SSGCID: Target Page

MytuD.17600.a

PROBABLE TRANSPOSASE

CENTER ID:	MytuD.17600.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.17600.a.A1.GE32590	Full length( MytuD.17600.a )		1	469

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|83332.12.peg.3134
RefSeq:	NP_217328.1
TubercuList:	Rv2812
MTB Network Portal:	Rv2812
UniProt:	P71639

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAVGDDEEKV RAERARAIGL FRYQLIWEAA DAAHSTKQRG KMVRELASRE HTDPFGRRVR ISRQTIDRWI RGWRAGGFDA LVPNPRQCTP RTPAEVLELA VALRRENPQR TAAAIRRILR TQLGWAPDER TLQRNFHRLG LTGATTGSAP AVFGRFEAEH PNALWTGDVL HGIRIDLRKT YLFAFLDDHS RLVPGYRWGH AEDTVRLAAA LRPALASRGV PNAVYVDNGS PYVDAWLLRA CAKLGVRLVH STPGRPQGRG KIERFFRTVR EQFLVEITGE PDVVGRHYVA DLAELNRLFT AWVETVYHRS VHSETGQTPL ARWSAGGPIP LPAPETLTEA FLWEEHRRVT KTATVSLHGN RYEIDPALVG RKVELVFDPF DLTRIEVRLA GAPMRRAIPY HIGRHSHPKA KPETPTAPPK PSGIDYAQLI ETAHAAELAR GVNYTALTGA ADQIPGQLDL LTGQEAQPK

NT Sequence

GTGGCGGTCG GCGATGACGA GGAGAAGGTG CGCGCGGAGC GCGCGAGGGC GATCGGGTTG TTTCGCTACC AGTTGATTTG GGAGGCCGCC GATGCGGCGC ATTCCACCAA GCAGCGGGGA AAGATGGTGC GCGAGTTGGC CTCACGCGAG CACACCGATC CGTTCGGGCG GCGGGTGCGC ATCAGCCGCC AAACCATCGA CCGCTGGATC CGGGGCTGGC GGGCCGGCGG GTTCGACGCG CTGGTGCCCA ACCCACGCCA GTGCACACCG CGTACCCCGG CCGAGGTGCT GGAGCTGGCG GTGGCGCTGC GGCGGGAAAA CCCGCAGCGC ACGGCGGCGG CAATCCGGCG GATCCTGCGT ACCCAGTTGG GCTGGGCGCC CGATGAACGC ACCCTGCAAC GCAACTTCCA CCGGCTCGGG CTCACCGGCG CCACCACCGG GTCGGCGCCG GCGGTGTTCG GCCGGTTCGA AGCCGAGCAC CCGAACGCCC TGTGGACCGG GGATGTGTTG CACGGCATAC GGATTGATCT CCGCAAGACC TATCTGTTCG CGTTCTTAGA CGACCATTCC CGGTTGGTGC CCGGCTACCG GTGGGGCCAT GCCGAGGACA CGGTGCGGCT GGCCGCCGCA CTGCGCCCGG CGCTGGCCTC CCGCGGCGTG CCCAACGCGG TGTATGTCGA TAACGGCTCG CCCTATGTGG ATGCGTGGTT GTTGCGGGCA TGCGCGAAAC TCGGTGTGCG CCTTGTTCAT TCCACGCCAG GTCGGCCGCA AGGCAGGGGC AAGATAGAGA GGTTCTTCCG CACCGTGCGC GAGCAGTTCC TGGTCGAGAT CACCGGCGAA CCCGACGTCG TCGGCCGACA TTACGTCGCT GATCTGGCCG AGTTGAATCG GCTGTTTACG GCCTGGGTCG AAACGGTTTA TCACCGCAGC GTGCATTCCG AAACCGGGCA GACCCCGCTG GCCCGCTGGT CAGCCGGCGG CCCCATCCCG CTGCCCGCCC CCGAGACGCT CACCGAGGCC TTCCTGTGGG AGGAGCACCG CCGCGTGACC AAGACCGCCA CCGTCTCGCT GCACGGCAAC CGCTACGAGA TCGACCCGGC GCTGGTCGGC CGGAAAGTGG AGTTGGTGTT CGACCCGTTC GATTTGACCC GCATCGAGGT GCGGCTGGCC GGCGCGCCGA TGAGGCGGGC CATTCCGTAT CACATCGGGC GCCATTCACA CCCGAAAGCC AAACCCGAAA CCCCCACCGC ACCGCCCAAA CCCAGCGGCA TCGACTACGC GCAGTTAATC GAGACCGCGC ACGCAGCCGA ACTCGCCCGC GGCGTCAACT ACACCGCCCT CACCGGGGCT GCCGATCAGA TCCCCGGCCA GCTCGACCTG CTCACCGGCC AGGAGGCCCA ACCGAAA

Details for MytuD.17600.a.A1.GE32590

HARVESTED ON:	5/17/2011
SEQUENCED ON:	5/23/2011
EXPECTED MW:	54kDa
OBSERVED MW:	54kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVAVGDDEE KVRAERARAI GLFRYQLIWE AADAAHSTKQ RGKMVRELAS REHTDPFGRR VRISRQTIDR WIRGWRAGGF DALVPNPRQC TPRTPAEVLE LAVALRRENP QRTAAAIRRI LRTQLGWAPD ERTLQRNFHR LGLTGATTGS APAVFGRFEA EHPNALWTGD VLHGIRIDLR KTYLFAFLDD HSRLVPGYRW GHAEDTVRLA AALRPALASR GVPNAVYVDN GSPYVDAWLL RACAKLGVRL VHSTPGRPQG RGKIERFFRT VREQFLVEIT GEPDVVGRHY VADLAELNRL FTAWVETVYH RSVHSETGQT PLARWSAGGP IPLPAPETLT EAFLWEEHRR VTKTATVSLH GNRYEIDPAL VGRKVELVFD PFDLTRIEVR LAGAPMRRAI PYHIGRHSHP KAKPETPTAP PKPSGIDYAQ LIETAHAAEL ARGVNYTALT GAADQIPGQL DLLTGQEAQP K

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgg cggtcggcga tgacgaggag aaggtgcgcg cggagcgcgc gagggcgatc gggttgtttc gctaccagtt gatttgggag gccgccgatg cggcgcattc caccaagcag cggggaaaga tggtgcgcga gttggcctca cgcgagcaca ccgatccgtt cgggcggcgg gtgcgcatca gccgccaaac catcgaccgc tggatccggg gctggcgggc cggcgggttc gacgcgctgg tgcccaaccc acgccagtgc acaccgcgta ccccggccga ggtgctggag ctggcggtgg cgctgcggcg ggaaaacccg cagcgcacgg cggcggcaat ccggcggatc ctgcgtaccc agttgggctg ggcgcccgat gaacgcaccc tgcaacgcaa cttccaccgg ctcgggctca ccggcgccac caccgggtcg gcgccggcgg tgttcggccg gttcgaagcc gagcacccga acgccctgtg gaccggggat gtgttgcacg gcatacggat tgatctccgc aagacctatc tgttcgcgtt cttagacgac cattcccggt tggtgcccgg ctaccggtgg ggccatgccg aggacacggt gcggctggcc gccgcactgc gcccggcgct ggcctcccgc ggcgtgccca acgcggtgta tgtcgataac ggctcgccct atgtggatgc gtggttgttg cgggcatgcg cgaaactcgg tgtgcgcctt gttcattcca cgccaggtcg gccgcaaggc aggggcaaga tagagaggtt cttccgcacc gtgcgcgagc agttcctggt cgagatcacc ggcgaacccg acgtcgtcgg ccgacattac gtcgctgatc tggccgagtt gaatcggctg tttacggcct gggtcgaaac ggtttatcac cgcagcgtgc attccgaaac cgggcagacc ccgctggccc gctggtcagc cggcggcccc atcccgctgc ccgcccccga gacgctcacc gaggccttcc tgtgggagga gcaccgccgc gtgaccaaga ccgccaccgt ctcgctgcac ggcaaccgct acgagatcga cccggcgctg gtcggccgga aagtggagtt ggtgttcgac ccgttcgatt tgacccgcat cgaggtgcgg ctggccggcg cgccgatgag gcgggccatt ccgtatcaca tcgggcgcca ttcacacccg aaagccaaac ccgaaacccc caccgcaccg cccaaaccca gcggcatcga ctacgcgcag ttaatcgaga ccgcgcacgc agccgaactc gcccgcggcg tcaactacac cgccctcacc ggggctgccg atcagatccc cggccagctc gacctgctca ccggccagga ggcccaaccg aaata

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggcggtc ggcgatgacg aggagaaggt gcgcgcggag cgcgcgaggg cgatcgggtt gtttcgctac cagttgattt gggaggccgc cgatgcggcg cattccacca agcagcgggg aaagatggtg cgcgagttgg cctcacgcga gcacaccgat ccgttcgggc ggcgggtgcg catcagccgc caaaccatcg accgctggat ccggggctgg cgggccggcg ggttcgacgc gctggtgccc aacccacgcc agtgcacacc gcgtaccccg gccgaggtgc tggagctggc ggtggcgctg cggcgggaaa acccgcagcg cacggcggcg gcaatccggc ggatcctgcg tacccagttg ggctgggcgc ccgatgaacg caccctgcaa cgcaacttcc accggctcgg gctcaccggc gccaccaccg ggtcggcgcc ggcggtgttc ggccggttcg aagccgagca cccgaacgcc ctgtggaccg gggatgtgtt gcacggcata cggattgatc tccgcaagac ctatctgttc gcgttcttag acgaccattc ccggttggtg cccggctacc ggtggggcca tgccgaggac acggtgcggc tggccgccgc actgcgcccg gcgctggcct cccgcggcgt gcccaacgcg gtgtatgtcg ataacggctc gccctatgtg gatgcgtggt tgttgcgggc atgcgcgaaa ctcggtgtgc gccttgttca ttccacgcca ggtcggccgc aaggcagggg caagatagag aggttcttcc gcaccgtgcg cgagcagttc ctggtcgaga tcaccggcga acccgacgtc gtcggccgac attacgtcgc tgatctggcc gagttgaatc ggctgtttac ggcctgggtc gaaacggttt atcaccgcag cgtgcattcc gaaaccgggc agaccccgct ggcccgctgg tcagccggcg gccccatccc gctgcccgcc cccgagacgc tcaccgaggc cttcctgtgg gaggagcacc gccgcgtgac caagaccgcc accgtctcgc tgcacggcaa ccgctacgag atcgacccgg cgctggtcgg ccggaaagtg gagttggtgt tcgacccgtt cgatttgacc cgcatcgagg tgcggctggc cggcgcgccg atgaggcggg ccattccgta tcacatcggg cgccattcac acccgaaagc caaacccgaa acccccaccg caccgcccaa acccagcggc atcgactacg cgcagttaat cgagaccgcg cacgcagccg aactcgcccg cggcgtcaac tacaccgccc tcaccggggc tgccgatcag atccccggcc agctcgacct gctcaccggc caggaggccc aaccgaaata aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a