MytuD.17590.a

Putative uncharacterized protein

CENTER ID: MytuD.17590.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.17590.a.A1.GE30999 Full length( MytuD.17590.a ) 1 302
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.471
RefSeq: NP_214942.1
TubercuList: Rv0428c
MTB Network Portal: Rv0428c
UniProt: P96274

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVSWPGLGTR VTVRYRRPAG SMPPLTDAVG RLLAVDPTVR VQTKTGTIVE FSPVDVVALR VLTDAPVRTA AIRALEHAAA AAWPGVERTW LDGWLLRAGH GAVLAANSAV PLDISAHTNT ITEISAWYAS RDLQPWLAVP DRLLPLPADL AGERREQVLV RDVSTGEPDR SVTLLDHPDD TWLRLYHQRL PLDMATPVID GELAFGSYLG VAVARAAVTD APDGTRWVGL SAMRAADEQS ATGSAGRQLW EALLGWGAGR GATRGYVRVH DTATSVLAES LGFRLHHHCR YLPAQSVGWD TF
NT Sequence
ATGGTCTCGT GGCCGGGGCT TGGGACACGG GTAACGGTGC GCTACCGCCG GCCCGCCGGA TCGATGCCAC CGCTGACCGA TGCGGTGGGT CGGCTGCTAG CCGTCGATCC GACGGTGCGA GTGCAGACCA AGACCGGGAC CATCGTCGAG TTCTCGCCCG TCGATGTGGT GGCCCTGCGG GTCCTGACCG ATGCGCCGGT GCGCACCGCC GCGATCCGCG CACTTGAACA CGCCGCCGCG GCGGCCTGGC CCGGCGTTGA ACGAACCTGG CTGGACGGCT GGCTCCTGCG AGCCGGACAC GGGGCCGTAC TGGCCGCTAA TTCAGCAGTA CCGCTGGATA TCTCGGCACA CACGAACACC ATCACAGAAA TCAGCGCATG GTATGCGAGC CGTGATCTGC AGCCATGGCT GGCCGTCCCG GACCGCTTGC TGCCACTACC GGCCGACCTG GCAGGTGAGC GCCGAGAGCA AGTGTTGGTG CGTGACGTGT CCACCGGTGA ACCCGACCGG TCGGTCACGT TGCTGGACCA CCCCGACGAC ACCTGGCTGC GGCTCTACCA CCAACGCCTA CCCCTCGACA TGGCGACCCC CGTCATCGAC GGCGAGTTGG CATTCGGCAG CTACCTGGGT GTGGCGGTCG CACGTGCAGC GGTAACCGAC GCACCCGACG GCACCCGGTG GGTGGGTCTG TCGGCAATGC GTGCCGCCGA CGAGCAATCC GCGACCGGCT CCGCTGGCCG CCAATTGTGG GAAGCGCTGT TGGGCTGGGG AGCGGGCCGC GGCGCGACCC GCGGCTACGT GCGCGTGCAC GACACCGCGA CATCCGTCCT CGCCGAGTCC CTGGGCTTCC GGCTGCACCA TCACTGCCGC TATCTTCCTG CCCAGTCGGT CGGCTGGGAT ACCTTC
Details for MytuD.17590.a.A1.GE30999
HARVESTED ON: 12/14/2010
SEQUENCED ON: 12/20/2010
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMVSWPGL GTRVTVRYRR PAGSMPPLTD AVXRLLAVDP TVRVQTKTGT IVEFSPVDVV ALRVLTDAPV RTAAIRALEH AAAAAWPGVE RTWLDGWLLR AGHGAVLAAN SAVPLDISAH TNTITEISAW YASRDLQPWL AVPDRLLPLP ADLAGERREQ VLVRDVSTGE PDRSVTLLDH PDDTWLRLYH QRLPLDMATP VIDGELAFGS YLGVAVARAA VTDAPDGTRW VGLSAMRAAD EQSATGSAGR QLWEALLGWG AGRGATRGYV RVHDTATSVL AESLGFRLHH HCRYLPAQSV GWDTF
Validated NT Sequence
ttattagaag gtatcccagc cgaccgactg ggcaggaaga tagcggcagt gatggtgcag ccggaagccc agggactcgg cgaggacgga tgtcgcggtg tcgtgcacgc gcacgtagcc gcgggtcgcg ccgcggcccg ctccccagcc caacagcgct tcccacaatt ggcggccagc ggagccggtc gcggattgct cgtcggcggc acgcattgcc gacagaccca cccaccgggt gccgtcgggt gcgtcggtta ccgctgcacg tgcgaccgcc acacccaggt agctgccgaa tgccaactcg ccgtcgatga cgggggtcgc catgtcgagg ggtaggcgtt ggtggtagag ccgcagccag gtgtcgtcgg ggtggtccag caacgtgacc gaccggtcgg gttcaccggt ggacacgtca cgcaccaaca cttgctctcg gcgctcacct gccaggtcgg ccggtagtgg cagcaagcgg tccgggacgg ccagccatgg ctgcagatca cggctcgcat accatgcgct gatttctgtg atggtgttcg tgtgtgccga gatatccagc ggtactgctg aattagcggc cagtacggcc ccgtgtccgg ctcgcaggag ccagccgtcc agccaggttc gttcaacgcc gggccaggcc gccgcggcgg cgtgttcaag tgcgcggatc gcggcggtgc gcaccggcgc atcggtcagg acccgcaggg ccaccacatc gacgggcgag aactcgacga tggtcccggt cttggtctgc actcgcaccg tcggatcgac ggctagcagc cganccaccg catcggtcag cggtggcatc gatccggcgg gccggcggta gcgcaccgtt acccgtgtcc caagccccgg ccacgagacc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSWPGLGT RVTVRYRRPA GSMPPLTDAV GRLLAVDPTV RVQTKTGTIV EFSPVDVVAL RVLTDAPVRT AAIRALEHAA AAAWPGVERT WLDGWLLRAG HGAVLAANSA VPLDISAHTN TITEISAWYA SRDLQPWLAV PDRLLPLPAD LAGERREQVL VRDVSTGEPD RSVTLLDHPD DTWLRLYHQR LPLDMATPVI DGELAFGSYL GVAVARAAVT DAPDGTRWVG LSAMRAADEQ SATGSAGRQL WEALLGWGAG RGATRGYVRV HDTATSVLAE SLGFRLHHHC RYLPAQSVGW DTF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtctcgtgg ccggggcttg ggacacgggt aacggtgcgc taccgccggc ccgccggatc gatgccaccg ctgaccgatg cggtgggtcg gctgctagcc gtcgatccga cggtgcgagt gcagaccaag accgggacca tcgtcgagtt ctcgcccgtc gatgtggtgg ccctgcgggt cctgaccgat gcgccggtgc gcaccgccgc gatccgcgca cttgaacacg ccgccgcggc ggcctggccc ggcgttgaac gaacctggct ggacggctgg ctcctgcgag ccggacacgg ggccgtactg gccgctaatt cagcagtacc gctggatatc tcggcacaca cgaacaccat cacagaaatc agcgcatggt atgcgagccg tgatctgcag ccatggctgg ccgtcccgga ccgcttgctg ccactaccgg ccgacctggc aggtgagcgc cgagagcaag tgttggtgcg tgacgtgtcc accggtgaac ccgaccggtc ggtcacgttg ctggaccacc ccgacgacac ctggctgcgg ctctaccacc aacgcctacc cctcgacatg gcgacccccg tcatcgacgg cgagttggca ttcggcagct acctgggtgt ggcggtcgca cgtgcagcgg taaccgacgc acccgacggc acccggtggg tgggtctgtc ggcaatgcgt gccgccgacg agcaatccgc gaccggctcc gctggccgcc aattgtggga agcgctgttg ggctggggag cgggccgcgg cgcgacccgc ggctacgtgc gcgtgcacga caccgcgaca tccgtcctcg ccgagtccct gggcttccgg ctgcaccatc actgccgcta tcttcctgcc cagtcggtcg gctgggatac cttctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa