SSGCID: Target Page

MytuD.17724.a

Putative uncharacterized protein

CENTER ID:	MytuD.17724.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.17724.a.A1.GE31018	Full length( MytuD.17724.a )		1	229

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|83332.12.peg.4195
RefSeq:	NP_218283.1
TubercuList:	Rv3766
MTB Network Portal:	Rv3766
UniProt:	O69731

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRSAFDSGRL TFGIVYTYAR PNWWANANTV RSMIDAAGGL HPRVALMLDV ESGGNPPGDG SSWINRLYWN LADYAGSPVR IIGYANAYDF FNMWRVRPAG LRVIGAGYGS NPNLPGQVAH QYTDGSGYSP NLPQGAPPFG RCDMNSANGL TPQQFAAACG VTTTGGPLMA LTDEEQTELL TKVREIWDQL RGPNGAGWPQ LGQNEQGQDL TPVDAIAVIK NDVAAMLAE

NT Sequence

ATGCGCTCGG CATTCGACAG CGGGCGACTC ACATTCGGAA TCGTCTACAC CTACGCCCGT CCGAATTGGT GGGCCAATGC CAACACCGTG CGCTCGATGA TCGACGCAGC GGGCGGCTTG CATCCCCGGG TCGCGCTGAT GCTGGATGTC GAATCAGGCG GGAACCCGCC CGGTGACGGG TCGAGCTGGA TCAACCGGCT GTACTGGAAC CTGGCAGACT ACGCCGGCTC GCCCGTGCGA ATCATCGGTT ATGCCAACGC CTACGACTTC TTCAACATGT GGCGTGTTCG CCCGGCGGGC CTGCGCGTCA TTGGCGCGGG TTATGGTTCC AATCCGAACC TTCCCGGACA AGTGGCGCAC CAGTACACCG ACGGCAGTGG GTATAGCCCC AATCTTCCAC AGGGCGCTCC ACCGTTCGGT CGATGCGATA TGAACTCTGC CAACGGACTA ACACCGCAAC AGTTTGCCGC CGCATGCGGC GTCACAACGA CCGGAGGACC GCTGATGGCA CTCACCGACG AAGAACAAAC CGAACTACTG ACCAAAGTCC GCGAGATATG GGACCAACTG CGCGGGCCCA ACGGCGCCGG GTGGCCTCAG CTCGGACAGA ACGAACAGGG CCAGGACCTC ACTCCGGTTG ACGCGATAGC GGTGATCAAG AACGACGTGG CGGCCATGCT CGCGGAA

Details for MytuD.17724.a.A1.GE31018

HARVESTED ON:	12/14/2010
SEQUENCED ON:	12/20/2010
EXPECTED MW:	26kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMRSAFDSGR LTFGIVYTYA RPNWWANANT VRSMIDAAGG LHPRVALMLD VESGGNPPGD GSSWINRLYW NLADYAGSPV RIIGYANAYD FFNMWRVRPA GLRVIGAGYG SNPNLPGQVA HQYTDGSGYS PNLPQGAPPF GRCDMNSANG LTPQQFAAAC GVTTTGGPLM ALTDEEQTEL LTKVREIWDQ LRGPNGAGWP QLGQNEQGQD LTPVDAIAVI KNDVAAMLAE

Validated NT Sequence

ttgtttactt taagaaggag atataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atgcgctcgg cattcgacag cgggcgactc acattcggaa tcgtctacac ctacgcccgt ccgaattggt gggccaatgc caacaccgtg cgctcgatga tcgacgcagc gggcggcttg catccccggg tcgcgctgat gctggatgtc gaatcaggcg ggaacccgcc cggtgacggg tcgagctgga tcaaccggct gtactggaac ctggcagact acgccggctc gcccgtgcga atcatcggtt atgccaacgc ctacgacttc ttcaacatgt ggcgtgttcg cccggcgggc ctgcgcgtca ttggcgcggg ttatggttcc aatccgaacc ttcccggaca agtggcgcac cagtacaccg acggcagtgg gtatagcccc aatcttccac agggcgctcc accgttcggt cgatgcgata tgaactctgc caacggacta acaccgcaac agtttgccgc cgcatgcggc gtcacaacga ccggaggacc gctgatggca ctcaccgacg aagaacaaac cgaactactg accaaagtcc gcgagatatg ggaccaactg cgcgggccca acggcgccgg gtggcctcag ctcggacaga acgaacaggg ccaggacctc actccggttg acgcgatagc ggtgatcaag aacgacgtgg cggccatgct cgcggaataa taaacagcac gaacaagttc tgcagccaag cttctcgagg atccngctgc taacaaagcc cgaaaggaag ctganttggc tgctgncncc ncnna

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgctcggca ttcgacagcg ggcgactcac attcggaatc gtctacacct acgcccgtcc gaattggtgg gccaatgcca acaccgtgcg ctcgatgatc gacgcagcgg gcggcttgca tccccgggtc gcgctgatgc tggatgtcga atcaggcggg aacccgcccg gtgacgggtc gagctggatc aaccggctgt actggaacct ggcagactac gccggctcgc ccgtgcgaat catcggttat gccaacgcct acgacttctt caacatgtgg cgtgttcgcc cggcgggcct gcgcgtcatt ggcgcgggtt atggttccaa tccgaacctt cccggacaag tggcgcacca gtacaccgac ggcagtgggt atagccccaa tcttccacag ggcgctccac cgttcggtcg atgcgatatg aactctgcca acggactaac accgcaacag tttgccgccg catgcggcgt cacaacgacc ggaggaccgc tgatggcact caccgacgaa gaacaaaccg aactactgac caaagtccgc gagatatggg accaactgcg cgggcccaac ggcgccgggt ggcctcagct cggacagaac gaacagggcc aggacctcac tccggttgac gcgatagcgg tgatcaagaa cgacgtggcg gccatgctcg cggaataaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa