MytuD.17758.a

Putative Rieske 2Fe-2S iron-sulfur protein Rv3818/MT3926

CENTER ID: MytuD.17758.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.17758.a.A1.GE31022 Full length( MytuD.17758.a ) 1 516
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.4251
RefSeq: YP_006517315.1
RefSeq: NP_218335.1
TubercuList: Rv3818
MTB Network Portal: Rv3818
UniProt: P9WH21

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQVTSVGHAG FLIQTQAGSI LCDPWVNPAY FASWFPFPDN SGLDWGALGE CDYLYVSHLH KDHFDAENLR AHVNKDAVVL LPDFPVPDLR NELQKLGFHR FFETTDSVKH RLRGPNGDLD VMIIALRAPA DGPIGDSALV VADGETTAFN MNDARPVDLD VLASEFGHID VHMLQYSGAI WYPMVYDMPA RAKDAFGAQK RQRQMDRARQ YIAQVGATWV VPSAGPPCFL APELRHLNDD GSDPANIFPD QMVFLDQMRA HGQDGGLLMI PGSTADFTGT TLNSLRHPLP AEQVEAIFTT DKAAYIADYA DRMAPVLAAQ KAGWAAAAGE PLLQPLRTLF EPIMLQSNEI CDGIGYPVEL AIGPETIVLD FPKRAVREPI PDERFRYGFA IAPELVRTVL RDNEPDWVNT IFLSTRFRAW RVGGYNEYLY TFFKCLTDER IAYADGWFAE AHDDSSSITL NGWEIQRRCP HLKADLSKFG VVEGNTLTCN LHGWQWRLDD GRCLTARGHQ LRSSRP
NT Sequence
GTGCAGGTCA CAAGCGTTGG TCACGCCGGC TTTCTGATCC AGACCCAGGC CGGCAGCATC CTGTGCGACC CTTGGGTCAA TCCGGCCTAC TTTGCGTCTT GGTTTCCGTT CCCCGACAAC AGCGGGCTGG ACTGGGGCGC TTTGGGTGAG TGCGATTATC TGTATGTCTC GCACCTACAT AAGGACCACT TCGACGCGGA AAATCTACGA GCGCACGTCA ACAAGGACGC CGTCGTGCTG CTGCCCGACT TTCCGGTACC CGACCTGCGA AATGAGTTGC AGAAGTTAGG ATTTCATCGG TTCTTCGAAA CCACCGACTC GGTCAAACAC CGCCTGAGGG GACCCAACGG CGATCTCGAC GTGATGATCA TCGCACTGCG GGCCCCCGCC GACGGTCCGA TCGGCGACTC GGCGCTAGTC GTTGCCGACG GCGAAACAAC GGCTTTCAAC ATGAACGACG CCCGCCCGGT CGATTTGGAC GTGCTGGCAT CGGAGTTCGG TCACATCGAC GTGCATATGC TGCAGTACTC GGGCGCGATC TGGTACCCGA TGGTCTACGA CATGCCGGCG CGCGCGAAGG ATGCGTTCGG CGCCCAAAAG CGGCAACGGC AGATGGACCG TGCTCGCCAG TACATCGCGC AGGTGGGAGC GACGTGGGTG GTGCCGTCGG CGGGGCCGCC ATGCTTTTTA GCCCCCGAGC TGCGCCACCT CAACGACGAC GGTAGCGATC CGGCCAATAT CTTCCCCGAC CAGATGGTGT TCCTGGATCA GATGCGGGCG CACGGCCAGG ACGGCGGGCT GCTGATGATC CCCGGCTCGA CTGCGGATTT CACTGGTACA ACCCTGAATT CATTGCGCCA TCCACTGCCC GCCGAACAGG TCGAGGCCAT CTTTACCACC GACAAAGCCG CATACATCGC TGACTATGCC GACCGGATGG CGCCGGTGCT CGCCGCGCAA AAGGCTGGCT GGGCCGCCGC CGCCGGCGAG CCACTGCTGC AGCCGCTGCG CACCCTGTTC GAGCCGATCA TGCTGCAAAG CAACGAGATC TGCGACGGCA TCGGATACCC GGTCGAGCTC GCCATCGGTC CCGAAACCAT TGTTTTGGAC TTTCCGAAAA GAGCTGTACG AGAACCGATT CCCGACGAGA GGTTCCGCTA CGGGTTCGCG ATCGCGCCGG AGCTGGTGCG CACGGTGCTG CGCGACAACG AACCCGACTG GGTCAACACC ATCTTCTTAT CCACCCGATT TCGGGCATGG CGGGTTGGTG GCTACAACGA ATACCTTTAC ACGTTCTTCA AGTGTCTGAC CGACGAACGC ATCGCCTACG CCGACGGCTG GTTCGCCGAG GCCCACGATG ACTCCTCATC GATCACCCTG AACGGTTGGG AGATCCAGCG CCGCTGCCCC CATCTCAAAG CCGACCTATC GAAATTCGGT GTGGTGGAAG GCAACACGCT CACTTGTAAC CTGCACGGCT GGCAGTGGCG TCTGGACGAC GGTCGCTGCC TCACCGCCCG GGGCCATCAA CTACGCAGTT CACGGCCA
Details for MytuD.17758.a.A1.GE31022
HARVESTED ON: 12/14/2010
SEQUENCED ON: 1/13/2011
EXPECTED MW: 60kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 86
PERCENT COVERAGE: 61
Validated AA Sequence
MFXXLXXXXX XXXXXXXXSS XXGPGSMVQV TSVGHAGFLI QTQAGSILCD PWVNPAYFAS WFPFPDNSGL DWGALGECDY LYVSHLHKDH FDAENLRAHV NKDAVVLLPD FPVPDLRNEL QKLGFHRFFE TTDSVKHRLR GPNGDLDVMI IALRAPADGP IGDSALVVAD GETTAFNMND ARPVDLDVLA SEFGHIDVHM LQYSGAIWYP MVYDMPARAK DAFGAQKRQR QMDRARQYIA QVGATWVVPS XGPPCFLAPE LRHLNDDGSD PAXIFPDQMV FLDXXXXHGQ DXXLLXIPGS TAXFTGTXLN XLRXPXXAXX VXXXLXXXXR IHX
Validated NT Sequence
gcggnncagc catgtttnnn nnnctcnngn nnannnnnnn nnnannanna nnngnnnncn nnnnnagctc annccnnggt cctggttcga tggtgcaggt cacaagcgtt ggtcacgccg gctttctgat ccagacccag gccggcagca tcctgtgcga cccttgggtc aatccggcct actttgcgtc ttggtttccg ttccccgaca acagcgggct ggactggggc gctttgggtg agtgcgatta tctgtatgtc tcgcacctac ataaggacca cttcgacgcg gaaaatctac gagcgcacgt caacaaggac gccgtcgtgc tgctgcccga ctttccggta cccgacctgc gaaatgagtt gcagaagtta ggatttcatc ggttcttcga aaccaccgac tcggtcaaac accgcctgag gggacccaac ggcgatctcg acgtgatgat catcgcactg cgggcccccg ccgacggtcc gatcggcgac tcggcgctag tcgttgccga cggcgaaaca acggctttca acatgaacga cgcccgcccg gtcgatttgg acgtgctggc atcggagttc ggtcacatcg acgtgcatat gctgcagtac tcgggcgcga tctggtaccc gatggtctac gacatgccgg cgcgcgcgaa ggatgcgttc ggcgcccaaa agcggcaacg gcagatggac cgtgctcgcc agtacatcgc gcaggtggga gcgacgtggg tggtgccgtc ggnggggccg ccatgctttt tagcccccga gctgcgncac ctcaacgacg acggtagcga tccggccnat atcttccccg accagatggt gttcctggat canangnnnn cncacggnca ggacgncngn ctgctganga tccccggctc gactgcnnat ttcactggta canccctgaa ttnnttgcgc cntccnnngn ccgccganan ggtcgannnn nctttacnnc nncnaanccg catacatcnn tgantatgcc nnncnnnnnn nnnnncncnn cncgnaaaag nnnnnngggn cnnnnnnnnn ncnannnnng cnnnannnnn nnnnnnnncn nnnnnnnnnn annannnnnn naannnnnnn nnnnngnnan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc nnnnnnnnnt nnnntnnnnn naaaana
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVQVTSVGH AGFLIQTQAG SILCDPWVNP AYFASWFPFP DNSGLDWGAL GECDYLYVSH LHKDHFDAEN LRAHVNKDAV VLLPDFPVPD LRNELQKLGF HRFFETTDSV KHRLRGPNGD LDVMIIALRA PADGPIGDSA LVVADGETTA FNMNDARPVD LDVLASEFGH IDVHMLQYSG AIWYPMVYDM PARAKDAFGA QKRQRQMDRA RQYIAQVGAT WVVPSAGPPC FLAPELRHLN DDGSDPANIF PDQMVFLDQM RAHGQDGGLL MIPGSTADFT GTTLNSLRHP LPAEQVEAIF TTDKAAYIAD YADRMAPVLA AQKAGWAAAA GEPLLQPLRT LFEPIMLQSN EICDGIGYPV ELAIGPETIV LDFPKRAVRE PIPDERFRYG FAIAPELVRT VLRDNEPDWV NTIFLSTRFR AWRVGGYNEY LYTFFKCLTD ERIAYADGWF AEAHDDSSSI TLNGWEIQRR CPHLKADLSK FGVVEGNTLT CNLHGWQWRL DDGRCLTARG HQLRSSRP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcaggtc acaagcgttg gtcacgccgg ctttctgatc cagacccagg ccggcagcat cctgtgcgac ccttgggtca atccggccta ctttgcgtct tggtttccgt tccccgacaa cagcgggctg gactggggcg ctttgggtga gtgcgattat ctgtatgtct cgcacctaca taaggaccac ttcgacgcgg aaaatctacg agcgcacgtc aacaaggacg ccgtcgtgct gctgcccgac tttccggtac ccgacctgcg aaatgagttg cagaagttag gatttcatcg gttcttcgaa accaccgact cggtcaaaca ccgcctgagg ggacccaacg gcgatctcga cgtgatgatc atcgcactgc gggcccccgc cgacggtccg atcggcgact cggcgctagt cgttgccgac ggcgaaacaa cggctttcaa catgaacgac gcccgcccgg tcgatttgga cgtgctggca tcggagttcg gtcacatcga cgtgcatatg ctgcagtact cgggcgcgat ctggtacccg atggtctacg acatgccggc gcgcgcgaag gatgcgttcg gcgcccaaaa gcggcaacgg cagatggacc gtgctcgcca gtacatcgcg caggtgggag cgacgtgggt ggtgccgtcg gcggggccgc catgcttttt agcccccgag ctgcgccacc tcaacgacga cggtagcgat ccggccaata tcttccccga ccagatggtg ttcctggatc agatgcgggc gcacggccag gacggcgggc tgctgatgat ccccggctcg actgcggatt tcactggtac aaccctgaat tcattgcgcc atccactgcc cgccgaacag gtcgaggcca tctttaccac cgacaaagcc gcatacatcg ctgactatgc cgaccggatg gcgccggtgc tcgccgcgca aaaggctggc tgggccgccg ccgccggcga gccactgctg cagccgctgc gcaccctgtt cgagccgatc atgctgcaaa gcaacgagat ctgcgacggc atcggatacc cggtcgagct cgccatcggt cccgaaacca ttgttttgga ctttccgaaa agagctgtac gagaaccgat tcccgacgag aggttccgct acgggttcgc gatcgcgccg gagctggtgc gcacggtgct gcgcgacaac gaacccgact gggtcaacac catcttctta tccacccgat ttcgggcatg gcgggttggt ggctacaacg aataccttta cacgttcttc aagtgtctga ccgacgaacg catcgcctac gccgacggct ggttcgccga ggcccacgat gactcctcat cgatcaccct gaacggttgg gagatccagc gccgctgccc ccatctcaaa gccgacctat cgaaattcgg tgtggtggaa ggcaacacgc tcacttgtaa cctgcacggc tggcagtggc gtctggacga cggtcgctgc ctcaccgccc ggggccatca actacgcagt tcacggccat aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa