SSGCID: Target Page

MytuD.17568.a

PROBABLE LIPASE LIPD

CENTER ID:	MytuD.17568.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.17568.a.A1.GE32589	Full length( MytuD.17568.a )		1	446

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|83332.12.peg.2149
RefSeq:	NP_216439.1
TubercuList:	Rv1923
MTB Network Portal:	Rv1923
UniProt:	P95290

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDVAGLPRLA AGTQAAIIHG MAQPPSLLTT DNGLPFGVQG ACDSRFTGVI RAFAGLYPGR KFGGGALSVY IDGRQVVDVW TGWSDRQGKV PWTADTGAMV FSATKGLAAT VIHRLVDRGL LSYDAPVAEY WPEFGANGKS EVTVSDVLRH RSGLAHLKGV DKDEVMDHLL MEQKLAAAPL DRQHGKLAYH AVTYGWLLSG LARAVTGKGM RELFREELAR PLNTDGIHLG RPPADSPTKA AQTLLPQAKV PTPLLDFIAP KVAGLSFSGL LGAVYFPGIL SLLQDDMPFL DGEVPAVNGV VTARALAKTY GALANDGVID GTRLLSSQAV RGLTGKSELW PDLNLGLPFT YHQGYQSSPV PGLLEGYGHI GLGGTIGWAD PETGSAFGYV HNRLLTLLLF DIGSFAGLAA LLNSAVVAAR RDDPLEVPHF GAPYSEPRHE QAASGA

NT Sequence

TTGGATGTAG CGGGGCTACC GCGGTTGGCC GCCGGTACCC AGGCTGCAAT CATTCACGGT ATGGCGCAAC CACCGTCACT CCTCACAACT GACAATGGCC TACCCTTCGG CGTGCAAGGT GCCTGCGACT CCCGTTTCAC CGGAGTCATC CGTGCCTTTG CTGGGCTGTA CCCCGGCCGC AAGTTCGGGG GTGGGGCACT GTCGGTTTAT ATCGACGGTC GCCAGGTCGT CGATGTCTGG ACGGGGTGGT CCGATCGGCA GGGCAAAGTA CCCTGGACGG CCGATACCGG GGCAATGGTG TTCTCCGCGA CCAAAGGGTT GGCCGCAACG GTGATTCACC GTTTGGTCGA TCGCGGCCTT TTGTCCTACG ACGCGCCGGT CGCGGAGTAC TGGCCCGAGT TCGGAGCTAA CGGCAAGTCT GAGGTCACCG TCAGCGATGT GTTGCGACAT CGGTCCGGAC TGGCGCACCT CAAGGGGGTG GACAAGGACG AGGTCATGGA CCACCTCCTG ATGGAGCAGA AGTTGGCGGC TGCGCCGCTA GACCGCCAGC ACGGGAAGTT GGCTTACCAT GCGGTGACTT ACGGATGGCT GCTGTCCGGC TTGGCTCGTG CAGTGACCGG CAAAGGCATG CGTGAACTGT TCCGCGAAGA ACTCGCTCGC CCGCTGAACA CCGATGGTAT TCATCTCGGC CGGCCACCGG CCGACTCGCC TACCAAGGCG GCACAGACAC TTCTGCCCCA AGCCAAGGTC CCCACCCCAC TGCTCGATTT CATCGCACCA AAGGTTGCGG GGCTGTCGTT CTCCGGGCTG CTCGGCGCCG TCTACTTCCC GGGCATCCTG TCGTTGCTGC AAGACGATAT GCCGTTCCTC GACGGTGAGG TTCCGGCGGT CAACGGCGTT GTGACCGCGC GCGCCCTGGC CAAGACGTAT GGGGCGTTGG CCAATGACGG TGTGATCGAC GGCACCCGAC TGCTGTCGTC GCAGGCGGTA CGTGGATTGA CGGGGAAGTC CGAGCTATGG CCGGACCTTA ATCTCGGTCT TCCTTTTACC TACCACCAGG GTTACCAATC GTCTCCGGTG CCTGGGCTGC TGGAGGGGTA CGGCCACATC GGGCTCGGTG GCACGATCGG ATGGGCCGAC CCGGAGACCG GCAGCGCATT CGGATATGTG CATAACCGCT TGCTGACGCT ACTGTTGTTC GATATTGGCT CGTTCGCAGG GCTGGCTGCG CTGCTGAACA GCGCCGTCGT GGCAGCACGT CGCGATGACC CCCTGGAAGT GCCGCATTTC GGTGCGCCCT ATAGCGAACC GCGTCATGAG CAGGCGGCCT CGGGTGCA

Details for MytuD.17568.a.A1.GE32589

HARVESTED ON:	5/17/2011
SEQUENCED ON:	12/5/2014
EXPECTED MW:	49kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLDVAGLPR LAAGTQAAII HGMAQPPSLL TTDNGLPFGV QGACDSRFTG VIRAFAGLYP GRKFGGGALS VYIDGRQVVD VWTGWSDRQG KVPWTADTGA MVFSATKGLA ATVIHRLVDR GLLSYDAPVA EYWPEFGANG KSEVTVSDVL RHRSGLAHLK GVDKDEVMDH LLMEQKLAAA PLDRQHGKLA YHAVTYGWLL SGLARAVTGK GMRELFREEL ARPLNTDGIH LGRPPADSPT KAAQTLLPQA KVPTPLLDFI APKVAGLSFS GLLGAVYFPG ILSLLQDDMP FLDGEVPAVN GVVTARALAK TYGALANDGV IDGTRLLSSQ AVRGLTGKSE LWPDLNLGLP FTYHQGYQSS PVPGLLEGYG HIGLGGTIGW ADPETGSAFG YVHNRLLTLL LFDIGSFAGL AALLNSAVVA ARRDDPLEVP HFGAPYSEPR HEQAASGA

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttgg atgtagcggg gctaccgcgg ttggccgccg gtacccaggc tgcaatcatt cacggtatgg cgcaaccacc gtcactcctc acaactgaca atggcctacc cttcggcgtg caaggtgcct gcgactcccg tttcaccgga gtcatccgtg cctttgctgg gctgtacccc ggccgcaagt tcgggggtgg ggcactgtcg gtttatatcg acggtcgcca ggtcgtcgat gtctggacgg ggtggtccga tcggcagggc aaagtaccct ggacggccga taccggggca atggtgttct ccgcgaccaa agggttggcc gcaacggtga ttcaccgttt ggtcgatcgc ggccttttgt cctacgacgc gccggtcgcg gagtactggc ccgagttcgg agctaacggc aagtctgagg tcaccgtcag cgatgtgttg cgacatcggt ccggactggc gcacctcaag ggggtggaca aggacgaggt catggaccac ctcctgatgg agcagaagtt ggcggctgcg ccgctagacc gccagcacgg gaagttggct taccatgcgg tgacttacgg atggctgctg tccggcttgg ctcgtgcagt gaccggcaaa ggcatgcgtg aactgttccg cgaagaactc gctcgcccgc tgaacaccga tggtattcat ctcggccggc caccggccga ctcgcctacc aaggcggcac agacacttct gccccaagcc aaggtcccca ccccactgct cgatttcatc gcaccaaagg ttgcggggct gtcgttctcc gggctgctcg gcgccgtcta cttcccgggc atcctgtcgt tgctgcaaga cgatatgccg ttcctcgacg gtgaggttcc ggcggtcaac ggcgttgtga ccgcgcgcgc cctggccaag acgtatgggg cgttggccaa tgacggtgtg atcgacggca cccgactgct gtcgtcgcag gcggtacgtg gattgacggg gaagtccgag ctatggccgg accttaatct cggtcttcct tttacctacc accagggtta ccaatcgtct ccggtgcctg ggctgctgga ggggtacggc cacatcgggc tcggtggcac gatcggatgg gccgacccgg agaccggcag cgcattcgga tatgtgcata accgcttgct gacgctactg ttgttcgata ttggctcgtt cgcagggctg gctgcgctgc tgaacagcgc cgtcgtggca gcacgtcgcg atgaccccct ggaagtgccg catttcggtg cgccctatag cgaaccgcgt catgagcagg cggcctcggg tgcataat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttggatgta gcggggctac cgcggttggc cgccggtacc caggctgcaa tcattcacgg tatggcgcaa ccaccgtcac tcctcacaac tgacaatggc ctacccttcg gcgtgcaagg tgcctgcgac tcccgtttca ccggagtcat ccgtgccttt gctgggctgt accccggccg caagttcggg ggtggggcac tgtcggttta tatcgacggt cgccaggtcg tcgatgtctg gacggggtgg tccgatcggc agggcaaagt accctggacg gccgataccg gggcaatggt gttctccgcg accaaagggt tggccgcaac ggtgattcac cgtttggtcg atcgcggcct tttgtcctac gacgcgccgg tcgcggagta ctggcccgag ttcggagcta acggcaagtc tgaggtcacc gtcagcgatg tgttgcgaca tcggtccgga ctggcgcacc tcaagggggt ggacaaggac gaggtcatgg accacctcct gatggagcag aagttggcgg ctgcgccgct agaccgccag cacgggaagt tggcttacca tgcggtgact tacggatggc tgctgtccgg cttggctcgt gcagtgaccg gcaaaggcat gcgtgaactg ttccgcgaag aactcgctcg cccgctgaac accgatggta ttcatctcgg ccggccaccg gccgactcgc ctaccaaggc ggcacagaca cttctgcccc aagccaaggt ccccacccca ctgctcgatt tcatcgcacc aaaggttgcg gggctgtcgt tctccgggct gctcggcgcc gtctacttcc cgggcatcct gtcgttgctg caagacgata tgccgttcct cgacggtgag gttccggcgg tcaacggcgt tgtgaccgcg cgcgccctgg ccaagacgta tggggcgttg gccaatgacg gtgtgatcga cggcacccga ctgctgtcgt cgcaggcggt acgtggattg acggggaagt ccgagctatg gccggacctt aatctcggtc ttccttttac ctaccaccag ggttaccaat cgtctccggt gcctgggctg ctggaggggt acggccacat cgggctcggt ggcacgatcg gatgggccga cccggagacc ggcagcgcat tcggatatgt gcataaccgc ttgctgacgc tactgttgtt cgatattggc tcgttcgcag ggctggctgc gctgctgaac agcgccgtcg tggcagcacg tcgcgatgac cccctggaag tgccgcattt cggtgcgccc tatagcgaac cgcgtcatga gcaggcggcc tcgggtgcat aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa