MytuD.17779.a

Probable RNA polymerase sigma-D factor

CENTER ID: MytuD.17779.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.17779.a.A1.GE31027 Full length( MytuD.17779.a ) 1 212
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.3811
RefSeq: NP_217931.1
TubercuList: Rv3414c
MTB Network Portal: Rv3414c
UniProt: P9WGG9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVDPGVSPGC VRFVTLEISP SMTMQGERLD AVVAEAVAGD RNALREVLET IRPIVVRYCR ARVGTVERSG LSADDVAQEV CLATITALPR YRDRGRPFLA FLYGIAAHKV ADAHRAAGRD RAYPAETLPE RWSADAGPEQ MAIEADSVTR MNELLEILPA KQREILILRV VVGLSAEETA AAVGSTTGAV RVAQHRALQR LKDEIVAAGD YA
NT Sequence
ATGGTCGATC CGGGAGTTAG CCCGGGATGT GTCCGCTTCG TAACGTTGGA GATATCGCCG TCGATGACAA TGCAAGGGGA ACGTCTCGAC GCTGTGGTTG CGGAGGCCGT GGCAGGAGAC CGGAACGCGC TTCGGGAGGT GCTGGAGACC ATCCGCCCGA TCGTCGTGCG ATATTGCCGA GCGCGAGTCG GCACGGTCGA GCGGAGCGGC CTGTCAGCAG ATGACGTGGC ACAGGAGGTG TGCTTGGCCA CCATAACGGC GCTGCCGCGC TATCGGGACC GCGGCCGGCC ATTCCTGGCG TTTCTGTACG GCATCGCGGC GCACAAGGTT GCCGACGCCC ATCGGGCAGC CGGCCGTGAC CGGGCCTATC CCGCCGAAAC GCTTCCTGAG CGCTGGTCAG CCGACGCCGG CCCGGAGCAG ATGGCCATCG AGGCCGATTC GGTCACCCGG ATGAACGAAT TGCTTGAGAT CTTGCCGGCC AAGCAACGCG AGATCCTCAT TCTGCGTGTT GTCGTCGGCC TGTCCGCGGA AGAGACCGCC GCCGCCGTCG GCAGCACCAC GGGGGCGGTC CGGGTGGCCC AACACCGTGC ACTTCAGCGG CTGAAGGACG AGATTGTTGC GGCAGGTGAC TATGCG
Details for MytuD.17779.a.A1.GE31027
HARVESTED ON: 12/14/2010
SEQUENCED ON: 7/21/2014
EXPECTED MW: 25kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVDPGVSPG CVRFVTLEIS PSMTMQGERL DAVVAEAVAG DRNALREVLE TIRPIVVRYC RARVGTVERS GLSADDVAQE VCLATITALP RYRDRGRPFL AFLYGIAAHK VADAHRAAGR DRAYPAETLP ERWSADAGPE QMAIEADSVT RMNELLEILP AKQREILILR VVVGLSAEET AAAVGSTTGA VRVAQHRALQ RLKDEIVAAG D
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtcg atccgggagt tagcccggga tgtgtccgct tcgtaacgtt ggagatatcg ccgtcgatga caatgcaagg ggaacgtctc gacgctgtgg ttgcggaggc cgtggcagga gaccggaacg cgcttcggga ggtgctggag accatccgcc cgatcgtcgt gcgatattgc cgagcgcgag tcggcacggt cgagcggagc ggcctgtcag cagatgacgt ggcacaggag gtgtgcttgg ccaccataac ggcgctgccg cgctatcggg accgcggccg gccattcctg gcgtttctgt acggcatcgc ggcgcacaag gttgccgacg cccatcgggc agccggccgt gaccgggcct atcccgccga aacgcttcct gagcgctggt cagccgacgc cggcccggag cagatggcca tcgaggccga ttcggtcacc cggatgaacg aattgcttga gatcttgccg gccaagcaac gcgagatcct cattctgcgt gttgtcgtcg gcctgtccgc ggaagagacc gccgccgccg tcggcagcac cacgggggcg gtccgggtgg cccaacaccg tgcacttcag cggctgaagg acgagattgt tgcggcaggt gact
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVDPGVSPG CVRFVTLEIS PSMTMQGERL DAVVAEAVAG DRNALREVLE TIRPIVVRYC RARVGTVERS GLSADDVAQE VCLATITALP RYRDRGRPFL AFLYGIAAHK VADAHRAAGR DRAYPAETLP ERWSADAGPE QMAIEADSVT RMNELLEILP AKQREILILR VVVGLSAEET AAAVGSTTGA VRVAQHRALQ RLKDEIVAAG DYA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtcgatccg ggagttagcc cgggatgtgt ccgcttcgta acgttggaga tatcgccgtc gatgacaatg caaggggaac gtctcgacgc tgtggttgcg gaggccgtgg caggagaccg gaacgcgctt cgggaggtgc tggagaccat ccgcccgatc gtcgtgcgat attgccgagc gcgagtcggc acggtcgagc ggagcggcct gtcagcagat gacgtggcac aggaggtgtg cttggccacc ataacggcgc tgccgcgcta tcgggaccgc ggccggccat tcctggcgtt tctgtacggc atcgcggcgc acaaggttgc cgacgcccat cgggcagccg gccgtgaccg ggcctatccc gccgaaacgc ttcctgagcg ctggtcagcc gacgccggcc cggagcagat ggccatcgag gccgattcgg tcacccggat gaacgaattg cttgagatct tgccggccaa gcaacgcgag atcctcattc tgcgtgttgt cgtcggcctg tccgcggaag agaccgccgc cgccgtcggc agcaccacgg gggcggtccg ggtggcccaa caccgtgcac ttcagcggct gaaggacgag attgttgcgg caggtgacta tgcgtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa