MytuD.17729.a

Putative uncharacterized protein

CENTER ID: MytuD.17729.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.17729.a.A1.GE31019 Full length( MytuD.17729.a ) 1 411
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.85
RefSeq: NP_214588.1
TubercuList: Rv0074
MTB Network Portal: Rv0074
UniProt: O53619

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGDLSISQVS ARPGRIGIRA RQMFDGYRFQ RGPVLVVVED GRISAVDFAG SACPDMNLVD LGESTLLPGL VDAHAHLCWD PDGRPEDLAG DPHAVLVGRA RRHAAAALRS GITTIRDLGD RDYAALALRE EYRQKTTVGP ELVVSGPPLT RSGGHCWFLG GVADSVEELV DAVQERAARG ADWIKVMATG GFVTTASDPW QPQYGSGQLA AVVAAAEQVG LPVTAHAHAT AGIAAAVAAG VDGIEHCTFL SEGSAAASPD VVEAIVAQGV WCGMTIPRVY PEMPENLVAV VQDGWRNIRR LIDAGARVAL STDAGVAPGR RHDVLPDDLV YLSRHGFTST EVLTGATAAA AASCGLGHRK GRIAPGYDAD LLAVAAGVDH DPAGLCDVKA VWRSGTQVPL QASAVGYNTP S
NT Sequence
ATGGGCGATC TGAGCATTAG CCAGGTGTCG GCGCGTCCGG GACGGATCGG GATTCGCGCT AGGCAAATGT TCGACGGATA CCGGTTTCAG CGTGGTCCCG TGCTGGTCGT GGTCGAGGAT GGTCGGATCA GCGCGGTCGA TTTTGCTGGC TCCGCCTGCC CCGATATGAA CCTGGTTGAT CTGGGTGAAT CGACTTTGTT GCCGGGTCTG GTGGATGCGC ATGCGCATTT GTGCTGGGAC CCCGACGGTA GGCCAGAGGA TTTGGCCGGC GACCCCCATG CGGTGCTGGT GGGACGGGCG CGACGGCACG CCGCGGCCGC GTTGCGCTCC GGGATCACCA CGATTCGCGA TCTCGGCGAC CGTGACTATG CGGCCTTGGC GCTGCGGGAG GAGTATCGGC AGAAAACGAC GGTGGGGCCG GAACTGGTGG TTTCTGGGCC ACCATTGACT CGCAGCGGCG GGCATTGCTG GTTCCTCGGC GGCGTGGCCG ATAGCGTCGA GGAGCTGGTT GATGCGGTGC AGGAGCGGGC CGCGCGGGGA GCGGATTGGA TCAAGGTGAT GGCCACGGGC GGATTCGTTA CCACAGCATC CGATCCGTGG CAGCCGCAGT ACGGCAGCGG CCAACTGGCC GCGGTGGTGG CGGCCGCCGA GCAGGTAGGT CTACCGGTGA CCGCACATGC ACATGCCACC GCAGGGATCG CCGCGGCGGT CGCCGCGGGT GTTGACGGCA TCGAGCACTG CACGTTCTTG AGCGAAGGCA GCGCCGCCGC CAGCCCGGAT GTTGTTGAAG CGATTGTTGC CCAAGGTGTG TGGTGCGGTA TGACGATTCC CCGGGTGTAT CCGGAGATGC CGGAGAACCT TGTCGCGGTT GTGCAGGATG GATGGCGAAA CATCCGCCGG CTCATCGACG CCGGTGCGCG TGTCGCCCTG TCCACCGACG CTGGAGTCGC CCCGGGCAGA CGCCATGACG TGCTCCCCGA CGATTTGGTG TATCTGTCTC GACACGGGTT CACCAGCACA GAGGTGCTGA CCGGCGCCAC CGCAGCGGCC GCTGCCAGCT GTGGGCTCGG CCACCGCAAG GGTCGCATCG CGCCGGGCTA CGACGCTGAT CTGCTGGCTG TTGCGGCAGG TGTGGACCAT GACCCCGCCG GACTCTGCGA CGTCAAAGCC GTCTGGCGCA GCGGAACCCA GGTACCGCTA CAAGCATCCG CTGTGGGCTA CAACACCCCG TCA
Details for MytuD.17729.a.A1.GE31019
HARVESTED ON: 12/14/2010
SEQUENCED ON: 1/13/2011
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 75
PERCENT COVERAGE: 84
Validated AA Sequence
MGTLEAQTQG PGSMGDLSIS QVSARPGRIG IRARQMFDGY RFQRGPVLVV VEDGRISAVD FAGSACPDMN LVDLGESTLL PGLVDAHAHL CWDPDGRPED LAGDPHAVLV GRARRHAAAA LRSGITTIRD LGDRDYAALA LREEYRQKTT VGPELVVSGP PLTRSGGHCW FLGGVADSVE ELVDAVQERA ARXADWIKVM ATGGFVTTAS DPWQPQYGSG QLAAVVAAXE QVGLPVTAHA HATAGIAAAV AAGVXGIEHC TFLSEXSAAA XPXVVEAIXA XXXXVRYDXS PGXXXXAXXX CXXXXXGXXX XXSXXXXXXP XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXX
Validated NT Sequence
cnnnnnnnnc ngnnnnnnnn nngcgnnnnn nnnnnnngta ccntgnntna tcaccatcac catcatatgg gtaccctgga agctcagacc cagggtcctg gttcgatggg cgatctgagc attagccagg tgtcggcgcg tccgggacgg atcgggattc gcgctaggca aatgttcgac ggataccggt ttcagcgtgg tcccgtgctg gtcgtggtcg aggatggtcg gatcagcgcg gtcgattttg ctggctccgc ctgccccgat atgaacctgg ttgatctggg tgaatcgact ttgttgccgg gtctggtgga tgcgcatgcg catttgtgct gggaccccga cggtaggcca gaggatttgg ccggcgaccc ccatgcggtg ctggtgggac gggcgcgacg gcacgccgcg gccgcgttgc gctccgggat caccacgatt cgcgatctcg gcgaccgtga ctatgcggcc ttggcgctgc gggaggagta tcggcagaaa acgacggtgg ggccggaact ggtggtttct gggccaccat tgactcgcag cggcgggcat tgctggttcc tcggcggcgt ggccgatagc gtcgaggagc tggttgatgc ggtgcaggag cgggccgcgc gggnagcgga ttggatcaag gtgatggcca cgggcggatt cgttaccaca gcatccgatc cgtggcagcc gcagtacggc agcggccaac tggccgcggt ggtggcggcc gncgagcagg taggtctacc ggtgaccgca catgcacatg ccaccgcagg gatcgccgcg gcggtcgccg cgggtgtnga nggcatcgag cactgcacgt tcttgagcga annnagcgcc gccgccngcc cggangttgt tgaagcgatt gntgcccnan gnngtnnggt gcggtatgac nattccccng ggtgnatnnn ngangccnna naannttgtn ncnntnntnn nnnnggnnaa anntnnnnnn catcnncnnn nnnnnnnngt cnncctnctn nannnnnccn ggnnnnnnnn ntgnnnngnt nnnnncnann nnnnnnnnnn nnnnnnncnn nnnnnnnnnn nnnnnnnnnn gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGDLSISQV SARPGRIGIR ARQMFDGYRF QRGPVLVVVE DGRISAVDFA GSACPDMNLV DLGESTLLPG LVDAHAHLCW DPDGRPEDLA GDPHAVLVGR ARRHAAAALR SGITTIRDLG DRDYAALALR EEYRQKTTVG PELVVSGPPL TRSGGHCWFL GGVADSVEEL VDAVQERAAR GADWIKVMAT GGFVTTASDP WQPQYGSGQL AAVVAAAEQV GLPVTAHAHA TAGIAAAVAA GVDGIEHCTF LSEGSAAASP DVVEAIVAQG VWCGMTIPRV YPEMPENLVA VVQDGWRNIR RLIDAGARVA LSTDAGVAPG RRHDVLPDDL VYLSRHGFTS TEVLTGATAA AAASCGLGHR KGRIAPGYDA DLLAVAAGVD HDPAGLCDVK AVWRSGTQVP LQASAVGYNT PS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggcgatctg agcattagcc aggtgtcggc gcgtccggga cggatcggga ttcgcgctag gcaaatgttc gacggatacc ggtttcagcg tggtcccgtg ctggtcgtgg tcgaggatgg tcggatcagc gcggtcgatt ttgctggctc cgcctgcccc gatatgaacc tggttgatct gggtgaatcg actttgttgc cgggtctggt ggatgcgcat gcgcatttgt gctgggaccc cgacggtagg ccagaggatt tggccggcga cccccatgcg gtgctggtgg gacgggcgcg acggcacgcc gcggccgcgt tgcgctccgg gatcaccacg attcgcgatc tcggcgaccg tgactatgcg gccttggcgc tgcgggagga gtatcggcag aaaacgacgg tggggccgga actggtggtt tctgggccac cattgactcg cagcggcggg cattgctggt tcctcggcgg cgtggccgat agcgtcgagg agctggttga tgcggtgcag gagcgggccg cgcggggagc ggattggatc aaggtgatgg ccacgggcgg attcgttacc acagcatccg atccgtggca gccgcagtac ggcagcggcc aactggccgc ggtggtggcg gccgccgagc aggtaggtct accggtgacc gcacatgcac atgccaccgc agggatcgcc gcggcggtcg ccgcgggtgt tgacggcatc gagcactgca cgttcttgag cgaaggcagc gccgccgcca gcccggatgt tgttgaagcg attgttgccc aaggtgtgtg gtgcggtatg acgattcccc gggtgtatcc ggagatgccg gagaaccttg tcgcggttgt gcaggatgga tggcgaaaca tccgccggct catcgacgcc ggtgcgcgtg tcgccctgtc caccgacgct ggagtcgccc cgggcagacg ccatgacgtg ctccccgacg atttggtgta tctgtctcga cacgggttca ccagcacaga ggtgctgacc ggcgccaccg cagcggccgc tgccagctgt gggctcggcc accgcaaggg tcgcatcgcg ccgggctacg acgctgatct gctggctgtt gcggcaggtg tggaccatga ccccgccgga ctctgcgacg tcaaagccgt ctggcgcagc ggaacccagg taccgctaca agcatccgct gtgggctaca acaccccgtc ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa