MytuD.17614.a

ClpP protease chaperone protein ClpC2; uncharacterized protein Rv2667/MT2741

CENTER ID: MytuD.17614.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.17614.a.A1.GE31001 Full length( MytuD.17614.a ) 1 252
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.17614.a.A1.PS00982 Full length( MytuD.17614.a ) 1 252

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.2977
RefSeq: NP_337242.1
RefSeq: YP_177897.1
TubercuList: Rv2667
MTB Network Portal: Rv2667
UniProt: P9WPC7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPEPTPTAYP VRLDELINAI KRVHSDVLDQ LSDAVLAAEH LGEIADHLIG HFVDQARRSG ASWSDIGKSM GVTKQAAQKR FVPRAEATTL DSNQGFRRFT PRARNAVVAA QNAAHGAASS EITPDHLLLG VLTDPAALAT ALLQQQEIDI ATLRTAVTLP PAVTEPPQPI PFSGPARKVL ELTFREALRL GHNYIGTEHL LLALLELEDG DGPLHRSGVD KSRAEADLIT TLASLTGANA AGATDAGATD AGX
NT Sequence
ATGCCGGAGC CCACACCCAC CGCCTACCCC GTCCGCCTCG ACGAGCTCAT CAACGCCATC AAACGGGTGC ACAGCGACGT GTTGGACCAA CTCAGCGACG CCGTCCTGGC CGCCGAGCAT CTCGGCGAAA TCGCCGATCA CTTAATCGGC CACTTCGTCG ATCAGGCCCG CCGCTCGGGC GCCTCCTGGT CCGATATCGG CAAGAGCATG GGCGTCACCA AACAGGCCGC GCAAAAGCGG TTCGTCCCCC GAGCCGAAGC CACCACACTG GATTCAAACC AGGGCTTCAG GCGTTTCACG CCGCGGGCCC GCAACGCCGT GGTCGCGGCC CAAAACGCCG CGCACGGAGC CGCCAGCAGC GAGATCACCC CCGATCACCT GTTGTTGGGA GTGCTCACTG ACCCGGCCGC ACTGGCCACG GCGTTGCTTC AGCAGCAGGA GATCGACATC GCAACCCTGC GTACGGCGGT CACGCTCCCC CCGGCAGTCA CCGAGCCGCC TCAGCCGATC CCGTTCAGCG GCCCGGCGCG CAAGGTCCTC GAGCTCACCT TCCGCGAGGC GCTTCGGCTG GGCCACAACT ACATCGGGAC CGAACACCTG CTGCTGGCAC TGCTAGAACT CGAGGACGGG GATGGGCCGT TGCATCGATC CGGCGTCGAC AAGAGCCGCG CCGAGGCCGA CCTGATCACC ACGCTCGCAT CGCTCACCGG CGCCAACGCT GCCGGCGCAA CCGATGCCGG CGCAACCGAT GCCGGC
Details for MytuD.17614.a.A1.GE31001
HARVESTED ON: 12/14/2010
SEQUENCED ON: 12/20/2010
EXPECTED MW: 28kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPEPTPTAY PVRLDELINA IKRVHSDVLD QLSDAVLAAE HLGEIADHLI GHFVDQARRS GASWSDIGKS MGVTKQAAQK RFVPRAEATT LDSNQGFRRF TPRARNAVVA AQNAAHGAAS SEITPDHLLL GVLTDPAALA TALLQQQEID IATLRTAVTL PPAVTEPPQP IPFSGPARKV LELTFREALR LGHNYIGTEH LLLALLELED GDGPLHRSGV DKSRAEADLI TTLASLTGAN AAGATDAG
Validated NT Sequence
ttattagccg gcatcggttg cgccggcagc gttggcgccg gtgagcgatg cgagcgtggt gatcaggtcg gcctcggcgc ggctcttgtc gacgccggat cgatgcaacg gcccatcccc gtcctcgagt tctagcagtg ccagcagcag gtgttcggtc ccgatgtagt tgtggcccag ccgaagcgcc tcgcggaagg tgagctcgag gaccttgcgc gccgggccgc tgaacgggat cggctgaggc ggctcggtga ctgccggggg gagcgtgacc gccgtacgca gggttgcgat gtcgatctcc tgctgctgaa gcaacgccgt ggccagtgcg gccgggtcag tgagcactcc caacaacagg tgatcggggg tgatctcgct gctggcggct ccgtgcgcgg cgttttgggc cgcgaccacg gcgttgcggg cccgcggcgt gaaacgcctg aagccctggt ttgaatccag tgtggtggct tcggctcggg ggacgaaccg cttttgcgcg gcctgtttgg tgacgcccat gctcttgccg atatcggacc aggaggcgcc cgagcggcgg gcctgatcga cgaagtggcc gattaagtga tcggcgattt cgccgagatg ctcggcggcc aggacggcgt cgctgagttg gtccaacacg tcgctgtgca cccgtttgat ggcgttgatg agctcgtcga ggcggacggg gtaggcggtg ggtgtgggct ccggcatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPEPTPTAY PVRLDELINA IKRVHSDVLD QLSDAVLAAE HLGEIADHLI GHFVDQARRS GASWSDIGKS MGVTKQAAQK RFVPRAEATT LDSNQGFRRF TPRARNAVVA AQNAAHGAAS SEITPDHLLL GVLTDPAALA TALLQQQEID IATLRTAVTL PPAVTEPPQP IPFSGPARKV LELTFREALR LGHNYIGTEH LLLALLELED GDGPLHRSGV DKSRAEADLI TTLASLTGAN AAGATDAGAT DAG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccggagccc acacccaccg cctaccccgt ccgcctcgac gagctcatca acgccatcaa acgggtgcac agcgacgtgt tggaccaact cagcgacgcc gtcctggccg ccgagcatct cggcgaaatc gccgatcact taatcggcca cttcgtcgat caggcccgcc gctcgggcgc ctcctggtcc gatatcggca agagcatggg cgtcaccaaa caggccgcgc aaaagcggtt cgtcccccga gccgaagcca ccacactgga ttcaaaccag ggcttcaggc gtttcacgcc gcgggcccgc aacgccgtgg tcgcggccca aaacgccgcg cacggagccg ccagcagcga gatcaccccc gatcacctgt tgttgggagt gctcactgac ccggccgcac tggccacggc gttgcttcag cagcaggaga tcgacatcgc aaccctgcgt acggcggtca cgctcccccc ggcagtcacc gagccgcctc agccgatccc gttcagcggc ccggcgcgca aggtcctcga gctcaccttc cgcgaggcgc ttcggctggg ccacaactac atcgggaccg aacacctgct gctggcactg ctagaactcg aggacgggga tgggccgttg catcgatccg gcgtcgacaa gagccgcgcc gaggccgacc tgatcaccac gctcgcatcg ctcaccggcg ccaacgctgc cggcgcaacc gatgccggcg caaccgatgc cggctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for MytuD.17614.a.A1.PS00982
PURIFICATION DATe: 2/22/2011
CONCENTRATION: 52.3mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPEPTPTAY PVRLDELINA IKRVHSDVLD QLSDAVLAAE HLGEIADHLI GHFVDQARRS GASWSDIGKS MGVTKQAAQK RFVPRAEATT LDSNQGFRRF TPRARNAVVA AQNAAHGAAS SEITPDHLLL GVLTDPAALA TALLQQQEID IATLRTAVTL PPAVTEPPQP IPFSGPARKV LELTFREALR LGHNYIGTEH LLLALLELED GDGPLHRSGV DKSRAEADLI TTLASLTGAN AAGATDAG
Validated NT Sequence
ttattagccg gcatcggttg cgccggcagc gttggcgccg gtgagcgatg cgagcgtggt gatcaggtcg gcctcggcgc ggctcttgtc gacgccggat cgatgcaacg gcccatcccc gtcctcgagt tctagcagtg ccagcagcag gtgttcggtc ccgatgtagt tgtggcccag ccgaagcgcc tcgcggaagg tgagctcgag gaccttgcgc gccgggccgc tgaacgggat cggctgaggc ggctcggtga ctgccggggg gagcgtgacc gccgtacgca gggttgcgat gtcgatctcc tgctgctgaa gcaacgccgt ggccagtgcg gccgggtcag tgagcactcc caacaacagg tgatcggggg tgatctcgct gctggcggct ccgtgcgcgg cgttttgggc cgcgaccacg gcgttgcggg cccgcggcgt gaaacgcctg aagccctggt ttgaatccag tgtggtggct tcggctcggg ggacgaaccg cttttgcgcg gcctgtttgg tgacgcccat gctcttgccg atatcggacc aggaggcgcc cgagcggcgg gcctgatcga cgaagtggcc gattaagtga tcggcgattt cgccgagatg ctcggcggcc aggacggcgt cgctgagttg gtccaacacg tcgctgtgca cccgtttgat ggcgttgatg agctcgtcga ggcggacggg gtaggcggtg ggtgtgggct ccggcatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPEPTPTAY PVRLDELINA IKRVHSDVLD QLSDAVLAAE HLGEIADHLI GHFVDQARRS GASWSDIGKS MGVTKQAAQK RFVPRAEATT LDSNQGFRRF TPRARNAVVA AQNAAHGAAS SEITPDHLLL GVLTDPAALA TALLQQQEID IATLRTAVTL PPAVTEPPQP IPFSGPARKV LELTFREALR LGHNYIGTEH LLLALLELED GDGPLHRSGV DKSRAEADLI TTLASLTGAN AAGATDAGAT DAG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCGGAGCCC ACACCCACCG CCTACCCCGT CCGCCTCGAC GAGCTCATCA ACGCCATCAA ACGGGTGCAC AGCGACGTGT TGGACCAACT CAGCGACGCC GTCCTGGCCG CCGAGCATCT CGGCGAAATC GCCGATCACT TAATCGGCCA CTTCGTCGAT CAGGCCCGCC GCTCGGGCGC CTCCTGGTCC GATATCGGCA AGAGCATGGG CGTCACCAAA CAGGCCGCGC AAAAGCGGTT CGTCCCCCGA GCCGAAGCCA CCACACTGGA TTCAAACCAG GGCTTCAGGC GTTTCACGCC GCGGGCCCGC AACGCCGTGG TCGCGGCCCA AAACGCCGCG CACGGAGCCG CCAGCAGCGA GATCACCCCC GATCACCTGT TGTTGGGAGT GCTCACTGAC CCGGCCGCAC TGGCCACGGC GTTGCTTCAG CAGCAGGAGA TCGACATCGC AACCCTGCGT ACGGCGGTCA CGCTCCCCCC GGCAGTCACC GAGCCGCCTC AGCCGATCCC GTTCAGCGGC CCGGCGCGCA AGGTCCTCGA GCTCACCTTC CGCGAGGCGC TTCGGCTGGG CCACAACTAC ATCGGGACCG AACACCTGCT GCTGGCACTG CTAGAACTCG AGGACGGGGA TGGGCCGTTG CATCGATCCG GCGTCGACAA GAGCCGCGCC GAGGCCGACC TGATCACCAC GCTCGCATCG CTCACCGGCG CCAACGCTGC CGGCGCAACC GATGCCGGCG CAACCGATGC CGGCTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA