MytuD.17655.a

POSSIBLE HYDROLASE

CENTER ID: MytuD.17655.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.17655.a.A1.GE31007 Full length( MytuD.17655.a ) 1 603
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.3433
RefSeq: YP_177923.1
TubercuList: Rv3077
MTB Network Portal: Rv3077
UniProt: Q6MX15

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MANRPDIIIV MTDEERAVPP YESAEVLAWR QRSLTGRRWF DEHGISFTRH YTGSLACVPS RPTIFTGQYP DLHGVTQTDG IGKRFDDSRL RWLRAGEVPT LGNWFRAAGY DTHYDGKWHI SHADLEDPAT GAPLATNDNE GVVDSAAVRR YLDADPLGPY GFSGWVGPEP HGAGLANSGF RRDPLVADRV VAWLTERYAR RRAGDTAAMR PFLLVASFVN PHDIVLFPAW VWRSPLKPSP LDPPHVPAAP TADEDLSTKP AAQVAYREAY YSGYGLTRMV SRNYARNAQR YRDLYYRLHA EVDGPIDRVG RAVTEGGSED AMLVRTSDHG DLLGAHGGLH QKWFNLYDEA TRVPFVIARI GEKATQPRTV SAPTSHVDLV PTLLSAAGVD VDVVAAALAE SFSEVHPLPG RDLMPVVDGA SADEGRAIYL MTRDNVLEGD TGASLLSRQL GRIVNPPAPL RIKVPAHVAA NFEGLVVRVD DTDAAGGAGH LWKLVRTFDD PATWTEPGVR HLATNGMGGD AYRTDPLDDQ WELYDLTADP IEAYNRWTDP QLHELRQHLR MLLKQQRAVS VPERNQPWPY AHRLPPSGAS NGLVRRVLGR FVR
NT Sequence
ATGGCTAACC GTCCCGACAT CATCATCGTG ATGACCGACG AGGAACGTGC GGTGCCGCCG TACGAGTCGG CCGAGGTGCT CGCCTGGCGT CAACGCAGCT TGACCGGCCG CCGTTGGTTC GACGAGCACG GGATCAGTTT CACTCGGCAC TACACCGGTT CGCTGGCGTG CGTGCCCAGC CGCCCGACGA TTTTCACCGG CCAATATCCG GATCTGCACG GCGTCACCCA GACCGACGGC ATCGGCAAGC GATTCGATGA TTCGCGGCTG CGCTGGCTAC GGGCCGGCGA GGTGCCGACG TTGGGTAACT GGTTTCGCGC GGCCGGGTAT GACACTCACT ACGACGGCAA GTGGCACATC TCGCACGCCG ATCTGGAAGA CCCCGCGACC GGTGCACCAC TGGCCACCAA CGACAACGAG GGCGTCGTCG ACTCGGCCGC GGTGCGGCGT TACCTCGACG CCGACCCGCT CGGGCCATAC GGCTTCTCCG GGTGGGTGGG CCCCGAGCCC CATGGGGCGG GGTTGGCCAA CAGCGGTTTT CGTCGCGACC CGCTGGTCGC CGATCGTGTC GTCGCGTGGC TGACCGAGCG CTACGCCCGG CGGCGCGCCG GTGACACCGC CGCGATGCGC CCGTTCTTGC TGGTGGCCAG CTTCGTCAAC CCGCACGACA TCGTGCTGTT CCCGGCATGG GTGTGGCGCA GCCCGCTAAA GCCCTCCCCA CTGGACCCGC CACACGTACC GGCGGCGCCG ACCGCCGACG AGGACCTGTC GACCAAGCCG GCCGCGCAGG TCGCCTACCG GGAGGCGTAC TACTCCGGAT ACGGCCTAAC GCGTATGGTC AGCCGCAACT ATGCCCGCAA CGCGCAGCGC TACCGGGACC TCTACTACCG CCTGCACGCC GAGGTCGACG GGCCGATCGA CCGTGTGGGC CGCGCGGTCA CCGAGGGCGG ATCCGAGGAT GCCATGCTGG TGCGCACCTC CGACCATGGC GATCTGCTCG GAGCGCATGG CGGACTGCAC CAGAAGTGGT TCAACCTCTA TGACGAGGCA ACCAGGGTGC CGTTCGTCAT TGCCCGCATC GGCGAGAAGG CAACCCAACC GCGCACGGTC TCGGCGCCCA CCTCGCATGT CGACTTGGTG CCGACGCTGC TTAGCGCGGC CGGCGTGGAC GTAGACGTGG TGGCCGCGGC CCTGGCCGAA TCGTTCTCCG AGGTGCATCC GCTGCCCGGT CGTGACCTGA TGCCGGTCGT GGACGGGGCT TCGGCCGACG AGGGTCGGGC CATCTACCTG ATGACGCGTG ACAACGTGCT CGAAGGCGAC ACCGGCGCGT CCCTGCTGTC GCGGCAACTG GGCCGTATCG TGAATCCGCC TGCACCGCTG CGCATCAAGG TGCCCGCCCA CGTCGCCGCC AACTTCGAGG GATTAGTCGT ACGGGTCGAT GACACCGACG CCGCCGGTGG TGCCGGGCAC CTGTGGAAAC TGGTGCGTAC CTTCGACGAC CCGGCCACCT GGACCGAACC CGGTGTGCGT CACCTGGCCA CCAACGGCAT GGGCGGCGAC GCCTATCGCA CCGATCCACT GGACGACCAG TGGGAGCTCT ACGACCTGAC CGCCGATCCC ATCGAGGCAT ACAACCGGTG GACCGACCCA CAACTGCACG AGCTGCGACA GCATCTGCGG ATGCTGCTCA AACAGCAACG TGCGGTATCG GTACCGGAAC GCAACCAACC GTGGCCGTAT GCTCATCGAC TGCCGCCGAG CGGGGCATCC AACGGTTTGG TGCGGCGAGT GTTGGGAAGG TTCGTGCGC
Details for MytuD.17655.a.A1.GE31007
HARVESTED ON: 12/14/2010
SEQUENCED ON: 12/20/2010
EXPECTED MW: 69kDa
OBSERVED MW: 70kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 60
PERCENT COVERAGE: 8
Validated AA Sequence
MGTLEAQTQG PGSMXXXLNX NKQMTDEERA XPPXXXXXXL AWXXRXLXGR
Validated NT Sequence
tnnnnnnnng nnnngtnnnn nnnnnnnann nnnntcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgnnnn accnnctcaa cannaacaag cagatgaccg acgaggaacg tgcggngccg ccgnnnnann nngncgannn gctcgcctgg ngtcnacgcn gcttgncngg ncgctgancn nnanctnnna annnantttc ncnnggnann ngngcnnnta nanannncna anaanngctg ngcnnnnnnn ntcgngggnn cnnnnntgcn nanaaaccca aangannang agangngnnn ngnncccncn nngnnnnann nnnnanaann tnannnncng nnnngcnagt ngnnnncnnn nnnntgnnnn nnngnncnnn ntnnntnnnc nnnnnnnnnn nnnnnnncnn nnnnntgatg ntnnntnnnn nntnnnnacn nnnnnnnnnn nncnnanntn gntgnctnan nnntnncnnn nnnnnnnntn nnnnnnnnnn c
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMANRPDIII VMTDEERAVP PYESAEVLAW RQRSLTGRRW FDEHGISFTR HYTGSLACVP SRPTIFTGQY PDLHGVTQTD GIGKRFDDSR LRWLRAGEVP TLGNWFRAAG YDTHYDGKWH ISHADLEDPA TGAPLATNDN EGVVDSAAVR RYLDADPLGP YGFSGWVGPE PHGAGLANSG FRRDPLVADR VVAWLTERYA RRRAGDTAAM RPFLLVASFV NPHDIVLFPA WVWRSPLKPS PLDPPHVPAA PTADEDLSTK PAAQVAYREA YYSGYGLTRM VSRNYARNAQ RYRDLYYRLH AEVDGPIDRV GRAVTEGGSE DAMLVRTSDH GDLLGAHGGL HQKWFNLYDE ATRVPFVIAR IGEKATQPRT VSAPTSHVDL VPTLLSAAGV DVDVVAAALA ESFSEVHPLP GRDLMPVVDG ASADEGRAIY LMTRDNVLEG DTGASLLSRQ LGRIVNPPAP LRIKVPAHVA ANFEGLVVRV DDTDAAGGAG HLWKLVRTFD DPATWTEPGV RHLATNGMGG DAYRTDPLDD QWELYDLTAD PIEAYNRWTD PQLHELRQHL RMLLKQQRAV SVPERNQPWP YAHRLPPSGA SNGLVRRVLG RFVR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctaaccgt cccgacatca tcatcgtgat gaccgacgag gaacgtgcgg tgccgccgta cgagtcggcc gaggtgctcg cctggcgtca acgcagcttg accggccgcc gttggttcga cgagcacggg atcagtttca ctcggcacta caccggttcg ctggcgtgcg tgcccagccg cccgacgatt ttcaccggcc aatatccgga tctgcacggc gtcacccaga ccgacggcat cggcaagcga ttcgatgatt cgcggctgcg ctggctacgg gccggcgagg tgccgacgtt gggtaactgg tttcgcgcgg ccgggtatga cactcactac gacggcaagt ggcacatctc gcacgccgat ctggaagacc ccgcgaccgg tgcaccactg gccaccaacg acaacgaggg cgtcgtcgac tcggccgcgg tgcggcgtta cctcgacgcc gacccgctcg ggccatacgg cttctccggg tgggtgggcc ccgagcccca tggggcgggg ttggccaaca gcggttttcg tcgcgacccg ctggtcgccg atcgtgtcgt cgcgtggctg accgagcgct acgcccggcg gcgcgccggt gacaccgccg cgatgcgccc gttcttgctg gtggccagct tcgtcaaccc gcacgacatc gtgctgttcc cggcatgggt gtggcgcagc ccgctaaagc cctccccact ggacccgcca cacgtaccgg cggcgccgac cgccgacgag gacctgtcga ccaagccggc cgcgcaggtc gcctaccggg aggcgtacta ctccggatac ggcctaacgc gtatggtcag ccgcaactat gcccgcaacg cgcagcgcta ccgggacctc tactaccgcc tgcacgccga ggtcgacggg ccgatcgacc gtgtgggccg cgcggtcacc gagggcggat ccgaggatgc catgctggtg cgcacctccg accatggcga tctgctcgga gcgcatggcg gactgcacca gaagtggttc aacctctatg acgaggcaac cagggtgccg ttcgtcattg cccgcatcgg cgagaaggca acccaaccgc gcacggtctc ggcgcccacc tcgcatgtcg acttggtgcc gacgctgctt agcgcggccg gcgtggacgt agacgtggtg gccgcggccc tggccgaatc gttctccgag gtgcatccgc tgcccggtcg tgacctgatg ccggtcgtgg acggggcttc ggccgacgag ggtcgggcca tctacctgat gacgcgtgac aacgtgctcg aaggcgacac cggcgcgtcc ctgctgtcgc ggcaactggg ccgtatcgtg aatccgcctg caccgctgcg catcaaggtg cccgcccacg tcgccgccaa cttcgaggga ttagtcgtac gggtcgatga caccgacgcc gccggtggtg ccgggcacct gtggaaactg gtgcgtacct tcgacgaccc ggccacctgg accgaacccg gtgtgcgtca cctggccacc aacggcatgg gcggcgacgc ctatcgcacc gatccactgg acgaccagtg ggagctctac gacctgaccg ccgatcccat cgaggcatac aaccggtgga ccgacccaca actgcacgag ctgcgacagc atctgcggat gctgctcaaa cagcaacgtg cggtatcggt accggaacgc aaccaaccgt ggccgtatgc tcatcgactg ccgccgagcg gggcatccaa cggtttggtg cggcgagtgt tgggaaggtt cgtgcgctaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa