXPECTED MW: 41kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 95
PERCENT COVERAGE: 72
Validated AA Sequence
MGTLEAQTQN PGSMVSPPPI DERWLPESAL PAPPRPTVQR EVCTEVTAES GRAFGRAERS AQLADLDQVW RLTRGAGQRV AVIDTGVARH RRLPKVVAGG DYVFTGDGTA DCDAHGTLVA GIIAAAPDAQ SDNFSGVAPD VTLISIRQSS SKFAPVGDPS STGVGDVDTM AKAVRTAADL GASVINISSI ACVPAAAAPD DRALGAALAY AVDVKNAVIV AAXGNTGGAA QCPPQAPGVT RDSVTVAVSP AWYDDYVLTV GSVNAQGEPS AFTLXGPWVD VXATXEXXXS LXXXGXXP
Validated NT Sequence
cnnnnnnnnn ncngnnnnnn nnngcgnnnn nnnnnnnngn nccntgnnnn atcaccntca ccatcatatg ggtaccctgg aagctcagac ccagaatcct ggttcgatgg tttcgccgcc gccgatcgac gaaagatggc tacccgaatc tgcgctgccg gcgccgccgc ggccgaccgt acaacgtgag gtatgcaccg aggtcaccgc cgaatcggga cgggctttcg gccgggctga gcggtccgct caactcgccg acctcgacca ggtctggcga ctcacccgcg gcgccggcca acgggtcgcg gtcatcgaca ccggcgttgc gcgccatcga cggttgccca aggtggttgc cggcggtgac tatgtcttca ccggggacgg caccgcggat tgcgatgcac acggcacgct ggtggccgga attatcgcgg ccgcaccgga tgcgcaaagc gacaatttca gcggggtggc acccgatgtc accttgatca gcattcgcca gtccagcagc aagttcgcac cggtcggcga cccgtccagc acaggtgttg gtgacgtcga caccatggcg aaggccgtgc ggacggccgc cgacctcggc gcgtcggtga tcaacatctc gtcgattgcc tgcgttccgg ccgcggctgc gccggacgac cgcgcgctag gtgccgcttt ggcctatgcg gtcgatgtca agaacgccgt catcgtggcc gcggnnggca ataccggcgg cgccgcgcag tgtccgccgc aggcccccgg ggtaacccgg gacagcgtca cggttgcggt gagtccggcc tggtacgacg actacgtgct gaccgtaggt tcggtgaacg cccaaggcga accctcggca ttcactctcg nnggcccntg ggtggatgtc nncgccaccn gcgaggngnt gncctcgctn ancnngntcg gngannnncc gtgancaggc ttggcnnana gcnnnnnnga tnnnganntn cnnnnntnnt nnngnnnnnt cgncannnnn nnnnnccnga nnngnnnnnt nnnnnntnac nnnngnnnnn nanncannnn atcnnnntan nnnnannanc nnnnnnnnnn nnncnnnnnn nngnnnnnng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSPPPIDE RWLPESALPA PPRPTVQREV CTEVTAESGR AFGRAERSAQ LADLDQVWRL TRGAGQRVAV IDTGVARHRR LPKVVAGGDY VFTGDGTADC DAHGTLVAGI IAAAPDAQSD NFSGVAPDVT LISIRQSSSK FAPVGDPSST GVGDVDTMAK AVRTAADLGA SVINISSIAC VPAAAAPDDR ALGAALAYAV DVKNAVIVAA AGNTGGAAQC PPQAPGVTRD SVTVAVSPAW YDDYVLTVGS VNAQGEPSAF TLAGPWVDVA ATGEAVTSLS PFGDGTVNRL GGQHGSIPIS GTSYAAPVVS GLAALIRARF PTLTARQVMQ RIESTAHHPP AGWDPLVGNG TVDALAAVSS DSIPQAGTAT SDPAPVAVPV PRRSTPGPSD RR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtttcgccg ccgccgatcg acgaaagatg gctacccgaa tctgcgctgc cggcgccgcc gcggccgacc gtacaacgtg aggtatgcac cgaggtcacc gccgaatcgg gacgggcttt cggccgggct gagcggtccg ctcaactcgc cgacctcgac caggtctggc gactcacccg cggcgccggc caacgggtcg cggtcatcga caccggcgtt gcgcgccatc gacggttgcc caaggtggtt gccggcggtg actatgtctt caccggggac ggcaccgcgg attgcgatgc acacggcacg ctggtggccg gaattatcgc ggccgcaccg gatgcgcaaa gcgacaattt cagcggggtg gcacccgatg tcaccttgat cagcattcgc cagtccagca gcaagttcgc accggtcggc gacccgtcca gcacaggtgt tggtgacgtc gacaccatgg cgaaggccgt gcggacggcc gccgacctcg gcgcgtcggt gatcaacatc tcgtcgattg cctgcgttcc ggccgcggct gcgccggacg accgcgcgct aggtgccgct ttggcctatg cggtcgatgt caagaacgcc gtcatcgtgg ccgcggccgg caataccggc ggcgccgcgc agtgtccgcc gcaggccccc ggggtaaccc gggacagcgt cacggttgcg gtgagtccgg cctggtacga cgactacgtg ctgaccgtag gttcggtgaa cgcccaaggc gaaccctcgg cattcactct cgccggcccc tgggtggatg tcgccgccac cggcgaggcg gtgacctcgc tcagcccgtt cggtgacggg accgtgaaca ggcttggcgg acagcatggt tcgattccga tatccggaac cagttatgcg gcgccggtcg tcagcggcct ggccgccctg atccgggccc gctttccgac gttgaccgca cggcaggtga tgcagcgcat cgaatctacc gcgcatcacc cacccgccgg atgggatccg ctcgtcggca acggcacggt cgatgccctg gctgcggtca gcagcgactc gattccgcag gccggcaccg caacgagcga ccccgctccg gtggcggtgc cggtccctag gcggtcaacg cccggcccat cggatcgccg ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa