MytuD.17682.a

Universal stress protein Rv2319c/MT2382

CENTER ID: MytuD.17682.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.17682.a.A1.GE31012 Full length( MytuD.17682.a ) 1 292
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.2589
RefSeq: NP_216835.1
TubercuList: Rv2319c
MTB Network Portal: Rv2319c
UniProt: P9WLB5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTIVVGYLAG KVGPSALHLA VRVARMHKTS LTVATIVRRH WPTPSLARVD AEYELWSEQL AAASAREAQR YLRRLADGIE VSYHHRAHRS VSAGLLDVVE ELEAEVLVLG SFPSGRRARV LIGSTADRLL HSSPVPVAIT PRRYRCYTDR LTRLSCGYSA TSGSVDVVRR CGHLASRYGV PMRVITFAVR GRTMYPPEVG LHAEASVLEA WAAQARELLE KLRINGVVSE DVVLQVVTGN GWAQALDAAD WQDGEILALG TSPFGDVARV FLGSWSGKII RYSPVPVLVL PG
NT Sequence
GTGACCATCG TCGTCGGGTA TTTGGCTGGC AAGGTCGGTC CGTCCGCATT ACATCTAGCC GTCCGCGTCG CGCGGATGCA CAAGACGTCG CTCACAGTGG CCACCATCGT GCGCAGGCAC TGGCCGACAC CGTCGCTCGC CCGCGTCGAC GCCGAGTACG AGCTCTGGTC TGAGCAGCTG GCTGCCGCCT CCGCGCGAGA GGCCCAGCGC TACCTGCGCA GACTGGCCGA CGGGATCGAG GTCAGCTACC ACCACCGCGC ACACCGATCG GTGTCGGCTG GTCTGCTCGA CGTCGTCGAG GAACTCGAAG CCGAGGTGCT GGTGCTGGGG TCGTTTCCCA GCGGGCGGCG CGCGCGGGTG TTGATCGGCT CGACCGCCGA CCGGCTGCTG CATTCGTCGC CGGTACCGGT GGCGATCACC CCCCGCCGCT ACCGTTGCTA CACCGACCGG TTGACACGGC TCAGCTGCGG CTACTCCGCG ACGTCGGGTT CGGTCGACGT GGTGAGGCGA TGCGGCCACC TGGCCAGCCG GTATGGCGTG CCGATGCGGG TGATCACATT CGCGGTCCGC GGCCGGACCA TGTATCCGCC CGAAGTGGGG CTGCATGCCG AGGCTTCGGT TCTGGAGGCA TGGGCGGCGC AAGCGCGAGA ACTGTTGGAA AAACTAAGAA TTAACGGCGT CGTGAGTGAA GACGTTGTGC TGCAAGTGGT CACCGGGAAC GGCTGGGCGC AGGCGCTGGA CGCTGCGGAC TGGCAGGACG GGGAGATCCT GGCCTTGGGC ACCTCGCCGT TCGGTGATGT CGCGAGAGTA TTCCTCGGCT CCTGGAGTGG CAAGATCATT CGCTACAGCC CGGTCCCCGT GCTAGTACTA CCGGGC
Details for MytuD.17682.a.A1.GE31012
HARVESTED ON: 12/14/2010
SEQUENCED ON: 12/20/2010
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 85
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTIVVGYL AGKVGPSALH LAVRVARMHK TSLTVATIVR RHWPTPSLAR VDAEYELWSE QLAAASAREA QRYLRRLADG IEVSYHHRAH RSVSAGLLDV VEELEAEVLV LGSFPSGRRA RVLIGSTADR LLHSSPVPVA ITPRRYRCYT DRLTRLSCGY SATSGSVDVV RRCGHLASRY GVPMRVITFA VRGRTMYPPE VGLHAEASVL EAWAAXAREL LEKLRINGVV SEDVVLQVVT GNGWAXX
Validated NT Sequence
atttnnttta ctttaagaag gagnnatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga ccatcgtcgt cgggtatttg gctggcaagg tcggtccgtc cgcattacat ctagccgtcc gcgtcgcgcg gatgcacaag acgtcgctca cagtggccac catcgtgcgc aggcactggc cgacaccgtc gctcgcccgc gtcgacgccg agtacgagct ctggtctgag cagctggctg ccgcctccgc gcgagaggcc cagcgctacc tgcgcagact ggccgacggg atcgaggtca gctaccacca ccgcgcacac cgatcggtgt cggctggtct gctcgacgtc gtcgaggaac tcgaagccga ggtgctggtg ctggggtcgt ttcccagcgg gcggcgcgcg cgggtgttga tcggctcgac cgccgaccgg ctgctgcatt cgtcgccggt accggtggcg atcacccccc gccgctaccg ttgctacacc gaccggttga cacggctcag ctgcggctac tccgcgacgt cgggttcggt cgacgtggtg aggcgatgcg gccacctggc cagccggtat ggcgtgccga tgcgggtgat cacattcgcg gtccgcggcc ggaccatgta tccgcccgaa gtggggctgc atgccgaggc ttcggttctg gaggcatggg cggcgcnngc gcgagaactg ttggaaaaac taagaattaa cggcgtcgtg agtgaagacg ttgtgctgca agtggtcacc gggaacggct gggcgcnnng ctgacgctgc ggactggcag gacggggaga tcctggnctt gggcacctcg ncgttcggtg angtcgcgag antattncnt cngctccngg nntggcaaga tcattcncta cagcccggtc cccnnncnan nnnnnnnngg nnnannnaan annnnaannn nttntnnnnn nnannntnnn nnnnnnnnnn gntgnnnnnn aaannnnnng nangnnnnnn ng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTIVVGYL AGKVGPSALH LAVRVARMHK TSLTVATIVR RHWPTPSLAR VDAEYELWSE QLAAASAREA QRYLRRLADG IEVSYHHRAH RSVSAGLLDV VEELEAEVLV LGSFPSGRRA RVLIGSTADR LLHSSPVPVA ITPRRYRCYT DRLTRLSCGY SATSGSVDVV RRCGHLASRY GVPMRVITFA VRGRTMYPPE VGLHAEASVL EAWAAQAREL LEKLRINGVV SEDVVLQVVT GNGWAQALDA ADWQDGEILA LGTSPFGDVA RVFLGSWSGK IIRYSPVPVL VLPG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaccatc gtcgtcgggt atttggctgg caaggtcggt ccgtccgcat tacatctagc cgtccgcgtc gcgcggatgc acaagacgtc gctcacagtg gccaccatcg tgcgcaggca ctggccgaca ccgtcgctcg cccgcgtcga cgccgagtac gagctctggt ctgagcagct ggctgccgcc tccgcgcgag aggcccagcg ctacctgcgc agactggccg acgggatcga ggtcagctac caccaccgcg cacaccgatc ggtgtcggct ggtctgctcg acgtcgtcga ggaactcgaa gccgaggtgc tggtgctggg gtcgtttccc agcgggcggc gcgcgcgggt gttgatcggc tcgaccgccg accggctgct gcattcgtcg ccggtaccgg tggcgatcac cccccgccgc taccgttgct acaccgaccg gttgacacgg ctcagctgcg gctactccgc gacgtcgggt tcggtcgacg tggtgaggcg atgcggccac ctggccagcc ggtatggcgt gccgatgcgg gtgatcacat tcgcggtccg cggccggacc atgtatccgc ccgaagtggg gctgcatgcc gaggcttcgg ttctggaggc atgggcggcg caagcgcgag aactgttgga aaaactaaga attaacggcg tcgtgagtga agacgttgtg ctgcaagtgg tcaccgggaa cggctgggcg caggcgctgg acgctgcgga ctggcaggac ggggagatcc tggccttggg cacctcgccg ttcggtgatg tcgcgagagt attcctcggc tcctggagtg gcaagatcat tcgctacagc ccggtccccg tgctagtact accgggctaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa